Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,173,448	C→T	intergenic (‑575/+834)	gatD ← / ← gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,173,448	0	C	T	100.0%	185.6 / NA	52	intergenic (‑575/+834)	gatD/gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/19);  total (33/19)

AAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGAT  >  NC_000913/2173207‑2173690                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   aaGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCAGCGCCACGAAGAAGCCGAGGGTGGCAAGATCGCAAAACGGCAGCACCTGATTACCCGGCACACAAACAGCATTTAAAATGGTGTGGGGGATAAAATT‑CAGGTTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGAt                                                                                                                                                                                                                                         <  1:552554/251‑1 (MQ=255)        ggAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTCGCGATGGGCGtt                                                                                                                                                                                                                                  >  2:389607/1‑251 (MQ=255)                 ttCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGAGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAAccatcc                                                                                                                                                                                                                         >  2:184473/1‑251 (MQ=255)                        gtcacgtggcccccaccgcGCCAGGAAGAAGCCAATGTGGGAAAGACCGCTAAACGGCCGCCCCAGATTCCCCGCCACACAAACAGCAATTAAAGTGGTGATGGGGAAAAAACT‑CGGGCTTGCCAATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATgg                                                                                                                                                                                                                  <  2:177325/233‑1 (MQ=255)                        aTGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGAATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAACCGCCTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATgg                                                                                                                                                                                                                  >  1:49109/1‑251 (MQ=255)                             cGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCAAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAG‑CAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTa                                                                                                                                                                                                             <  1:62532/251‑1 (MQ=255)                                 gACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGCGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATc                                                                                                                                                                                                          >  1:45497/1‑250 (MQ=255)                                  aCCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAc                                                                                                                                                                                                        >  2:95596/1‑251 (MQ=255)                                           gCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACATGATTACCCGGCCCACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAt                                                                                                                                                                                               >  2:261592/1‑251 (MQ=255)                                             cACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAtt                                                                                                                                                                                              >  2:186550/1‑250 (MQ=255)                                                    aagCCGATGGTGGCAAAATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCAt                                                                                                                                                                                      <  1:223002/251‑1 (MQ=255)                                                                 cAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAAAAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGtt                                                                                                                                                                         >  2:101382/1‑251 (MQ=255)                                                                               ggCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGAGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAACAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACAATCCATGATGGGTTTAATCACACGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTg                                                                                                                                                           >  1:289880/1‑251 (MQ=255)                                                                                  agcaCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCa                                                                                                                                                         >  2:527627/1‑250 (MQ=255)                                                                                                  gCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCg                                                                                                                                        >  2:187697/1‑251 (MQ=255)                                                                                                  gCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCg                                                                                                                                        >  1:528951/1‑251 (MQ=255)                                                                                                    acacaAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGt                                                                                                                                       >  2:112415/1‑250 (MQ=255)                                                                                                      acaAACAGCAGTTGCAATGGAGCGTGGGCTAAAACT‑CCGGCTTGCCGATACCACC‑‑GCAGTCTGTCCCAGCAGCAACGCAGGATAAAGGACAATCAGA‑CACTACTGACCGCCGAACTTCGCCTGTAAACGACTACAAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTCGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCACTAATCCTTTGACAtagaaac                                                                                                                                    >  2:263092/1‑245 (MQ=255)                                                                                                         aaCAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAAcccc                                                                                                                                 >  2:208546/1‑250 (MQ=255)                                                                                                          aCAGCAATTCAAATGGTGAGTGGGATAAACAT‑CAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCcgc                                                                                                                                >  2:245518/1‑251 (MQ=255)                                                                                                          aCAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCACC‑‑GCCGTATGGCCCAGCAGCAACGCCGGATCAAGTCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAACAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCcgc                                                                                                                                >  1:247153/1‑251 (MQ=255)                                                                                                           acaaaattgaaagtggtggggggAAAAAAATACAGTCTTCCCGATACCACC‑‑GCCGTATGCCCCAGAAGCACCGCCGGATCAAGGCCAATCAGG‑AACTCCGGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCCTCACTGCCGCCGTTTTTACCGCCAGCTTCAATACACCTTTGACATCGTAACCcgc                                                                                                                                <  2:451934/231‑1 (MQ=255)                                                                                                                     aaTGGAGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCACC‑‑GCCGTATGTACCAGCAGCAAAGCCGGATCAAGGCCACTCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTCAACGACTACGAGCTTGCTTCGCGATGGGCGGTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCACTACACCTTTGACATCGTAACCCGCGAGGATGCCg                                                                                                                      >  2:534139/1‑250 (MQ=255)                                                                                                                         gTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAAt                                                                                                                 <  1:150476/251‑1 (MQ=255)                                                                                                                            aGTGGGATAAAAAT‑CAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATagtc                                                                                                                >  2:108504/1‑246 (MQ=255)                                                                                                                                     aaaaT‑CAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATcac                                                                                                     >  1:460111/1‑251 (MQ=255)                                                                                                                                     aaaaT‑CAGACTTGCCGATACCACC‑‑GCCGTATTTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATTGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATcac                                                                                                     <  1:2505/251‑1 (MQ=255)                                                                                                                                     aaaaC‑CAGCCTTGCCAATACCACCC‑‑CCGTAGGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATcac                                                                                                     <  1:250727/251‑1 (MQ=255)                                                                                                                                      aaaaaaccaggcttcccgttcccaccgccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGT‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATc                                                                                                       <  2:427441/227‑1 (MQ=255)                                                                                                                                      aaaT‑CACGCTTGCCGATACCACC‑‑GCCGCATGTCCCAGCAGCAAAGCCGGATCACGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAAAGACTACGAGCTTGCTTAGCGATGGGCGTTATACCATCAATGCTGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGACGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCTGATAATCCGACCCATcac                                                                                                     >  2:357241/1‑250 (MQ=255)                                                                                                                                              gggcttgcctacacctccgccgTGTGTCCCAGCAGCAACGCCGGATCAAGGCCAA‑CAGGTAACTCCTTACCGCCGAATTTCGCCTGTAAACGACTCCGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGTTTTATTCGCCCTTGTCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCGTTGACATCGTAACCCGCGAGGATGCCGATAATCATCCCCATCACAAAAcc                                                                                               <  2:241257/235‑1 (MQ=255)                                                                                                                                                        atcccaccgccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACa                                                                                     <  1:10849/245‑1 (MQ=255)                                                                                                                                                        ataccaccgccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGAc                                                                                      >  2:160804/7‑250 (MQ=255)                                                                                                                                                         taccaccgccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGAc                                                                                      >  1:440064/6‑249 (MQ=255)                                                                                                                                                                gccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGcc                                                                             >  1:455401/1‑251 (MQ=255)                                                                                                                                                                         ccctgcagcaACACTGGATCAAGTCCATTCAGG‑AACTCCTGACCGCCGAACGTCGCCGTTAAACGCCTACGAGCTTGCTTAGCGATGGTCTTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGTAGCATCACTGCCGCCGTTTTTCCCGCCAGCTGCAATACACCTTTGACATCGTAACACGCGAGGATGCCGATAATCAGCCCCATCACAAACCCCACGTTGACAGTCTCGCCAAATGGAcc                                                                    <  1:296920/247‑1 (MQ=255)                                                                                                                                                                                  aaCGCCGGATCAAGGCCAATCAGG‑AACTCCTGCCCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTgcg                                                           <  2:171857/251‑1 (MQ=255)                                                                                                                                                                                  aaCGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTgcg                                                           <  1:95596/251‑1 (MQ=255)                                                                                                                                                                                             aaGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATAtcgt                                                <  1:8092/251‑1 (MQ=255)                                                                                                                                                                                                  cAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGgcg                                           >  1:454212/1‑251 (MQ=255)                                                                                                                                                                                                         g‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGAGCTGAATATCGTCGGCGCTAAAtt                                    >  1:403830/1‑251 (MQ=255)                                                                                                                                                                                                            ccTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCAGGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCTCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTa                                  <  2:225249/250‑1 (MQ=255)                                                                                                                                                                                                              tCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAAt                                >  1:368036/1‑251 (MQ=255)                                                                                                                                                                                                                 tGACCGCAGCACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAAAAATCCATGATGGGTTTAATCACCCGTGGCATTCGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGACCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTCAATTTAATTCg                             >  2:492064/1‑251 (MQ=255)                                                                                                                                                                                                                  gACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCgg                            >  1:65077/1‑251 (MQ=255)                                                                                                                                                                                                                        cgcaCTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGGTGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACg                      >  2:374976/4‑251 (MQ=255)                                                                                                                                                                                                                         gAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGc                     >  2:96476/1‑251 (MQ=255)                                                                                                                                                                                                                          aaCTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGcc                    <  2:440064/251‑1 (MQ=255)                                                                                                                                                                                                                                 ccTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGAtt             <  1:405250/251‑1 (MQ=255)                                                                                                                                                                                                                                      aaaCGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTtctc        <  1:112415/251‑1 (MQ=255)                                                                                                                                                                                                                                            cTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCgatgat  >  2:209788/1‑251 (MQ=255)                                                                                                                                                                                                                                            cTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCgatgat  >  1:516326/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   AAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGG‑AACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGAT  >  NC_000913/2173207‑2173690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑