BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	365,306	G→A	R334C (CGC→TGC)	lacZ ←	beta‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	365,306	0	G	A	97.8%	294.1 / NA	90	R334C (CGC→TGC)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (45/43); total (46/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGC‑TGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGT  >  NC_000913/365061‑365545

atTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGaaa                                                                                                                                                                                                                                              <  2:443765/250‑1 (MQ=255)

  tGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAAcc                                                                                                                                                                                                                                            <  1:247720/250‑1 (MQ=255)

  tGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGATCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCATAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAAcc                                                                                                                                                                                                                                            <  2:182519/250‑1 (MQ=255)

     cTTCATCAACCACATACAGGACGTAGCGGTCGCACCGCGTGTACCACAGCGGCTGCTTCTGCTAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGCTGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCCGCAGCAG‑ACCATTTTCACTCCGCCTCTCGCGGAAACCgcc                                                                                                                                                                                                                                         >  2:322763/1‑248 (MQ=255)

          tCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGc                                                                                                                                                                                                                                    >  1:519707/1‑250 (MQ=255)

            caccacATACCAGCCGAAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAgg                                                                                                                                                                                                                                 >  2:59584/1‑251 (MQ=255)

            caccacATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACCTCGCAgg                                                                                                                                                                                                                                 >  2:238689/1‑251 (MQ=255)

                acaTACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTc                                                                                                                                                                                                                             >  2:51327/1‑251 (MQ=255)

                  aTACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGCTATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTg                                                                                                                                                                                                                           >  1:276737/1‑251 (MQ=255)

                    aCAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTGCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTTCTCATCCATGGCCTGACCGTGCAGAGGATGATGCTCTTGACGGTTAACGCCTCGAATCAGCAACGGCGTGCCGTGCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGAc                                                                                                                                                                                                                                         <  1:38044/235‑1 (MQ=255)

                    aCAGGCCGTAGAGGTCGCACAGCGTGTACCACCGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTACAGTTGTTCTGCTTCATCAGCAGGATCTCCTGCACCATCGTCTGCTCATCCATGACATGACCATGCAGAGGATGATGATCGTGACGGTTAACGCCTCGCATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCAACTCGCAGAAACCGAc                                                                                                                                                                                                                                         >  2:38044/1‑235 (MQ=255)

                     cAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACTGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCGCCTCGCAGAAACCGACATCGCAGGCTTCTGCtt                                                                                                                                                                                                                        <  2:277353/251‑1 (MQ=255)

                      aGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTc                                                                                                                                                                                                                       >  2:533549/1‑251 (MQ=255)

                          cGTAGCGGTCGCACAGCGTGTTCCACAGCGGATGGTTCGGAGAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCAGCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCCCAGGCTTCTGCTTCAATc                                                                                                                                                                                                                   <  2:249795/251‑1 (MQ=255)

                           gTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATCCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGCTATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGAAGCAGCAG‑ACCATTTTCAAACCGCACATCGCAGCAACCGACATCGCAGGCTTCTGCTTCAATc                                                                                                                                                                                                                   >  1:522863/1‑250 (MQ=255)

                                 gTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGc                                                                                                                                                                                                            <  1:380270/251‑1 (MQ=255)

                                    gCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTACAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATAGCAGGCTTCTGCTTCAATCAGCGTGCCGt                                                                                                                                                                                                         >  2:73265/1‑251 (MQ=255)

                                       caGCGTGTACCCCAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTcgg                                                                                                                                                                                                      <  2:150846/251‑1 (MQ=255)

                                         gCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTcggcg                                                                                                                                                                                                    <  2:366804/251‑1 (MQ=255)

                                         gCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTcggcg                                                                                                                                                                                                    <  1:429283/251‑1 (MQ=255)

                                            tgtACCAAAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGgtg                                                                                                                                                                                                 >  2:482347/1‑251 (MQ=255)

                                               acaacagaggctGGTACGGATAATGCGAACAGCGCACGGAGCTAAAGTTGTTCTTCTACATCAGCAAGATATCCTGCACCATCGTCTGATCATCCATGACCTGACCCTGCAGAGGATGATGCTCGTGACGGTTAACGACTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCACTCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGATTCAATCAGCGAGCCGTCGGCGgtgggc                                                                                                                                                                                              >  2:507233/12‑248 (MQ=255)

                                                ccACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGc                                                                                                                                                                                              >  1:43657/1‑250 (MQ=255)

                                                    aGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGACTTCTGCTTCAATCAGAGTGCCGTCGGCGGTGTGCAGtt                                                                                                                                                                                          >  1:451211/1‑250 (MQ=255)

                                                    aGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGAGGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTc                                                                                                                                                                                         <  2:391862/251‑1 (MQ=255)

                                                        gATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCGCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAacc                                                                                                                                                                                     <  2:80631/251‑1 (MQ=255)

                                                                  aTAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCCACCACCGCACgct                                                                                                                                                                           >  1:91522/1‑249 (MQ=255)

                                                                    aaTGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGAGCTCCTGCCCCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGAGGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCTCACCTCGCAGAAACCGACATCGCAGGCTTCTGCCTCAAGCAGCGTGCCGTCGGGTGTGTGCAGGTCATCCACCTCACGATag                                                                                                                                                                         <  2:344448/251‑1 (MQ=255)

                                                                       gCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCAGCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCATCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGGGCAGTTCAACCACCGCACGATAgaga                                                                                                                                                                      <  2:240414/251‑1 (MQ=255)

                                                                         gAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCACCCACCGCACGATAGAGAtt                                                                                                                                                                    >  1:287549/1‑251 (MQ=255)

                                                                                gcACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCTAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCAGCAACTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGAtt                                                                                                                                                             >  1:283543/1‑251 (MQ=255)

                                                                                gcACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGAAAGAGATTCGGGAtt                                                                                                                                                             >  2:252719/1‑251 (MQ=255)

                                                                                          aaaGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATGTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTcc                                                                                                                                                   <  2:3228/251‑1 (MQ=255)

                                                                                              ttgttCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCCGTTCAACCACCGCACGATAGAGATTCGGGaagagcggaagagcgtg                                                                                                                                               >  2:146356/1‑235 (MQ=255)

                                                                                              ttgttCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGa                                                                                                                                                               <  1:146356/235‑1 (MQ=255)

                                                                                                   cTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGATCTGCCCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑AACATTTTCAATAAGCACCTCGAAGAAACCGACATAGCAGGCTTCTGCTTCAATCAGCGAGCCGCAGGCGGGGAGCAGTTCAACCCCCGACCGATAGAGATTCGGGATTTTGGCGCTCCACAGTTTCg                                                                                                                                          >  2:195807/1‑251 (MQ=255)

                                                                                                    tGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCgg                                                                                                                                         >  1:199599/1‑251 (MQ=255)

                                                                                                    tGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCgg                                                                                                                                         >  1:51148/1‑251 (MQ=255)

                                                                                                     gCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTGTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGAGAGAGATTCGGAAGTTCGGCGCTCCACAGTTTCg                                                                                                                                          <  2:497341/249‑1 (MQ=255)

                                                                                                     gCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAATTTCggg                                                                                                                                        <  1:396191/251‑1 (MQ=255)

                                                                                                       ttCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGtt                                                                                                                                      >  1:13940/1‑251 (MQ=255)

                                                                                                          atcaGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTc                                                                                                                                   <  2:430775/251‑1 (MQ=255)

                                                                                                          atcaGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGCTTTTc                                                                                                                                   <  2:542744/251‑1 (MQ=255)

                                                                                                             agcagGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGAc                                                                                                                                >  1:325697/1‑251 (MQ=255)

                                                                                                                   atatCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAg                                                                                                                          <  2:51148/251‑1 (MQ=255)

                                                                                                                     atCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATGGAGATTCGGGATTTAGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGAc                                                                                                                        >  2:159037/1‑251 (MQ=255)

                                                                                                                     atCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGCCGTTCAGAc                                                                                                                        >  2:174093/1‑251 (MQ=255)

                                                                                                                     atCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGa                                                                                                                         <  1:40775/250‑1 (MQ=255)

                                                                                                                     atCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGAc                                                                                                                        >  2:272207/1‑251 (MQ=255)

                                                                                                                         tGCACCATCGTCTGCTCATCAATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGt                                                                                                                      >  1:443254/1‑249 (MQ=255)

                                                                                                                           cACCAGCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGCTCATCGTGACGCTTAACGCCTAGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCCTTTTCAATCAGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCAGTCGGCGGTGCACAGTTCAGCCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACgatacatcgtactg                                                                                                                  >  2:264925/1‑238 (MQ=255)

                                                                                                                              cATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGa                                                                                                               <  2:519707/251‑1 (MQ=255)

                                                                                                                                ccGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGAcg                                                                                                             <  2:525907/250‑1 (MQ=255)

                                                                                                                                 cGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTGTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGAcgc                                                                                                            <  1:43930/251‑1 (MQ=255)

                                                                                                                                  gTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGAcgcg                                                                                                           <  1:238689/251‑1 (MQ=255)

                                                                                                                                  gTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGGTTTCGCGGTTCAGACGTAGTGTGAcgcg                                                                                                           >  2:75050/1‑251 (MQ=255)

                                                                                                                                         cATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGGGTGCAGTTCAACCACCGCACGCTAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGc                                                                                                     >  2:18179/1‑250 (MQ=255)

                                                                                                                                          aTCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCAt                                                                                                   <  2:381652/251‑1 (MQ=255)

                                                                                                                                                  cctgaccATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAAccacca                                                                                           <  2:253582/251‑1 (MQ=255)

                                                                                                                                                         aTGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCAt                                                                                    >  1:414331/1‑251 (MQ=255)

                                                                                                                                                         aTGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCAt                                                                                    >  1:254681/1‑251 (MQ=255)

                                                                                                                                                           gCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATc                                                                                   <  2:503795/250‑1 (MQ=255)

                                                                                                                                                           gCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATc                                                                                   <  2:254681/250‑1 (MQ=255)

                                                                                                                                                              gagGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATa                                                                               <  1:533549/251‑1 (MQ=255)

                                                                                                                                                               agGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAaccacc                                                                                                                                                                                  <  1:470147/151‑1 (MQ=255)

                                                                                                                                                               agGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAaccacc                                                                                                                                                                                  >  2:470147/1‑151 (MQ=255)

                                                                                                                                                                          cGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACAGCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCAccgc                                                                     <  1:77972/249‑1 (MQ=255)

                                                                                                                                                                            tGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGAAGTTCAGACGTAGTGGGACGCGATCGGCATAACCACCACGCTCATCCATAATTTCCCCGCCGa                                                                  >  2:213910/1‑250 (MQ=255)

                                                                                                                                                                                ggTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCCCAGTTTCGGGTTTTCGCCGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGgc                                                             <  2:148302/251‑1 (MQ=255)

                                                                                                                                                                                 gTTAACGCCCCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAAGCCGCACCTCGCAGAACCGGACTACGCAGGCTTCTGCTTAAATCAGCGTGCCGTCGGGGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTCCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGTCGTATTGTGACGCGATGGGCATACCCACCACGCTCATCGATAGTTTCACCGCCGAAAGgcg                                                            <  2:246247/251‑1 (MQ=255)

                                                                                                                                                                                   tAACGCCTCGCATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGcgcg                                                          >  1:418156/1‑251 (MQ=255)

                                                                                                                                                                                        ccTCGATTCAGCAACGGCTTGCCTTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCCCCTCGCAGAACCCGACACCGCAGGCTTCTGCTTCAATCAGCGGGCCGCCGGCGGTGTGCAGTTCAACCACCGCACAATAGAGATTCGGGATTCCGGCGCTCCGCATTTTCGGGTTGTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCTTCGATAATTTCACCGCCGAAAGGCGCGGTGcc                                                     <  2:276737/251‑1 (MQ=255)

                                                                                                                                                                                        ccTCGAATCAGCACCGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAACCCGCCCCCCGCAGAAACCGACATCGCAGGCTCCTGCTTCAATCAGCTTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATGCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCAGCGATAATTTCACCGCCGAAAGGCGCGGTGcc                                                     <  1:456564/251‑1 (MQ=255)

                                                                                                                                                                                           cGAATCAGCAACGGCTTGCCGTTCAGCAGCAGAAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGCCATCGCAGGCTCCTGCTGCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCACCCACCGCACGATAGAGATTCGTGATTTCGGCGCCCCACAGTTTCGGGTTTGCGACGTGCAGACGTAGTTTGACGCGATCGGCATAACCCCCACGCTCACCGATAATTTCACCGCCGAAAGGCGCGGTGCCGC‑t                                                 <  1:239402/251‑1 (MQ=255)

                                                                                                                                                                                               tCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGATCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGAGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGC‑TGGCg                                             >  1:358945/1‑251 (MQ=255)

                                                                                                                                                                                                   cAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGC‑TGGCGACCt                                         >  1:512540/1‑251 (MQ=255)

                                                                                                                                                                                                    aaCGGCGTGCCGTCCAGCAGCAG‑AGTACCATTTGAAATCCGCCCCGCGCAGAAACCGACATCCCAGGCTTCTGCTTCAATCAGCGGCCCGTCGGCGGTGTGCAGTGCACCCACCGCAGGATAGAGATTCGGGATTGCTGCGCTCCACAGTTGCGGGTTTCCGACGTTCAGACGTGGTGTAACGGGATCGGCATACCCACCATGCTCTTCGATATTTTCTCCGCCGAAAGGCGCGGTGCCGC‑TGGCGACCTg                                        <  2:193081/251‑1 (MQ=255)

                                                                                                                                                                                                       gtcTTGCCGTTCAGCAACAGCAA‑CCCATTTTCAATCCGCACCTCGCAGAAACCGACTTCGCAGGCTTCTCCTTCAATCAGCGTGCCGCCGGCGGTGTGCAGTCCAACCCCCGCACGATAGAGATTCGGGATTTCGGCGCTCCACATTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGGGATCGGCATAACCACCCCGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGC‑TGGCGACCTGCGt                                     <  1:251251/249‑1 (MQ=255)

                                                                                                                                                                                                            gCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGGGTGCAGTTCAACCACCGCACGATAGAGATTTGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCCGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGCAAGGCGCGGGGCCGC‑TGGCGACCTGCGTTTCAc                                >  2:45117/1‑251 (MQ=255)

                                                                                                                                                                                                             ccGTTCAGCAACACCCG‑CCCATTTTCAATCCGCCCCCCGCAGACACCGACACCGCAGGCTTCTGCTCCAATCAGCGTGCGGTCGGCGGTGTGCAGTTCAACCACCGCAAGTTAGAGATGCGGGATTTCGGCGCTCAACAGTTCCGGGTTTGCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCCCCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGC‑TGGCGACCTGCGTTTCAcc                               <  1:440111/251‑1 (MQ=255)

                                                                                                                                                                                                                 tCAGCCGCAGCAG‑TCCATTTTAACCCCGGCCCTCGCGGAAGCCGCCACCCCAGGCTGCTGCGTCAATCGGCGGGCCTCCGGCGGTGTGCAGTTCAACCACCTCATGATAGAGATTCGGGATTTCGGCGCTCCACAGTGTCGGGTTTCCGCCGTTCAGACGGAGTGGGACGCGATCGGCATACCCACCACGCTCATCGATAAGTCCACCGCCGAAAGGCGCGGTGCCGC‑TGGCGACCTGCGTTTCccccgg                            <  2:46680/250‑3 (MQ=255)

                                                                                                                                                                                                                      agcagcag‑ACAATTTTCAATACGCCCCTAGCAGAAACCGACATCGCAGGCATCTGCTTCAATCAGCGAGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGGTTTAGGCGCTCCCAAGTTTCGGGTTTTCGACGTTCAGACGTAGGGGGTCGCGTTCGGCATAACCACCCCGCTCATCGATAATTTCACCGCCGAAAGTAGCCGTGCCGC‑TGGCGAACTGCGTTTCACCCTGcacgc                       >  2:472290/1‑246 (MQ=255)

                                                                                                                                                                                                                        cagcag‑ACCATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGC‑TGGCGACCTGCGTTTCACCCTGCCATaa                      >  1:393647/1‑249 (MQ=255)

                                                                                                                                                                                                                        cagcag‑ACCATTTTAAATCCCCCCCTCGCAGAAACCGACATCGCAGTCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGCCGTTCAGACGTAGTGGGACGCGATCGGCATACCCACCCCGCTCATCGATAATTCCACCGCCGAAAGGCGCGGTGCCGC‑TGGCGACCTGCGTTTCACCCTGCCATAAAg                    <  1:204798/251‑1 (MQ=255)

                                                                                                                                                                                                                                ccATTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGATTTc                                                                                                                                   >  1:397635/1‑134 (MQ=255)

                                                                                                                                                                                                                                ccATTTTCAATCCGCACCGCGCAGAAACCGACAGCGCAGGCTGCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTc                                                                                                                                   <  2:397635/134‑1 (MQ=255)

                                                                                                                                                                                                                                  aTTTTCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAGGGCCCGG‑GCCGCCTGCGGCCCTGCGTTTCCCCCGCCCATAAGGAAAcgttt            >  1:29372/1‑246 (MQ=255)

                                                                                                                                                                                                                                    tttCAATCCGCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGCGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGGTCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGC‑TGGCGACCTGCGTTTCACCCTGCCATCAAGAAACTGTTAcc         >  2:150731/1‑251 (MQ=255)

                                                                                                                                                                                                                                         aTCCGCACATCGCAGAAACCGACATCGCAGGCTTATGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCCACCACCGCACGATAGAGCTTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGCAGTTAAGACGTAGTGTGACGCGATCGGCATAACCACCACGATCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGC‑TGCCGAACTGCGGTTCACCCTGCCATACAGAAACTGTTACCCgt      >  2:479315/1‑249 (MQ=255)

                                                                                                                                                                                                                                             gCACCTCGCAGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGC‑TGGCGACCTGCGTTTCACCCTGCCCTAAAGAAACTGTTACCCgtaggt  >  2:28038/1‑249 (MQ=255)

ATTGGCTTCATCCACCACATACAGGCCGTAGCGGTCGCACAGCGTGTACCACAGCGGATGGTTCGGATAATGCGAACAGCGCACGGCGTTAAAGTTGTTCTGCTTCATCAGCAGGATATCCTGCACCATCGTCTGCTCATCCATGACCTGACCATGCAGAGGATGATGCTCGTGACGGTTAACGCCTCGAATCAGCAACGGCTTGCCGTTCAGCAGCAGCAG‑ACCATTTTCAATCCGCACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGC‑TGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCCGTAGGT  >  NC_000913/365061‑365545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`