Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,111,259	Δ1 bp	coding (718/900 nt)	rfbD ←	dTDP‑4‑dehydrorhamnose reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,111,259	0	G	.	100.0%	134.2 / NA	29	coding (718/900 nt)	rfbD	dTDP‑4‑dehydrorhamnose reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (13/16);  total (13/16)

ATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCT  >  NC_000913/2111026‑2111509                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                           atTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTAATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGCGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGCGTGCAAGGG‑AATGCCTGCTTTgcgcgc                                                                                                                                                                                                                                          >  1:357832/1‑251 (MQ=255)          aaTTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCaaa                                                                                                                                                                                                                                 <  1:38636/251‑1 (MQ=255)           aTTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCaaa                                                                                                                                                                                                                                 >  1:529689/1‑250 (MQ=255)                   tGCTCCACCATCACGAACAATATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACCGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAgc                                                                                                                                                                                                                          <  1:70449/249‑1 (MQ=255)                            aTCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATc                                                                                                                                                                                                               >  1:105438/1‑251 (MQ=255)                                      aGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGtt                                                                                                                                                                                                     >  1:8418/1‑251 (MQ=255)                                              aaaCTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTAcc                                                                                                                                                                                             <  1:238791/251‑1 (MQ=255)                                                      aaaTTGCTGTAGTCGTAAATAATTCATTGAGCAGTCGTTTCACGCCAACCTGCTAGTCATGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTAGTAAGGCGAGAGTTATGTGGACGACGAGCTTGTTTAGGATAGGCTGTTGTTGGTACTGCTTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTa                                                                                                                                                                                     <  1:355615/251‑1 (MQ=255)                                                                                 tGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGGGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATcc                                                                                                                                                           <  2:388280/250‑1 (MQ=255)                                                                                   aGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGt                                                                                                                                                        <  1:438646/251‑1 (MQ=255)                                                                                     cagtCGTTTCACGCCAGCCTGCCAGTCAGGCAAGACCAGAGCAAAGTTCTGCTGAAATTTTTATGTATTACGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCTTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAAGGGTACAAGCCTGCGACCTCCGGttt                                                                                                                                                      <  2:439817/248‑1 (MQ=255)                                                                                               aCGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACGACTGGCTACCAAATTGTACAAGCCTGCGACATCCGGTTTATTCAGTGCg                                                                                                                                            <  2:357832/251‑1 (MQ=255)                                                                                                   cAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGacac                                                                                                                                        <  1:184887/251‑1 (MQ=255)                                                                                                       cTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAAt                                                                                                                                    >  1:210833/1‑251 (MQ=255)                                                                                                                  gCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTg                                                                                                                         <  1:383861/251‑1 (MQ=255)                                                                                                                    aaGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCATCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTa                                                                                                                       <  1:52689/251‑1 (MQ=255)                                                                                                                    aaGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGt                                                                                                                        >  2:220688/1‑250 (MQ=255)                                                                                                                     aGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTAc                                                                                                                      >  2:195267/1‑251 (MQ=255)                                                                                                                             gcAAAGTTCGGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATTTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTTTGGGACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATcagcc                                                                                                              <  2:159208/251‑1 (MQ=255)                                                                                                                                 aGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGCGTTATGTGGACGACGAGCTGATGTAGGATAGGCTGTTGTTGGGACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATcagccagc                                                                                                           >  2:412320/1‑250 (MQ=255)                                                                                                                                          gAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACCCGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCAc                                                                                                 >  2:436605/1‑251 (MQ=255)                                                                                                                                                tttCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTg                                                                                           >  1:251663/1‑251 (MQ=255)                                                                                                                                                            ggCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACt                                                                               >  2:85867/1‑251 (MQ=255)                                                                                                                                                                 gagTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCg                                                                          <  1:338486/251‑1 (MQ=255)                                                                                                                                                                 gagTGATGAGGACGACTAGCTGGTGTAGGAGAGGCTGTTGTTGGTGCTTCGGTGAGCTTGTTGAGTGCAAGGG‑AATGCCTTCTTTGCGCGCCTCATCAAAAACCAGCGCAGCATAAGCGTTCCAGTGTGTGGTACCACTGGCGACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGGATGGCATGTGCTGGACAATCAGCCAGCAGTTCATCACCTGTGGGTGCACCAAACTGATCg                                                                          <  2:341548/251‑1 (MQ=255)                                                                                                                                                                    ttATGTGGACGACGAGCTGGTGTAGGATAGGCTGTGGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAACCCAGCGCAGCATAGGCGTACCAGGTTGTGGTACGACTGGCTACCAAATGGTACAAGCCTGCGTCTTCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTTGCGCAc                                                                                     <  1:317459/237‑1 (MQ=255)                                                                                                                                                                                     tGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAAttcttc                                                      >  2:541196/1‑251 (MQ=255)                                                                                                                                                                                         gtAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACg                                                   <  2:8418/250‑1 (MQ=255)                                                                                                                                                                                                    tgttgtTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGG‑AATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGcc                                         >  2:518692/1‑249 (MQ=255)                                                                                                                                                                                                                                       agggAATGCCTGCTTTGCGAGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTAtttcatttt     >  1:482721/2‑251 (MQ=255)                                                                                                                                                                                                                                          gAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTAtttccttttcct  <  1:195267/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                           ATTCCTTTTAATTCATCTTGCTCCACCATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTGCGTTGAGCTTGTTGAGTGCAAGGGGAATGCCTGCTTTGCGCGCCTCTTCAAAAACCAGCGCAGCATAATCGTACCAGGTTGTGGTACCACTGGCTACCAAATGGTACAAGCCTGCGACATCCGGTTTATTCAGTGCGACACGAATGGCATGTGCTGTACAATCAGCCAGCAGTTCAGCACCTGTTGGCGCACCAAACTGATCGTTAATAACCGCTAATTCTTCACGCTCTTTTGCCAGACGTAACATCGTTTTGGCGAAGTTATTTCCTTTTCCT  >  NC_000913/2111026‑2111509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑