Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,731,7130GA30.0% 44.6 / 10.4 30E195K (GAA→AAA) xylFxylose ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (14/7);  new base A (0/9);  total (14/16)
Fisher's exact test for biased strand distribution p-value = 9.40e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTACGACCGTATGATTAACGATGCGGATATCGATTTTTATATTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACC‑TTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTT‑GATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGT  >  NC_000913/3731463‑3731935
                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                              
ttACGACCGTATGATTAACGATGCGGATATCGATTTTTATATTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACC‑TTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTT‑GATGGCTGGTTACCgg                                                                                                                                                                                                                                >  1:426594/1‑251 (MQ=255)
                aaCGATGCGGATATCGATTTTTATATTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACC‑TTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTT‑GATGGCTGGTTACCGGAAAACGCATTGaaaa                                                                                                                                                                                                                 <  1:41922/250‑1 (MQ=255)
                 aCGATGCGGATATCGATTTTTATATTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACC‑TTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTT‑GATGGCTGGTTACCGGAAAACGCATTGAAAAtt                                                                                                                                                                                                               <  1:68558/251‑1 (MQ=255)
                                    ttATATTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACC‑TTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTT‑GATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAAcc                                                                                                                                                                                             <  2:441996/250‑1 (MQ=255)
                                      atatTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACC‑TTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTT‑GATGGCTGGTTACCGGAAAACGAATTGAAAATTATGGAAAACGCGCTAACCGcc                                                                                                                                                                                          >  2:194381/1‑251 (MQ=255)
                                                          aaaGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACC‑TTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTT‑GATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGc                                                                                                                                                                      >  1:402677/1‑251 (MQ=255)
                                                                   gAACTGCAGGCAACAGCCCTGGACGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGCAGATAACAACGCCAAGCTCTTCCGCGCCGGACAAATGAAAGTGTTAAAACC‑TTACGTTGACTCCGGCAAAATTAAAGTCGATGGTGACCAATGGGTT‑GATGGCTGGTTACCGGAAAACGCATTGAACATTCTGGAAAACGCGCTAACCGCCAATAATAACa                                                                                                                                                                                >  1:365342/1‑232 (MQ=255)
                                                                            gCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACC‑TTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTT‑GATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGaaaa                                                                                                                                                                                                       >  2:520809/1‑200 (MQ=255)
                                                                            gCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACC‑TTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTT‑GATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGaaaa                                                                                                                                                                                                       <  1:520809/200‑1 (MQ=255)
                                                                                     cTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACC‑TTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTT‑GATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGc                                                                                                                                           >  2:128602/1‑251 (MQ=255)
                                                                                       ggTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACC‑TTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTT‑GATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGc                                                                                                                                           >  2:105091/1‑249 (MQ=255)
                                                                                          ggATATTGTCCCGCAAGATAATTACTTCCGGGTGGGCGGCTCGCCGTTAAATAACAACGCAAAGCTGTTCCGCGCCGGACAAATGAAAGTGTAAAAACC‑TTACGTTGATTCGGAAAAAATTAAAGTGGTTGGTGACCAATGGGTT‑GATGGCTGGTTACCGGAAAACGAATTGAAAATTAGGGAAAACGCGCAAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTg                                                                                                                                      <  2:426594/250‑1 (MQ=255)
                                                                                                      gCAAGGGAATTACTTCCTGATGGGCGGCTCGCCGGTCGATCACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACC‑TTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTT‑GATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAgg                                                                                                                          >  2:93072/1‑251 (MQ=255)
                                                                                                             aaTTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACC‑TTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTT‑GATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTa                                                                                                                     >  1:166638/1‑249 (MQ=255)
                                                                                                                     ccTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACC‑TTACGTTGATTCCGGAAACATTAAAGTCGTTGGTGACCAATGGGTT‑GATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCa                                                                                                             >  1:206745/1‑249 (MQ=255)
                                                                                                                        ggtGGGCGGCTCGCCGGTAGAAAACAACCCAAAGCTGTCCCGCGCCGGACAAATGAAAGTGTTAAACCC‑TTACGTTGATTCCGGAAAAATAAAAGTCGTTGGTGACAAATGGGTT‑GATGGCTGGTTACCGAAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAgg                                                                                                          <  2:166638/247‑1 (MQ=255)
                                                                                                                           gggCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACC‑TTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTT‑GATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTAt                                                                                                     >  2:445000/1‑251 (MQ=255)
                                                                                                                                        ataaaaaacaccGCCAAGTTTTTCCGTGCCGAAAAAATGAAGGTGTTAAAACC‑TTACGTTGTTTCAGAAAAAATTAAAGTGTTTGGTGACAAATGGTTT‑GAGGGTTGGTTACAGAAAAACGAATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGc                                                                                        <  2:402677/245‑1 (MQ=255)
                                                                                                                                         aaaaTAACAACGCAAAGCTTGCCCGCGCCGGACAAATAAAAGTTTAAAAACC‑TTACGTTTATTCCGAAAAAATTAAATTCGTTGGTGCCCAGGGGGTT‑GATGGCTGGTTCCCGAAAAACGCATTAAAAATTATGAAAAACGCGCAAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCa                                                                                       <  2:158908/248‑1 (MQ=255)
                                                                                                                                           gATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACC‑TTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTT‑GATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAAt                                                                                     >  2:451253/1‑251 (MQ=255)
                                                                                                                                                           tGTTCCGCGCCGGACAAATGAAAGTGTTAAAACC‑TTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTT‑GATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCAc                                                                                                                                              >  1:318432/1‑178 (MQ=255)
                                                                                                                                                              cccctgcgggACAAATGAAAGT‑TTAAAACCCTTACTTTGTTTCCGAAAAAATTAAAGTCGTTGGTGACCA‑TGGGTTTAATGGCTGTTTACCGGAAACCCCTTTAAAAATTATGGAAAACGCCCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGAt                                                                  <  1:93072/243‑1 (MQ=255)
                                                                                                                                                                           aaggaaatttttAAACCC‑TTACGTTGATTCCGAAAAAATTAAAGTCGTTGGTGACCAATGGGTT‑AATGGCTGGTTACCGAAAAACGCATTGAAATTTATGGAAACCGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTa                                                     <  1:447118/241‑1 (MQ=255)
                                                                                                                                                                           aaggAAAGTGTTAAAACC‑TTACGTTGATTCCGGAAAAATAAAAGGCGTTGGTGACCAAGGGGTT‑GAGGGCTGGTTCCCGAAAAACGCATTAAAAATTAGGAAAAACGCGCTACCCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTa                                                     <  1:445000/248‑1 (MQ=255)
                                                                                                                                                                                 aGTGTTAAAACC‑TTACGTTGATTACGGAAAAATTAACGTCGTTGGTGACCAATGGGAT‑GATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCACGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGt                                                >  2:392986/1‑250 (MQ=255)
                                                                                                                                                                                     ttaaaacctttagtttggtgccgaaaaaaTAAAAGTCTTTGGGGCCAAAGGGGTT‑GGTGGCGGGTAACCGGAAAACTAATTGAAAATTATGGAAAGCCGGCTACCCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGAGCGCGCAGGTATTAAACGTAtt                                             <  1:392986/226‑1 (MQ=255)
                                                                                                                                                                                        aaaCT‑TTACGTTTATTACGAAAAAATAAAAGCCGTTGGTGACAAATGGGTT‑TATGGCTGTGTACGGAAAAACGAATTTAAAATTATGAAAAACGACCAAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGGATTAAACGTATtgctgc                                        <  2:37378/251‑1 (MQ=255)
                                                                                                                                                                                                            aaaaaattaaatgcgtgggggaaaaaTGGGTT‑GATGGCTGTTTACCGAAAAACGAATTGAAAATAATGAAAACCCCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAATCGATGCCACCGCATGTGGGGCAAGTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACg                     <  1:105847/227‑1 (MQ=255)
                                                                                                                                                                                                                    aaaGTCGTGGGTGACAAATGGGTG‑GATGGCGGGTTACCGAAAAACGCATTGAAATTTATGAAAAACGCGTTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATaa             <  1:171769/251‑1 (MQ=255)
                                                                                                                                                                                                                          gTTGGTGACCAATGGGTT‑GATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTAt       >  2:305879/1‑251 (MQ=255)
                                                                                                                                                                                                                               ggACCAATGGTTT‑GAGGGCTGGTTCCCGAAAAACGCATAAAAAATTATGAAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACgt  <  1:424068/250‑1 (MQ=255)
                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                              
TTACGACCGTATGATTAACGATGCGGATATCGATTTTTATATTTCTTTCGATAACGAAAAAGTCGGTGAACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGCTCGCCGGTAGATAACAACGCCAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACC‑TTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCAATGGGTT‑GATGGCTGGTTACCGGAAAACGCATTGAAAATTATGGAAAACGCGCTAACCGCCAATAATAACAAAATTGATGCTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGT  >  NC_000913/3731463‑3731935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑