Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,388,041	T→G	T50P (ACG→CCG)	aaeB ←	aromatic carboxylic acid efflux pump subunit AaeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,388,041	0	T	G	100.0%	200.0 / NA	61	T50P (ACG→CCG)	aaeB	aromatic carboxylic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (29/32);  total (29/32)

TGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGA‑GTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAAGCGAATATGTT‑GGTTAGCAATGGAGAAAATA‑CCCATCGTGATTAACCAAACTCACGCAGGCGATGGG‑CCATTTTACGGAAGAACGAATCC‑TGGCTTTCGTCGCGATCTTGTTTGCCAGTGA  >  NC_000913/3387798‑3388273                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               agATCTGCGCGGTAGACCCGTCCAGCCCCCACGCATACGATGTTTT‑TATTCGTACCAGCGAGGATATCCAGGTGCAAAACCCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAAGACAGCCAATAAATGTGTCTATGATGCGCAAAAAGCCACGAGAGCGAATAGCGCCAGAGTACGGTTCACCTCCCGCAGCAAATGCCGGACcggcg                                                                                                                                                                                                                                         <  2:313401/250‑1 (MQ=255)    tCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGA‑GTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCggcggc                                                                                                                                                                                                                                       >  1:22394/1‑250 (MQ=255)           ggTATAACCGGCCAGCCCCCACGCATACGA‑GTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGTCAACAATcg                                                                                                                                                                                                                               <  2:488298/251‑1 (MQ=255)                aaCCGGCCAGCCCCCACGCATACGA‑GTGTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCAGCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATCCAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGTTTGCGAATAGCGCCAGAATACGTTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATcgccgc                                                                                                                                                                                                                           <  1:515626/250‑1 (MQ=255)                    ggCCAGCCCCCACGCATACGA‑GTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTcc                                                                                                                                                                                                                      >  2:267468/1‑250 (MQ=255)                    ggCCAGCCCCCACGCATACGA‑GTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTc                                                                                                                                                                                                                       >  1:398616/1‑250 (MQ=255)                       cAGCCCCCACGCATACGA‑GTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTa                                                                                                                                                                                                                   <  2:139416/251‑1 (MQ=255)                           ccccACGCATACGA‑GTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGAGCAAAAAGCCCCGATAGAGACTAGCGCAAGAATACGGTTAACCTCCCGCAGCAAAGTCCGGACAGGCGGCCACAATCGCCGCGGTCAGTAcacc                                                                                                                                                                                                               >  2:159510/1‑248 (MQ=255)                               aCGCATACGA‑GTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCAACAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTGAGTACCGCCCAg                                                                                                                                                                                                           <  2:52218/251‑1 (MQ=255)                                cGCATACGA‑GTTTTCTATTCGGACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGc                                                                                                                                                                                                          <  2:398616/251‑1 (MQ=255)                                   aTACGA‑GTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGGCAGTACCGCCCAGCGTg                                                                                                                                                                                                       <  1:394554/251‑1 (MQ=255)                                                        ccAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGCATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAg                                                                                                                                                                                      >  2:268113/1‑248 (MQ=255)                                                           gCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAg                                                                                                                                                                                <  2:396171/251‑1 (MQ=255)                                                                   atCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGCCAGTACCGCCCCGCGGGGCGTTTCCAGCTGGAAGTGAAAGCcaacaa                                                                                                                                                                         >  2:264577/1‑250 (MQ=255)                                                                    tCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCaacaaac                                                                                                                                                                       >  1:444152/1‑251 (MQ=255)                                                                      cAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCaacaaac                                                                                                                                                                       >  2:112309/1‑249 (MQ=255)                                                                          tACAAAAACCGGCCCAGATACAGCACACCAGAATAATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCAAGCTGGAAGTGAAAGCCAACAAACAGCGcc                                                                                                                                                                 >  1:485383/1‑251 (MQ=255)                                                                           aCAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGcc                                                                                                                                                                 <  1:551960/250‑1 (MQ=255)                                                                                aaCCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACa                                                                                                                                                           <  1:268113/251‑1 (MQ=255)                                                                                    ggCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATgg                                                                                                                                                       >  1:284775/1‑251 (MQ=255)                                                                                           aTACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGgcgc                                                                                                                                                >  1:238620/1‑251 (MQ=255)                                                                                                  acacCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGGGTCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTcccccggttt                                                                                                                                         >  2:179497/1‑242 (MQ=255)                                                                                                      cAGAATCATCAATAATGGTGCGCGGATCATCGCAAGGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCg                                                                                                                                    <  2:121812/251‑1 (MQ=255)                                                                                                       aGAATCATCAATAATGGGGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATAGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGc                                                                                                                                   >  2:410152/1‑251 (MQ=255)                                                                                                          atcatcAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑aaa                                                                                                                               <  1:53437/251‑1 (MQ=255)                                                                                                           acatcaATAATGGTGCGCGGATCATCGCAATGAGGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAAg                                                                                                                              <  2:481777/250‑1 (MQ=255)                                                                                                            catcaATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAAGc                                                                                                                             <  2:287928/251‑1 (MQ=255)                                                                                                                     aggTGCGCGGATCATCGCAAGGATGATCACCAGTCGGGGAATACAGCCAATAAATGTGCCGAGGATGCGCAAAAAGCCGCGATAGCGAATAGCGCCAGAATACGGTTCCCCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAAGCGCCGCTGTCAGTACCGTCTAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATTGCTGTTGCC‑GGTTTTACCGT‑AGAGCGAATATGtt                                                                                                                    <  2:210067/250‑1 (MQ=255)                                                                                                                           gcgGATCATCGCAAGGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTGTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAAGCGAATATGTT‑GGTTAg                                                                                                             <  2:392121/251‑1 (MQ=255)                                                                                                                           gcgGAACATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAAGCGAATATGTT‑GGTTAg                                                                                                             <  2:419820/251‑1 (MQ=255)                                                                                                                             ggATCATCGCAATGATGATCCCCAGTCCGGCAAGACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAAGAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAAGCGAATATGTT‑GGTTAGCa                                                                                                           <  1:64919/251‑1 (MQ=255)                                                                                                                             ggATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATTGCGGACGCC‑CGTTTTACCCC‑AAAGCGAATATGTT‑GGTTAGc                                                                                                            >  2:282956/1‑250 (MQ=255)                                                                                                                                          gatgatTACCAGTCCGGCAATATAGCTTATAAATGTGCCTATGATGCGCAAAAAGCCACGATATCGAATAGCGCCCGAATTCGGTGCACTTCCCGCAGCAAAGGCCGGACCGGCGGCAACATGCGCCGCTGTCAGTTGCGCCCAGCTTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAAGCAAATATGTT‑GGTTAGCAATGGAGAAAATA‑c                                                                                             <  1:534626/251‑1 (MQ=255)                                                                                                                                          gatgatCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTTCCGC‑CAAGCCAATATGTT‑GGTTAGCCATGGAGAAAATA‑c                                                                                             >  1:4077/1‑251 (MQ=255)                                                                                                                                           atgatCACCAGACCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCCAGTTT‑AACGC‑CAAACGAAAATGTG‑GGTTAGCAAAGGAGAAAATaac                                                                                             >  2:402001/1‑249 (MQ=255)                                                                                                                                             gatCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCACAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTCCACTGGCGGTCGCC‑AGTTTTACCCC‑AAAGCGGATTAGTT‑GGGTCGCAATGGAGAAAATA‑cccc                                                                                          >  2:368316/1‑250 (MQ=255)                                                                                                                                             gatCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAAGCGAATATGTT‑GGTTAGCAATGGAGAAAATA‑CCCa                                                                                          <  1:287591/251‑1 (MQ=255)                                                                                                                                             gatCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAAGAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAAGCGAATATGGT‑GGTTAGCAATGGAGAAAATA‑ccc                                                                                           <  2:385033/250‑1 (MQ=255)                                                                                                                                                  ccAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑CAAGCGAATATGTT‑TGTTTGCCATGGGGAAAAAA‑CCCATCGTg                                                                                     >  2:9435/1‑251 (MQ=255)                                                                                                                                                  ccAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAAGCGAATATGTT‑GGTTAGCAATGGAGAAAATA‑CCCATCGTg                                                                                     <  1:11559/251‑1 (MQ=255)                                                                                                                                                       ccGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAAGCGAATATGTT‑GGTTAGCAATGGGGAAAATA‑CCCATCGTGATTa                                                                                 <  2:37982/250‑1 (MQ=255)                                                                                                                                                           cAATACAGCAAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGACTAGCGCCAGAATCCGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGACAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGGCGGC‑AGTTTTACCGC‑AAAACGAAAATGGT‑GGTTAGCAAAGGGGAAAAAAACCCA‑CGGGATTAACCa                                                                             >  2:360778/1‑250 (MQ=255)                                                                                                                                                                  gCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAAGCGAATATGTT‑GGTTAGCAATGGAGAAAATA‑CCCATCGTGATTAACCAAACTCACg                                                                     <  2:109716/251‑1 (MQ=255)                                                                                                                                                                      aTAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAATCGAATATGTT‑GGTTAGCAATGGAGAAAATA‑CCCATCGTGATTAACCAAACTCTCGCAgg                                                                 <  2:66062/251‑1 (MQ=255)                                                                                                                                                                                cGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑CGATTTACCGCCAAAG‑GAAAATTTT‑GGTTTGCAATGGGGGAAATA‑ACCCTCGGGATTAAACACACTCACGCAGGCGCTGGG‑ccc                                                      >  2:1001/1‑250 (MQ=255)                                                                                                                                                                                 gatgatGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAAACGAATATGTT‑GGGTAGCAATGGGGAAAATA‑CCCCTCGTGATTAACCAAACTCACGCAGGCGATGGG‑GCAt                                                     >  2:79511/1‑251 (MQ=255)                                                                                                                                                                                          cAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑CGGTTTACCGC‑CAAGCGCATATGTT‑GGTTAGCAATGGAGAAAATA‑ACCATCGTGATTAACCAAACTTACGTAGGCGGTGGG‑CCATTTTACggg                                             >  2:32964/1‑249 (MQ=255)                                                                                                                                                                                                   aCGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAAGCGAATATGTT‑GGTTAGCAATGGAGAAAATA‑CCCATCGTGGTTAACCAAACTCACGCAGGCGATGGG‑GCATTTTACGGAAGAACGAATc                                   >  1:437226/1‑251 (MQ=255)                                                                                                                                                                                                   aCGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGGGAAAGCCAACAAACAGCGCCAGTACAATGGCGGGCCCC‑CGTTTTTCCGC‑CAAACGGAAATGTTTGGTT‑GGAATGGGGAAAATA‑ACCATCCGGATTTACCAAACACACCCAGGCGGTGGGGCCATTTTA‑GGAAGAAccaaac                                   >  2:150827/1‑246 (MQ=255)                                                                                                                                                                                                        aGCGAATAGCGCCAGAATACAGTTCGCCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAACCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAAGCGAATAGGTT‑GGTTAGCAATGGAGAAAATA‑CCCATCGTGATTAACCAAACTCACGCAGGCGATGGG‑CCATTTTACGGAAGAACGAATCC‑TGGc                             <  1:262445/251‑1 (MQ=255)                                                                                                                                                                                                            aaTAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAAGCGAATATGTT‑GGTTAGCAATGGAGAAAATA‑CCCATCGTGATTAACCAAACTCACGCAGGCGATGGG‑CCATTTTACGGAAGAACGAATCC‑TGGCTTtc                         >  1:101065/1‑251 (MQ=255)                                                                                                                                                                                                            aaTAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAAGCGAATATGTT‑GGTTAGCAATGGAGAAAATA‑CCCATCGTGATTAACCAAACTCACGCAGGCGATGGG‑CCATTTTACGGAAGAACGAATCC‑TGGCTTtc                         <  2:501652/251‑1 (MQ=255)                                                                                                                                                                                                             aTAGCGCCAGAATACGGTTCACATCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAAGCGAATATGTT‑GGTTAGCAATGGAGAAAATA‑ACCATCGTGATTAACCAAACTCACGCAGGCGGTGGG‑GCATTTTACGGAAGAACGAATCC‑TGGCTTtcg                        >  1:155161/1‑251 (MQ=255)                                                                                                                                                                                                               aGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAAGCGAATATGTT‑GGTTAGCAATGGAGAAAATA‑CCCATCGTGATTAACCAAACTCACGCAGGCGATGGG‑CCATTTTACGGAAGAACGAATCC‑TGGCTTtcgtc                      <  2:180839/251‑1 (MQ=255)                                                                                                                                                                                                               aGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAAGCGAATATGTT‑GGTTAGCAATGGAGAAAATA‑CCCATCGTGATTAACCAAACTCACGCAGGCGATGGG‑CCATTTTACGGAAGAACGAATCC‑TGGCTTtcgt                       <  1:459919/250‑1 (MQ=255)                                                                                                                                                                                                                      gAATATGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑CGTTTTACCGC‑AAAGCGAATATGTT‑GGTTAGCAATGGAGAAAATA‑CCCATCGGGGTTAACCAAACTCACGCAGGCGATGGG‑CCATTTTACGGAAGAAcacgatcggaagaacacacggctg                 >  1:368716/1‑227 (MQ=255)                                                                                                                                                                                                                      gAATATGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAAGCGAATATGTT‑GGTTAGCAATGGAGAAAATA‑CCCATCGTGATTAACCAAACTCACGCAGGCGATGGG‑CCATTTTACGGAAGAACg                                       <  2:368716/228‑1 (MQ=255)                                                                                                                                                                                                                        aTACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑CGTTTTACCGC‑AAAGCGAATATGTT‑TGTTAGCAATGGAGAAAATA‑CCCAACGTGATTTAACAAACTCCCGCCGGCGATGGG‑CCATTTTTCGGAAAAAAGAATCC‑TGGTCTTCGTCGCGATCTTg             >  2:439200/1‑251 (MQ=255)                                                                                                                                                                                                                            ggTTCACCCCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAAGCGAATATGTT‑GGTTAGCAATGGAGAAAATA‑CCCATCGTGATTAACCAAACTCACGCAGGCGATGGG‑CCATTTTACGgaaga                                          <  1:292931/219‑1 (MQ=255)                                                                                                                                                                                                                                  ccTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCCGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCCGTACAATGGCGGTCGCC‑AGTTTTACCGC‑CAAGCGCATATGTT‑GGGTAGCAATGGAGAAAATA‑CCCCTCGTGAATAAACAAACTCACCCAAGCGATGGG‑CCATTTTAAGGAAGAACGAATCCCTGG‑TTTCGTCGCGATCTTGttttcccgtg   >  1:257117/1‑244 (MQ=255)                                                                                                                                                                                                                                     cccGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAAGCGAATATGTT‑GGTTAGCAATGGAGAAAATA‑CCCATCGTGATTAACCAAACTCACGCAGGCGATGGG‑CCATTGTACGGAAGAACGAAGCC‑TGGCTTTCGTCGCGATCTGGTGTGCCAGTGa  <  2:284775/249‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               TGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGA‑GTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGC‑AAAGCGAATATGTT‑GGTTAGCAATGGAGAAAATA‑CCCATCGTGATTAACCAAACTCACGCAGGCGATGGG‑CCATTTTACGGAAGAACGAATCC‑TGGCTTTCGTCGCGATCTTGTTTGCCAGTGA  >  NC_000913/3387798‑3388273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑