Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,570,269 G→A P23P (CCC→CCT)  eutJ ← putative ethanolamine utilization chaperonin EutJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,570,2690GA95.0% 354.9 / NA 120P23P (CCC→CCT) eutJputative ethanolamine utilization chaperonin EutJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (63/51);  minor base C (6/0);  total (69/51)
Fisher's exact test for biased strand distribution p-value = 3.77e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

CCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCC‑GTCGCGAACCACAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGAAAATG  >  NC_000913/2570025‑2570510
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  
ccggaccgggaggaAAGGAAGGCGCCGCATGGAAAAAACGACGACAGAATTGCTGCTGGGGCGGGTCGAGAGGGCGACGAACAATGGTGACGGCGCCGAAGAAACCCCAGACGATGCC‑GGCGCGACCCACAT‑CGGCCCAGGCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTc                                                                                                                                                                                                                                              <  2:418301/243‑1 (MQ=255)
ccGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCC‑GTCGCGAACCACAT‑CGGCCCAGTCGAGG‑CATAACGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCCCCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTc                                                                                                                                                                                                                                              >  2:289968/1‑251 (MQ=255)
         gaggaAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACACTGGTGACAGCGCCGAAGAAATCCCAGACGATGCC‑GTCGCGAACCACAT‑CGGCCCAGTCGCGG‑CATACCGCCACTGGCTGAACGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTacac                                                                                                                                                                                                                                     >  1:251750/1‑251 (MQ=255)
         gaggaAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGCAGAAATCCCAGACGATGCC‑GTCGCGAACCACAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGGGCCCAGATCCACCCCCAGCCACAGCAGAGATTCCGTCGCGGCAGACGTCTGGTTaccc                                                                                                                                                                                                                                     >  1:310689/1‑249 (MQ=255)
           ggaAATGAAGTCGCCGCAGGGCTAAAACGACGACCGAATTGCGGCTCGGGCGTGTCGAGAGGGAGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCC‑GTCGCGAACCACGG‑CGGCCCAGTCTAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑g                                                                                                                                                                                                                                  <  2:199382/251‑1 (MQ=255)
            gaAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCC‑GTCGCGAACCACAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGACGGCGTCTGGTTacaccgc                                                                                                                                                                                                                                  >  2:450174/1‑248 (MQ=255)
             aaaTGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCC‑GTCGCGAACCACAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCCGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCg                                                                                                                                                                                                                                >  2:504082/1‑251 (MQ=255)
                tGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACTAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCC‑GTCGCGAACCACAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATAGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGCTTACACA‑GCGtcg                                                                                                                                                                                                                             >  1:450573/1‑249 (MQ=255)
                ggaagtggccgaatgtatAAAAGGACGACCGAGTTGCGGGTCGAGCGTTTCAAGAGGTCGACGAACAATGGTAACAGGGCCGAAGAAGCCCCAGACGAGGCC‑GTCTCGAGCCAGCT‑CTGCCCATTCGGGG‑CATACCGCCACTGGCTGACCGTCGCGGGCGACAACCATCGACACCACATCGCAGGTGCCCAGAGCCACCCCCAGCCGCAGCGGAGATTCCGTCGAGGCAGGCGTCTGGTTACACA‑GCGTTg                                                                                                                                                                                                                             <  1:35760/234‑1 (MQ=255)
                  aaGTCGCCGCATGGCTAAAACGACGACCGAATGGCTGCTCGAGCGTGTCGAGATGGCGACGAACAAGGGGGACAGCGCCGAAGAAATCCCAGACGATGCC‑GTCGCGAACCACAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTgcc                                                                                                                                                                                                                           <  1:16834/251‑1 (MQ=255)
                             tGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCC‑GTCGCGAACCACAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGc                                                                                                                                                                                                                  >  1:9298/1‑249 (MQ=255)
                                    aaCGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCC‑GTCGCGAACCACAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATAGCAGGGGCCCAGATCCACCCCCAGCCAAAGCGGAGATTCCGTCGCGGCAGGCGTCTGTGTACACA‑GCGATGCCCCCGTTTGCCGACGTggg                                                                                                                                                                                                         >  2:308645/1‑251 (MQ=255)
                                      cgacgccgaaatcgcgtctCGGGCGTGTCGAGATGGCGACAAACAATGGTGACCGCGCCGAAGAATTCCCAGACGATGCC‑GCCGCAAACCACAG‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGt                                                                                                                                                                                                       <  2:300891/234‑1 (MQ=255)
                                              aaatggctccccGAGGGTGTCGAGATGGCGACAAACAATGGTGACAGCCCCAAAGAATCCCCAGACGATGCC‑GTCGCAACCCCCAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCAtt                                                                                                                                                                                               <  1:23999/240‑1 (MQ=255)
                                                  ggtggctcaggcgggcggggaTGGCGACGAACAAGGGGGACAGCGCCAAAAAAACCCCAGACGGTGCC‑GTCCCGACCCACCC‑CGGCCAATCCGAGG‑CAACCCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTtc                                                                                                                                                                                           <  2:276686/232‑1 (MQ=255)
                                                    ctgctCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCC‑GTCGCGAACCACAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTTCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTtcgt                                                                                                                                                                                         >  1:3587/1‑251 (MQ=255)
                                                     tgctCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCC‑GTCGCGAACCACAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACAATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCCTTGTtcgtc                                                                                                                                                                                        >  2:418436/1‑251 (MQ=255)
                                                     cgctcgggcggggcGAGATGGGGACGAACAATGGTGACGGCGCCGAAGAAACCCCAGACGATGCC‑GTCGCGAACCACAT‑CGGCCCAGTCGGGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTtcgtc                                                                                                                                                                                        <  2:360400/238‑1 (MQ=255)
                                                      gctCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCC‑GTCGCGAACCACAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTtcgtcg                                                                                                                                                                                       >  1:129246/1‑251 (MQ=255)
                                                         cGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCC‑GTCGCGAACCCCAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGTTGAGCCAGTGTTcgtcgt                                                                                                                                                                                      <  1:427261/249‑1 (MQ=255)
                                                          gAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCC‑GTCGCGAACCACAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGGCTGGTTACCCA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGGgcg                                                                                                                                                                                   >  2:347004/1‑251 (MQ=255)
                                                           aGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCC‑GTCGCGAACCACAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCCGACGTGGGGAGAGCCATTGTTAGTCGTgcgc                                                                                                                                                                                  >  2:399852/1‑251 (MQ=255)
                                                                gtcaagaTGGCTACAAAAAAGGGTGACAGCGCCGAAAAAATCCCAGACTACGCC‑GTCGCGACCCACGT‑CGCCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTGAGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCt                                                                                                                                                                             <  1:196432/247‑1 (MQ=255)
                                                                gtCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAACCCCAGACGATGCC‑GTCGCGAACCCCAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATc                                                                                                                                                                              <  1:31809/250‑1 (MQ=255)
                                                                  cGAGAGGGCGACGAACAAGTGTGACAGCGCCAAAGAAATCCCAGACGATGCC‑GTCGCGAACCACAG‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGt                                                                                                                                                                           <  1:275336/251‑1 (MQ=255)
                                                                    ggaTGGCGACGAACAAGGGTGACAGCGCGGAAGAAATCCAAAACGAGGCC‑GTCGCAAACCCCAC‑CGGCCCAGCAGGGG‑CTTACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GAGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTa                                                                                                                                                                         <  1:11048/250‑1 (MQ=255)
                                                                     gaTGGCGAAGAACAAGGGTGACAGCGCGTAAGAAACCCCAGACGATGCC‑GTCGGCAACC‑CATGCGGCACAGTTGAGGACGT‑CCGCCACGGGCTGACCGTCGCGGGCGACAACCATCGACACCACATCGCAGGTGCCCAGAGCCACCCTCAGCCACAGCGGAGATTCCGTTGCGGCAGGCGTCTGGTTACACA‑GCGTGGCCGTCGTTTGCAGACGTGGTGTGAGCCATTGTTCGTCGTGCGCCCTCTGTTAc                                                                                                                                                                        <  2:506611/251‑1 (MQ=255)
                                                                        ggCGATGAACAAGGGTGACAGCGCCCAAGATATCCCAGACG‑TGCCCGTCGCGAACCACAC‑CGGCCCATTCGAGT‑ATTACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTGGCTGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTcc                                                                                                                                                                     <  1:331518/251‑1 (MQ=255)
                                                                            acgaACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCC‑GTCGCGAACCACAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTa                                                                                                                                                                  >  1:102348/1‑250 (MQ=255)
                                                                             cgaACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCC‑GTCGCGAACCACAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTTCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGGGCGCCATCTGTTACTCCTTaa                                                                                                                                                                 >  2:305096/1‑250 (MQ=255)
                                                                              gaACAATGGTGACAGCGCCGAAGAACTCCCAGACGCTGCC‑GTCGCGACCCACAT‑CGGCCCCGTCGAGG‑CCTACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGCTGCCGCCGTTTGCCGACGTGGGGTGAGCCATTGTTCGCCTTGCGCCATCGGTTACTCCTTAAAc                                                                                                                                                               >  2:196517/1‑251 (MQ=255)
                                                                              gaACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCC‑GTCGCGAACCACAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGGGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAAc                                                                                                                                                               >  2:101553/1‑251 (MQ=255)
                                                                              gaACAATGGTGACAGCGCCGAAGAAACCCCAGACGATGCC‑GTCGCGACCCACAC‑CGGCCCAGTCGATG‑CATACCGCCACTGGCTGACCGTCGCGGGCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAAc                                                                                                                                                               <  1:194946/251‑1 (MQ=255)
                                                                                  aaTGGTGACAGCGCAGAAGCAATCCCAAACGATGCC‑GTCGCGCACCACAT‑CGGCCCAGCCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACAATCGACCACACATCGCAGGAGCCCAGATCCACCCCCAGCAACAGCGGAGATTCAGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGCTTGCAGACGTGGGGTGACCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATg                                                                                                                                                           >  2:225837/1‑251 (MQ=255)
                                                                                   gtGGTTACAGCGCCGAAGAAACCCAAGGCGATGCC‑GGCGGTACCCACAT‑CGCCCAAGTCGAGG‑AATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGTCGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGc                                                                                                                                                          <  2:534566/250‑1 (MQ=255)
                                                                                    tGGTGACAGCGCCGAAGAAATCCCAGACGATGCC‑GTCGCGAACCACAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCg                                                                                                                                                         >  1:209721/1‑251 (MQ=255)
                                                                                       ggACAGCCCCAAAAAAATCCCAGACGGTGCC‑GCCGCGAACCACAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCTGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGaaa                                                                                                                                                      <  1:320249/250‑1 (MQ=255)
                                                                                          cAGCGACGAAGAAATCCCCGACGATGCC‑GTCACGAACCACAT‑CAGCCCAGCCGAGA‑CATACCGCCACTGGATGACCGTCGCGACCGACAACCATCGACACCACATCGCAGGTGCCCCAATCCACCCCACGCCAAAGCGGAGATTCAGTCGCGGCCGGCGTCTGGTTACACC‑GCGTTGCAGCCGTTTGCAGACGTGGGGTGAGACATTGTTCATCGTGCGCCATCTGTTACTCCTTAAACAACGCGAACCGc                                                                                                                                                   >  2:361205/1‑251 (MQ=255)
                                                                                             cgcCGAAGAAATCCCAGACGATGCC‑GCCGCGACCCCCAT‑CGGCCCAGTCGAGG‑CATCCCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATc                                                                                                                                                <  1:402300/251‑1 (MQ=255)
                                                                                                cGAAGAAATCCCAGACGATGCC‑GTCGCGAACCACAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACCATGCGAAACGCATCGAc                                                                                                                                             >  1:428500/1‑251 (MQ=255)
                                                                                                 aaaaaaaTCCCAGCCGATGCC‑TTCGCGAACCCCAC‑GGGACCAGGCGAGG‑CATACCGCCACTCGCTGTCCGTCGCGGTCGAGAACCAGCGACCGCACAGCGGAGGTGCCCAGATCCTCCCCCAGCCACAGCGGAGATGCCGTCGCGTCAGGCTTCTGGTTACACATGGGGT‑CCTGCGGTTGCAGACGTGGGGTGAGCCATTGTTCGGCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACt                                                                                                                                            <  1:230088/247‑1 (MQ=255)
                                                                                                         cccAGACGAGCCC‑GGCGCGACCCACAG‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGTAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCTTTTGCAGACGTGTGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATacac                                                                                                                                     <  1:506717/250‑1 (MQ=255)
                                                                                                            aGACGATGCC‑GTCGCGAACCACAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCg                                                                                                                                 >  1:491126/1‑251 (MQ=255)
                                                                                                               cgacgCC‑GTCGCGAACCCCAT‑CGGCCCAGTGGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCGGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACg                                                                                                                              <  1:450174/247‑1 (MQ=255)
                                                                                                                   gcc‑gccGCGAACCACAC‑CGGCCCAGACGAGG‑CATCCCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGa                                                                                                                          <  1:357490/251‑1 (MQ=255)
                                                                                                                       gTCGCGACCCACAT‑CGGCCCAGTAGAGG‑CATACCGCCAATGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGGTTACGACGTTTGCAGTCGTGGGGGGGGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAAAAATGCGAAACGCATCGACTAATACCCAGCGACGCAGACgg                                                                                                                       >  2:348253/1‑251 (MQ=255)
                                                                                                                       gTCGCGAACCACAT‑CGGCCCAGTCGAGG‑CATAACGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCAGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCCTCTGTTACTCCTTAAACAATGCGAAACTCATCGACTAATACCCAGCGCCGCAGACgg                                                                                                                       >  2:419263/1‑251 (MQ=255)
                                                                                                                         cgcgAACCACCT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCCGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCCTCGACTAATACACAGCGCCGCCGGCGGAc                                                                                                                     >  2:480510/1‑251 (MQ=255)
                                                                                                                         cgcgAACCACAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGCCACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGCCGCagacagac                                                                                                                     >  1:358736/1‑251 (MQ=255)
                                                                                                                         cagcgcacagcttcggcacacgctaGG‑CATCCCTCCAATGGCTGCCCGGCGCGGTTGACAACAATCGACACCACATCGCAGGCGCCCAGACCCCCCCCCAGTCACAGCGGAGATTCCGGCGCGGCAGGCGTCTGTTTACACA‑GCGGTGCCGCCGTTTGTAGACGTTGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCTTTAAACAATGCGAAACGCATCGACTAAGACTCAGCGATTCAGACg                                                                                                                        <  2:238932/225‑1 (MQ=255)
                                                                                                                             aaCCACAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACAACACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGATACACA‑GCGTTGCCGCCGTTTGCAGACGAGGGGTGAGCCATTGTTCGTCGCGCGACATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATAAACAGCGACGCAGACGGaacaa                                                                                                                  >  1:38889/1‑246 (MQ=255)
                                                                                                                                cacaT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGCGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCTACTAATACACAGCGACGCAGACGGACAACCGt                                                                                                               >  2:331310/1‑250 (MQ=255)
                                                                                                                                 acaT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTAc                                                                                                             >  1:133663/1‑251 (MQ=255)
                                                                                                                                      cGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATc                                                                                                                                                >  1:493833/1‑212 (MQ=255)
                                                                                                                                       gccccgaggcgagcactcccccaccTGGCTGACCTGCGCGGTCGACACCCAGCGACACCTCGTCGCAGGTGCCCAGACCCACCCCCAGCCACAGCGGAGATTCCGCCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAAGGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAACCGTAcgc                                                                                                           <  2:360058/225‑1 (MQ=255)
                                                                                                                                           cAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGGGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTgg                                                                                                    >  1:159284/1‑251 (MQ=255)
                                                                                                                                            aGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGGTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTCAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGt                                                                                                   >  2:194142/1‑251 (MQ=255)
                                                                                                                                                gAGG‑CCTACCGCCACTGGCTGACCGTCGCGGTCGACAACCACCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTAcc                                                                                               <  2:261849/251‑1 (MQ=255)
                                                                                                                                                 gagccctACCGCCACTGGCTGACCGGCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTAcc                                                                                               <  2:452245/245‑1 (MQ=255)
                                                                                                                                                  gg‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCCGGCGTCTGGTTACCCA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCCTTGTTAGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTACTACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTAcccc                                                                                             >  2:473720/1‑251 (MQ=255)
                                                                                                                                                  gg‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGGCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACCATGCGAAACGCATCGACTAATACCCAGCGACGCAGACGGAACACCGTACGCGCGCGGGTTAcccc                                                                                             >  2:409739/1‑251 (MQ=255)
                                                                                                                                                     cATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCtt                                                                                           >  2:479592/1‑251 (MQ=255)
                                                                                                                                                      atagcGCCACTGACTGAACGGCGCGGTCGACAACCATCGACACCGCATCGCAGGTGCCCAATTCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCCTCTGGTTACAAA‑GCGTTGCCGCCGTTTGCAGACGCGGGGAGAGACATTGTTCGGCGTACGCCATCTGTTACTCCTTAAACACTGCGAAACGACTCGACTAATACAAAACGACGCAGACGGACAAACGTACGCGCGCTGGATACCCCtct                                                                                          >  2:322224/5‑249 (MQ=255)
                                                                                                                                                       tACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGAGGAGATTCCGTCGCGGCAGGCGTCTGGTTACAAA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTCCTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCtccc                                                                                         >  1:61509/1‑248 (MQ=255)
                                                                                                                                                         ccGCTACTGGCTTACCGTCGCGGCCGACAACCATCGACCCCACCTCGCAGGTGCCCAGATCCTCCCCCAGCCTCAGCGGCGATTCCTTCGCGGCAGGCGTCTGGTTCCACA‑TCGTTGCCGCCGTTTGCAGACGTGTTGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTcccc                                                                                       <  2:482137/251‑4 (MQ=255)
                                                                                                                                                         ccGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGCCGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCAcc                                                                                       >  1:374068/1‑251 (MQ=255)
                                                                                                                                                            ccACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGt                                                                                    >  1:239967/1‑251 (MQ=255)
                                                                                                                                                            ccACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGt                                                                                    >  1:396126/1‑251 (MQ=255)
                                                                                                                                                             cACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGtt                                                                                   <  1:397654/251‑1 (MQ=255)
                                                                                                                                                               cTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCACCAGCCACAGCGGAGATTCCATCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGATGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTgg                                                                                 >  1:55803/1‑251 (MQ=255)
                                                                                                                                                                 ggCTGACCGTCGCGGTCGACAACCATCGACACCACAGCGCAGGTGCCCAGAGCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGc                                                                                <  2:479755/250‑1 (MQ=255)
                                                                                                                                                                  gCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGAATCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCGTTTGTTCGTCGTGCGCCCTCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATCAAAAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTAACCCTTCACCGGTTCGCgt                                                                              >  1:513783/1‑251 (MQ=255)
                                                                                                                                                                    tGACCGTCGCGGTCGACAACCATCGACACCACATTGCAGGCGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAATCAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAAAGTACGCGCGCTGGTTACCCCTTCACCGGGTGGCgtgg                                                                            >  2:514286/1‑251 (MQ=255)
                                                                                                                                                                     gACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCCGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACCATGCGAAACGCATCGACTAATACCCCACGACGCCGACGGCCAAACGCACGCGCGCTGGTTACCCCTTCCCCGGTTTGCgtggt                                                                           >  2:436003/1‑251 (MQ=255)
                                                                                                                                                                            gcgGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTc                                                                    >  2:109968/1‑251 (MQ=255)
                                                                                                                                                                             cgGTCGCCAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTc                                                                    >  1:441067/1‑250 (MQ=255)
                                                                                                                                                                               gTCGACAACCATCGACACCCCACCGCAGGGGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCAtg                                                                 <  2:446012/251‑1 (MQ=255)
                                                                                                                                                                                   accaCCATCGACACCACATCTCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCTATGCCGCCGTTTGCAGACGTGGGGGGAGCCATTGTTCGTCGTGCGCCATCTGTTCCTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTAACCGGTTGACGTGGTGCTGGTCAtggtgg                                                             >  1:69676/4‑251 (MQ=255)
                                                                                                                                                                                   aCAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGCCGCAGACGGACAAACGTACGCGCGCTGGTTACCACTTCACCGGTTGGCGTGGTGATGGTCAtggtgg                                                             >  1:9939/1‑251 (MQ=255)
                                                                                                                                                                                        cATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAg                                                        <  2:215611/251‑1 (MQ=255)
                                                                                                                                                                                         aTCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGc                                                       >  1:464282/1‑251 (MQ=255)
                                                                                                                                                                                             acacCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACATCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTc                                                   <  1:198494/251‑1 (MQ=255)
                                                                                                                                                                                                 cacaTCGCAGGTGCCAAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACACACGTACGCGCGCTGGTTACCCCTTCCCCGGTTGGCGTGGTGATGGTAATGGTGGTCCAGCCTTccccc                                               >  1:409096/1‑250 (MQ=255)
                                                                                                                                                                                                    aTCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGccc                                            >  1:161873/1‑251 (MQ=255)
                                                                                                                                                                                                    aTCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAAGCGTCTGGTTACACA‑GCGCTGCCGCCGTTTGCAGACGTg                                                                                                                                                                                                           >  1:34213/1‑92 (MQ=255)
                                                                                                                                                                                                      cGCAGGTGCCCAGATCCCCCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGcccag                                          <  1:153698/251‑1 (MQ=255)
                                                                                                                                                                                                      cGCAGGTGACCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCAGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGccc                                            >  2:525901/1‑249 (MQ=255)
                                                                                                                                                                                                             gCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGcccagccccag                                    <  2:161873/250‑1 (MQ=255)
                                                                                                                                                                                                               ccAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCcagccccagccc                                 >  1:168200/1‑251 (MQ=255)
                                                                                                                                                                                                                cAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCg                                >  1:118711/1‑251 (MQ=255)
                                                                                                                                                                                                                      cACCCCCAGCCACATCGGAGTTTCCGTCGCGGCAGGAGTCTGGTTACGCA‑GCGGTGCCGCCGGTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTTCCCCTTCGCCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCGTCCCCTCCCATTCCCAGCCCGGCAATg                          <  2:456272/251‑1 (MQ=255)
                                                                                                                                                                                                                      cACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTGCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATg                          <  2:201162/251‑1 (MQ=255)
                                                                                                                                                                                                                         ccccAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCAc                       <  1:7175/251‑1 (MQ=255)
                                                                                                                                                                                                                             aGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTCAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCACCCCCAGCCCTGCAATGCACGGTc                   >  1:484484/1‑251 (MQ=255)
                                                                                                                                                                                                                             aGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTc                   <  1:472477/251‑1 (MQ=255)
                                                                                                                                                                                                                               ccACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGGGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCg                 >  1:256730/1‑251 (MQ=255)
                                                                                                                                                                                                                               ccACAGAGGAGATTCCGTCGCGGCCGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCg                 >  2:343400/1‑251 (MQ=255)
                                                                                                                                                                                                                                     cGGAGATTCCGTCGCGGCAGGCGTCTGGTGACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTa           <  2:280275/251‑1 (MQ=255)
                                                                                                                                                                                                                                              cggcgcgGCAGGCGTCGGGTTACACA‑GCGGTGCCGCCTTTTGCGGACGTGGGGTGGGCCATTGGTCGTCGTGCGCCATCGGTTGCGCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGCCGCAGACGGACAAACGGACGCGCGCTGGTGACCCCTTCGCCGGTTGGCGTGGTGATGTGCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAAGGCACTGTTCTTTCTTAACGAAAATg  <  2:26776/248‑1 (MQ=255)
                                                                                                                                                                                                                                              cGTCGCGGCAGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCCTCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCCTGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGAACATg  >  2:36837/1‑251 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  
CCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCC‑GTCGCGAACCACAT‑CGGCCCAGTCGAGG‑CATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGTCTGGTTACACA‑GCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGAAAATG  >  NC_000913/2570025‑2570510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑