Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,782,9080TG37.2% 29.3 / 15.1 58T252P (ACC→CCC) gpsAglycerol‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (10/22);  new base G (18/1);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 7.18e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑GTTGTCGGTACAGGTAAGCACCAGAT‑CGCCAAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCAGCGCCGTACGCGCA‑TTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAG  >  NC_000913/3782667‑3783151
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                         
cGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑GTTGTTGGGACAGGGAAGCAcc                                                                                                                                                                                                                                                    >  1:282940/1‑249 (MQ=255)
    cACGACCTCGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGTGTT‑TCGGGACAGGGAAGCACCAgctac                                                                                                                                                                                                                                              >  1:201509/1‑247 (MQ=255)
                  gTCAATGCTGCCTCGCGCGGGTTTTTTCCGCAATATAATACTTGAAAAATTCCCTCGTTTATTGGCATTTCAACGCCGAGGCGGTGCGCCAGTTCGCGGACTTCTTTCGTATGGCGGTACCCTTCCACCCCCTGACCAA‑TCTTCT‑C‑CTG‑CGCGTTTTGTACACCCATGCCCTGACCGAGCATCATGCCAAAACGGCGTTTACGCGACTG‑GTTGTCGGTACAGGTAAGCCCCAGAT‑CGCCAAGCCCCGCCAt                                                                                                                                                                                                                               <  2:232036/251‑1 (MQ=255)
                     aaTGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGGCTG‑GGTGTAGGTACAGGTAAGAAACAGAT‑AGCCAAGCCCCGCCATGcc                                                                                                                                                                                                                            >  1:385845/1‑251 (MQ=255)
                               cgcgcgcgTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCACCGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGCCTG‑GTTGGCGGTACAGGTAAGCACCAGAT‑CGCCAAGCCCCGCCATGCCCATAAAGGt                                                                                                                                                                                                                   <  1:47393/250‑1 (MQ=255)
                                    gcgTTTTTGCCGCAATATAATCCTGGATAATTTTCCGCGGTTATTGGCTTTTCAACGCCGAATCGATGCGCCAGTTCTCGGACTTCTTTCTTAGTGCGGTAGCCTTCCTCCCCCTG‑CCAACTCTTCT‑C‑CTG‑CTCGCTTTGTACATCCATGCCCTGACCGACCACCATGCCCAAACGTCGGTTACGCGACTG‑GTTGTCGGTAAAGGTAAGCCCCAAAT‑CGCCTAGCCCCGCCATGCCCATAAAGGTGGC‑Agg                                                                                                                                                                                                            <  1:387707/251‑1 (MQ=255)
                                            tCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTCGCGACTG‑GTTGTCGGGACAGGTAAGCACCAGAT‑CGCCAAGCCCCCCCATGCCCCTAAAGGGGGC‑AGGGGCGGCAc                                                                                                                                                                                                    >  1:233258/1‑251 (MQ=255)
                                              cGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTCACGGCTG‑GGTGTCGGAGCAGGAACGCACCCGCT‑CGCCCAGCCCCCACATGCCCATCAAAGCGGC‑AAGGGCGGCAccc                                                                                                                                                                                                  >  1:334262/1‑251 (MQ=255)
                                                 aaTATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAAAGGCGGCTAAGCGGCCG‑GGTGTGGGGACAGGGAAGCACCAAAA‑CCCCAAACCCCGCCAACCCCATAAAGGGGGG‑AGGGGCGGCaacccac                                                                                                                                                                                               >  1:497311/1‑245 (MQ=255)
                                                 aaTATAATACTTGATAAATTGCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCCCCCCCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑GTTGGCGGTACAGGTAAGCACCAGAT‑CGCCAAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAgc                                                                                                                                                                                               <  1:258562/251‑1 (MQ=255)
                                                  atatAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGCCTG‑GTTGTCGGTACAGGGAAGCACCAGAT‑TGCCCAACCCCGCCATGCCCATAAAGGTgggaagggcggccacccacc                                                                                                                                                                                               >  1:165472/1‑233 (MQ=255)
                                                   tataATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACCG‑GGTGTCGGTAAAAGGAAGCACCAGAT‑CGGCAAGCGCCGCCAAGGCCATAAAAGTGGG‑AGGGTCGGCACCCAac‑gc                                                                                                                                                                                            >  1:220155/1‑251 (MQ=255)
                                                         aCTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGAGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTAAGCGACTG‑GATGTCGGTAAAGGTAAGCACCCGAT‑CGCCAACCCCCCCCCTGCCCCTAAAGGGGGC‑AGGGGCGGCAACCAGC‑GCCGCAcc                                                                                                                                                                                      >  1:33489/1‑251 (MQ=255)
                                                          cTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGGCTG‑GTTGTCGGTACAGGGAAGCACCAGAT‑CGCCAAACCCCCCCCTGCCCATAAAGGGGGC‑AGGGTCGGCAccccccgcccccccc                                                                                                                                                                                      >  1:136759/1‑239 (MQ=255)
                                                                  aTTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACACGACTG‑GTTGTCGGGACAGGTAAGCAAAAGAT‑CGCCAACCCCCGCCAACCCCATAAAGGCGGG‑CGGGGCGGCAACCAGC‑GCCGCACCCAAGAccg                                                                                                                                                                              >  1:18637/1‑248 (MQ=255)
                                                                        ccGGTTATTGGCATTTCAACGCCGAAGCGAGGCTCCAGTCCGCGGACTTCTTTCTTATTGCGGTACCCTCCCCCCCCCGGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑GTTGTCGGTACATGTAAGCACCAGAT‑CGCCAAGCCCCGCCATGCCCATAAATGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTc                                                                                                                                                                      <  2:320893/250‑1 (MQ=255)
                                                                         cgtttatggcgaTTTCAACGCCGAGCCGATGCGCCACTTCGCGGAGTTCTTTCGTATGCCGGTAGCTTTCCCCCCCCGCTCCAA‑‑CCTCTGC‑CTG‑CGCGCTTTGTACATCCATGCCCTGCCGGAGCATCATGCCAAAACGGCGGTTTCGCGACTG‑GTGGTCGGTACAGGTAAGCTCGAGAG‑CGCGTAGCCCCGCCATGTCCAGATAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTc                                                                                                                                                                      <  1:259075/239‑1 (MQ=255)
                                                                         cGGTTGTTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGATTTTTTCCGTATTGCGGTAGCCTCCCACCCCCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCAGGCCAAAACGGCGGTTCCGCGACTG‑GTTGTCGGTACAGGTAAGCACCAGAT‑CGCCAAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTc                                                                                                                                                                      <  2:145195/250‑1 (MQ=255)
                                                                          ggTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCCAAACGGCGGTTACGCGCCTG‑GGTGTAGGGAAAGGGAAGCACCAAAT‑AGCCCAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGGCGGCACCCCGC‑GCCGCACC‑CAAACGCGACATTTca                                                                                                                                                                     >  1:79973/1‑250 (MQ=255)
                                                                           gTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACCG‑AGTGTCGGGAAAGGGAAGCACCAGAT‑CGCCCAACCCCACCACGCCCCTAAAAGAGGG‑AGGGTAGGCACCCAGA‑GCCACACC‑aaaccgcgcaatttctac                                                                                                                                                                   >  1:378311/1‑235 (MQ=255)
                                                                               ttGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGAATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTAACGCGACTG‑GGTGTCGGGACAGGTAAGGACCAAAT‑CGCCCAACCCCCCCACGCCCACAAAAGTGGC‑AGGGGCGGCACCCAGC‑GCCGCACC‑ACAACGCGGCAttttcaccaac                                                                                                                                                               >  1:437254/1‑243 (MQ=255)
                                                                                  gCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTCCCCGCCTG‑GTTGTCGGTACAGGACAGCCCCCAAA‑CGCCAAGCCCCGCCATGCCCCTAAAGGTGGC‑AGGGGCGGCACCCCGC‑GCCGCCCC‑CAGCCCCGACATTTCAgcccgcccc                                                                                                                                                            >  2:476256/1‑250 (MQ=255)
                                                                                    aTTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACCCGACTG‑GGTGTCGGGACAGGGAAGCACCAGAA‑CGCCCAGCCCCCCCCAGCCCCTAAAGGGGGG‑CGGGGCGGGCCCCCGC‑GCCGCACC‑AAAACGCGACAATTCAACCCGcccccg                                                                                                                                                          >  1:42049/1‑248 (MQ=255)
                                                                                                     aTGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑GTTGTCGGTACAGGTAAGCCCCAGAT‑CGCCAAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCAGCGCCGTAc                                                                                                                                        <  1:174455/251‑1 (MQ=255)
                                                                                                     aTGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGCTACCGGGCTG‑GGTGACGGTACAGGGAAGAACCAGAAACGC‑AAGCCCCGCCATTCCCATTAAGGGGGG‑AGGGGGGGCACCAAGC‑GCCCCACC‑AAAGCGCGGCAATTCAACCAACCCACGGGTGagccacggcgta                                                                                                                                          >  1:339063/1‑239 (MQ=255)
                                                                                                        cgcCAGTGCGCGTACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCG‑C‑CTG‑CGCGCTTGGTCCATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑GTTGTCGGTACAGGTAAGCACCAGAT‑CGCCAAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCATCGCCGTAcgcg                                                                                                                                     <  1:460045/251‑1 (MQ=255)
                                                                                                         ccctgtccgctgctttcttTCGTATTGCGGTGGCCTTCC‑CCCCCCGACCCA‑T‑TTTT‑CTCTGTCGCGCTTTTTACATCCATGCCCTGCCCGACCTTCTTGCAAAACCGGCGGTTACGCGACTG‑GTTGTCGGCCCAGGAAAGCCGCAGAC‑CGCCAAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCAGCGCCGTACgcgc                                                                                                                                    <  2:414260/237‑1 (MQ=255)
                                                                                                                cgcgGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑GTTTGCGGTACAGGGAAGCACCAGAT‑CCCCAAGCCCCGCCCTGCCCCTAAAGGTGGC‑AGGGTCGGCACCCCAC‑GCCCCCCC‑CAGACGCCACCTTTCAGCCCGCCCACGGGGGG‑GCAGCGCCGGACGCGCA‑TTCGcc                                                                                                                            >  1:443499/1‑250 (MQ=255)
                                                                                                                  cgtaCTTCTTTCGTATTGCGTTAGCCTCCCCCCCCCTGACCAA‑TCTTCT‑C‑CGG‑CGCGCTTTTTACATCCTTGCCCTGACCGAGCATCACGCCAAAACGGCGGTTACGCGACTG‑GTTTTCGGCACTGGTAAGCACCAAAC‑CGCCAAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCAGCGCCGTACGCGCA‑TTCGCAcc                                                                                                                          <  2:334262/248‑1 (MQ=255)
                                                                                                                     aCTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAACCGGCGGTTACACGACTG‑GGTGGTGGTACCGGGAAGCACCAGAT‑CGCCAAGCCCCCCCATACCCCTCAAGGGGGG‑AAGGGCGGCACCCCGC‑GCCGCCCC‑CAGCCGCGACACTTAAGCCAGCCCCCCGGCGA‑TCCGCGCCCTCCCCGCA‑TTCGCAccca                                                                                                                        >  2:379399/1‑248 (MQ=255)
                                                                                                                       ttcttTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTAACGCGCCTG‑GGTGTCGGGAAAAGGACAAACCAGAA‑AGCCAACACCCGCCCTGCCCATAAAGGTGGC‑AGGGGCGGCACCCAGCCGCC‑CACC‑CAGACGCGCCATTTCCACCAGCCCCCGGGGGAGTCA‑CGCCACACCCGCC‑TTCGCACCAAAAc                                                                                                                     >  1:103238/1‑251 (MQ=255)
                                                                                                                         cttTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTT‑GTTGTCGGGACAGGGAAAAACCAAAT‑CGCCAAACCCCGCCCTGCCCCTAAAAGGGGC‑AGGGGCGGCACCCCGA‑GCCCCCCC‑CAGACGCGACATTTCAGCCAGCCCACGGGGGA‑GCAGCGCCCTACGCGCAATTC‑CACCaaaaacc                                                                                                                   >  1:38359/1‑248 (MQ=255)
                                                                                                                                  tGCGGTAGCCTTCCACCCCCTGACCAA‑TCTCCC‑C‑CTG‑CGCGCTTTGTACATCCTGGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGCCTG‑GTTGTCGGTACAGGTAAGCACCAGAT‑CGCCAAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCAGCGCCGTACGCGCA‑TTCGCACCAAAACCGATACCGTc                                                                                                           <  1:279947/250‑1 (MQ=255)
                                                                                                                                      gTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGGCTG‑GTTGTCGGTACAGGTAAGCCCCAGAT‑CGCCAAGCCCCGCCCTGCCCATAAAGGTGGCCAGGG‑CGGCACCCCGC‑GCCCCCCC‑CAGACGCGCCCTTTCAACCAGCCCACCGGGGA‑TCAGCGCCCTACGCGCC‑TTCGCCCCAAAACCGATACCGTCCGAcc                                                                                                      >  1:137416/1‑250 (MQ=255)
                                                                                                                                        aGCCTTCCACCACCTGACCAA‑GCTTCT‑C‑CTG‑CGCGCTTTGTACATCCAGGCCCGGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑GTTGTCGGTACAGGTAAGCACCAGAC‑CGCCAAGCCCCGCCATGCCCAGAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCAGCGCCGTACGCGCA‑TTCGCACCAAAACCGATACCGTCGGACAt                                                                                                     <  2:214401/250‑1 (MQ=255)
                                                                                                                                              ccacctCCTGACCAT‑GCTTCT‑C‑CTG‑TGCGCTTTGTACACCCATCCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGCCTG‑GTTGTCGTTACAGGGAAGCACCAGAT‑CGCCAACCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCATCGCCGTACGCGCA‑TTCGCACCAAAACCGATACCGTCGGACATCCCCGCa                                                                                              <  1:238890/251‑1 (MQ=255)
                                                                                                                                                 cccccgcaCCAA‑GCTTCC‑C‑CTG‑CGCGCTTTGTACACCCATGCCCGAACCGGCCATCATGCCAAACCGGGGGTTACGCGACTG‑GTTGTCGGAACGGGAAAGCCCCAGAG‑CGCCAAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCAGCGCCGTACGCGCA‑TTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCa                                                                                           <  2:42049/244‑1 (MQ=255)
                                                                                                                                                                  tttccccttcgcgcTTTTTACACCCATGCCCTGCCCGAGCACCATCCCAAACCGGCGGTTACGCGCCTG‑GTTGCCGTGACAGGAAAGCCCCACAT‑CGCCAAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCAGCGCCGTACGCGCA‑TTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACg                                                                               <  2:399442/241‑1 (MQ=255)
                                                                                                                                                                   t‑c‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTAAGCGACTG‑GGTGTCGGTAAAGGGAAGCACCAGGA‑CGCCAAGGCCCGCCATGCCCATTAAGGTGGC‑CGGGGCGGCACCCAGC‑GGCGCACC‑AAGACGCGACATTTCAGACAGCCCCCGGGAGA‑TAAGCGGCGTACGCGCA‑TTCGCACCAAAACCGAAAACGTCGGACATCCCCGCCACAATGGCaaaaaagctttt                                                                          >  1:450524/1‑241 (MQ=255)
                                                                                                                                                                    cctccttcccgcTTTGTACATCTATGCCCTACCCGACCATCATGCCAAACCGGCGGTTACGCAACTG‑GTGGTCGGTACAGGTAAGCACCAGAT‑CGCCAAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCAGCGCCGTACGCGCA‑TTCGCACCAAAACCGATACCGTCGGACATCCCCGCCCCAATGGCAATAACGt                                                                              <  1:497285/240‑1 (MQ=255)
                                                                                                                                                                     c‑cTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑GTTGGCGGTACAGGTAAGCACCAGAT‑CGCCAAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCAGCGCCGTACGCGCA‑TTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGtttt                                                                           <  1:94754/249‑1 (MQ=255)
                                                                                                                                                                     c‑cTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTAAGCGGCTG‑GTTGTCGGTACAGGGAAGCACCAGAT‑CGCCAAGCCCCGCCATGCCCATAAAGGGGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAAACGCGACATTTCAGCCAGCCCACGGGTGA‑TCAGCGGCGTACGCGCA‑TTCGCACCAAAAACGCAACCGTCGGGCATCCCCGCACCAATGGCAATAACGtttttt                                                                         >  1:419366/1‑250 (MQ=255)
                                                                                                                                                                     c‑cTG‑CGCGCTTTGTACATCCATGCCCGGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑GTTGTCGGTACAGGTAAGCACCAGAT‑CGCCAAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCAGCGCCGTACGCGCA‑TTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGttttt                                                                          <  1:148315/250‑1 (MQ=255)
                                                                                                                                                                               cTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑GTTGTCGGTACCGGGAAGCACCACAT‑CGCCAAGCCCCGCCCTGCCCATAAAGGTGGC‑AGGGGCGGCCCCCAGC‑GCCGCCCC‑AAGACGCGACATTTCAGCCAACCCACGGGGGG‑TCAGCGCCGCACGCGCA‑TTAGCACCACAACCGATACCGCCGGACATCCCCCCCCCAATTGCCATAACGTTTTTCAACGcgcc                                                                 >  1:474380/1‑251 (MQ=255)
                                                                                                                                                                                     aCATCCATGCCCTGACCGAGCAACATGCCAAAACGGCGGTTACGCGACTG‑GTTGTCGGTACAGGTAAGCACCAGAT‑CGCCAAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCAGCGCCGTACGCGCA‑TTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAg                                                           <  1:384024/251‑1 (MQ=255)
                                                                                                                                                                                       aTCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑GTTGTCGGTACAGGTAAGCACCAGAT‑CGCCAAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCAGCGCCGTACGCGCA‑TTCGCACCa                                                                                                                         <  1:28140/187‑1 (MQ=255)
                                                                                                                                                                                                   aCCGAGCATCATGCCAAAACGGCGGTTACGCGACGG‑GTTGCCGGTACAGGTAAGCACCAGAT‑CGCCAAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCAGCGCCGTACGCGCA‑TTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGa                                             <  1:207317/251‑1 (MQ=255)
                                                                                                                                                                                                      gAGCATCATGCCAAAACGGCGGTTACGCGACGG‑GTTGTCGGTACAGTAAAGCACCAGAT‑CGCCAAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCAGCGCCGTACGCGCA‑TTCGCACCAAAACCGATACCGTCGGACATcccc                                                                                                 <  1:317509/196‑1 (MQ=255)
                                                                                                                                                                                                       aGCATCATGCCAAAACGGCGGTTACGCGACTG‑GTTGTCGGTACAGGGAAACCCCAAAA‑CGCCCAACCCCGCCATGCCCCTAAAGGAGGG‑AGGGGCGGCACCCCGC‑GCCGCACC‑AAGACGCGACATTTTAGCCAGCCCACCGGGGA‑TCAGCGCCCTACGCGCC‑TTCCCACCCAAACCCAGACCGGCGGACATCCCCCCACCAACTGCAATAACGATTTTCACCGcgcccgcacgctgccaccccacgcaac                                          >  2:69123/1‑227 (MQ=255)
                                                                                                                                                                                                                   aaaCGGCGGTTACGCGTCTG‑GTTGTCGGTACAGGTAAGCACCAGAG‑CGCCAAGCCCCGCCCTGCCCATAAAGGTGGC‑AGGGGCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCCGCCAGCCCACGGGTGA‑TCAGCGCCGTACGCCCA‑TTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCGATAACGTTTTTCCCCGCCCCGCCAAACTGCACGCCAATGAAATCCGGAATGCTGt                              >  1:179854/1‑250 (MQ=255)
                                                                                                                                                                                                                    aaCGGCGGTTCCGCGACTG‑GTTGCCGGTACAGGTAAGCACCAGAT‑CGCCAAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCAGCGCCGTACGCGCA‑TTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTaa                            <  1:396931/251‑1 (MQ=255)
                                                                                                                                                                                                                      cggcggTTCCGCGTCGG‑GTTGTCGGTACAGGTAGGCACCAGAC‑CGCCAGGCCCCGCCATGCCCATAAAGGTGGC‑AGGGGCGGCCCCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCAGCGCCGTACGCGCA‑TTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAAc                          <  2:204455/251‑1 (MQ=255)
                                                                                                                                                                                                                       ggcggTTACGCGACTG‑GTTGGCGTAACAGTAAACCACAAGAC‑CGCCAAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCAGCGCCGTACGCGCA‑TTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAAcg                         <  2:233258/251‑1 (MQ=255)
                                                                                                                                                                                                                             tACGCGACCG‑GTTGTCGGTACAGGTAAGCACCAGAT‑CGCCAAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCAGCGCCGTACGCGCA‑TTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAACCGCGGaa                    >  2:258561/1‑250 (MQ=255)
                                                                                                                                                                                                                              aCGCGACTG‑GTTGTCGGTACAGGTAAGCACCAGAT‑CGCCAAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCAGCGCCGTACGCGCA‑TTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGaaa                   <  1:69123/250‑1 (MQ=255)
                                                                                                                                                                                                                                   aCTG‑GTTGTCGGTACAGGTAAGCACCAGAT‑CGCCAAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCAGCGCCGTACGCGCA‑TTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTg             >  1:296954/1‑251 (MQ=255)
                                                                                                                                                                                                                                    cTG‑GTTGTCGGTACAGGTAAGCACCAGAT‑CGCCAAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCAGCGCCGTACGCGCA‑TTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAAAGCGGAAACTTTTGc            >  2:407851/1‑251 (MQ=255)
                                                                                                                                                                                                                                               ggataGGTAAGCACCATAG‑CGCAAACCCCAGCCATGCCTATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGAAGCGACATTTCAGCCAGCCCACGGGTGA‑GCAGCGCCGTACGCGCA‑TTCGCACCAAATCCGATACCGTCGGACATCCCCGCACTAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgcag  <  2:206406/247‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                         
CGTGCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAA‑TCTTCT‑C‑CTG‑CGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑GTTGTCGGTACAGGTAAGCACCAGAT‑CGCCAAGCCCCGCCATGCCCATAAAGGTGGC‑AGGGTCGGCACCCAGC‑GCCGCACC‑AAGACGCGACATTTCAGCCAGCCCACGGGTGA‑TCAGCGCCGTACGCGCA‑TTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAG  >  NC_000913/3782667‑3783151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑