Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,880,635	T→C	L138L (TTA→TTG)	casD ←	type I‑E CRISPR system Cascade subunit CasD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,880,635	0	T	C	100.0%	153.8 / NA	41	L138L (TTA→TTG)	casD	type I‑E CRISPR system Cascade subunit CasD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/15);  total (26/15)

TACCTCCTTTAATCACATACCATTCTCGGGAAGCAAATTGTCGAGGCAAGGTGATCATCGGTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATCACGCAACCCCGTTACAGACACACGACGATCGTCAAGAATGAGTTCATCGCAGCGCACTGCAAATTGCACACTCTCTGATA  >  NC_000913/2880391‑2880879                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     tACCTCCTTTAATCACATACCATTCTCGGGAAGCAAATTGTCGAGGCAAGGTGATCATCGGTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACt                                                                                                                                                                                                                                                >  2:494625/1‑251 (MQ=255)        tttAATCACATACCATTCTCGGGAAGCAAATTGTCGAGGCAAGGTGATCATCGGTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGCGTATACCGAGGCTTCAATACTGCttttt                                                                                                                                                                                                                                         >  1:246299/1‑251 (MQ=255)                   aCCATTCTCGGGAAGCAAATTGTCGAGGCAAGGTGATCATCGGTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGACCCAGGTAAGGGGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTg                                                                                                                                                                                                                                >  2:403375/1‑249 (MQ=255)                           cGGGAAGCAAATTGTCGAGGCAAGGTGATCATGGGTTCGTCGCGCTCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCCCTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCATTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCa                                                                                                                                                                                                                       <  2:8517/250‑1 (MQ=255)                           cGGGAAGCAAATTGTCGAGGCAAGGTGATCATCGGTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAAc                                                                                                                                                                                                                         <  2:21366/248‑1 (MQ=255)                                   aaaTTGTCGAGGCAAGGTGATCATCGGTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAAGCATCGTTGCAt                                                                                                                                                                                                              <  1:139730/251‑1 (MQ=255)                                                 aGGTGATCATCGGTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAAcc                                                                                                                                                                                                  >  1:30363/1‑249 (MQ=255)                                                      atcatcGGTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGc                                                                                                                                                                                           >  1:434332/1‑251 (MQ=255)                                                        catcGGTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGCGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCg                                                                                                                                                                                          >  2:126608/1‑250 (MQ=255)                                                        catcGGTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCg                                                                                                                                                                                          <  1:393590/250‑1 (MQ=255)                                                         atcGGTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGAc                                                                                                                                                                                        >  2:426599/1‑251 (MQ=255)                                                                    gcgcgcCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAaggagg                                                                                                                                                                             <  1:239005/251‑1 (MQ=255)                                                                      gcgcCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGGATACCGAGGCTTCAATACTGCGTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCa                                                                                                                                                                           <  1:481529/251‑1 (MQ=255)                                                                            tAAATTTTAACTGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGACAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGCGTATACCGAGGCTTCACTACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACAt                                                                                                                                                                     >  1:199638/1‑251 (MQ=255)                                                                                        gatgCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCgcgc                                                                                                                                                         >  2:308854/1‑251 (MQ=255)                                                                                                gTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTg                                                                                                                                                 <  1:549635/251‑1 (MQ=255)                                                                                                                            cgccCACGGGCTCCTAATTTAATAGCGCCTTCTGCGGATCCGATGCCTGAAATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGAGTTAACCAGAGAGCGACGGTAAAGGCGGCATCACATAAATATTCGCGCCATATTTGACTCGTTTCATGACTTTTCAAACCACgg                                                                                                                     >  1:420569/1‑251 (MQ=255)                                                                                                                             gccAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGt                                                                                                                    <  1:7456/251‑1 (MQ=255)                                                                                                                                       tCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTcg                                                                                                          >  1:363212/1‑251 (MQ=255)                                                                                                                                        cATAATTTAATAGCGCCTTTAGATGATCCGATGCCTGACAAGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGTTAAGGTGTATACCGAGGCGTCAATACTGCTTTGTCAAGCTCTGAAAAAACCATCGTTGCATGGTGTGTGAACCAGAGAGCTACGGTAGAGGAGGCACCACATAAATATACGCGCCATGTTTGAATCGTTTCATGACTTTTCCATCCACGGTAGTCTtctc                                                                                                           <  2:277356/249‑1 (MQ=255)                                                                                                                                                      gcCTTCTGAAGATCCGATGCCTGAAATGTCCCCAAAAACAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGCCTGt                                                                                           >  2:184686/1‑251 (MQ=255)                                                                                                                                                       ccTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCGAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAACGACTGt                                                                                           >  1:216017/1‑250 (MQ=255)                                                                                                                                                            tGAGGCTCCGATGCCTGACCTGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGACTGTATGGt                                                                                      >  2:85281/1‑250 (MQ=255)                                                                                                                                                                gATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATc                                                                                  >  1:254865/1‑250 (MQ=255)                                                                                                                                                                   ccGATGACTGACATGTCACCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTACTCTTCTCGCGCTCCAAGGACTGTATGGTAATCACGc                                                                              >  1:384305/1‑251 (MQ=255)                                                                                                                                                                          cTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGTCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACt                                                                                                                                                                                                                                                <  1:226399/82‑1 (MQ=255)                                                                                                                                                                          cTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACt                                                                                                                                                                                                                                                >  2:226399/1‑82 (MQ=255)                                                                                                                                                                           tGACATGTCCCCAAAAAAAGCGGGTGAGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGAGTATACCGCGGATTCACTACTGCTATTTCAAGTTCTGAGATAACCATCGTTTCCTGGGGTGTTCACCAGAGAGAGACGGTAAAGGCGGCATCACATAAATATTCGCGCCATGTTTGCATCGTTTCATAACTTTTCAAACCCCGGCAATCTTCTCGCGCTCCAAGGACTGTATGGTACTCACGCacacccgt                                                                      >  1:47410/1‑244 (MQ=255)                                                                                                                                                                            gACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATCACGCACCCCCGt                                                                      <  2:262466/250‑1 (MQ=255)                                                                                                                                                                            gACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATCACGCAACCCCGt                                                                      <  1:85281/250‑1 (MQ=255)                                                                                                                                                                                                gggTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAaggagg                                                                                                                                                                             >  1:351779/1‑127 (MQ=255)                                                                                                                                                                                                gggTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAaggagg                                                                                                                                                                             <  2:351779/127‑1 (MQ=255)                                                                                                                                                                                                gggTGAGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATCACGCAACCCCGTTACAGACACACGACGATCGTc                                                 >  1:119177/1‑251 (MQ=255)                                                                                                                                                                                                          gTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATCACGCAACCCCGTTACAGACACACGACGATCGTCAAGAATGAGt                                       >  1:479931/1‑251 (MQ=255)                                                                                                                                                                                                                aaCTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATCACGCAACCCCGTTACAGACACACGACGATCGTCAAGAATGAGTTCATCg                                 >  2:454776/1‑251 (MQ=255)                                                                                                                                                                                                                           cccAGGTAAGGTGTATACCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATCACGCAACCCCGTTACAGACACACGACGATCGTCAAGAATGAGTTCATCGCAGCGCACTGc                      >  1:323803/1‑251 (MQ=255)                                                                                                                                                                                                                                        tataCCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCGAGGACTGTATGGTAATCACGCAACCCCGTTACAGACACACGACGATCGTCAAGAATGAGTTCATCGCAGCGCACTGCAAATTGCACActc         >  1:166807/1‑251 (MQ=255)                                                                                                                                                                                                                                           aCCGAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATCACGCAACCCCGTTACAGACACACGACGATCGTCAAGAATGAGTTCATCGCAGCGCACTGCAAATTGCACActctct      >  2:455829/1‑251 (MQ=255)                                                                                                                                                                                                                                              gAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCGAGGACTGTATGGTAATCACGCAACCCCGTTACAGACACACGACGATCGTCAAGAATGAGTTCATCGCAGCGCACTGCAAATTGCACACTCTCTGAt   <  2:166807/251‑1 (MQ=255)                                                                                                                                                                                                                                              gAGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTATCGCGCTCCAAGGACTGTATGGTAATCACGCAACCCCGTTACAGACACACGACGATCGTCAAGAATGAGTTCATCGCAGCGCACTGCACATTGCACACTCTCTGAt   >  1:53691/1‑251 (MQ=255)                                                                                                                                                                                                                                               aGGCTTCAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGTGTTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATCACGCAACCCCGTTACAGACACACGACGAGCGTCAAGAAGGAGTTCATCTCAGCGCACTGCAAATTGcactcgcgctgata  <  2:77023/251‑12 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     TACCTCCTTTAATCACATACCATTCTCGGGAAGCAAATTGTCGAGGCAAGGTGATCATCGGTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATCACGCAACCCCGTTACAGACACACGACGATCGTCAAGAATGAGTTCATCGCAGCGCACTGCAAATTGCACACTCTCTGATA  >  NC_000913/2880391‑2880879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑