Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,745,600	A→G	Y107H (TAC→CAC)	rimM ←	ribosome maturation factor RimM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,745,600	0	A	G	100.0%	238.2 / NA	62	Y107H (TAC→CAC)	rimM	ribosome maturation factor RimM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/32);  total (30/32)

CCGGTGGTTTAAAAACCAGGATCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTG  >  NC_000913/2745362‑2745832                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                         ccGGTGGTTTAAAAACCAGGATCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCct                                                                                                                                                                                                                               <  1:460050/250‑1 (MQ=255)  cGGTGGTTTAAAAACCAGGATCCAAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGCTAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGATTCCATCCTATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCctct                                                                                                                                                                                                                             >  2:219517/1‑251 (MQ=255)          taaaagcaaggttaccAATCTACTTCGATTGCACGAGTCGTGAGATAGACTTTCTTGATAACCTGCCCCTCGAGGAACGGTACGAGACGTTCCTTGATCCCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAGCGTCATTAGATACGGTTTCCATCGTATCGACAACTTTACCGAGATCGTAGCATTCAGTGGTTACTACCCTGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAgatg                                                                                                                                                                                                                     >  2:74825/15‑248 (MQ=255)            aaaaCCAGGATCCCAATCTACTACGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCGTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGTTTTCCATCATATCGACGTCTTTACCGAGATCGTAGCCTTCAGTGGTTACGGCCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAg                                                                                                                                                                                                                   <  1:350822/251‑1 (MQ=255)             aaaCCAGGATCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCCTCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCCTCATATCGACGACTTTACCGCGCTCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTgcgg                                                                                                                                                                                                                  >  1:91267/1‑248 (MQ=255)             aaaCCAGGATCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAgg                                                                                                                                                                                                                  >  1:382186/1‑251 (MQ=255)                 cAGGATCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCATGCTGAGGCAGc                                                                                                                                                                                                              >  1:518260/1‑251 (MQ=255)                 cAGGATCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTGTCTTGATAACCGGCCCAGCGAGGAACGTTACGAGACGTTCCTTGATACCAAACTCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCAGCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTGCTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGc                                                                                                                                                                                                              <  1:66611/251‑1 (MQ=255)                     aTCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCg                                                                                                                                                                                                          <  1:453593/251‑1 (MQ=255)                         cAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGa                                                                                                                                                                                                      <  1:307340/251‑1 (MQ=255)                         cAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGa                                                                                                                                                                                                      <  1:132038/251‑1 (MQ=255)                          aaTCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGa                                                                                                                                                                                                      >  1:25614/1‑250 (MQ=255)                                          aCGAGTAGTGAGATCGACTTTCTTGATAAACTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAAAGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATAGTAGCCTTCAGTGGTTACTACCTGGAAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGCATCCACGACAATTTcac                                                                                                                                                                                     >  2:34546/1‑251 (MQ=255)                                                    agaTCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTcag                                                                                                                                                                           <  1:155915/251‑1 (MQ=255)                                                          aCTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGtt                                                                                                                                                                     <  1:431751/251‑1 (MQ=255)                                                                 tGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATTCCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGGTCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCATCCCATCAGGGCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCTCGACAATTTCACAATTCGTCAGCAGGTTcgccg                                                                                                                                                                <  2:169350/249‑1 (MQ=255)                                                                 tGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCGTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCGGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTcgcc                                                                                                                                                                 <  2:250351/248‑1 (MQ=255)                                                                     aaCCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGAGACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTATATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCGTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCAc                                                                                                                                                          <  2:224471/251‑1 (MQ=255)                                                                         tGCCCCTCGAGGAACGGAACGCGACGTTCATTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATc                                                                                                                                                      >  1:432416/1‑251 (MQ=255)                                                                                           aCGAGTCGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAAAACGTCATTAGATCCGGTTTCCATCATATCGACGTCTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGCCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCtt                                                                                                                                    <  1:6748/251‑1 (MQ=255)                                                                                           aCGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCtt                                                                                                                                    <  2:492698/251‑1 (MQ=255)                                                                                               gaCGTTCCTTGAGACCAAACGCAGCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTATCCTTCAGTGGTTACTACCTGGCAGCCCAGCAGGGCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGGCAGCAGGTTCGCCGCATCACGATCGTCACCGCCTTTCAGCTtgatg                                                                                                                                <  2:525823/251‑1 (MQ=255)                                                                                                        tGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTc                                                                                                                        <  2:271645/250‑1 (MQ=255)                                                                                                        tGATACCAAAAGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGCCGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTcc                                                                                                                       >  2:235076/1‑251 (MQ=255)                                                                                                          aTACCAACCGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTTCCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTg                                                                                                                     <  1:390891/251‑1 (MQ=255)                                                                                                          aTACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCa                                                                                                                                                  >  1:350067/1‑222 (MQ=255)                                                                                                          aTACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCa                                                                                                                                                  <  2:350067/222‑1 (MQ=255)                                                                                                           tACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTCGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTg                                                                                                                     >  1:472324/1‑250 (MQ=255)                                                                                                               aaaCGCATCTTTCAGGTTTGCCGTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATtgt                                                                                                                <  2:382186/251‑1 (MQ=255)                                                                                                                         ttCAGGTTTGCCTTAATGACGAGAACGTCATGAGATCCGGTTTCCTTCATATCGACGACTGTACCGAGATCGTAGCCTTCATTTGTTACTCCCTGGCAGCCCCTCTGGTCTTTCCATTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGAGGAATCCACGACAATTTCACATTGCTTCAGCAGGTGCGCCGCAGCACGTTCGTCATCGCCTGTCATCATTATGATCATGTCCTGATTATGGTGCttcc                                                                                                       <  2:513670/250‑1 (MQ=255)                                                                                                                           cAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATtg                                                                                                                 <  1:214475/238‑1 (MQ=255)                                                                                                                           cAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATtg                                                                                                                 >  2:214475/1‑238 (MQ=255)                                                                                                                           cAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCttccagc                                                                                                    >  1:295909/1‑251 (MQ=255)                                                                                                                            aGGTTTGCCGTAATGACGAGAACGTCATTAGCACCGGTTTCCATCATATCGACGACTTTCCCGAGCTCGTCGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTTCCCCTCTTCAAGCTGAGGCAGCTTCGATGAATCCCCGACAATTTCACAATACGTCAGCAGGTTCTCCGCAGCACGATCGTCAACGCCTTTCTGCTTGATGAGCATGTCCTGATTATGGTGCttccaggt                                                                                                   <  2:461773/251‑3 (MQ=255)                                                                                                                             ggTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCttccagctt                                                                                                  >  1:71387/1‑251 (MQ=255)                                                                                                                                ttGCCTTACTGACGAGACCGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCttccagctttcc                                                                                               >  2:269763/1‑251 (MQ=255)                                                                                                                                        aTGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAAt                                                                                                                                                                                                    <  2:12323/142‑1 (MQ=255)                                                                                                                                        aTGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAAt                                                                                                                                                                                                    >  1:12323/1‑142 (MQ=255)                                                                                                                                          gACGAGCACGTCATTAGATCCGGTTTCCATCATATCGCCGACTTTCCCGAGATCGTAGCCTTCAGCAGATCCTACCTGGCAGCCCATCCGGTCTTTCCAGTGGTAGTCCCCCTCTTAACGCTGAGGCAGCTGCGCTGACTCCACGACAATTTCACAGTTCATCAGCAGGTTCGCCGCATCCCGATCGTCAACGCCTTTCAGCTTGATGATACTGTCATGATTGTGGTGCTTCCAGCTTTCCAGCTGGACtt                                                                                     >  1:247606/1‑251 (MQ=255)                                                                                                                                           aCGAGAACGTCATTAGATCCGGATTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTACCCCTCTTCAAGCTGCGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGCTGATCATGTCCTGATTGTGGAGCTTCCAGCTTTCCAACTGGACTtg                                                                                    >  2:165089/1‑251 (MQ=255)                                                                                                                                               gaACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTtgctgc                                                                                >  1:395098/1‑251 (MQ=255)                                                                                                                                                aaCGTCATTAGATCCGGTTTCCATCATCTCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCCAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGcc                                                                               >  2:48623/1‑251 (MQ=255)                                                                                                                                                    tCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTg                                                                           >  1:474900/1‑251 (MQ=255)                                                                                                                                                             cctgTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCATCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACt                                                                                      <  1:514285/228‑1 (MQ=255)                                                                                                                                                             ccGGTTTCCATCATATCGGCGACTTTACCGAGACCGTAGCCTGCAGTGGTTACTCCCTGGCAGCCCGGCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCCGCGATGATTCCACGACAATATCACAATTCGTCATCAGGGTCGCCGCATCTCGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCTCTGACCCGCCtt                                                                  <  1:542154/251‑1 (MQ=255)                                                                                                                                                             ccGGTCTCCATCATATCGCCGACTTTCCCGAGATCGTCGCCTTCAGTGGTTACTCCCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCCCGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTggcctagctcggacgcgcgtcgtgt                                                                  >  2:514285/1‑228 (MQ=255)                                                                                                                                                                 tttCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTgg                                                              >  1:196166/1‑251 (MQ=255)                                                                                                                                                                        catATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAAc                                                        <  1:516430/250‑1 (MQ=255)                                                                                                                                                                           atCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAg                                                     <  2:132994/250‑1 (MQ=255)                                                                                                                                                                           atCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTGCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAgg                                                    <  1:87905/251‑1 (MQ=255)                                                                                                                                                                                cgacTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCACCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTg                                                >  2:543062/1‑250 (MQ=255)                                                                                                                                                                                cgacTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAAACCATCAGGTCTTTCCCGTGGAAGTCCCCCTCTTCAAGCTGATGCAGCTGCGCTGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCCACGCCTTTCAGCTTGATGAGCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTg                                                >  2:417941/1‑250 (MQ=255)                                                                                                                                                                                       aCCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCACGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACCATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTATGGATAACCCAGGGCTGGTAGTCa                                         >  2:86598/1‑250 (MQ=255)                                                                                                                                                                                           agaTCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCaaacat                                    >  1:333939/1‑248 (MQ=255)                                                                                                                                                                                            gaTCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACACTTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATg                                   >  1:522141/1‑251 (MQ=255)                                                                                                                                                                                               cGTAGCCTTCAGTGGTTACTACCTGGCAGCCCAGCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATGCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTGCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCt                                 <  2:489670/250‑1 (MQ=255)                                                                                                                                                                                                         aGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCtt                      >  1:12965/1‑251 (MQ=255)                                                                                                                                                                                                                   acCTGGCAGCCCATCAGGTCTTTCCACTGGTAGTCCCCCTCTCCAAGCTGAGGCAGCTGCCATGAATCCACGACAACTTCACAATCCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTTTGGATAAACCAGTGCTGATAGTCAAAAATGCTTTCCGCGTCTTCCGTGgaag             <  2:25614/250‑1 (MQ=255)                                                                                                                                                                                                                     cTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGgaagaa           >  2:228528/1‑250 (MQ=255)                                                                                                                                                                                                                       ggCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGaaa          <  2:395098/249‑1 (MQ=255)                                                                                                                                                                                                                       ggCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGAGGAATCCACGACAATATCACATTTCGTCAGCAGGTTCGCCGCATCACTATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGAGTGTGGTGCTTCCATCTTTCCATCTGGACTTGCTTCCACTGTCCCGCCTTCTTGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGATGGAAGaaaa         <  2:530168/250‑1 (MQ=255)                                                                                                                                                                                                                             ccATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTg  <  1:67322/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                         CCGGTGGTTTAAAAACCAGGATCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTG  >  NC_000913/2745362‑2745832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑