Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,404,402	G→A	S71L (TCG→TTG)	nuoB ←	NADH:quinone oxidoreductase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,404,402	0	G	A	100.0%	171.3 / NA	47	S71L (TCG→TTG)	nuoB	NADH:quinone oxidoreductase subunit B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/24);  total (23/24)

ACATCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATAATCCATCTTAATGCCTCGCGGTTAGCGTTGACGAT  >  NC_000913/2404156‑2404642                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 acaTCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGt                                                                                                                                                                                                                                              >  1:310016/1‑251 (MQ=255)      aaCCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCAcc                                                                                                                                                                                                                                          >  1:495320/1‑250 (MQ=255)                    tATCGACGCCCTGCACAACGGAATAAATATCGTACATAACACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAAca                                                                                                                                                                                                                            <  2:157655/250‑1 (MQ=255)                          cGCCCTGCACAACGGAATCAATATCGTACATACCACCAGAGTTGGCAAAGGAACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGa                                                                                                                                                                                                                      >  1:31786/1‑250 (MQ=255)                          cGCCCTGCACAACGGAAGAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCAGCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGaa                                                                                                                                                                                                                     <  2:233578/251‑1 (MQ=255)                                gCACAACGGAATAAAGATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCAGCAGGTCAGCCGGACGCGGCGAAGCACGAAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAAAAAAGTCACCATCTCAACGTATCAGCAGGAAAGAcc                                                                                                                                                                                                                <  2:229468/250‑1 (MQ=255)                                                     aCATACCACCAGAGTTGGCACAGGCACCCATGGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAg                                                                                                                                                                                          <  2:207425/251‑1 (MQ=255)                                                       aTACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGtt                                                                                                                                                                                        >  1:440386/1‑251 (MQ=255)                                                               agagTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGAc                                                                                                                                                                                >  2:278349/1‑251 (MQ=255)                                                               agagTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGAc                                                                                                                                                                                >  1:501007/1‑251 (MQ=255)                                                                gagTTGGCACAGGCACCCATTGAGATAGCCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACCTCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGAcc                                                                                                                                                                               <  1:274102/251‑1 (MQ=255)                                                                 agTTGGCACAGGCACCCATTGCGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCCCGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGCAGTTATACGGCCAACTTGAGTTTTTACGAccc                                                                                                                                                                              >  2:48212/1‑251 (MQ=255)                                                                  gTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACTCGGCGAATCACGCAATACGTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTCCGAcccc                                                                                                                                                                             <  2:31786/251‑1 (MQ=255)                                                                     ggCACAGGCACCCATTGAGATAACCCATGTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTGTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGTTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGt                                                                                                                                                                          <  1:224470/251‑1 (MQ=255)                                                                          aGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCACATTGAGTTTTTACGCCACAAGTTAAcc                                                                                                                                                                     >  2:179405/1‑251 (MQ=255)                                                                            gCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCAt                                                                                                                                                                   <  1:379265/251‑1 (MQ=255)                                                                              aCCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGt                                                                                                                                                                 >  2:547995/1‑251 (MQ=255)                                                                              aCCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTCGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGt                                                                                                                                                                 >  1:55939/1‑251 (MQ=255)                                                                                    tGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTg                                                                                                                                                            >  1:91385/1‑250 (MQ=255)                                                                                          aaCCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGATAACCATGTCATTGAGCTTGc                                                                                                                                                     >  2:62868/1‑251 (MQ=255)                                                                                                      ttCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGt                                                                                                                                         >  1:100205/1‑251 (MQ=255)                                                                                                                 ggTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCATGCCGCGGCGCGGCACGCAATACTCCTGCGCCAACACGCGCCACGTCATGCACCGCGGCAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGACGTTGTACGGCCAAATTGAGttttt                                                                                                                                                                                     >  1:219940/1‑196 (MQ=255)                                                                                                                 ggTCATACAGACGCTGAAGAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCATCCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCGGCGCCAAAACGCGCCACGTCATGCACCGCGGGAAACAAAGTCACCATCTCAACGTAACAGGAGGAAAGACCAAAGTGATACGGCCAAACTGAGttttt                                                                                                                                                                                     <  2:219940/196‑1 (MQ=255)                                                                                                                      tACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTg                                                                                                                         >  2:273703/1‑251 (MQ=255)                                                                                                                       aCAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTg                                                                                                                         <  1:81452/250‑1 (MQ=255)                                                                                                                       aCAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTg                                                                                                                         <  2:278927/250‑1 (MQ=255)                                                                                                                         aGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATATACACGTTTTTGTTAACTTCTTGCTc                                                                                                                      >  2:331763/1‑251 (MQ=255)                                                                                                                          gACGCTGAATAACCGGTGCCATTTTGGTAAAGCTGTTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCTGTAAACAAAGGCGCCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCGTGTCATTGAGCTTGCTCATAAACTCGGTTGTTTTAACGTCTTGCTcc                                                                                                                     <  2:29329/251‑1 (MQ=255)                                                                                                                               tGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAgg                                                                                                                >  1:171187/1‑251 (MQ=255)                                                                                                                                gAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAggg                                                                                                               <  2:91385/251‑1 (MQ=255)                                                                                                                                         gTGCCATTTTGGTAAAGCAGGTTCCTGCCACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACTCGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACg                                                                                                      <  1:480102/251‑1 (MQ=255)                                                                                                                                                                ccTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTa                                                                               <  1:273703/251‑1 (MQ=255)                                                                                                                                                                          ccaTCAGGTCAGCCTGAAGCGGCGAAGCAAGCACTACTTCTGCGCAAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTAATTGCGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTtc                                                                     >  1:180339/1‑251 (MQ=255)                                                                                                                                                                               aGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCAGTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCTTTCTCAcc                                                                <  2:69027/251‑1 (MQ=255)                                                                                                                                                                                 gTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCTCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGTTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGt                                                              <  1:390524/251‑1 (MQ=255)                                                                                                                                                                                              ggcgAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCg                                                 <  2:495320/251‑1 (MQ=255)                                                                                                                                                                                                  aaGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTg                                             <  1:379116/251‑1 (MQ=255)                                                                                                                                                                                                  aaGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGGCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTg                                             <  2:171187/251‑1 (MQ=255)                                                                                                                                                                                                  aaGCACGCAATACTTCGGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGTGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTg                                             >  2:290365/1‑251 (MQ=255)                                                                                                                                                                                                   aGCACGCAATACTTCTGCGCCAAAACGCGCCACTTCATGCTCCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCTCATAAACACGTTTTTGTTAACTGCTTGCTCCAGAGGGTCGGTTACGATCGCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCCCCGTCGGGATCTATggtggtga                                            <  2:248525/251‑8 (MQ=255)                                                                                                                                                                                                       cGCAATTCTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGATGTTATACGGCTAAAGTGAGTTTTGACGACCCCAGTTAACCATGTCATTGAGc                                                                                                                                                         <  2:551327/138‑1 (MQ=255)                                                                                                                                                                                                       cGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGCCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGc                                                                                                                                                         >  1:551327/1‑138 (MQ=255)                                                                                                                                                                                                        gCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGt                                        >  1:20652/1‑250 (MQ=255)                                                                                                                                                                                                                tCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCCACGTAACAGCAGGCAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCCGTTAACCATGTCACAGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGAGAGCGTATAATCCAt                               >  1:38445/1‑251 (MQ=255)                                                                                                                                                                                                                         aaaCGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATAATCCATCTTAATGcc                      >  1:548329/1‑251 (MQ=255)                                                                                                                                                                                                                                          cACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTACTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATAATCCATCTTAATGCCTCGCGGTTAGCGTTGAc     <  2:195598/251‑1 (MQ=255)                                                                                                                                                                                                                                             cgcgGTAAACAAAGTCACCATCTAAACGTAACAGCAGGAAAGACCGAAGTTATAAGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGCTCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGAGCTATGCGGGTGAGCGTATAATCCATCTTAATGCCTCGCGGTTATCGTTGACGAt  <  2:383681/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 ACATCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATAATCCATCTTAATGCCTCGCGGTTAGCGTTGACGAT  >  NC_000913/2404156‑2404642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑