Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	309,381	C→T	D216N (GAT→AAT)	ecpB ←	putative fimbrial chaperone EcpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	309,381	0	C	T	100.0%	238.5 / NA	66	D216N (GAT→AAT)	ecpB	putative fimbrial chaperone EcpB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/31);  total (35/31)

CCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGAGCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAATGGTGCCGATGCGGGCG  >  NC_000913/309134‑309625                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     ccTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTAcc                                                                                                                                                                                                                                                   <  1:103995/251‑1 (MQ=255)          cGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCaa                                                                                                                                                                                                                                            >  1:9011/1‑249 (MQ=255)             cGAGATGAATATAGCGCGGGACGCTCATGCCGTCCTGAAGCGACTAACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACCAAAAGACCATGCCGAAGGCAAACTGCGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGTAATGAACTTATTACCCTGCCAAAGTGc                                                                                                                                                                                                                                       >  2:89663/1‑251 (MQ=255)                gaTGAATCTAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGc                                                                                                                                                                                                                                               >  2:65511/1‑240 (MQ=255)                gaTGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGc                                                                                                                                                                                                                                               <  1:65511/240‑1 (MQ=255)                     atatAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCGGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCAGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAGCTGGGCTGTCAGGCCTGGGGAGAACCGTCGTAAAGGCATGGTCGGTATCCATCTGCACAGCTAGCTAGTTCATGGGAATGAACTTATTTCCCCGCTAAACCGCAACCCGTc                                                                                                                                                                                                                               <  2:234558/251‑1 (MQ=255)                         agagCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCtt                                                                                                                                                                                                                            <  1:275537/250‑1 (MQ=255)                           agCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCAGTCCTTTGt                                                                                                                                                                                                                         >  2:178417/1‑251 (MQ=255)                              gggACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTAt                                                                                                                                                                                                                      >  1:430000/1‑251 (MQ=255)                                gACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATcc                                                                                                                                                                                                                    <  1:267052/251‑1 (MQ=255)                                      cATGCCGTCCTGAAGCGCCTGACTTAAGTCCTGCTGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACATCTATCTATTTCACGGTAATGAACTTATTACCCTGCCAAAGTGCAACCCGGCCTTTGTTATCCGCAgt                                                                                                                                                                                                               <  1:364940/250‑1 (MQ=255)                                         gCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTTGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTc                                                                                                                                                                                                            <  1:319227/250‑1 (MQ=255)                                           cGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCAc                                                                                                                                                                                                         >  2:390112/1‑251 (MQ=255)                                                 gAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCgg                                                                                                                                                                                                     <  2:410596/249‑1 (MQ=255)                                                 gAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGt                                                                                                                                                                                                   <  1:525674/251‑1 (MQ=255)                                                       ccTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAacg                                                                                                                                                                                             >  2:428204/1‑251 (MQ=255)                                                             tgaAGCCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGACAGCAGCGCTGGCATCGGGCTGAACGACCAAAAAGCCCATGCCGAAGGCACACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGAcgcg                                                                                                                                                                                        >  2:502603/1‑250 (MQ=255)                                                              gaAGGCCTGCGGAATAATCACCCCCCCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGAc                                                                                                                                                                                      <  1:508919/251‑1 (MQ=255)                                                               aaGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACt                                                                                                                                                                                     <  1:361701/251‑1 (MQ=255)                                                               aaGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACt                                                                                                                                                                                     <  2:126044/251‑1 (MQ=255)                                                                     tGCGGAATAATCAACCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGGCCTTTGTTATCCGCCGTGTCCACGCGGGTAAAACGACGCGACTTGCCCg                                                                                                                                                                               >  2:457240/1‑251 (MQ=255)                                                                         gAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACCGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCAt                                                                                                                                                                           >  2:420023/1‑251 (MQ=255)                                                                         gAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCAt                                                                                                                                                                           <  1:287412/251‑1 (MQ=255)                                                                           ataatCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACtt                                                                                                                                                                                    <  1:85519/240‑1 (MQ=255)                                                                           ataatCACCCCACATATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAACAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGACCCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACtt                                                                                                                                                                                    >  2:85519/1‑240 (MQ=255)                                                                              atCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGgt                                                                                                                                                                      <  2:455486/251‑1 (MQ=255)                                                                                cACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGgta                                                                                                                                                                     <  1:169062/250‑1 (MQ=255)                                                                                cACCCCACCGATTTGCTGCGCACGTATGTCAGCATCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACGGGGCTTGCAGTCCTGGTGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACTCGAATGTACTTATTACCCTGCCAAAGGGCAGCCCGTCCTTTGGTTTCCGCAGTGTCCACGCGGGTAAAACGTCGCGACTTGCGCCGCAACAGgtag                                                                                                                                                                    <  2:532617/251‑1 (MQ=255)                                                                                  ccccACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCACGGCATCAGgtagt                                                                                                                                                                   >  1:45852/1‑250 (MQ=255)                                                                                  ccccACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTGCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGgtagta                                                                                                                                                                  <  2:344982/251‑1 (MQ=255)                                                                                         tATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTGTCAGTCCTGGGGAGCACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCATGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTctct                                                                                                                                                           >  1:60124/1‑251 (MQ=255)                                                                                               ctgcGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGCACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTacac                                                                                                                                                     >  2:375951/1‑251 (MQ=255)                                                                                                tgcGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACt                                                                                                                                                    >  1:412407/1‑251 (MQ=255)                                                                                                    cACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCtt                                                                                                                                                >  1:424082/1‑251 (MQ=255)                                                                                                     aCTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAACCTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCtt                                                                                                                                                >  2:491485/1‑250 (MQ=255)                                                                                                        tATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAACAAGACCATGCCGAAGGCACACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAACAGCATTGTCGTTATCCATCTACCCAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCAGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGAAGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCg                                                                                                                                            >  2:161832/1‑251 (MQ=255)                                                                                                              agcagcGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTggcg                                                                                                                                      <  1:158892/251‑1 (MQ=255)                                                                                                                cagcGCTGGCATCGGGCTGAACGACCAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTggcggc                                                                                                                                    >  2:381494/1‑251 (MQ=255)                                                                                                                 agcGCTGGCATCGGGCTGAACGCACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCt                                                                                                                                   >  1:128739/1‑251 (MQ=255)                                                                                                                 agcGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCt                                                                                                                                   >  1:384094/1‑251 (MQ=255)                                                                                                                    gcTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTc                                                                                                                                >  1:118987/1‑251 (MQ=255)                                                                                                                    gcTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTc                                                                                                                                <  1:141356/251‑1 (MQ=255)                                                                                                                       ggCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAg                                                                                                                              <  2:471126/250‑1 (MQ=255)                                                                                                                                cTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCg                                                                                                                    >  1:481432/1‑251 (MQ=255)                                                                                                                                        acAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGGCCTGGGGAGAACCGGCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAg                                                                                                            <  1:420023/251‑1 (MQ=255)                                                                                                                                                cccATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGTGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGAGGGTAAAACGACGCGACTTGTCCGGCATCAGGTAGTAATTCTCTGTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGGTCCGCa                                                                                                    <  2:210994/250‑1 (MQ=255)                                                                                                                                                aCCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGc                                                                                                     >  2:277844/1‑250 (MQ=255)                                                                                                                                                     gCCGAAGGCAAACTGTGCTTTCAGTCCTTGGGAGAACCGTCGTAAAGGCATTGTCTTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTTCCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTc                                                                                               <  1:178417/251‑1 (MQ=255)                                                                                                                                                       cGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGc                                                                                             >  1:422729/1‑251 (MQ=255)                                                                                                                                                          aGGCAAACTGGGCTTACAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTCCCCTGCCAAAGTGCAACCCGGCCTTGGTTATCCGCATTGTCCACGCGGGTAAAACGTCGCGACTTGCCCGGCATCAGGTAGTAAGTCTCTTTACACTCCTTACCGTTGTCGGCTTTCAGGCAAGGGCCGTAGGCGACGATCCGCAGCGTCGCAtt                                                                                          <  2:248123/251‑1 (MQ=255)                                                                                                                                                                   gggCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGAATTGTCGTTATCCTTCTGCACAGCTCTCTATTTCCCGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTAtt                                                                                 >  2:517355/1‑251 (MQ=255)                                                                                                                                                                           aGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACCGCTATCTATTTCACGGGAATGACCTTATTACCCTGCCAAAGAGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAAATCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAggg                                                                         >  2:201415/1‑251 (MQ=255)                                                                                                                                                                            gTCCTGGGGAGAACCGTCGTAAATGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGa                                                                        <  2:136437/251‑1 (MQ=255)                                                                                                                                                                               cTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGCCTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGAGcc                                                                     >  2:192569/1‑251 (MQ=255)                                                                                                                                                                                 ggggCGAACCGTCGTAAATGCATTGTCGTTATCCTTCTGCACATCTATCGTTTTCACGGGAATGAACTTATTCCCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGAGTCCACGCGGGTAAAACGACGCGACTTGCCGGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGTTTTCAGGCAAGGGGCTTAGGCGAGGATCCGCAGCGGCGCATTTCCTGTATGGGTCAGGGAGCCGt                                                                   <  2:319861/251‑1 (MQ=255)                                                                                                                                                                                 ggggAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGAGCCg                                                                    <  1:154266/250‑1 (MQ=255)                                                                                                                                                                                    gaaaaCCGTCGTAAAGGCATTGTCGTTATCCAGCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGAGCCGTTgg                                                                <  2:379052/248‑1 (MQ=255)                                                                                                                                                                                        aCCGTCGTAAAGGCATGGTCGTTATCCATCTGCACAGCTATCTATTGCACGGGAATTAACTTATTACCCTGCCAAAGGGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACTACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTGACCGTTGTCGGCTTTCAGGCAAGTTCCGTAGGCGAGGATCCGCAGCGGCGCATTGCCTGTATTTGTCAGGGAGCCGTTGGCGTa                                                            <  2:536706/251‑1 (MQ=255)                                                                                                                                                                                         ccGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGCAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCCTTTCCTGTATTTGTCAGGGAGCCGTTGGCGTAc                                                           >  2:74253/1‑251 (MQ=255)                                                                                                                                                                                              gtAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGAGCCGTTGGCGTACTGaa                                                       >  1:258828/1‑250 (MQ=255)                                                                                                                                                                                                  aGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGAGCCGTTGGCGTACTGAAAGTgg                                                  <  1:433722/251‑1 (MQ=255)                                                                                                                                                                                                        tGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGAGCCGTTGGCGTACTGAAAGTGGTAGTTc                                            >  1:377690/1‑251 (MQ=255)                                                                                                                                                                                                                                        aCGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGCTCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGAGCCGTTGGCGTACTGCAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAATGGTGcc            >  2:122424/1‑251 (MQ=255)                                                                                                                                                                                                                                        aCGGGAATGAACTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGAGCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAATGGTGcc            >  1:86869/1‑251 (MQ=255)                                                                                                                                                                                                                                          gggcaTGACCTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAACACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCCTTTCCTGTATTTGTCCGGGAGCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAATGGTGCCgg          >  2:278495/5‑250 (MQ=255)                                                                                                                                                                                                                                                  aCTTATTACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGAGCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAATGGTGCCGATgcgggcg  >  1:455230/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     CCTGGCTACCGGCGAGATGAATATAGAGCGGGACGCTCATGCCGTCCTGAAGCGCCTGACTGAAGGCCTGCGGAATAATCACCCCACCTATTTGCTGCGCACTTATGTCAGCAGCGCTGGCATCGGGCTGAACGAACAAAAAGACCATGCCGAAGGCAAACTGGGCTTTCAGTCCTGGGGAGAACCGTCGTAAAGGCATTGTCGTTATCCATCTGCACAGCTATCTATTTCACGGGAATGAACTTATCACCCTGCCAAAGTGCAACCCGTCCTTTGTTATCCGCAGTGTCCACGCGGGTAAAACGACGCGACTTGCCCGGCATCAGGTAGTAATTCTCTTTACACTCCTTACCGTTGGCGGCTTTCAGGCAAGGTCCGTAGGCGAGGATCCGCAGCGTCGCATTTCCTGTATTTGTCAGGGAGCCGTTGGCGTACTGAAAGTGGTAGTTCGCCTGACGAGGGGCGACGACCAGAATGGTGCCGATGCGGGCG  >  NC_000913/309134‑309625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑