Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,361,210	C→G	E69Q (GAA→CAA)	rhmD ←	L‑rhamnonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,361,210	0	C	G	95.7%	161.3 / ‑5.5	47	E69Q (GAA→CAA)	rhmD	L‑rhamnonate dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (23/21);  minor base A (1/1);  total (25/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGAC  >  NC_000913/2360972‑2361454                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     aCCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGt                                                                                                                                                                                                                                          >  1:421296/1‑251 (MQ=255)           cgcccagatcaaacaGAGACAGATCGACAAAAGAAATCGTATTCATCCCCAGGCCACCAGAGCCGGAGTAATACAGGGTGGAACTGCACATTTGATCGCGGATCAGTTTGATATCACTGACACCTTTACCATCATTGAAACGGTTCAGATGTTTTTCGACAATAACACAGCGCATTTCCACTGCTGTCGAAACGACGAATCCGGGCTTGCCGTTTTCATCTTCGCGTTTCACAACCAACGGGCCAAGaa                                                                                                                                                                                                                                  >  2:221017/13‑249 (MQ=255)             aaCAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTGCACCGGCTGGCGAAACGGCGAAGCCGGTCTGGCCGTTTTCTGCGTCGACTTGAACAACCAACGGGCCAAGAACGtt                                                                                                                                                                                                                              <  2:95950/251‑1 (MQ=255)                    cccACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCAACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGcc                                                                                                                                                                                                                        >  1:250436/1‑250 (MQ=255)                     ccACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCaa                                                                                                                                                                                                                      <  1:56051/251‑1 (MQ=255)                       acaGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAAt                                                                                                                                                                                                                    <  1:263491/251‑1 (MQ=255)                         agagCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGa                                                                                                                                                                                                                  <  1:358362/251‑1 (MQ=255)                                                 gTATTCATCACCAGGCCACCAGAGCCTGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGGAATcgcg                                                                                                                                                                                          <  1:407435/251‑1 (MQ=255)                                                 gTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATcgcg                                                                                                                                                                                          >  2:453520/1‑251 (MQ=255)                                                  tATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGCTGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTACTGCCAAATGACTGGCGTGACTGCTCGTAATCGCgg                                                                                                                                                                                         >  2:532674/1‑251 (MQ=255)                                                      catcaCCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTAtt                                                                                                                                                                                     <  1:437044/251‑1 (MQ=255)                                                        tcaCCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTAttt                                                                                                                                                                                    >  1:457877/1‑250 (MQ=255)                                                            cAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTc                                                                                                                                                                                >  1:142997/1‑250 (MQ=255)                                                                  accaGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCgggg                                                                                                                                                                         <  2:222574/251‑1 (MQ=255)                                                                  accaGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCgggg                                                                                                                                                                         <  1:29783/251‑1 (MQ=255)                                                                  accaGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCgggg                                                                                                                                                                         >  1:82419/1‑251 (MQ=255)                                                                       agCCGGAGTAATACAGGGTGGCACCGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTCAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGCTCGTTAATGCCAAATGACTGGCGTGACTGCTAGTAATCGCGGCATTTACTCATCGGGGTGGc                                                                                                                                                                     >  2:29150/1‑250 (MQ=255)                                                                              gTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGAt                                                                                                                                                             <  1:138836/251‑1 (MQ=255)                                                                                    cAGGGGGGCCCTGAGCATGTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGTCGTGACTGCTCGTAATCGCGGTATTTACTCATCTGGGTGGCAATATGATCGTCTa                                                                                                                                                       <  2:406522/251‑1 (MQ=255)                                                                                       ggTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATcc                                                                                                                                                    <  1:402930/251‑1 (MQ=255)                                                                                             aCTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGAt                                                                                                                                              >  2:303425/1‑251 (MQ=255)                                                                                                gAGCATTTGATCGTGGATCAGTTTGATATCACTGACACAATTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAAGCCGGTCTGGCCGTTTTCTGCTGCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTAGCCAGTGATTc                                                                                                                                            <  2:238866/250‑1 (MQ=255)                                                                                                 aGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCa                                                                                                                                                                               <  1:341702/214‑1 (MQ=255)                                                                                                 aGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGAATGCTCGTAATCGCGGTATTTACTCa                                                                                                                                                                               >  2:341702/1‑214 (MQ=255)                                                                                                     tttGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCACTGAAACGGTTAAGATGTTTTTCGACAATAAAAAAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTACTTCGACTTGAACACCCAACGTGCCAAGAACGTTACTGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCACTATCATCCTCAATcccgtcattgggcccc                                                                                                                                      >  1:316860/1‑237 (MQ=255)                                                                                                         aTCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTCAGATGTTTTTCGACAATAAAACCGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGGGATTCGCCCCCTGGt                                                                                                                                  >  1:480596/1‑251 (MQ=255)                                                                                                                  cAGTTTGATATCACTGACACATTTACCCGCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAAt                                                                                                                         <  1:402279/251‑1 (MQ=255)                                                                                                                   aGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATc                                                                                                                        <  1:193531/251‑1 (MQ=255)                                                                                                                        gATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCAccg                                                                                                                    <  1:188335/250‑1 (MQ=255)                                                                                                                         atatCACTGACACATTTACCCGCAATGAAACGGTTAAGATGTTTGTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTTCGTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAAGGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGGGGCAATATGATCGGCTATCCAGTGATTCGCCCCTTGGTCTTGAGAATCAccgcc                                                                                                                  <  2:250436/251‑1 (MQ=255)                                                                                                                               cTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAACTGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAgc                                                                                                            >  2:170408/1‑251 (MQ=255)                                                                                                                                   cacaTTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCt                                                                                                        >  2:368143/1‑251 (MQ=255)                                                                                                                                     caTTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCACATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCCAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCAACGCCGCCAGCGCCttt                                                                                                      >  1:84432/1‑251 (MQ=255)                                                                                                                                        ttACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCttttt                                                                                                    <  1:368143/250‑1 (MQ=255)                                                                                                                                            ccgcAATGAAAGGGTAAAGATGTTTGTCGCCAATAAAACAGCCCATTTCACCGGCTGAAAAACCGGCGAATCCGGTCTGGCCGTTTCCTGCTTCGACTTGAACACCCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGCCTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCATTGATTCGCCCCCTGGTCGGGATAATCACCGCCGCCAGCGCCTTTTTctgcc                                                                                               <  2:423670/248‑2 (MQ=255)                                                                                                                                                                            aTAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCCGGCGCGGACCTg                                                               >  1:3578/1‑251 (MQ=255)                                                                                                                                                                                  cAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGGATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACC‑CCGCCAACGCCTTTTTCTCCTGTCGCACCGCCAGGAAACCCGgcgggcacccggttaatt                                                        >  1:74524/1‑236 (MQ=255)                                                                                                                                                                                       cATTTCTCCAGCTGCCGATACGGAGAAACCGGTCTGGCCGTTTTCTGATTCGACTTGCACAAACACCGTGCAAAGAACGCTAAAGCCAAATCACTGGAGTGACTGCCCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGAGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACAGCCAGTAAACCAGGCGCGAACATGTTTAATTTTTg                                                    >  2:361592/1‑251 (MQ=255)                                                                                                                                                                                            cACCGGCTGTCGAAATGGCGAATCCGGTCTGGCCGCTTTCTGCTTCGACTTGAACAACCAACGCGCCACGACCGTTAATGTCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTAATCATCGGGGTGGCAATAGGCTCGTCTCTCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCACCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCCGGCGCGAACCTGTTTCCTTTTTGGTAgg                                               >  2:364897/1‑251 (MQ=255)                                                                                                                                                                                                          aCGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAACTGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCCGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCCTGCTGTtctc                                 >  2:515506/1‑251 (MQ=255)                                                                                                                                                                                                                   ccGGTCTGGCCGTTTTCTGCTCCGACTTGAACACCCAACGTGCCAAGAACTTTAATGCAAAATGGCGGGCTTGATTGCTCGTAATCGCGGTTTTTCCTCAGCGGGGGTGAAATATGATCTTCTATCCAGTAGTTCCCCCCCGGGGCGTTAAAATCCCCTCCGCCAGCGCCGTTTTCTGGTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTAt                        <  1:343121/251‑1 (MQ=255)                                                                                                                                                                                                                     ggTCTGGCCTTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCg                                                                                                                                <  1:48231/145‑1 (MQ=255)                                                                                                                                                                                                                     ggTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCg                                                                                                                                >  2:48231/1‑145 (MQ=255)                                                                                                                                                                                                                               ttttCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGCATTTACTCATCGGGGTGGCAATATGATCGTCTATCAAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTa              >  1:365179/1‑249 (MQ=255)                                                                                                                                                                                                                                 ttCTGTTTCGACGTAAACAACCAACGTGCCAAGAGCGTAAATGCCAAAGGACTGGCGTCATTGCTCGAAACCGCGGTATTTACTCAGCGGGGTGGAAATATGATCTTCTCTCCAGTGATTCCCCCCCTGGTCGTAATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGTTCTCCATTGt                           <  2:92482/234‑1 (MQ=255)                                                                                                                                                                                                                                 ttCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGTTCTCCATTGt                           >  1:92482/1‑234 (MQ=255)                                                                                                                                                                                                                                         cGACTTAAACAACAAACGAGCCAAGACCGTTAATGCCAAATGCCTGGCGTGACTGCTCGTAATCGCGGTATTTACTCACCGGGGAGGCAATATGATAGTCTATCCAGTGCTTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCCCCGCCAGTAAACCAGGCGCGACCCTGTTTAATTTTTGGAAGGGTCCTGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGAc  >  2:246077/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     ACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGAC  >  NC_000913/2360972‑2361454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑