Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,645	G→A	W282* (TGG→TAG)	thrB →	homoserine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,645	0	G	A	100.0%	215.3 / NA	62	W282* (TGG→TAG)	thrB	homoserine kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/50);  total (12/50)

GCATTGCGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAG‑CTTT‑GCGCAAGCCGTAA‑CCCAGGGG‑TTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTG  >  NC_000913/3399‑3874                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           gcattgcGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTg                                                                                                                                                                                                                                        <  2:57022/251‑1 (MQ=255) gcattgcGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAACGAGTTGCCGCCTAGTTg                                                                                                                                                                                                                                        >  1:287947/1‑251 (MQ=255)      gcgcACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTaa                                                                                                                                                                                                                                   <  1:79730/251‑1 (MQ=255)        gcACGGGGGACATCGGGCGGGTTTCATTCCGGCCTGGTCGTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGGGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGGAGCGAGCGGTATCTTCGGCTCCGGCCCGACCTGGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGTGTTGCCGACTAGTTGGGTAAGa                                                                                                                                                                                                                                 <  2:518474/251‑1 (MQ=255)           cGGGCGACGTCTGGGAGGCTTCATGCAGGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCGGATGAAAGATGTTAGCGCTGAACCCTACCGGTAACGGTTACCGCCAGGCTTCCGGCATGTGCGGCAGGCGGTCGCGGAAATCGTCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGCCCTTCTTCGCGCTGTGTGACAAGCCGGAAACCGTCCAGCGCGTTGCCGACTAGTTGGGTAAGaa                                                                                                                                                                                                                                <  1:35438/249‑1 (MQ=255)            gggCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACt                                                                                                                                                                                                                              >  1:121093/1‑250 (MQ=255)                gACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCCGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTg                                                                                                                                                                                                                         <  1:363657/251‑1 (MQ=255)                       tgcatgcgtcattcaCGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGTAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGAACAGCGTTTTGCCGACTAGTTGGGTAAGAACTACCTGCaaaa                                                                                                                                                                                                                    <  1:157650/241‑1 (MQ=255)                       ggCAGGCTGCAGTCTCGTCTGCGAGTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTGACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAAGCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTGCGCTCTGTGTGACAAGCCGGAAACCGACCAGCGCGTTGCCGACTATTTGGGTAAGAACTACCTGCaaaa                                                                                                                                                                                                                    <  2:469353/249‑1 (MQ=255)                        gccggCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTAGCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGCTGGGTAAGAACTACCTGCAAAATCa                                                                                                                                                                                                                 <  2:98944/248‑1 (MQ=255)                            gCTTCATTCACGCCAGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTAGCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCGCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTATTTGGGTAAGAACTACCTGCAAAAGCAGGaa                                                                                                                                                                                                             <  2:78316/251‑1 (MQ=255)                                  ttcaCGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGCAACCGCCCCGCGCGTTGCCGCCTAGTTGGGTAAGAACTACCTGCAACATCAGGacgggttt                                                                                                                                                                                                       >  2:295826/1‑244 (MQ=255)                                    caCGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGt                                                                                                                                                                                                     <  1:260492/251‑1 (MQ=255)                                        ccTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAACCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCCAAATCAGGAAGGTTTTGTTCat                                                                                                                                                                                                 >  1:349281/1‑251 (MQ=255)                                           gCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCata                                                                                                                                                                                                <  2:188843/249‑1 (MQ=255)                                              agtCCCGTCAGCCTGAGCTTGCCTCGAAGCTGATGAAAGATGTTAGCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTTTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATAttt                                                                                                                                                                                             <  2:70669/247‑1 (MQ=255)                                                 cccGTCAGCCTTATCTTGACGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCg                                                                                                                                                                                         <  2:294114/250‑1 (MQ=255)                                                 cccGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCgg                                                                                                                                                                                        <  2:358345/251‑1 (MQ=255)                                                      cAGCCTGAGCTTGACGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGGATCTCCGGCTCCGGCCCGCCCTTGGTCGCTCTGTGTGACAAGACGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGACAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTggg                                                                                                                                                                                   >  2:43527/1‑250 (MQ=255)                                                           tGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATAcgg                                                                                                                                                                              >  2:535593/1‑251 (MQ=255)                                                                 tGCCGCTAAGCTGATGAAAGAGGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCGTCCGGGAGGCGCGGCAGGCGGGCGCGGAAATCGGCGCGGTAGCGAGCGGTATCGCCGGCTCCGGCCCGACCTTGTGCGCGCTGTGTGACAAGCCGGAAATCGGCCGGCGCGTTGCCGGCTAGTTGGGGAAGAACTTCCTGCAGAAGCAGGTAGGTTTTGATCATATTTGCCGGCTGGACGCggcgggc                                                                                                                                                                         <  2:525148/250‑1 (MQ=255)                                                                 tGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACggcgggc                                                                                                                                                                         <  1:414068/250‑1 (MQ=255)                                                                             gatgaAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTgg                                                                                                                                                           <  1:277001/251‑1 (MQ=255)                                                                             gatgaAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTgg                                                                                                                                                           <  1:266120/251‑1 (MQ=255)                                                                              atgaAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGa                                                                                                                                                          <  1:237696/251‑1 (MQ=255)                                                                                 aaaGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACggcggggcggacgggacatgtaa                                                                                                                                                         >  1:309957/1‑233 (MQ=255)                                                                                          aTCGCTGAACCCTACCGTGAACTGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGTCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGaa                                                                                                                                              <  1:207836/251‑1 (MQ=255)                                                                                            cGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAAc                                                                                                                                            <  2:176077/251‑1 (MQ=255)                                                                                             gCTGAACCCGACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCTGCTGGATCCGGCGGGCGCACGAG‑TACTGGAAAGCTAAATGAAAct                                                                                                                                           <  2:375111/251‑1 (MQ=255)                                                                                             gCTGAACCATACCGGGAACGGTTACTGCCAGGCTGCCGGCAGGGCCGGCTGGCGTGCGCGTAAATGGGCGCGGACGCGAGCGGTATCTCCGGCTCCGGCCCCCCCTTTGTCGCTCTGTGTGACAAGCCGGAAACCTCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAGGGTTTGGTGCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGTACAACTAAATGAAAct                                                                                                                                           <  2:46648/251‑1 (MQ=255)                                                                                                gAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACTCTa                                                                                                                                        <  2:25242/251‑1 (MQ=255)                                                                                                   cccTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAATCTAAATGAAACTCTACaa                                                                                                                                     <  1:353011/251‑1 (MQ=255)                                                                                                   cccTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCGGCAAAATCAGGAAGGGTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACTCTACaa                                                                                                                                     <  1:277184/251‑1 (MQ=255)                                                                                                             aaCGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGTCTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCCAGAG‑TACTGGAAAAATAAACTAAAAACTACAATCtaaa                                                                                                                               >  1:157177/1‑244 (MQ=255)                                                                                                                   tACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACaa                                                                                                                      <  1:235431/250‑1 (MQ=255)                                                                                                                    aCTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAAc                                                                                                                     <  1:408904/250‑1 (MQ=255)                                                                                                                    aCTGCCAGGCGTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTAGCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACTCTACAAGCTGAAAGATCACAAc                                                                                                                     <  2:198108/250‑1 (MQ=255)                                                                                                                       gCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGAAAATCGGCGCGGTAGCGAGCTGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGa                                                                                                                   <  1:386164/249‑1 (MQ=255)                                                                                                                                   gtgggcgcggcaggcgGGCGCGGAAATCGGCGGGGTAGCGAGCGGTATCTCCGGCGTCGGCCGGACCTTGTTCGGTATGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTGTTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACGGGAAATCTAAATGAAACTCTACGATCTGAAAGATCACAGCGAGCAGGTCAG‑Ctttt                                                                                                    <  2:96838/248‑2 (MQ=255)                                                                                                                                          cggcaggcgGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAG‑CTTT‑GCGCAAGc                                                                                            <  2:121093/251‑1 (MQ=255)                                                                                                                                             caggcgGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAATGTTTTGTTCATATTTGCCGGCTGGATACGTCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAG‑CTTT‑GCGCAAGCCGt                                                                                         <  1:245198/251‑1 (MQ=255)                                                                                                                                             caggcgGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAG‑CTTT‑GCGCAATCCGt                                                                                         <  2:361267/251‑1 (MQ=255)                                                                                                                                                          aaaTCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑GACTGGGAAAATAAAATAAACTCTACAATCTGAAAAATCACCACCAGCAGGGCAG‑CTTT‑TCGCAAGCCgcaaaccacggggtg                                                                            >  1:250516/1‑237 (MQ=255)                                                                                                                                                                       agcgagcgGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAG‑CTTT‑GCGCAAGCCGTAA‑CCCAGGGG‑TTGGGCAAAAATCAg                                                              <  1:228290/250‑1 (MQ=255)                                                                                                                                                                         cgagcgGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGGGCACGGGGAATTGGAAAA‑AAAATGAAAATCTAAAAACTGAAAGATAACAACGAGCAGGGCAG‑CTTT‑TGGCAAGGAGTAAACCCAGGGG‑G‑GGGGAAAAAACAgggg                                                           >  1:215792/1‑251 (MQ=255)                                                                                                                                                                          gagcgGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAG‑CTTT‑GCGCAAGCCGTAA‑CCCAGGGG‑TTGGGCAAAAATCAGGGGc                                                          <  1:374305/251‑1 (MQ=255)                                                                                                                                                                           ggctgTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAG‑CTTT‑GCGCAAGCCGTAA‑CCCAGGGG‑TTGGGCAAAAATCAGGGGCt                                                         <  1:405052/247‑1 (MQ=255)                                                                                                                                                                            gcgGTAGCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAG‑CTTT‑GCGCAAGCCGTAA‑CCCAGGGG‑TTGGGCAAAAATCAGGGGCTg                                                        <  1:478612/251‑1 (MQ=255)                                                                                                                                                                                   ctccggctccggCCCGCCCTTGTTCGCTCGGTGTGACAAGCCGGAAACCGCCCAGTGCGTTGCCGACTAGTTGGTTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACGCTACAATCTGAAAGTGCACAACGAGCAGGTCAG‑CTTT‑GCGCAAGCCGTAA‑CCCAGGGG‑TTGGGCAAAAATCAGGGTCTGttttt                                                   <  2:251635/249‑1 (MQ=255)                                                                                                                                                                                   ctccggctccggCCCGACCGTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACGCTACAATCTGAAAGATCACAACGAGCAGGTCAG‑CTTT‑GCGCAAGCCGTAA‑CCCAGGGG‑TTGGGCAAAAATCAGGGGCTGttttt                                                   <  2:287947/249‑1 (MQ=255)                                                                                                                                                                                       ggctccggcCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGCG‑GAATGGAAAACTAAATTAAACTCTTCAATCTGTAAAATTACAACGAACCGGTTAGACTTTTGC‑CAA‑CCGTAA‑CCCAGGGGGTTGGGCaaaatgagggggtggttttttcgcc                                              >  1:364442/1‑230 (MQ=255)                                                                                                                                                                                           ccggcCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAG‑CTTT‑GCGCAAGCCGTAA‑CCCAGGTG‑TTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACg                                           <  1:140209/249‑1 (MQ=255)                                                                                                                                                                                           ccggcCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAG‑CTTT‑GCGCAAGCCGTAA‑CCCAGGGG‑TTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACg                                           <  2:460657/249‑1 (MQ=255)                                                                                                                                                                                              gcCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAATCTCTACAATCTGAAAGATCACAACGAGCAGGTCAG‑CTTT‑GCGCAAGCCGTAA‑CCCAGGGG‑TTGGTCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTg                                      <  1:214204/251‑1 (MQ=255)                                                                                                                                                                                                              tctGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAG‑CTTT‑GCGCAAGCCGTAA‑CCCAGGGG‑TTGGGCAAAAATCAGGGTCTGTTTTTTCCGCACGACCTGCCGGAATTCa                            <  2:72595/245‑1 (MQ=255)                                                                                                                                                                                                              tctGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑GAATGGGAAAATAAATGTAAAACTACACAATGAAAGATCACCACGAGCAGGTCCG‑CTTT‑TCGCAAGCCGGAA‑CCCAAGGG‑TGGGGGAAAAAAAAGGGGGTGGTTTTTTCGCCCGGACTGCCGGAGTTct                            >  1:72595/1‑244 (MQ=255)                                                                                                                                                                                                               ctGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCTGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTGGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAG‑CTTT‑GCGCAAGCCGTAA‑CCCAGGGG‑TTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCctg                       <  2:530411/249‑1 (MQ=255)                                                                                                                                                                                                                 gtgtgACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAG‑CTTT‑GCGCAAGCCGTAA‑CCCAGGGG‑TTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCctgact                    <  1:13978/250‑1 (MQ=255)                                                                                                                                                                                                                     gACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAG‑CTTT‑GCGCAAGCCGTAA‑CCCAGGGG‑TTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGaaa                <  1:535593/250‑1 (MQ=255)                                                                                                                                                                                                                         aGCCGGAAACCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAG‑CTTT‑GCGCAAGCCGTAA‑CCCAGGGG‑TTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATtgat           <  2:105839/251‑1 (MQ=255)                                                                                                                                                                                                                                 aCCGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAG‑CTTT‑GCGCAAGCCGTAA‑CCCAGGGG‑TTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCt   <  2:439833/251‑1 (MQ=255)                                                                                                                                                                                                                                  ccGCCCAGCGCGTTGCCGACTAGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACGCTACAAGCTGAAAGATCACAACGAGCAGGTCAG‑CTTT‑GCGCAAGCCGTAA‑CCCAGGGG‑TTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTg  <  1:493072/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                           GCATTGCGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAG‑TACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAG‑CTTT‑GCGCAAGCCGTAA‑CCCAGGGG‑TTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTGAAATTGATGAGATGCTG  >  NC_000913/3399‑3874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑