Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,147,745	0	G	T	25.9%	115.6 / 10.3	59	L24F (TTG→TTT)	yegD	HSP70 family protein YegD
Reads supporting (aligned to +/- strand):  ref base G (14/29);  new base T (15/0);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 5.21e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAGGCGCTGTTACGTCGGGCGATTCGTTATAACCGCGAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGTGGTTTGTGAAAT  >  NC_000913/2147518‑2147995                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                 ggcTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATTCGTG‑TC‑GG‑TGAAACGC‑C‑TTTTCT‑TAAAA‑T‑AGAAAAAGACAacac                                                                                                                                                                                                                                        >  2:131804/1‑251 (MQ=255) ggcTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCAc                                                                                                                                                                                                                                        <  2:168350/251‑1 (MQ=255)  gcTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACg                                                                                                                                                                                                                                       <  1:21585/251‑1 (MQ=255)            cAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑GC‑GG‑GGAAACGC‑A‑TTTTCT‑TAAAA‑T‑GGGAAACAACAGCCCGCTGCTGCC‑tt                                                                                                                                                                                                                            >  1:547423/1‑251 (MQ=255)              gggTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGACGGCAACATTTCGCTAAAGGCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCAGGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCa                                                                                                                                                                                                                          <  2:500257/251‑1 (MQ=255)                gTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAAt                                                                                                                                                                                                                        <  1:131804/251‑1 (MQ=255)                   taCATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATTCGTG‑GC‑GG‑GAAAACGC‑C‑CTTTCT‑AAAAA‑T‑TGGAAACGACaaaccccttccgccctccctta                                                                                                                                                                                                                       >  2:439780/1‑229 (MQ=255)                   taCATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAAt                                                                                                                                                                                                                        <  1:439780/248‑1 (MQ=255)                   taCATCGCATCTCCTTATTCATCAGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATCTTCCGGATGTCGCGACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGGCATTCGTG‑GC‑GG‑TAAACAGC‑C‑TTTTCT‑AAAAA‑T‑TTGAAACGGCAACACGCTGCGGccctccatact                                                                                                                                                                                                                      >  2:85722/1‑242 (MQ=255)                      aTCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑GC‑GG‑GAAAACCC‑A‑TTTTCTTAAAAA‑G‑GGAAAA‑GACAGCACGCCGCTGCC‑TTCAATGCtttt                                                                                                                                                                                                                  >  1:492857/1‑250 (MQ=255)                         gCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATTCGTG‑AC‑GG‑GAAAACGA‑C‑TTTTCT‑TAAAA‑A‑GGAAAACGACAGCACGCTGCTGCC‑TTcc                                                                                                                                                                                                                          >  1:140461/1‑239 (MQ=255)                         gCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCa                                                                                                                                                                                                                          <  2:140461/240‑1 (MQ=255)                            tctcCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑GC‑GG‑GAAAACGC‑C‑TTTTCT‑TAAAA‑T‑AGAAAACGACAGCCCGCTGCTGCC‑TTCAATGCTTTTCGCGcc                                                                                                                                                                                                            >  1:63998/1‑251 (MQ=255)                                   aTTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGGCATTCGTG‑AC‑GG‑GAAAACGC‑C‑TTTGCT‑AAAAAAT‑GGAAA‑CGACAGCACCCCGCTGCC‑TTCaaatcttttcgccgcaacccgg                                                                                                                                                                                                     >  1:306045/1‑231 (MQ=255)                                     tCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGTGTG‑AC‑GG‑GAAAACAC‑ACTTTCCT‑AAAAA‑‑‑AGAAAACAAGAACACGCCGCCGCC‑TTCAAtcggtttcgcgccccaacgtcg                                                                                                                                                                                                   >  1:547629/1‑230 (MQ=255)                                          cGCTTTCAGAATAACCGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCAATCGGG‑AA‑GG‑GAAAACGCCG‑TTTGTT‑AAAAA‑‑‑GGAAAAACACAGCACGCCGCTGCC‑CTCCATGATTTTCGCGcccaccggtggcgagg                                                                                                                                                                                              >  1:257688/1‑237 (MQ=255)                                                 aGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCCTTCGGG‑ACCGG‑AAAACC‑C‑C‑TTTTCT‑TAAAA‑T‑GGAAAAAAACAACACGCTGCCGCC‑CTTAATGCTTTTTGCGCCAACGCGTGGAGCggggagcg                                                                                                                                                                                        >  1:299343/1‑244 (MQ=255)                                                               tCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATTCGTG‑GG‑GGGTAAAC‑GC‑C‑TTTGTT‑TAAAA‑T‑GGGAAAAGACACCCCGCTGCGGCC‑CTCAATGCTTTGCGCGGCAAAGAGGGAAGCGGTAAGAG‑aaatggtcgtacgcc                                                                                                                                                                        >  2:328449/1‑238 (MQ=255)                                                                        gcgcTTTGCTTTCATATTCCGGTTGTCGCGACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCAGCatgatg                                                                                                                                                              <  1:48890/251‑1 (MQ=255)                                                                          gctttgctttCATATTCCGGTTGTCGCGCCGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTGGTG‑GG‑TAAAC‑GC‑C‑TTTGCT‑AAAAA‑TTGGAAAA‑GCAAGCACGCCGCCGCC‑TTCAATGCGTTTTGCGCCCACCCGTGAAACGGGCAGCG‑CATTGCTG‑TTCCCCCCACATGATGtt                                                                                                                                                            >  2:94593/1‑251 (MQ=255)                                                                                  ttCATATTCCGGTTGGCGCGACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCa                                                                                                                                                      <  2:492857/249‑1 (MQ=255)                                                                                            ggTTGTCGCGACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATTCGTG‑GC‑GG‑TAAAACCC‑A‑ATTTCT‑AAAAA‑A‑GGAAAACGACAACAAGCTGCTGCC‑CTCTATGCTTTGCGCGCCAACGCGGGAAGCGGTAAGCG‑GAAGGGTG‑GAACCCCATCATGATGTTCCGGGAgacgaaggtga                                                                                                                                           >  1:18760/1‑244 (MQ=255)                                                                                            ggTTGTCGCGACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑GC‑GG‑GAAACCGC‑A‑TTTTCT‑AAAAA‑A‑GGGAAACAACAGCCCCCTCCTGCC‑TTCAATGCTTTGTGCGCCCACCCCGGAAGCGGGAAACG‑GATGGCTGGTAC‑GCCATCatgatggttcggccaccgaccgtgg                                                                                                                                           >  2:202196/1‑231 (MQ=255)                                                                                             gTTGTCGCGACGGCAACATTTCGCTAAAGGCACTCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCGTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGaaa                                                                                                                                         <  2:355783/251‑1 (MQ=255)                                                                                                  cgcgACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCa                                                                                                                                     <  2:209309/250‑1 (MQ=255)                                                                                                  cgcgACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCa                                                                                                                                     <  2:101557/250‑1 (MQ=255)                                                                                                  cgcgACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAg                                                                                                                                    <  1:269036/251‑1 (MQ=255)                                                                                                    cgACGGCAACATTTCGCTAAAGTCCTGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGTTTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCTTCATTATGTTCCGGCAGACGACGATGAAACGCAGgc                                                                                                                                  <  2:457116/251‑1 (MQ=255)                                                                                                    cgACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGGG‑AA‑GG‑TGAACCGC‑C‑TTTTCT‑AAAAA‑T‑AGAAAAAGAAAGAACGCCGCTGCC‑TTTCATGCTTTGGCCGCCAAAGCGTGGAGCGGGAAGCG‑GATTGCTG‑TACCGCCATCATGCTGTTTTCGCAGACGACGGTGaaaaacaagg                                                                                                                                  >  1:377421/1‑244 (MQ=255)                                                                                                        ggCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑GAAACCGC‑A‑TTTTCT‑AAAAA‑T‑GGAAAACGACAACACGCCGCTGCC‑TTCAAAGCTTTTCCCGCCCAAGCGTGAAACGGGAAGGG‑GATGGCTG‑TACCCCCCTCATGGTGTTCCGGCCAAcgacgaaggaaagccggcgccg                                                                                                                              >  1:473022/1‑235 (MQ=255)                                                                                                         gCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATACGTG‑GC‑GG‑GAAAACGC‑A‑TTTTCT‑AAAAA‑T‑GGAAAACGAAAACACGCTCCTGCC‑CTCAATGCTTTTCGCGCCCACCCGTGAAAGGGGAAGGG‑AATGGCTG‑GGACGCCCTCATTATTTTCCGGGAGACCACGGTGAAAAGCAGGGGCTGt                                                                                                                             >  1:145538/1‑251 (MQ=255)                                                                                                          cAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAGGCGCTGtt                                                                                                                            <  1:156795/251‑1 (MQ=255)                                                                                                          cAACATTGCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGGTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑GTCAATGCGTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TAGCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAGGCGCTGtt                                                                                                                            <  1:543198/251‑1 (MQ=255)                                                                                                                  cGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAGGCGCTGTTACGTCgg                                                                                                                     <  1:451248/250‑1 (MQ=255)                                                                                                                   gCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAGGCGCTGTTACGTCGGGc                                                                                                                   <  1:322774/251‑1 (MQ=255)                                                                                                                       acgtctcGCCCCTTATTCACCGGCATGGGGATTATTTCGTGTTTATTGGTGTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AAGGGCGG‑TACCGGCATCATGATGTGCCGGCAGACGACGATGAAACGCAGGCGCTGTGACGTCGGGCGAtt                                                                                                               <  2:271965/245‑1 (MQ=255)                                                                                                                                        cACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAGGCGCTGTTACGTCGGGCGATTCGTTATAACCGCgaag                                                                                               <  2:407363/250‑1 (MQ=255)                                                                                                                                                       tattTCGTGTTTATTGGGTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAGGCGCTGTTACGTCGGGCGATTCGTTATAACCGCGAAGAAGATATCGATGTTa                                                                                <  2:431242/250‑1 (MQ=255)                                                                                                                                                        attTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAGGCGCTGTTACGTCGGGCGATTCGTTATAACCGCGAAGAAGATATCGATGTTAc                                                                               <  1:87729/250‑1 (MQ=255)                                                                                                                                                        attTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAGGCGCTGTTACGTCGGGCGATTCGTTATAACCGCGAAGAAGATATCGATGTTAc                                                                               <  2:551028/250‑1 (MQ=255)                                                                                                                                                             gtgtTTATTGGTTTTGATTCCGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAACCGGCAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAGGCGCTGTTACGTCGGGCGATTCGTTATAACCGCGAAGAAGATATCGATGTTACGGCGaa                                                                         <  2:303408/251‑1 (MQ=255)                                                                                                                                                                  tATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATTGGTG‑AC‑GG‑TAAAACGC‑A‑TTTGGT‑AAAAA‑T‑TGAAAACGACATCACGGTGCTGCC‑TTCAATGCTTTGAGCGCCAACCCGTGAAGCGGTAAGGG‑AATGGCTG‑TTCCGCCCTCATGATTTTCTGGCAGACGACGAAGGAAAGCAGGGGCCGTTTCGGCGGGGGCATCGTTATAAAACCGGAGAAGAGATCGAGGTTACggggcacaagg                                                                    >  2:385057/1‑242 (MQ=255)                                                                                                                                                                   aTTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGGAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAGGCGCTGTTACGTCGGGCGATTCTTTATAACCGCGAAGAAGATATCGATTTTACGGCGAAAAGCg                                                                    <  2:550742/250‑1 (MQ=255)                                                                                                                                                                      ggTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAGGCGCTGTTACGTCGGGCGATTCGTTATAACCGCGAAGAAGATATCGATGTTACGGCGAAAAGCGTGc                                                                 <  1:101182/250‑1 (MQ=255)                                                                                                                                                                        ttttGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTT‑AT‑GG‑TTAAACGC‑C‑TTTTCT‑AAAAA‑TTGGAAAA‑GTCAGCACGCTCCTGCCGTTCAA‑GGTTTTTGCCCCCACACGGGGAGCGGTAAGCGGAATGGTTG‑‑ACCGCCATCATGATGTTTCGGGAGCCGACGGTGAAACGCAGGCGATGGTTCGTCGGGGGATTCGCTAAAACCACGGAGAAGAGATCTATggtataggcaaaatggggcagg                                                              >  2:180111/1‑230 (MQ=255)                                                                                                                                                                            gATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTTCT‑TAAAA‑T‑GGGAAACGACAGCACGCTGCCGCC‑TTCAATGCTTTTCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCAACATGATGTTCCGGGAGACGACGATGAAAAGCAGGCGCTGTTACGTAGGGCGCTTCGTTTTAACCCCGAAAAAGATATCGATGTTTCGGCGAAAAGCggggcggtcgg                                                          >  1:6997/1‑241 (MQ=255)                                                                                                                                                                                     aCAGCAAACTGTTCAGTGGCGGTCATGCGGG‑AC‑GG‑GAAAACGC‑C‑TTTGCT‑AAAAA‑A‑AGAAAAAGAAAGCACGCTGCTGCC‑TTCACTTCTTTTCGCGCCCACGCGCGAAGCGGGAAGCG‑AAAGGGTG‑TACCGCCCTCATGATGTTCCGGCAGCCGACGATGAAAAGCAGGCGCTGTTACGTCGGGCGGTTTGTTTTAAACGCGCAGAAGAGATAGATGTTAAGGCGgaaaacgggcgggtcgggcttttctc                                                 >  1:545161/1‑229 (MQ=255)                                                                                                                                                                                               gTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGTTG‑TACCGGCATCATGATGTTCCGGAAGACGACGATGAAACGCAGGCGCTGTTATGTCGGGCGATTCGTTATAACCGCGAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAg                                       <  1:316208/251‑1 (MQ=255)                                                                                                                                                                                                         ggTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAGGCGCTGTTACGTCGGGCGATTCGTTATAACCGCGAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACCTtgatg                             >  2:198165/1‑251 (MQ=255)                                                                                                                                                                                                         ggTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAGGCGCTGTTACGTCGGGCGATTCGTTATAACCGCGAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATtgatg                             <  2:172165/251‑1 (MQ=255)                                                                                                                                                                                                            cATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGAAAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAGGCGCTGTTACGTCGGGCGATTCGTTATAACCGCGAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATc                          >  1:216282/1‑251 (MQ=255)                                                                                                                                                                                                             aTGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAGGCGCTGTTACGTCGGGCGATTCGTTATAACCGCGAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATcc                         >  2:168841/1‑251 (MQ=255)                                                                                                                                                                                                                cGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAGGCGCTGTTACGTCGGGCGATTCGTTATAACCGCGAAGAAGATATCGATGTTACGGCGAAAAGCGGGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATcc                         >  1:488436/1‑248 (MQ=255)                                                                                                                                                                                                                     aC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTGCCGGCAGACGACGATGAAACGCAGGCGCTGTTACGTCGGGCGATTCGTTATAACCGCGAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTGTCCTCACTGGCACAGTACATTGATGATCCAgaagaa                  <  2:161524/251‑1 (MQ=255)                                                                                                                                                                                                                        gg‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAGGCGCTGTTACGTCGGGCGATTCGTTATAACCGCGAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAgt                >  1:300318/1‑251 (MQ=255)                                                                                                                                                                                                                            aaaCCGC‑A‑TTTGCT‑AAACA‑T‑GGAAAACGACAGCCTGCTGCTTCA‑TTCAAAGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAGGCGCTGTTACGTCGGGCGATTCGTTATAACCGCGAAGAAGATATAGATGTTACGGCGAAAAGCGCGCTGTTCGGTCTTTCATAACTGGACCAGACCATTGTGGATCCAGAGGAAGtgtg             >  2:66605/1‑251 (MQ=255)                                                                                                                                                                                                                            aaaCCGC‑A‑TTTGCG‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAGGCGCTGTTACGTCGGGCGATTCGTTATAACCGCGAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAgtgt              <  1:262543/250‑1 (MQ=255)                                                                                                                                                                                                                               ccGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAGGCGCTGTTACGTCGGGCGATTCGTTATAACCGCGAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGTGGtt          >  1:236425/1‑251 (MQ=255)                                                                                                                                                                                                                               ccGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAGGCGCTGTTAAGTCGGGCGATTCGTTATAACCGCGAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGTGGtt          >  2:469906/1‑251 (MQ=255)                                                                                                                                                                                                                                         gCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAGGCGCTGTTACGTCGGGCGCTTCGTTATAACCGCGAAGAGGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTCCATTGATGATCCAGAAGAAGTGAGGTTTGTGAAAt  >  1:453898/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                 GGCTGCCAGTTCAGGGTATACATCGCATCTCCTTATTCATCCGCTTTCAGCATAAACGTTATTCAGACGCTGCGCTTTGCTTTCATATTCCGGTTGTCGCGACGGCAACATTTCGCTAAAGTCACGCCCCTTCTTCACCGGCATGGGGATTATTTCGTGTTTATTGGTTTTGATTACGGTACAGCAAACTGTTCAGTGGCGGTCATGCGTG‑AC‑GG‑TAAACCGC‑A‑TTTGCT‑AAAAA‑T‑GGAAAACGACAGCACGCTGCTGCC‑TTCAATGCTTTGCGCGCCAACGCGTGAAGCGGTAAGCG‑AATGGCTG‑TACCGCCATCATGATGTTCCGGCAGACGACGATGAAACGCAGGCGCTGTTACGTCGGGCGATTCGTTATAACCGCGAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGTGGTTTGTGAAAT  >  NC_000913/2147518‑2147995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑