Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,638,058	C→T	L455L (CTG→TTG)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,638,058	0	C	T	100.0%	156.5 / NA	49	L455L (CTG→TTG)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/32);  total (17/32)

TTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCA‑TCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCG  >  NC_000913/4637818‑4638295                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              ttACTGGATAACGCCTTCGATTTTACCCCCGAGAGCGGTTGCA‑TCGCGCTAAGCGCCGAAGTGGATCAGGAACACGTCACTCTTAAGGTGCTTGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATGTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGTCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTTTTTAACGGCGAAGTCACGTTGCGCAAcc                                                                                                                                                                                                                                      <  2:228547/250‑2 (MQ=255)           aCGCCATCGATTTTACCCCCGAGAGCGGTTGCA‑TCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGt                                                                                                                                                                                                                           <  1:127460/251‑1 (MQ=255)            cGCCATCGATTTTACCCCCGAGAGCGGTTGCA‑TCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTg                                                                                                                                                                                                                          <  1:132094/251‑1 (MQ=255)            cGCCATCGATTTTACACCCGAGAGCGGGTGCT‑TCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGCGCTAGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGAGAAGTCACGTTGCGCAACGTGCAGGAAGGTg                                                                                                                                                                                                                          >  2:220716/1‑251 (MQ=255)             gCCATCGAATTTACCCCCGAGAACGGTTGCA‑CCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGCGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGGATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTGAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTgg                                                                                                                                                                                                                         >  1:103510/1‑251 (MQ=255)                aTCGATTTTACCCCCGAGAGCGGTTGCA‑TCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGAGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGGACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTGGCGCAACGTGCAGGGAGGGGGCGt                                                                                                                                                                                                                      >  1:313590/1‑251 (MQ=255)                              cGAGAGCGGTTGCA‑TCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTc                                                                                                                                                                                                        <  1:124148/251‑1 (MQ=255)                              cGAGAGCGGGTGCAATCA‑GCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCTATTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTc                                                                                                                                                                                                        <  2:121003/251‑1 (MQ=255)                                 gagCGGTTGCA‑TCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGGGGCGTGCTGGCCTCGCTTCGAc                                                                                                                                                                                                     <  1:257040/251‑1 (MQ=255)                                  agtggTTGAA‑TCACGTTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTTGATTCCGGTAGTGGTATTCCTGACTACGGGGTTTCACGTATTTGTGAATGCTTTTACTCTTTGCCTCTTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACt                                                                                                                                                                                                    <  1:390220/248‑1 (MQ=255)                                      gTTGCA‑TCAAGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGAAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCAc                                                                                                                                                                                                >  1:306712/1‑251 (MQ=255)                                         gCA‑TCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGt                                                                                                                                                                                             <  1:166862/251‑1 (MQ=255)                                              cACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACt                                                                                                                                                                                         <  1:71090/251‑1 (MQ=255)                                                cGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTc                                                                                                                                                                                       <  1:95829/251‑1 (MQ=255)                                                        gcCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATAACGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCt                                                                                                                                                                               >  1:352681/1‑251 (MQ=255)                                                            aaGTGGATCAGGAACACTTCCCGCTGAAGGGGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCTCGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTAGCTTCGACTTCACCGTCACCTCCCATATCTTCaa                                                                                                                                                                           <  2:256510/251‑1 (MQ=255)                                                                       gAACACGTCACGCTGAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGATTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCTAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCcac                                                                                                                                                                <  2:314908/251‑1 (MQ=255)                                                                         acacGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACAt                                                                                                                                                              >  1:295813/1‑251 (MQ=255)                                                                                   cTTAAGGTACTTGAGACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTACTCTTGAACGCTTTTTCTCTTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGT‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCAACGGTACGTTGCGCAACGTGCAGGAAGCTGGCGTGCTGGCCTCGCTTCGACTTCACCGCCACTTCACATAGCTTCAAATACTTCCCACATAGTCTTCg                                                                                                                                                      <  1:84701/249‑1 (MQ=255)                                                                                    ttAAGGAGCTGGATACCGGTAGTGGTATTCCTGACTAAGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCAACATAGTCTTCGTAt                                                                                                                                                   >  2:286381/1‑251 (MQ=255)                                                                                      aaGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCGTCGTATcc                                                                                                                                                 <  2:295813/251‑1 (MQ=255)                                                                                              ggATACCGGTAGTGGTATGCCTGACTACGCGCGTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGGCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCATGAAGGTGGCGTGCTGGCCTCGCTGCGACTTCACTGTCACGTCACATAGCTTCAAATTCTTCCCGCATAGCCGACGTATCCCGCTGcc                                                                                                                                          <  2:183780/250‑1 (MQ=255)                                                                                                aTACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCAtt                                                                                                                                       <  1:106922/251‑1 (MQ=255)                                                                                                  tccGGTATTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGGCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCTACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTg                                                                                                                                      <  1:397258/249‑1 (MQ=255)                                                                                                  aCCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGc                                                                                                                                     >  2:77947/1‑251 (MQ=255)                                                                                                       tAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAgg                                                                                                                                >  1:109743/1‑251 (MQ=255)                                                                                                                 ccTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTAt                                                                                                                      <  1:359274/251‑1 (MQ=255)                                                                                                                           cgcTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATcc                                                                                                            <  2:157874/251‑1 (MQ=255)                                                                                                                                 cACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCt                                                                                                       <  1:410701/250‑1 (MQ=255)                                                                                                                                      aTTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTCACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGCCTATGTTGAAATCCCCCCTGTTCTg                                                                                                 >  2:351837/1‑251 (MQ=255)                                                                                                                                        ttttGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTgg                                                                                                <  1:142792/250‑1 (MQ=255)                                                                                                                                        tgtttaaCGCTTTTGCTCTTTGCCTCTATCAAATGGGCAAAAATGCAGCGGGCTGGGTGTTG‑CGTTCGTCTGTGAGGTCTCCCGTTTTTTCAACGGCGAAGTCACGTTGCGCAACGTGCAGGAATGTGGCGGGCTGGCCTCGATTCGGCTGCACCGTCACTTCACATAGCTCCAGATTCTGCCTACATAGTCTTCGTATCCTCCCGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTgg                                                                                                <  1:175522/245‑1 (MQ=255)                                                                                                                                          ttGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGACGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTATGGaa                                                                                              >  1:402664/1‑250 (MQ=255)                                                                                                                                                      aCTCGTTGCCGCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTg                                                                                 <  2:314462/251‑1 (MQ=255)                                                                                                                                                           ttGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGtt                                                                               <  1:10641/248‑1 (MQ=255)                                                                                                                                                                 cGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGt                                                                      >  2:132941/1‑251 (MQ=255)                                                                                                                                                                     cAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTg                                                                  >  1:312542/1‑251 (MQ=255)                                                                                                                                                                         tGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGACCGTTTGTTTAAAGGCGAAGTCACGTTGCGCCACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCAACGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCtgt                                                              >  1:265866/1‑251 (MQ=255)                                                                                                                                                                             cAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGGCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCTCATATTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCtgttgtg                                                          <  2:288295/251‑2 (MQ=255)                                                                                                                                                                                aaaaGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTgttgttaat                                                       <  2:87513/251‑4 (MQ=255)                                                                                                                                                                                   agcagcGGTCTGGG‑TTTTGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGTAAGGTGGCGTGCTGGCCTCGCTTCGTCTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTcc                                                    <  1:393306/251‑1 (MQ=255)                                                                                                                                                                                   agcagcGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTGAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCGGGCCTCGCTTCGACGTCACCGTCACTTCACATAGCTTCAAATGCTTCCCACATAGTCTTCGTATCCTGCGGCCATTGCAAAGGAGAAGACTGTGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGGTTGGTGCAGTATTGCGGTGGTTAATTcc                                                    <  2:216635/251‑1 (MQ=255)                                                                                                                                                                                    gcagcGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCAGTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCg                                                   <  1:351837/251‑1 (MQ=255)                                                                                                                                                                                     cagcGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGCAGTCACGTTGCGCAACGTGCCGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCg                                                   >  2:190447/1‑250 (MQ=255)                                                                                                                                                                                            cTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCt                                           >  1:338068/1‑251 (MQ=255)                                                                                                                                                                                              ggg‑gTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTg                                          <  2:5263/250‑1 (MQ=255)                                                                                                                                                                                                                    ggTCGCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGTCTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAAc                    <  1:239402/251‑1 (MQ=255)                                                                                                                                                                                                                        gCCCGTTTGTTTAACGGCGAAGTCACGTTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGc                >  1:14288/1‑251 (MQ=255)                                                                                                                                                                                                                                      cGGCGAAGTCGCGTTGGGCAACGTGCAGGAAGGTGGCGTGCTGTCCTTGGTTCGACGTCACCGTCTCTTCACAAAGCTTCAAATTCTTCCCACATAGTCTTCGTAACCTGTTTCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTAATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGcc  <  2:1410/251‑2 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              TTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCA‑TCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAATGGGCAAAAAAGCAGCGGTCTGGG‑GTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGATTACCGTAGCG  >  NC_000913/4637818‑4638295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑