Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,029,2740CT34.5% 35.7 / 10.7 30K31K (AAG→AAA) yedIDUF808 domain‑containing inner membrane protein YedI
Reads supporting (aligned to +/- strand):  ref base C (3/16);  new base T (10/0);  total (14/16)
Fisher's exact test for biased strand distribution p-value = 1.43e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCGCCTCCAGCATATGCAGCACTTTCTCTACTCCTTCAAAGCAGAGAAACGCGCCACCAATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTC‑GCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTG‑TGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTT‑GAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGC‑TTCACAGGTCAA‑CATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACG  >  NC_000913/2029024‑2029498
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                     
gcgcCTCCAGCATATGCAGCACTTTCTCTACTCCTTCAAAGCAGAGAAACGCGCCACCAATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTttc                                                                                                                                                                                                                                       <  1:187833/251‑1 (MQ=255)
  gcCTCCAGCATATGCAGCACTTTCTCGACTCCTTCAAAGCAGAGAAACGCGCCACCAATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACGTTATTAATCAGCGAGCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTttctt                                                                                                                                                                                                                                     <  1:225930/251‑1 (MQ=255)
       cAGCATATGCAGCACTTTCTCTACTCCTTCAAAGCAGAGAAACGCGCCACCAATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTc‑gccg                                                                                                                                                                                                                               <  1:58289/251‑1 (MQ=255)
               gcagcaCTTTCTCTACTCCTTCAAAGCAGAGAAACGCGCCACCAATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTC‑GCCGCCAGttt                                                                                                                                                                                                                        <  2:156598/250‑1 (MQ=255)
                      tttCTCTACTCCTTCAAAGCAGAGAAACGCGCCACCAATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTTTTC‑GCCCCCAGTTTgtccccc                                                                                                                                                                                                                 >  2:439955/1‑244 (MQ=255)
                            tACTCCTTCAAAGCAGAGAAACGCGCCACCAATCATCAACAGAGGCGTAATCGCCCAAGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTGTTCTTT‑GCCCCCAGttttgccactccggagg                                                                                                                                                                                                          >  2:324492/1‑237 (MQ=255)
                                            agaAACGCGCCACCAATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTC‑GCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAgtg‑t                                                                                                                                                                                         <  1:269868/251‑1 (MQ=255)
                                              aaaCGCGCCACCAATCATCCACAGAGGCGCAATCGCCCACGGGATAAACGCACTGATGATAAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGACACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTTTTT‑GGCCCCCGTTTGTCCATTAACGAGATATCGTcccatacggcgg                                                                                                                                                                                        >  2:547716/1‑241 (MQ=255)
                                              aaaCGCGCCACCAATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGCTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTTTTT‑GCCGCCCGTTTGCCCATCCCGGGGATATCGTCCAGTAgtgggg                                                                                                                                                                                        >  1:63113/1‑248 (MQ=255)
                                                   cgcCACCAATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTC‑GCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTG‑TGGCGata                                                                                                                                                                                  <  1:388100/251‑1 (MQ=255)
                                                       accaATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTC‑GCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTG‑TGGCGATatcat                                                                                                                                                                              <  1:47106/251‑1 (MQ=255)
                                                       accaATCAGCAACAGAGGCTTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTGCTTC‑GCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTG‑TGGCGATatcat                                                                                                                                                                              <  2:35478/251‑1 (MQ=255)
                                                                      agGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTC‑GCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTG‑TGGCGATATCATCGAGCAACGTCAGTa                                                                                                                                                               <  1:141401/251‑1 (MQ=255)
                                                                        gCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTC‑GCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTG‑TGGCGATATCATCGAGCAACGTCAGTaaa                                                                                                                                                             <  1:211168/251‑1 (MQ=255)
                                                                                     cGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTTTTC‑GCCGCCAGTTTTCCCATCACGGGGATATCGTACTGTAGTG‑GGGGGATATCATCCAGCAAAGTCAGTAAACTACTccccggc                                                                                                                                                 >  1:77080/1‑246 (MQ=255)
                                                                                                  cTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTTTTC‑TCCGCCCGTTTTCCCATCACGGGGATTTCGTCCCGGGGGG‑TGGGGGTATCATGGGGGCACGTCAGTAAACTACTCCCGGCCAAAAACAATccccc                                                                                                                                   >  2:26074/1‑249 (MQ=255)
                                                                                                    gatgatCAGCGCCAGCGGCACCAGAATCTCTTTATTAATCAGCGCTCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCCCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGCTATCCCCTAATACGCCGGCGGTTTTCTTC‑GCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTG‑TGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCctc                                                                                                                                   <  2:336898/249‑1 (MQ=255)
                                                                                                               ccAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTTTTC‑GCCGCCAGTTTGCCCATCACCGGGATATCGTCCCGCAGTG‑TGGCGATTACATCGAGCCACGGCAGTAAACTACTCCCGGCCCAAAAAATTTCCTCTCACTTTTTttgt                                                                                                                      >  1:461054/1‑251 (MQ=255)
                                                                                                                     gCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTTTTT‑GCCCCCCGTTTGCCCATCAACGAGATATCCTCCCCTACAG‑TGGCGATATCATCGAGCAACGTCAGTAAACTTATCCCGGCCACAAGCATTCCCTCCCCTTTTTATTTTT‑TAAgg                                                                                                               >  1:62295/1‑251 (MQ=255)
                                                                                                                            aagcACTTTATTAATCAGCGATCCTTGCGCCACGCCCCAGGCCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATCGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTC‑GCCGCCAGTTTGCCCATCACGGAGATAGCGTCCAGTAGTG‑TGGCGATATCATCAAGCAACGTCAGTAAGCTACTCCAGGCCAAAGGCATTCCCTCTCATTTTTATTGTT‑GAAGGGTGTAAg                                                                                                        <  2:315628/248‑1 (MQ=255)
                                                                                                                               cACTTTATTAATCAGCGATCCTTGCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACGGGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTC‑GCCGCCAGTTTGCCCATCACGGAGAGATCGTCCAGTAGTG‑GGGCGATATCATCGAGCAACGTCAGGAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTAGTGTT‑GAAGGGTGGAAGTa                                                                                                      <  2:518131/250‑1 (MQ=255)
                                                                                                                                       taATCAGCGATCCTTTCGCCACGCCCCAGTCCACGGGAAGTGCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTC‑GCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTG‑TGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTT‑GAAGGGTGTAAGTATGAAGCaa                                                                                              <  2:7939/250‑1 (MQ=255)
                                                                                                                                            aGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTTTTC‑GCCGCCCGTTTGCCCCTCACGGGGAACTCGTCCAGATGGG‑TGGGGATATCATCGGGCAACTTCAGTAAACTCATCACGCCCCAAAACAATCCCTCTCATTTTTTTTGGT‑GTAGGGGGGAAGTAATAAGAAAAAAcac                                                                                        >  2:445101/1‑249 (MQ=255)
                                                                                                                                                          cACGCCCCACACCCCGGGCTGTTGCCGGTCGGCCCGCCCGCCTGAACCTTGTTGCGCATTGCGCGATAAGTCATCCCCTAATACACCGGCGGTTTT‑TTCTGCCGCCCGGCTGCGCACCACCGAGATAACGCCCAGGAGAG‑GGGCGGTATCCTCGCGCACAGTAAGTAAACTACCCCCGGCCCCAAGAATTCCCTCTCCTTTTTTTTTTT‑TTAGGGGGTGAGTATGAACCACAAACCGCAAGTCTGATaatt                                                                          >  2:490856/1‑248 (MQ=255)
                                                                                                                                                           aCGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTC‑GCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTG‑TGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTT‑GAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTg                                                                         <  1:368702/251‑1 (MQ=255)
                                                                                                                                                                                        ggCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTC‑GCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTG‑TGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTT‑GAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGC‑TTCACAGGTCAA‑CATTTTTTTAACAAt                                           <  1:212383/250‑1 (MQ=255)
                                                                                                                                                                                                 gCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACCCCGGCGGCTTTCTTC‑GCCGCCAGTTTGACCATCCCGGAGATATCGGCCCGGAGTGCTGG‑GATATCATTGAGGAACCTCAGGAAAATACTCCCGGCCCAAAGCATTCCCTCTCATTTTTTTTGTTTGAAGGG‑GTTAGGATGAAGAAAAAACAGCCGCCCTTATACTTGG‑CTCACCGGTCAA‑CATTTTTTTTATAAACAAGTGCAAt                                 >  1:85700/1‑251 (MQ=255)
                                                                                                                                                                                                                cgcATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTTTTT‑GCCGCCAGTTTGCCCATCACGGAGAAATCGGCCAGTAGTG‑TGGCGAAATCATCGAGCAACGTCAGTAAAATAATCCCGGCCAAAAACATTCCCCCTCATTTTTATTGTT‑TGAGGGGGGAAGTATGAAGAAAAAAAGGCAGCTCTGATACCTGC‑TTCACAGGTAAAACTTTTTTTTAA‑AATAAAATGCAATTATATTTCTCGcc                    >  1:367219/1‑249 (MQ=255)
                                                                                                                                                                                                                     tGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTTTTC‑TCCGCCCGTTTGCCCCTCACGGAGGAATTGTCCAGGCGTG‑GGGGGATATCATCGTGCAACGGCAGTAAACTAACCCCGGCCCAAAGCAATTCCTCTCCTTTTTATTGTT‑GACGGGTGGAAGTATGAAGAAAAAACGGCAGATCTTATACTTTCGTTCCCGGGCCAA‑‑ATTTTTTTAACAATTAAGTGCAATTATATTTCTCGCcaaaaga              >  1:374875/1‑245 (MQ=255)
                                                                                                                                                                                                                         cGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTC‑GCCCCCCGTTTGCCCATCACGGAGATATCCTCCAGTAGAG‑TGGCGATATCATCGCGCAACGTCAGAAAACTACTCCCGGCCCAAAACATTCCCTCTCCTTTTTTTTGTT‑TAAGGGGGTAAGGATGAAGCAAAAACGGCAGCTCTTATACTTGC‑TTTACAGGGCAA‑CAATTTTTTTACAAACAAATGACATTTTATCTCTCGCCAGCAGAATGAt         >  1:480565/1‑251 (MQ=255)
                                                                                                                                                                                                                           aTAAGTCATCCCCTAATACACCGGCGGTTTTCTTC‑GCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTG‑TGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTT‑GAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGC‑TTCACAGGTCAA‑CATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGAttt       <  1:89749/251‑1 (MQ=255)
                                                                                                                                                                                                                                 cATCCCCTAATACACCGGCGGTTTTCTTC‑GCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTG‑TGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTT‑GAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGC‑TTCACAGGTCAA‑CATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACg  <  1:365792/250‑1 (MQ=255)
                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                     
GCGCCTCCAGCATATGCAGCACTTTCTCTACTCCTTCAAAGCAGAGAAACGCGCCACCAATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTC‑GCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTG‑TGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTT‑GAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGC‑TTCACAGGTCAA‑CATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACG  >  NC_000913/2029024‑2029498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑