Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,520,199	A→G	G158G (GGT→GGC)	gltX ←	glutamate‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,520,199	0	A	G	98.8%	251.9 / NA	82	G158G (GGT→GGC)	gltX	glutamate‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (38/42);  minor base C (1/0);  total (40/42)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTGGACAGTTTTTTACCGTCATCGCCATTGATCATAGAAACGTGCGCGTAAACCGGCACCGGCGCTTTCAGGGCCTTAAGAATGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGACCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTT  >  NC_000913/2519953‑2520443                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     tttGGACAGTTTTTTACCGTCATCGCCATTGATCATAGAAACGTGCGCGTAAACCGGCACCGGCGCTTTCAGGGCCTTAAGAATGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTAATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGGT‑C‑GGGCCAc                                                                                                                                                                                                                                                  >  2:542477/1‑251 (MQ=255)  ttGGACAGTTTTTTACCGTCATCGCCATTGATCATAGAAACGTGCGCGTAAACCGGCACCGGCGCTTTCAGGGCCTTAAGAATGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGAGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACg                                                                                                                                                                                                                                                 >  1:291351/1‑251 (MQ=255)    ggccAGTTTTTTACCGTCATCGCCTGTGATCATAGAAACGTGAGCTCAAACCGGCCCCGGCTCTTTCAGGGCCTTAAGAATGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTCCGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACgg                                                                                                                                                                                                                                                <  2:301393/247‑1 (MQ=255)      aCAGTTTTTTACCGTCATCGCCATTGATCATAGAAACGTGCGCGTAAACCGGCACCGGCGCTTTCAGGGCCTTAAGAATGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATc                                                                                                                                                                                                                                             >  1:41412/1‑251 (MQ=255)            ttttACCGTCATCGCCATTGATCATAGAAACGTGCGCGTAAACCGGCACCGGCGCTTTCAGGGCCTTAAGAATGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCAGCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATc                                                                                                                                                                                                                                        <  2:278332/250‑1 (MQ=255)            ttttACCGTCATCGACATTGATCATAGAAACGTGCGCGTAAACCGGCACCGGCGCTTTCAGGGCCTTAAGAATGTTGAGCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCTGATAATAAGAGCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATc                                                                                                                                                                                                                                        <  2:490866/250‑1 (MQ=255)              ttACCGTCATCGCCATTGATCATAGAAACGGGCGCGTAAACCGGCACCGGCGCTTTCAGGGCCTTAAGAATGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGGTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGt                                                                                                                                                                                                                                      <  1:276209/250‑1 (MQ=255)               tACCGTCATCGCCATTGATCATAGAAACGTGCGCGTAAACCGGCACCGGCGCTTTCAGGGCCTTAAGAATGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTc                                                                                                                                                                                                                                     >  2:5671/1‑250 (MQ=255)                aCCGTCATCGCCATTGATCATAGAAACGTGCGCGTAAACCGGCACCGGCGCTTTCAGGGCCTTAAGAATGTTGATCTGGCGTTGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTGCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGGTGGGGATCCAGCGGTACGGCGGATAATAAGATCGTCCAGGTCCGGGGTGCTGAACTCGAT‑C‑GGGCCCCGGATCTGATCGTCaa                                                                                                                                                                                                                                   <  2:55486/251‑1 (MQ=255)                aCCGTCATCGCCATTGATCATAGAAACGTGCGCGTAAACCGGCACCGGCGCTTTCAGGGCCTTAAGAATGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTAGAT‑C‑GGGCCACGGATCTGATCGTCac                                                                                                                                                                                                                                   >  2:463291/1‑250 (MQ=255)                 ccGTCATCGCCATTGATCATAGAAACGTGCGCGTAAACCGGCACCGGCGCTTTCAGGGCCTTAAGAATGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCaa                                                                                                                                                                                                                                   >  2:159462/1‑250 (MQ=255)                          ccATTGATCATAGAAACGTGCGCGTAAACCGGCACCGGCGCTTTCAGGGCCTTAAGAATGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGa                                                                                                                                                                                                                         <  1:375245/251‑1 (MQ=255)                             ttGATCATAGAAACGTGCGCGTAAACCGGCACCGGCGCTTTCATGGCCTTAAGAATGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAAcc                                                                                                                                                                                                                      >  2:411057/1‑251 (MQ=255)                               gATCATAGAAACGTGCGCGTAAACCGGCACCGGCGCTTTCAGGGCCTTAAGAATGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCtt                                                                                                                                                                                                                    <  1:189720/251‑1 (MQ=255)                               gATCATAGAAACGTGCGCGTAAACCGGCACCGGCGCTTTCAGGGCCTTAAGAATGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCt                                                                                                                                                                                                                     <  1:177886/250‑1 (MQ=255)                                   aTAGAAACGTGCGCGTAAACCGGCACCGGCGCTTTCAGGGCCTTAAGAATGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTg                                                                                                                                                                                                                <  2:135750/251‑1 (MQ=255)                                           gTGCGCGTAAACCGGCACCGGCGCTTTCAGGGCCTTAAGAATGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGtt                                                                                                                                                                                                          >  1:158104/1‑249 (MQ=255)                                            tGCGCGTAAACCGGCACCGGCGCTTTCAGGGCCTTAAGAATGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGACAAAAACAACAGAACCTTCCTGCGGGTTAGc                                                                                                                                                                                                       >  1:290988/1‑251 (MQ=255)                                                 gTAAACCGGCACCGGCGCTTTCAGGGCCTTAAGAATGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAAc                                                                                                                                                                                                  >  1:23856/1‑251 (MQ=255)                                                     accggcaccggcGCTTTCATGGCCTTAAGAATGTTGATCTGGCGGGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTac                                                                                                                                                                                              <  2:303102/251‑1 (MQ=255)                                                              ggcGCTTGCAGGGCCTTAAGAATGTTGATCTGGCGGGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCGGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACgg                                                                                                                                                                                     <  2:82521/251‑1 (MQ=255)                                                                 gcTTTCAGGGCCTTAAGAATGTTGATCTGGCGGGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGACCCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTtc                                                                                                                                                                                  <  1:406052/251‑1 (MQ=255)                                                                    ttCAGGGCCTTAGGAATGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTtcatc                                                                                                                                                                               >  1:93706/1‑251 (MQ=255)                                                                       aGGGCCGTAAGAATGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGGGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTtcatcatc                                                                                                                                                                            <  2:276908/251‑1 (MQ=255)                                                                          gCCTTAAGAATGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGG‑AACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGcc                                                                                                                                                                        >  2:134607/1‑250 (MQ=255)                                                                           ccTTAAGAATGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCAGATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCa                                                                                                                                                                        <  1:144013/251‑1 (MQ=255)                                                                              tAAGACTGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGCTAACGTGGGTGATTTCCATATCCCAGTAATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCCAAACGTACAACACACGGTTCATCATCAGCatg                                                                                                                                                                      >  2:72809/1‑250 (MQ=255)                                                                               aaGAATGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGATCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGGTCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCCAAAAGTCCCACCCACGGTTCATCATCAGCatgat                                                                                                                                                                    >  2:449443/1‑251 (MQ=255)                                                                                   aTGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTc                                                                                                                                                                <  2:153247/251‑1 (MQ=255)                                                                                       tGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATg                                                                                                                                                             <  1:133514/250‑1 (MQ=255)                                                                                          tCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCtg                                                                                                                                                         >  2:543515/1‑251 (MQ=255)                                                                                                gtggcgtgTTGTTGATATGGTCTTCGCCACGGGTAACGGGGGTGATTTCCATATCCCAGTCATCGACCACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATACGATCGTCCAGTTCCTGGTTGCTGAACTCGCT‑CCGGCCCA‑GGATCTGATCGTCAAAAACAAACGCACCTTCCTGCGGGATAGCAAAACGTACAACACAAGGATCCTCTTCAGCATGATGCTCCTGGCTGTtgcgg                                                                                                                                                   >  1:405991/1‑251 (MQ=255)                                                                                                                atGGTCGTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTTGTGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCTAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCACATGGCTGTGGCGGCAGCGACCGTCATAAc                                                                                                                                   <  2:405991/251‑1 (MQ=255)                                                                                                                    tCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGCTAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAAAGCgg                                                                                                                               >  1:495868/1‑251 (MQ=255)                                                                                                                     cTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATACGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGAAGCGACCGTCATAACGCGGc                                                                                                                              >  2:482206/1‑251 (MQ=255)                                                                                                                       tCGCCACGGATAACGTGGGTGAGTTCCATAGCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGc                                                                                                                              <  2:92730/249‑1 (MQ=255)                                                                                                                              ggATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGGTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAACGACCGTCATAACACGGCttttctcct                                                                                                                     >  2:3229/1‑244 (MQ=255)                                                                                                                                        ggTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCAttt                                                                                                           <  2:93706/251‑1 (MQ=255)                                                                                                                                         gTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCAAGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCTCCTTTCGCCATTTg                                                                                                          >  1:103534/1‑251 (MQ=255)                                                                                                                                          tGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGc                                                                                                         >  1:423002/1‑251 (MQ=255)                                                                                                                                           gATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGc                                                                                                         <  2:438669/250‑1 (MQ=255)                                                                                                                                             tttCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAAc                                                                                                                                                                                                  >  2:168701/1‑159 (MQ=255)                                                                                                                                             tttCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTGCCTGCGGGTTAGCAAAAc                                                                                                                                                                                                  <  1:168701/159‑1 (MQ=255)                                                                                                                                              ttCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCTGCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCtt                                                                                                     >  1:130855/1‑251 (MQ=255)                                                                                                                                               tCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCgg                                                                                                                               <  2:244891/224‑1 (MQ=255)                                                                                                                                               tCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCgg                                                                                                                               >  1:244891/1‑224 (MQ=255)                                                                                                                                                 cATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAACATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTcgc                                                                                                  >  1:48632/1‑251 (MQ=255)                                                                                                                                                      cccAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAgc                                                                                             <  1:377348/251‑1 (MQ=255)                                                                                                                                                      cccAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGACGGATAATAAGATCGTCCAGGTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTAAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAACGACCGTCATAACGCGGCTTTTCACCTTTCGCCCTTTGCTCTTCGCGCAgc                                                                                             >  1:356458/1‑251 (MQ=255)                                                                                                                                                       ccAGTCAGCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAgcg                                                                                            <  1:482206/251‑1 (MQ=255)                                                                                                                                                       ccAGTCAACGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAgcg                                                                                            <  2:94534/251‑1 (MQ=255)                                                                                                                                                        cAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTTGCCATTTGCTCTTCgcgccgagc                                                                                           >  2:136217/1‑246 (MQ=255)                                                                                                                                                            cATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTcc                                                                                       <  2:134403/251‑1 (MQ=255)                                                                                                                                                             aTCGACAACCACACAGAAGTTATAGGTTGGGGCACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCaaa                                                                                                                                                                                                    >  1:412271/1‑141 (MQ=255)                                                                                                                                                             aTCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCaaa                                                                                                                                                                                                    <  2:412271/141‑1 (MQ=255)                                                                                                                                                               cGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTGCATCATCAGCATGATGCTCATTGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAgg                                                                                    <  1:268393/251‑1 (MQ=255)                                                                                                                                                                gACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGgc                                                                                   <  1:551262/251‑1 (MQ=255)                                                                                                                                                                      cacacaGAAGTTCTAGGTTGGGGAACCATCGGTACGGCGGATCATAAAATCGTCCAGTTCCTGGTTCCTGAACTCGATTC‑GG‑CCACGGAACTGTCCGTCAAAAACAACAGAACCTTCCTCCGGGTTAGCACAACGTACAACACACGGTTCATCATCAGCATGATGCTTATGGCTGTGGCGGCAACGACCGTCACAACGCGGCCTCCCACCTTTTGCCACTTGCTCTTCGCTCAGCCCTTcccaacgttctt                                                                             >  1:228751/1‑241 (MQ=255)                                                                                                                                                                       acacaGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGCTCGTCCAGTTCCTGGTTGCTGAACTCGCT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACCCACGGTTCATCATCCACATGATGCTCATGGCTGTGGCGGCCGCGACCGTCATAACGCGGCTTTTCACCTTTCGCCATTTGCTCTTCGCGCCGCGCTTCCCGGCGCTCttt                                                                            >  2:201597/1‑251 (MQ=255)                                                                                                                                                                        cacaGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGATGCACTCGAT‑C‑GGGCCACGGATCTGATAGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGGCAAAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCttt                                                                            >  1:522884/1‑250 (MQ=255)                                                                                                                                                                             aaGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGACCCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGc                                                                       >  1:377649/1‑250 (MQ=255)                                                                                                                                                                                  ataGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAg                                                                  <  1:72809/250‑1 (MQ=255)                                                                                                                                                                                    aGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCa                                                                <  1:397022/250‑1 (MQ=255)                                                                                                                                                                                           ggAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAg                                                        <  1:542477/251‑1 (MQ=255)                                                                                                                                                                                           ggAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAg                                                        <  1:159462/251‑1 (MQ=255)                                                                                                                                                                                            gAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTCAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACCACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCCTGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGc                                                       >  1:204969/1‑251 (MQ=255)                                                                                                                                                                                               ccATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAgg                                                    >  1:412591/1‑250 (MQ=255)                                                                                                                                                                                               ccATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCAGCACGTCAAAACCAACCGAACCTCCCTGCGGCTTAGAAAAACGTACAACACCCGGTTCATCATCAGCCTGATGCTCATGGCTGTGGCGGCCGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTATTCGCGCAGCGCTTTCAGGCGCTCTTTCGCGCAATAGCATTTATaaaccg                                                     >  1:229146/1‑246 (MQ=255)                                                                                                                                                                                                       aCGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAG‑C‑GGGCCACGGAGCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGGACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGGGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCctct                                             <  2:158104/250‑1 (MQ=255)                                                                                                                                                                                                        cggcggATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTc                                           >  1:42108/1‑251 (MQ=255)                                                                                                                                                                                                           cggATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTGCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAg                                        <  2:75239/251‑1 (MQ=255)                                                                                                                                                                                                            ggATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGc                                       <  1:159238/251‑1 (MQ=255)                                                                                                                                                                                                                   aaGAACGTCCAGTTCCTGGTTGCTTACCTCTAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTGCCTGCGGGTTAGCAAAACGTACAACACACGGTTCAGCATCAGCATGATGCTCATGGCTGTGGCGGCATCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCATAGCCCTCTTCCAGCATCTgat                                <  2:262371/251‑1 (MQ=255)                                                                                                                                                                                                                    aGATCGTACAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTgatc                               <  1:23088/251‑1 (MQ=255)                                                                                                                                                                                                                        cGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTgatcgatc                           <  1:411057/251‑1 (MQ=255)                                                                                                                                                                                                                           ccAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCAcc                        >  2:451594/1‑251 (MQ=255)                                                                                                                                                                                                                           ccAGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCAcc                        >  1:206691/1‑251 (MQ=255)                                                                                                                                                                                                                             aGTTCCTGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGACCCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACcgc                      <  1:388361/251‑1 (MQ=255)                                                                                                                                                                                                                                   tGGTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCCGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGt                 >  2:198533/1‑250 (MQ=255)                                                                                                                                                                                                                                    ggTTGCTGAACTCGAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCACCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGAGCACCGCGTTGTAg               <  2:377649/251‑1 (MQ=255)                                                                                                                                                                                                                                                cGAT‑C‑GGGCCACTGATCTGATCGTCAAAAACAACAGAACCGTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTGTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTGTAGAGCAATAGCATTTATAAGCAGTGCTCTCTGCCAGCATCTGATCGATCACCGCGTTGTGGCGATCAAAACg    <  2:270389/250‑1 (MQ=255)                                                                                                                                                                                                                                                 gAT‑C‑GGGCCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGCTCACCGCGTTGTAGCGATCAAAACGtt  >  2:147791/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     TTTGGACAGTTTTTTACCGTCATCGCCATTGATCATAGAAACGTGCGCGTAAACCGGCACCGGCGCTTTCAGGGCCTTAAGAATGTTGATCTGGCGTGGCGTGTTGTTGATATGGTCTTCGCCACGGATAACGTGGGTGATTTCCATATCCCAGTCATCGACAACCACACAGAAGTTATAGGTTGGGGAACCATCGGTACGGCGGATAATAAGATCGTCCAGTTCCTGGTTGCTGAACTCGAT‑C‑GGACCACGGATCTGATCGTCAAAAACAACAGAACCTTCCTGCGGGTTAGCAAAACGTACAACACACGGTTCATCATCAGCATGATGCTCATGGCTGTGGCGGCAGCGACCGTCATAACGCGGCTTCTCACCTTTCGCCATTTGCTCTTCGCGCAGCGCTTCCAGGCGCTCTTTAGAGCAATAGCATTTATAAGCAGTGCCCTCTTCCAGCATCTGATCGATCACCGCGTTGTAGCGATCAAAACGTT  >  NC_000913/2519953‑2520443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑