Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,104,024	G→T	I162I (ATC→ATA)	wbbK ←	putative glycosyltransferase WbbK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,104,024	0	G	T	100.0%	21.1 / NA	7	I162I (ATC→ATA)	wbbK	putative glycosyltransferase WbbK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (4/3);  total (4/3)

CCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATT  >  NC_000913/2103800‑2104257                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                          ccACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTTATAAATTTTTCTTTCATCCAGAAttg                                                                                                                                                                                                                 >  2:348736/1‑251 (MQ=255)                                                                    gCTCGTAATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTTATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAAt                                                                                                                                              >  1:493192/1‑251 (MQ=255)                                                                                                                                                  catcatcaGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTTATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATAcaca                                                                <  1:348736/251‑1 (MQ=255)                                                                                                                                                                                atttaatttCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTTATAAATTTTTCTTTCATCCAGa                                                                                                                                                                                                                     <  1:29791/72‑1 (MQ=255)                                                                                                                                                                                atttaatttCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTTATAAATTTTTCTTTCATCCAGa                                                                                                                                                                                                                     >  2:29791/1‑72 (MQ=255)                                                                                                                                                                                                      attgatgTTATTTATCGCATATTTCTTTATAACTTTTTCTTTAATCCAGTATTGTTTAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATTAGCCCGTATATAATTTTAAATAAGAAAAAGCTAAGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATTCAGAcaaatcaaat            >  2:311015/3‑251 (MQ=255)                                                                                                                                                                                                                 ttatAGAATATTTCTTTATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCAtt  <  2:493192/250‑1 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                          CCACTGATAGTAAGCAGAAATTTAATATTGGATTGTTCTTTCAATTTCCTTGCTGCACTAATAATAAGCTCGTAATTTTTAAATACTCGTGGAACAGCAGGGTAAAATATTGTCAACTCAGAAGGGTTATTCTTAAATTGAGAATCATCATCAGTAAGTTGGCTTTTATCAGATAATTTAATTTCTGGCCGACTGACAATGATGTTATTTATAGAATATTTCTTGATAAATTTTTCTTTCATCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATT  >  NC_000913/2103800‑2104257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑