Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,247,134	A→C	Y467D (TAC→GAC)	lysP ←	lysine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,247,134	0	A	C	100.0%	159.3 / NA	45	Y467D (TAC→GAC)	lysP	lysine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (30/15);  total (30/15)

CGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACCGCGACGGAAGCGATAGTGGCTAATGGCAATCCCCAGC  >  NC_000913/2246895‑2247375                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  cGGAACAGCCTCTGATTTGACAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACACTTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCACGTAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGTAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCaa                                                                                                                                                                                                                                         >  2:54195/1‑250 (MQ=255)              gATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAAc                                                                                                                                                                                                                             >  1:513093/1‑249 (MQ=255)                          ttttttGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAat                                                                                                                                                                                                               >  1:218231/1‑251 (MQ=255)                             tttGATTGCGGCGTAACGATAAAGCAAACGATAACAATTATCAATCCAAATGTTAAACTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGAAACAGCGGGATACCAatat                                                                                                                                                                                                             >  2:256311/1‑250 (MQ=255)                                         gTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTAc                                                                                                                                                                                                >  1:482775/1‑251 (MQ=255)                                             cGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTAcgccg                                                                                                                                                                                            <  2:482775/251‑1 (MQ=255)                                                      aaaCGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGGCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATGTCTTATCGTTCTGCGGGAACTTCATTTCGCTGAAGCGTACGAAGTGAGGTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGAGACCAATGTGCGTTGCCTCTGGGCCGCCCCAGTCa                                                                                                                                                                                   <  2:226251/251‑1 (MQ=255)                                                           aTAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGt                                                                                                                                                                              >  1:191625/1‑251 (MQ=255)                                                                    aTCAAACCAAATGGTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCACTCTCGGATGCGAGGGGTTAGAAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCGCCAGTCAATAGTATCTTTc                                                                                                                                                                       >  1:72654/1‑249 (MQ=255)                                                                       aaTCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCg                                                                                                                                                                                                    >  1:213211/1‑217 (MQ=255)                                                                       aaTCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCg                                                                                                                                                                                                    <  2:213211/217‑1 (MQ=255)                                                                          ccAAATGTTAAATTGTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTGCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGc                                                                                                                                                               <  1:283359/251‑1 (MQ=255)                                                                                    aaTTTGATATCCGCCTGATTTGGCAAGGACATGGTCTTGCAATAGCTTTCTCGGATGAGAGAGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGAGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGATTGTCGCCGCACCAAATACTCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTAAATAGCATCTTTCAGGAACGCTTCGTAGtt                                                                                                                                                      >  2:496897/1‑250 (MQ=255)                                                                                     aTTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAAGAGTATCTTTCAGGAACGCTTCGTAGTTCt                                                                                                                                                    <  2:218231/251‑1 (MQ=255)                                                                                          atatCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGccc                                                                                                                                               >  2:260038/1‑251 (MQ=255)                                                                                           tatCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGccc                                                                                                                                               >  1:151534/1‑250 (MQ=255)                                                                                              ccGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTg                                                                                                                                                                                                       >  2:65056/1‑191 (MQ=255)                                                                                              ccGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTg                                                                                                                                                                                                       <  1:65056/191‑1 (MQ=255)                                                                                                      tttGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATc                                                                                                                                   >  2:12878/1‑251 (MQ=255)                                                                                                             gggAAATGGAATTGAAAAAGCCCTCTCGGTTGAGCGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGacac                                                                                                                             >  1:136748/1‑250 (MQ=255)                                                                                                                             aaaGCCCACTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACGTATTTCTTATCGTTCTGCGGGAACTTCATGTCGCTGTAGCGTACGAAGGGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATATGTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAAGGAAGGCGAAGAt                                                                                                            <  2:87208/251‑1 (MQ=255)                                                                                                                                  ccTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTAATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATAAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAACGTGATAATCAGACACAGAATGACTGCGAAGATCGGcc                                                                                                       >  1:234831/1‑251 (MQ=255)                                                                                                                                     ctcGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGGGATAATCAGACACAGAATGAATGCGAAGATCGGcccc                                                                                                     >  2:16403/1‑250 (MQ=255)                                                                                                                                             agagGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGga                                                                                              >  1:521037/1‑249 (MQ=255)                                                                                                                                                      aGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGCATGAATGCGAAGATCGGCCCCCGTGGGAAGAAACCTGac                                                                                   >  2:89285/1‑250 (MQ=255)                                                                                                                                                       gCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAACTAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCACCAGTGGGAAGAAACCTGAAc                                                                                  >  1:451233/1‑251 (MQ=255)                                                                                                                                                       gCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAAc                                                                                  <  1:89285/251‑1 (MQ=255)                                                                                                                                                          aggaaggGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGt                                                                               >  1:398904/1‑251 (MQ=255)                                                                                                                                                            gaaggGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTAc                                                                             <  2:151534/251‑1 (MQ=255)                                                                                                                                                                     aaaCTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTATCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATct                                                                    <  1:482826/251‑2 (MQ=255)                                                                                                                                                                     aaaCTTATTTCTTATCGTTCTGCGGAAACGTCATTTCGCTGTAGCGTAAGAAGTTAGTTCCTTTACTCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATAACAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATCATCAGACACAGAATGACTGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGc                                                                          >  2:80379/1‑245 (MQ=255)                                                                                                                                                                                 tATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCg                                                           <  1:12878/248‑1 (MQ=255)                                                                                                                                                                                   tCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTcc                                                      >  1:490215/1‑251 (MQ=255)                                                                                                                                                                                    agTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAAAGGAACGGCAGATCGTTAATGTCGTGTccc                                                     >  2:424789/2‑251 (MQ=255)                                                                                                                                                                                     gTTCTGCGGGAACGCCATGTCGCTGTAGCGTACGAAGTGAGTTCCGTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATTCGTTGCCGATACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATAGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCt                                                    <  2:294006/251‑1 (MQ=255)                                                                                                                                                                                               aaCTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTa                                          <  2:499267/251‑1 (MQ=255)                                                                                                                                                                                                 cTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCttt                                                                                                                                                                        <  2:432185/123‑1 (MQ=255)                                                                                                                                                                                                 cTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCttt                                                                                                                                                                        >  1:432185/1‑123 (MQ=255)                                                                                                                                                                                                   tCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAAc                                        <  2:521037/249‑1 (MQ=255)                                                                                                                                                                                                           cTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACCGCGACGGa                               >  1:543258/1‑250 (MQ=255)                                                                                                                                                                                                           cTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACCGCGACGGa                               >  2:135902/1‑250 (MQ=255)                                                                                                                                                                                                                     cGAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACCGCGACGGAAGCGATAGTgg                    >  1:533603/1‑251 (MQ=255)                                                                                                                                                                                                                      gAAGTGAGTTCCTTTAATCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATCCGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGCTAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACCGCGACGGAAGCGATAGTGGc                   >  2:297823/1‑251 (MQ=255)                                                                                                                                                                                                                               tCCTTTAATAAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGACGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACCGCGACGGAAGCGATAGTGGCTAATGGCa           >  1:274339/1‑250 (MQ=255)                                                                                                                                                                                                                                       tCAGCTTGTCGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACCGCGACGGAAGCGATAGTGGCTAATGGCAATCCccagc  >  1:551382/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  CGGAACAGCCTCTGATTTGTCAGCCTTTTTTGATTGCGGCGTAACGATAATGCAAACGATAACAATTATCAATCCAAATGTTAAATTTTATATCCGCATGATTTGTCAGGGAAATGGAATTGAAAAAGCCCTCTCGGTTGAGAGGGCTTAGCAAGGAAGGGAGGAAACTTATTTCTTATCGTTCTGCGGGAACTTCATTTCGCTGTAGCGTACGAAGTGAGTTCCTTTAATCAGCTTGTAGCCGAACCAAATAATCAGGAACAGCGGGATACCAATATACGTTGCCGCTACGCCGCCCCAGTCAATAGTATCTTTCAGGAACGCTTCGTAGTTCTGGCCCAAAGTGATAATCAGACACAGAATGAATGCGAAGATCGGCCCCAGTGGGAAGAAACCTGAACGGTACGGCAGATCGTTAATGTCGTGTCCCTGCAATACGTAACCGCGACGGAAGCGATAGTGGCTAATGGCAATCCCCAGC  >  NC_000913/2246895‑2247375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑