Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,925	G→C	R77P (CGC→CCC)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,925	0	G	C	100.0%	142.0 / NA	46	R77P (CGC→CCC)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (25/21);  total (25/21)

GCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCTCGCG  >  NC_000913/4636680‑4637171                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       gCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGt                                                                                                                                                                                                                                                   <  1:320702/251‑1 (MQ=255)      aGGGGCCTGTAAGGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTGTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGGTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTcgc                                                                                                                                                                                                                                              <  1:40904/251‑1 (MQ=255)       ggggCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTcgcg                                                                                                                                                                                                                                             <  1:136134/251‑1 (MQ=255)            cTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGGGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGGACCTGGGGCAGGCGTTTAATCCGCTACAACATACCCCGGTTcgcggccat                                                                                                                                                                                                                                        >  2:376011/1‑246 (MQ=255)              gTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAatat                                                                                                                                                                                                                                      <  1:76732/251‑1 (MQ=255)               tAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTCATCAGCTACAACATCCCCCTGTACGCGCCAATATc                                                                                                                                                                                                                                     >  2:82578/1‑251 (MQ=255)                                cGGTTGTTGCTGGGATATTTTTTCCTGGTGGCGGTGGCAGCCTGGTTCGTACTGGGCCTTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCACCGGAGGGGACGTTGCTCGACACCGCAACGGTGCTGGCGGGGCTGGCGCGTCCCGATTTGCTCTCTGGGGGCCCAACGCATGGGCAACTGGCGCAGGCGTTTAATTAGCGACCACATCCCCCGTTTCGCCCCAATATCGGTGGCATTTACAAAGt                                                                                                                                                                                                                    >  2:170881/1‑251 (MQ=255)                                 gtgtttggCTGGGCTATTTTTTACTGGTGGCGGTGGCCGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGTGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACAGCCCCCGTGTCGCGCCAATATCGGTGGCATTACCAAAGTg                                                                                                                                                                                                                   <  2:270821/244‑1 (MQ=255)                                  gttgttGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACAGCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTgc                                                                                                                                                                                                                  <  2:414019/251‑1 (MQ=255)                                     gttgCTGGGCTATTTTTTACTGGTGGCGGGGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGATAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTGCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGAGCAGGCGTTTAATCATCTACAACATCCCCCGTTTCGCGCCAATAACGGTGGCATTAACAAATTgcgc                                                                                                                                                                                                                >  1:148645/1‑250 (MQ=255)                                        gCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATg                                                                                                                                                                                                            <  1:415880/251‑1 (MQ=255)                                                             ggcggtggcAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGCCGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCCGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGActg                                                                                                                                                                                       >  2:291494/1‑249 (MQ=255)                                                                         cTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGc                                                                                                                                                                            >  1:150630/1‑250 (MQ=255)                                                                           ggTTCGTACTGGCCATTTTTTTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGGTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTTCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTTCGCAATGAATATCATGTCTATATGTCCGATGCGCAGGGCaa                                                                                                                                                                          <  2:152771/250‑1 (MQ=255)                                                                           ggTTCGGACTGGCCATTTTTGGTAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGAGCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACAGCCCCCGTTTCGCGCCAATATCGGTGGCATTATCAAAGTGCGCAATGAATATCATGTCTATATGACCGAGGCGCAGGGCa                                                                                                                                                                           <  2:183021/249‑1 (MQ=255)                                                                                gTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTAtt                                                                                                                                                                    <  1:291494/251‑1 (MQ=255)                                                                                          tttttGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACACATGGGAAACTGGCGCAAGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCg                                                                                                                                                          >  1:373191/1‑251 (MQ=255)                                                                                              tGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCCTGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCa                                                                                                                                                       >  1:291548/1‑250 (MQ=255)                                                                                                          tAAACCGTGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACGCCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCGCTCTGGGGTCCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGACTCGGCAAATAAAGCCGtt                                                                                                                                           <  1:80606/250‑1 (MQ=255)                                                                                                           aaaCCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTg                                                                                                                                          <  1:308936/250‑1 (MQ=255)                                                                                                                         aaGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCTTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTTGCATGAGCAAAGTGCGCAATGAATATCATGTCTAGATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTcgc                                                                                                                           <  1:239728/251‑1 (MQ=255)                                                                                                                                     ggggACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTCGCGCGTACCGATTTGCTCCCTGGGGACCCCACGCCTGGGCAACTGGCGCAGGCGATTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCCAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTTTTCGCGCTGGAATGACg                                                                                                               >  2:278055/1‑251 (MQ=255)                                                                                                                                        gACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCt                                                                                                            <  1:357282/251‑1 (MQ=255)                                                                                                                                        gACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCt                                                                                                            <  1:103467/251‑1 (MQ=255)                                                                                                                                        gACGTTGATCGACACCGCAACGTTGCTGGCGGAGCGGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGAGTGGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCt                                                                                                            <  1:178751/251‑1 (MQ=255)                                                                                                                                                  gACACCGCAACGTTGCTGGCGGCGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATACCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGtt                                                                                                  >  1:273917/1‑251 (MQ=255)                                                                                                                                                  gACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGGTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGtt                                                                                                  >  1:120401/1‑251 (MQ=255)                                                                                                                                                   acacCGGAACGTTGATGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGCGACCCACCGCATGGGCCCCAGGCGCAGGCGTTTAATCAGCTACAACAACCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTACGCAATGCATATCATGTCTATATGACCGCTGCGCAGGGCAAAGAATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGGTTATTCGCGCTGGGATGCCTTCTGGCTAACGTTg                                                                                                 >  2:278842/1‑251 (MQ=255)                                                                                                                                                        gCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCACTGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGCCAGGATTATTCGCGCTGGGATGACGTCTGGCTAACGTTGCGTgg                                                                                            >  2:293792/1‑251 (MQ=255)                                                                                                                                                          aaCGTTGGTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAACGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTc                                                                                          >  2:64614/1‑251 (MQ=255)                                                                                                                                                            cGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTc                                                                                          >  1:263072/1‑249 (MQ=255)                                                                                                                                                                             gcgcGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGGCAGTATGGTGCGCGCAGCAc                                                                       <  2:411341/251‑1 (MQ=255)                                                                                                                                                                                 ggcccGATTTGCTCTCGGGGGACCCAACGCATGGGCAACTGGCGCCGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATAGCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTg                                                                   <  2:16872/249‑1 (MQ=255)                                                                                                                                                                                         ttGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATcc                                                           >  1:131658/1‑251 (MQ=255)                                                                                                                                                                                          tGCTCTCTGGAGACCCAACGCATGTGCAACTGGCGCAGGCTTTTAATCAGCTACAACATCCCCCGTTACGCGCCAATATCGGTGTCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCGTTTGTTCAGGATTATTCGCGCTGGAATGACGGCTGGCGAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATcc                                                           <  1:178039/250‑1 (MQ=255)                                                                                                                                                                                           gCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTTCAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACTTCTGGCTAACTTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCt                                                          <  1:124975/250‑1 (MQ=255)                                                                                                                                                                                                 tGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCCAAGTCTTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCCCGTTGCAAAATCCTGCCGATc                                                   >  1:58669/1‑251 (MQ=255)                                                                                                                                                                                                           aCGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTc                                         >  1:81191/1‑251 (MQ=255)                                                                                                                                                                                                               aTGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCtgtg                                     >  2:249300/1‑251 (MQ=255)                                                                                                                                                                                                                ggtgCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCGGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGGTGGACAGGATTATTCGCTCTGGGATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGa                                    <  1:396885/248‑1 (MQ=255)                                                                                                                                                                                                                 gggCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGCAAGTTCTGTGat                                   >  2:255937/1‑251 (MQ=255)                                                                                                                                                                                                                    cAACTGGCGCAGGCGTTTAATCAGCCACAACATACCCCGTTTCGCGCAAATATCGGTGGCATTAACAACGTGCGCAATGAATATCCTGTCTATATGACCGATGCGAAGCGCAAAGTATTGTTCGATTCGGCAAATAACGCCGTTGGACCGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCAAGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGctgt                                 >  2:33209/1‑250 (MQ=255)                                                                                                                                                                                                                                 cGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCAATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCg                   >  1:424241/1‑251 (MQ=255)                                                                                                                                                                                                                                      aaTCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTAt              >  1:242578/1‑251 (MQ=255)                                                                                                                                                                                                                                             tACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGc       >  1:300485/1‑251 (MQ=255)                                                                                                                                                                                                                                                  caTCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCTcgcg  >  1:374801/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       GCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACGGCTCGCG  >  NC_000913/4636680‑4637171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑