Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913646,7770GT35.5% 31.0 / 13.7 31Q245K (CAA→AAA) citGtriphosphoribosyl‑dephospho‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base G (5/15);  new base T (11/0);  total (16/15)
Fisher's exact test for biased strand distribution p-value = 6.77e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CATCACCACCAGTGGGGCCAATAGTTTCCATATATTATCTTTTGCTAAAGACATACGGGTTCTCCGAAAATTAATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCC‑TTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTGAGGTAATGCGGCAAGGCGTGATTGATCACC‑AGTGGATAACCCGCTTCGGCTTCACCGCGTGCGCCGGTAAGGCCAAGCTGTTGGTACAACCGTTGACCTGCCGTCAG  >  NC_000913/646527‑647020
                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    
cATCACCACCAGTGGGGCCAATAGTTTCCATATATTATCTTTTGCTAAAGACATACGGGTTCTCCGAAAATTAATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTATGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTGCGATACACTCCCTGTCGAGCTGCCGGAGATAATCGAAATCGGCGGGGGTTCGAAGGCCCCC‑TTGTTGCAATAACGTTTg                                                                                                                                                                                                                                                      <  2:107267/251‑1 (MQ=255)
     ccaccaGTGGGGCCAATAGTTTCCATATATTATCTTTTGCTAAAGACATACGGGTTCTCCGAAAATTAATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTGCAATGCCCCC‑TTTTTGTACTAATGTTTTCGccc                                                                                                                                                                                                                                                 >  1:218389/1‑250 (MQ=255)
                          tCCATATATTATCTTTTGCTAAAGACATACGGGTTCTCCGAAAATTAATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCC‑TTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAgcg                                                                                                                                                                                                                                 <  1:285810/246‑1 (MQ=255)
                          tCCATATATTATCTTTTGCTAAAGACATACGGGTTCTCCGAAAATTAATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGCATGCCCCC‑CTTTTGTAATAATGTTTGTGCCCCGCGCCGTAGCCAgcgggaac                                                                                                                                                                                                                            >  2:285810/1‑246 (MQ=255)
                                 attatCTTTTGCTAAAGACATACGGGTTCTCCGAAAATTAATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCC‑TTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTc                                                                                                                                                                                                                     <  2:138407/251‑1 (MQ=255)
                                               aaGACATACGGGTTCTCCGAAAATTAATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAAAGCCCCCCTTTTTTAAATA‑TGTTTTTGCCCCCCGCTGTAGCCCGCGCCGGccccccccccccacgcccgatg                                                                                                                                                                                                       >  1:210872/1‑235 (MQ=255)
                                                        gggTTCTCCGAAAATTAATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGGATGCCCCC‑CTTTTGCAAAAATTTTTTCGCCCCCCCCTGTAGCCAGCGCCGGCACCCCCCCCCAccccccgctggcacggtgg                                                                                                                                                                                              >  1:462580/1‑234 (MQ=255)
                                                           ttCTCCGAAAATTAATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGAATACCCCC‑TTTTTGCAATAATGTTTTCGCCTCGCGCTGTAGCCAGAGCAGGGCCCCCCCGcccccccccgatcgaaagttggggt                                                                                                                                                                                           >  1:477973/1‑228 (MQ=255)
                                                                    aaTTAATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCC‑TTTTTGCAATAATGTTTGCGCCTCGCGCTGTa                                                                                                                                                                                                                                        <  1:189392/197‑1 (MQ=255)
                                                                                  aaTTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGCATGCCCCC‑TTTTTTCAATAATGTTTTCGCCCCCCGCTGTAGCCAGCGCCGGCCCCCCTCCCCCCCCCCCGGTGCACCGGTGGGATCGCCGTTGTTCGCCATCAGttgg                                                                                                                                                                    >  1:252366/1‑248 (MQ=255)
                                                                                                      aatTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGACCCC‑TTTTTTCACTAATGTTTTCGGCTCCCGCTGTAGCCCGCCCAAGCCCCCCCCGCCCCCCCGCGATCCCAAGGTGGGATCGCCGCTGGTCGCCCTCAGtgagagcgaggggatgagcaatgc                                                                                                                                                >  1:176486/1‑228 (MQ=255)
                                                                                                            aTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCC‑TTTTTTCAATAATGTTTTTGCCCCGCGCTGTAGCCCACGCAGGCCCCCCCCGCCCCCGCCCGAAGCAAAGGTGGGTTCGCCCTTGGTCGCCCACCGTAGGGGCAAGGAATCGAGCAATGCCCGTTc                                                                                                                                          >  1:35213/1‑251 (MQ=255)
                                                                                                                gtgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCC‑TTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAg                                                                                                                                        <  1:316388/249‑1 (MQ=255)
                                                                                                                gtgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCC‑TTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGa                                                                                                                                      <  1:208148/251‑1 (MQ=255)
                                                                                                                 tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGCCCCC‑CTTTTTCAATAATGTTTTCGCCTCGCGCTGTAGCCCGCGCCGGCCCCCCCCGCCCCCCCGCGATGCCACGTTGGTATCGCCGTTGATCGCCCTCCGTAGGGGCAAGGGATCGAGCAATGCCAGTTCAggg                                                                                                                                      >  1:182246/1‑249 (MQ=255)
                                                                                                                 tgCTAAAAACCAGGGAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCC‑TTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGa                                                                                                                                      <  2:182246/250‑1 (MQ=255)
                                                                                                                         aCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCC‑TTTTTTCAATAATGTTTTTGCCCCGCGCTGTAGCCAGCGCAGGCCCCCCCCGCCACCCCCCGCTGCAACGTTGGGATCGCCCGTGATCGCCCTCAGTAGGGGCAAGGGATCGGGCAATGCCAGGTCAGGATCTAAcccc                                                                                                                             >  1:116027/1‑251 (MQ=255)
                                                                                                                           cAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAAAGCCCCC‑TTTTTTCAATAATTTTTTCGCCTCCCGCTGTAGCCCACGCAGGGCCCCCCCTCCACCCCGCGGTGCAACGTTGGTGTCGCCGTTGGTCGCCATCAGTAGGAGCAAGGTATCTGGCAATGCCAATTCAGGGTCTAAACCCTg                                                                                                                           >  1:261778/1‑251 (MQ=255)
                                                                                                                                 aGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCC‑TTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCcag                                                                                                                     <  1:267359/251‑1 (MQ=255)
                                                                                                                                  ggATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCC‑TTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCcagc                                                                                                                    <  2:252366/251‑1 (MQ=255)
                                                                                                                                    aTCAGTAGGTCAGCACGTCCGCCTGGACTGAGATTTCGGTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCC‑TTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCcagcag                                                                                                                  <  2:176486/251‑1 (MQ=255)
                                                                                                                                       aGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCC‑TTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGt                                                                                                               <  1:341916/251‑1 (MQ=255)
                                                                                                                                                                 gagaTTGCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCT‑TTTTTGCAATAATGTTTGCGCCTGGCGCTGTAGCCAGCGCAGTCCCCCCGCGCCTCCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGTTATCGAGCAATGCCAGTTGAGGATCTAACCCCTGATCAAGCAGAGTGAGGTAATGCGGCAAGGCGtgatt                                                                                       <  1:240783/249‑1 (MQ=255)
                                                                                                                                                                           tcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCC‑TTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTGAGGTAATGCGGCAAGGCGTGATTGATCACC‑AGTg                                                                           <  1:222382/250‑1 (MQ=255)
                                                                                                                                                                                              aCTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGCCCCC‑CTTTTTCAAAAATGTTTTCGCCCCCCGCTGTAGCCAGCGCCGGCCCCCCCCCCCACCCCGCGGTGCAACGCTGGGATCGCCGTTGGTCGCCCTCCATAAGAGCCAGGGATCGGACAAAGCCCGTTTAGGATCTAACCCCTGATCCCGCCAAGGGGGGGAATGCGGCAAGGCGTGATTGATCACCCGGTGG‑TAACCCGCTTCGGCTTcc                                                       >  1:456552/1‑250 (MQ=255)
                                                                                                                                                                                                                     gcggGGGTTCGAATTCCCCC‑TTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTGAGGTAATGCGGCAAGGCGTGATTGATCACC‑AGTGGATAACCCGCTTCGGCTTCACCGCGTGCGCCGGTAAGGCCAAg                                <  2:482251/251‑1 (MQ=255)
                                                                                                                                                                                                                             tCGAATGACCCC‑TTTTTGCCATAATGTTTGCGCCTCGCGCTGTAGCCCGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGCTCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGCTCTAACCCCTGATCCCGCAGCGTGAGGTAATGCGGCAAGGCGTGATTGACCACC‑AGTGGATAAACCGCTTCGGCTTCACCGCGTGCGCCGGTAAGGCCAAGCTGTGGGt                        >  2:229854/1‑251 (MQ=255)
                                                                                                                                                                                                                                aaTGCCCCC‑TTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTGAGGTAATGCGGCAAGGCGTGATTGATCACC‑AGTGGATAACCCGCTTCGGCTTCACCGCGTGCGCCGGTAAGGCCAAGCTGTTGGTAcc                     >  2:549282/1‑250 (MQ=255)
                                                                                                                                                                                                                                aaTGCCCCC‑TTTTTGCAATAATGTGTGCGCCTCGCGCTGTAGCCAACTCAGGCCCCCCGCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTGAGGTAATGCGGCAAGGCGTGATTGATCACC‑AGTGGATAACCCGCTTCGGCTTCACCGCGTGCGCCGGTAAGGCCAAGCTGTTGGTACa                     <  1:49865/251‑1 (MQ=255)
                                                                                                                                                                                                                                                cAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCACCGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTGAGGTAATGCGGCAAGGCGTGATTGATCACC‑AGTGGATAACCCGCTTCGGCTTCACCGCGGGCGCCGGTAAGGCAAAGCTGTTGGTACAACCGTTGACATGCCg      >  2:328801/1‑251 (MQ=255)
                                                                                                                                                                                                                                                       gTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGCTGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGCGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTGAGGTAATGCGGCAAGGCGTGATTGATCACC‑AGTGGATAACCCGCTTCGGCTTCACCGCGTGCGCCGGTAAGGCCAAGCTGTTGGTACAACCGTTGACCTGCCGTccg  >  2:60809/1‑246 (MQ=255)
                                                                                                                                                                                                                                                       gTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTGAGGTAATGCGGCAAGGCGTGATTGATCACC‑AGTGGATAACCCGCTTCGGCTTCACCGCGTGCGCCGGTAAGGCCAAGCTGTTGGTACAACCGTTGACCTGCCGTCAg  <  1:60809/248‑1 (MQ=255)
                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    
CATCACCACCAGTGGGGCCAATAGTTTCCATATATTATCTTTTGCTAAAGACATACGGGTTCTCCGAAAATTAATATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGCCCCC‑TTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCCCTGATCCAGCAGAGTGAGGTAATGCGGCAAGGCGTGATTGATCACC‑AGTGGATAACCCGCTTCGGCTTCACCGCGTGCGCCGGTAAGGCCAAGCTGTTGGTACAACCGTTGACCTGCCGTCAG  >  NC_000913/646527‑647020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑