Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,105,127	G→A	G232E (GGA→GAA)	mltC →	membrane‑bound lytic murein transglycosylase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,105,127	0	G	A	100.0%	204.8 / NA	60	G232E (GGA→GAA)	mltC	membrane‑bound lytic murein transglycosylase C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/30);  total (30/30)

CGCAAGCAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGAT  >  NC_000913/3104882‑3105371                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     cgcAAGCAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTGTGCGGTCAGCCGTTCCGATGCGCTGGAATTa                                                                                                                                                                                                                                                  >  2:135989/1‑250 (MQ=255)             cgcgGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCGCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTac                                                                                                                                                                                                                                     <  1:174800/251‑1 (MQ=255)                            gAAAAACCGTCTGAAGAGCCGCAGCACCGGGCGTCTTATCATCTACAGCGTCACCATTAACATGGTGCCCAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCATGCGTCACGGAAATATGGCGTTGATGAGGCGCTGATTTTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTTGTACAACATACTGCCgg                                                                                                                                                                                                                        <  2:233031/249‑1 (MQ=255)                              aaaaCCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGaaa                                                                                                                                                                                                                    <  2:449173/251‑1 (MQ=255)                              aaaaCCGTCTGAAGACCCGCAGCAACGGGCTTCGTATCAGCTACAGCGTCACCAATAACATGGTGCCGAACCACCGTGATAAACGGGCGCACAAAGATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGGGCCAATGCGCTTGAAGGAATGCAGTTGGTACAACATAcgggctggaaa                                                                                                                                                                                                                    <  2:38745/251‑11 (MQ=255)                               aaaCCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGaa                                                                                                                                                                                                                     >  1:443364/1‑249 (MQ=255)                                 aCCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGaa                                                                                                                                                                                                                     >  1:161184/1‑247 (MQ=255)                                  ccGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGAtg                                                                                                                                                                                                                <  1:515405/251‑1 (MQ=255)                                    gTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTCCAGCGTCACCATTAACATGGTGCCGAAACACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCAAGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACAGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGACGGGAAAgctg                                                                                                                                                                                                                >  2:138519/1‑246 (MQ=255)                                        gAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAAGGCAGGTGGTACAACATACTGCCGGGAAAGATgtgt                                                                                                                                                                                                             <  2:201189/248‑1 (MQ=255)                                         aaGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCg                                                                                                                                                                                                         >  2:148518/1‑251 (MQ=255)                                           gagCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCt                                                                                                                                                                                                       >  2:34161/1‑251 (MQ=255)                                               cGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGc                                                                                                                                                                                                    >  2:60324/1‑250 (MQ=255)                                                   gcaACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCGTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGATGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAgggg                                                                                                                                                                                               <  2:94011/251‑1 (MQ=255)                                                     aaCGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTacaa                                                                                                                                                                                                                                   >  2:339864/1‑213 (MQ=255)                                                     aaCGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTacaa                                                                                                                                                                                                                                   <  1:339864/213‑1 (MQ=255)                                                       cGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAAt                                                                                                                                                                                           <  1:254402/251‑1 (MQ=255)                                                        gggCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATc                                                                                                                                                                                          <  1:442107/251‑1 (MQ=255)                                                        gggCTGCGTATCATCTACAGCGTCACCATTAACATGATACCGAACCACCTTGATAAACGTACGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGCAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGACTTAATTCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATc                                                                                                                                                                                          >  2:91841/1‑251 (MQ=255)                                                              cGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGTCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGTTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCAc                                                                                                                                                                                    <  1:114357/251‑1 (MQ=255)                                                                    cccTACAGCGTCACCATTAACATGGTGCCGAACCCCCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGGCACGGAAATATGGCGTTGATGATTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTATCCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATTTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAg                                                                                                                                                                              <  2:173766/249‑1 (MQ=255)                                                                          aGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGGGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGc                                                                                                                                                                          >  2:125957/1‑249 (MQ=255)                                                                           gCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCACCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGt                                                                                                                                                                       <  1:389123/251‑1 (MQ=255)                                                                               cACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCt                                                                                                                                                                   >  2:448935/1‑251 (MQ=255)                                                                                aCCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCtt                                                                                                                                                                  <  1:81888/251‑1 (MQ=255)                                                                                     tAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCGCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGttt                                                                                                                                                              <  2:407475/250‑1 (MQ=255)                                                                                      aaCATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCCCGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTCTGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTCCAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGCAATCCGGCACGCCGAGc                                                                                                                                                                             >  1:327930/1‑234 (MQ=255)                                                                                      aaCAGGGTGCCGAACCACCTTGATAAACGGGCGCACAAATATCGCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGGTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGAGGCGCTGGAATTAATGCAGGGGGTACAACATACTGCCGGGAAAGAGGTGGGCCGCTCGCAGGGGAAATCCGGCACGCCGAGc                                                                                                                                                                             <  2:327930/234‑1 (MQ=255)                                                                                           ggTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGAGGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTGCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCGCGCCGAGCCGCAGTTTCTTGTTTGATCCt                                                                                                                                                        <  1:117074/250‑1 (MQ=255)                                                                                                 gAACCACCTTGCTACACGTGCGCCCCAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCAGAGCCGCAGTTTCTTGTTTGATCCTGCCAGcc                                                                                                                                                 >  2:226141/1‑250 (MQ=255)                                                                                                      accTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATAtt                                                                                                                                            >  1:375424/1‑251 (MQ=255)                                                                                                          tGATAAACGTTCGCACAAATAGCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAAGTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACTTACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAGATCCTGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGAt                                                                                                                                         <  1:459390/250‑1 (MQ=255)                                                                                                           gATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATa                                                                                                                                        <  1:205957/250‑1 (MQ=255)                                                                                                              aaaCGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCCGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCgg                                                                                                                                    >  2:231870/1‑251 (MQ=255)                                                                                                                  gTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGCAATATGGCGATGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCAGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCAcc                                                                                                                                >  2:83582/1‑251 (MQ=255)                                                                                                                    gcgcctaaagatCTCGGCATGGTCCGCCAGGCGTCTCGAAAATATGGCGTTGATGAGGCGCTGATTCTGGCTAGTATGCAGACCTAATCTTCTTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGTGACAACATACTGCTGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCTGCACGCCGAGCCTCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCTGCActgc                                                                                                                              <  2:384486/241‑4 (MQ=255)                                                                                                                       cacaAATATCACGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTa                                                                                                                           <  1:30710/251‑1 (MQ=255)                                                                                                                         caAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACcgcg                                                                                                                             <  2:167540/247‑1 (MQ=255)                                                                                                                         caAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACcgcg                                                                                                                             >  1:167540/1‑247 (MQ=255)                                                                                                                                ctcGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCgct                                                                                                                  >  1:266300/1‑249 (MQ=255)                                                                                                                                ctcGGCATGGTCCGCCAGGCGTAACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAAGCCGGCACGCCGAGCCGCAGTTTCTTGTTTGAGCCTGCCAGCCAGATTGATACCGGCCCCGCGTATCTGGCGAt                                                                                                                  >  2:13913/1‑251 (MQ=255)                                                                                                                                    gcagggcccgcacagcggcACGGAAATATGGAGTTGATGCGTCGCTGATTCAGGCAATTATGCAGACCGCATCTTCCTTTAACCCGAATGCGGTCAGCCGTTCCGCTGCGTTGGACTGAATGCAGGTGGTAAACCATACTGTCGGGAAAGATGTGATCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCCGCCCGCATTATTCCTTCCGGCACCTCGTATCTGGcactgctg                                                                                                              >  2:34852/19‑244 (MQ=255)                                                                                                                                       tGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAAc                                                                                                           >  1:60389/1‑251 (MQ=255)                                                                                                                                                    gTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTcg                                                                                              <  1:34161/251‑1 (MQ=255)                                                                                                                                                       aCGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTcggcg                                                                                           <  1:355213/251‑1 (MQ=255)                                                                                                                                                          gAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGa                                                                                         >  1:293430/1‑250 (MQ=255)                                                                                                                                                           aaaTATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTaa                                                                                                                                                                                                                                                 <  1:524418/97‑1 (MQ=255)                                                                                                                                                           aaaTATGGCGATGATGAGTCGCTGCTTCTGGCAATTATGCAGACAGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTaa                                                                                                                                                                                                                                                 >  2:524418/1‑97 (MQ=255)                                                                                                                                                                tGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGAt                                                                                   >  1:489578/1‑250 (MQ=255)                                                                                                                                                                          gaGTCGCTGATGCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATc                                                                        <  1:304278/251‑1 (MQ=255)                                                                                                                                                                                  gATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACa                                                                                                          >  1:146193/1‑209 (MQ=255)                                                                                                                                                                                  gATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACa                                                                                                          <  2:146193/209‑1 (MQ=255)                                                                                                                                                                                           aaTTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGtca                                                       <  1:226696/251‑1 (MQ=255)                                                                                                                                                                                                                cTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGgcggcg                                  >  1:516504/1‑251 (MQ=255)                                                                                                                                                                                                                     aCCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCacaggc                             >  1:373226/1‑251 (MQ=255)                                                                                                                                                                                                                          taggcgttCAGCCGTCCGGATGCGCTGGAATAAATGGAGGGGGACCAACATACGGCCGGGAAAGATGTTTTCCGCCCGCGGGGAAAAACCGGCGCGCCGACCCGCAGTTTCTTGTTTGACCCTCCCAGAAATATTGAAACCGGCCCCGCGTATCTGGGGAGGCTGAACAATGTTTACCCGGGCGAAATTGACACCCCAACGCCGGGGTGTTAGGCCGGCAGCACCGCCTATAACGGCGGCGCAGGCAGCg                         <  2:526950/243‑1 (MQ=255)                                                                                                                                                                                                                            tGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCCGGCCGCGtgc                      >  2:22827/1‑251 (MQ=255)                                                                                                                                                                                                                               ggTCAGCCGTTCCGCTGCGCTGGAATTCATGCAGGTGGTAAAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCCCGCCGAGACGCAGTTTCTTGTTTGCTCCTGCCAGCATTATTGATACCGACACCGCGTATCTGACGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCTTTATGCCGTCATCACCGCACATAAcggcgggccacgcagcgcgcctc                   >  2:322420/1‑234 (MQ=255)                                                                                                                                                                                                                                       gTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGGCttt           >  1:290983/1‑251 (MQ=255)                                                                                                                                                                                                                                                cgcTGGAATTAGTGAAGGGGGTACAACATACTGCGGGGAAAGATGTGTCCCGCTCGCGGGGAAAACCCGGCACGCCGAGCCGCAGTTTCTTGTTTGACCCTGCCAGCAATATTGATCCCGGCCCCGCGTATCGGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATACCCCAACACCGGGGCGTTATGCCGTCACCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGAt  <  1:135989/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     CGCAAGCAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGAT  >  NC_000913/3104882‑3105371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑