Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	321,260	A→T	intergenic (+179/‑348)	rclR → / → ykgE	DNA‑binding transcriptional activator RclR/putative lactate utilization oxidoreductase YkgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	321,260	0	A	T	100.0%	42.8 / NA	14	intergenic (+179/‑348)	rclR/ykgE	DNA‑binding transcriptional activator RclR/putative lactate utilization oxidoreductase YkgE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (3/11);  total (3/11)

TGTACCCCGGGAGAATATCGGGAAAGGGTCAGACAGCTTGCACCCTGAATAAAACCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGAT  >  NC_000913/321034‑321465                                                                                                                                                                                                                                   |                                                                                                                                                                                                              tGTACCCCGGGAGAATATCGGGAAAGGGTCAGACAGCTTGCACCCTGAATAAAACCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTGCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTGTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGt                                                                                                                                                                                        <  1:278838/250‑1 (MQ=255)                        aaGGGTCAGACAGCTTGCACCCTGAATAAAACCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAgtgt                                                                                                                                                                <  1:238411/251‑1 (MQ=255)                                    gCTTGCACCCTGAATAAAACCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATaa                                                                                                                                                    <  1:461937/251‑1 (MQ=255)                                                     aaCCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAATTAGct                                                                                                                                   <  2:510489/251‑1 (MQ=255)                                                        cGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTg                                                                                                                                >  2:126678/1‑251 (MQ=255)                                                          gaaGAAATCAGGGCAAAGATACTCCGACTTTAGGGAGATGTCAACATTTCAACTCTTTCTCTTTACAACGGCGGTTAGGGCTTTATATATTTTTTCTGATGGAATCTAGCTCCGTTTTTAAATACAAATGCAAACTAAATTGCATCACTTAAATAAAATACCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACAATCAGTGTATATCTCAATAACCACTGAATAAGTAGCTCTGa                                                                                                                               >  2:329502/3‑250 (MQ=255)                                                          ccAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTTTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTTAATAAGTAGCTCTGaa                                                                                                                              <  2:37658/251‑1 (MQ=255)                                                                              aattcGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCGGAAGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGAGTATTTTCGGTGATTTTTTTGTACACCATCAGTGTATAGCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGc                                                                                                           <  1:367456/246‑1 (MQ=255)                                                                                        ccGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTaaa                                                                                                 <  1:477436/250‑1 (MQ=255)                                                                                                                            aaCGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTTTGAATAGATATAAGGGATGTAGCCATTTTTAAATGGATTTCTTCTGCCAAAAATGATCGTCGATTTcccc                                                            <  2:79090/251‑1 (MQ=255)                                                                                                                                                                               ttAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATGAGTTATTGCATTTGAc          <  1:126678/250‑1 (MQ=255)                                                                                                                                                                                  aataaaAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATGTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATATGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGGCGAAAGAAATAAAATTAGTTAGTGCATTTGACGttt      <  2:401423/251‑1 (MQ=255)                                                                                                                                                                                     aaaaaTGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTTCTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTgg    >  1:196118/1‑250 (MQ=255)                                                                                                                                                                                      aaaaTGCAAACTAAATTGCTTCAATGAAATAAAATCCCGACTATTTCTTGATACGTGATTATGGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGGGGATGTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTGTGGAt  <  2:537436/251‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                              TGTACCCCGGGAGAATATCGGGAAAGGGTCAGACAGCTTGCACCCTGAATAAAACCGCCAGAAATCAGGGCAAAGATAATCCGCATTCCGGGAGTTGTGAGCCTTTCAACTATTTCTATTTCCAACGGTGGTTTGGGCTTTATATATTTTTTCTGATGGACTCTAGCTCAGTTTTTAAATAAAAATGCAAACTAAATTGCTTCAATTAAATAAAATCCCGACTATTACTTGATACGTGATTATTGTCGGTGATTTTTTTGTACACCATCAGTGTATATCTCAATAACCCCTGAATAAGTAGCTCTGAATAGGTATAAGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGAT  >  NC_000913/321034‑321465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑