Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,630,295 C→T Q76Q (CAG→CAA)  ettA ← energy‑dependent translational throttle protein EttA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,630,2950CT97.9% 158.1 / ‑6.6 47Q76Q (CAG→CAA) ettAenergy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (27/19);  total (27/20)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCG‑GCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTC  >  NC_000913/4630050‑4630537
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   
gcgTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCG‑GCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCgg                                                                                                                                                                                                                                                <  1:155985/251‑1 (MQ=255)
  gTCCCAGTCCGGCAGCCGTAGCGCATCCTCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGGGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCG‑GCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAAGGGACTCACGCACGGTGTGTTCCGGGTGCAGCTGCGGTTCCTGCGGCa                                                                                                                                                                                                                                              <  2:267998/251‑1 (MQ=255)
     ccAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCG‑GCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGAt                                                                                                                                                                                                                                           <  2:118931/251‑1 (MQ=255)
       aGTCCGGCAGACGTCGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCG‑GCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATa                                                                                                                                                                                                                                          >  1:119640/1‑250 (MQ=255)
                          aTCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCG‑GCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCt                                                                                                                                                                                                                     >  1:116485/1‑251 (MQ=255)
                                     gCTCCAGCGTTACGTGCAGTTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCGTGTCAAAATCG‑GCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTGCTGAAACCGCTGCTGCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAAGCTTGATGG‑CTGGCTGCGGATGCGCt                                                                                                                                                                                                          <  2:247190/251‑1 (MQ=255)
                                        ccAGCTGTACGTTCAGATTATGACCGTCGGGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCG‑GCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGGTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTc                                                                                                                                                                                                        <  2:66450/250‑1 (MQ=255)
                                          aGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCG‑GCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAATCCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCAcc                                                                                                                                                                                                     <  1:287452/251‑1 (MQ=255)
                                           gcggTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCT‑GCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCCCTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGTCTGCGGATGCGCTTCAccc                                                                                                                                                                                                    <  2:364285/248‑2 (MQ=255)
                                              gTACGTTCCGATTATGACAGACGTGAGCCTGACTGCTCTCTTCCAGACGGACTTGTTCAGCGCCCAGCTTGTCAAAATCG‑GCATCCGGATCGGCGTACAGCGCCTACACTTCATCCAGGCGTTTCAGGGCGTTACCCACTTCTGAAACCGCTTCTTCAATGGCCTCCCGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑ATGGCTGCGGACGCGCTTCACCTTCg                                                                                                                                                                                                 >  2:358966/1‑251 (MQ=255)
                                              gTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCG‑GCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGGTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCTCCTTc                                                                                                                                                                                                  <  2:215858/250‑1 (MQ=255)
                                              gTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCGTGTCAAAATCG‑GCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCtt                                                                                                                                                                                                   <  2:313639/249‑1 (MQ=255)
                                              gTACGGTCCGATTATGCCCGTCGTAAGCCTGAATGATCTCTTCCATACGGCCTTGTTCAGCGGCCAGCTTATCCAAATCG‑GCATCCGCATCGGCGTACAGCGCATACACTTCATCCAGACGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGCCTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCGGCTAACCCATCTTCATGT‑CTGGCTGCGGACGCGCTGCACCTTCg                                                                                                                                                                                                 >  2:221367/1‑251 (MQ=255)
                                               tACGTTCAGATTATGACCGTCGTGAGCCTGCATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCG‑GCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCgc                                                                                                                                                                                                >  2:66472/1‑250 (MQ=255)
                                                     cAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCG‑GCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCt                                                                                                                                                                                           <  1:4570/250‑1 (MQ=255)
                                                          tATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCG‑GCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGCTAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATc                                                                                                                                                                                      >  1:86893/1‑250 (MQ=255)
                                                                         cctaaaTGATCTCTTCCAGACGGCCTTGTTCGGCGGCCAGCTTGTCAAAATCG‑GCATCGGGATCGGCGTACAGCGCATACACTTCATCCAGGTGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAAGGGACTCACGCAGGGTGTGTTCCGGGTTCAGCTGCGTTTCTTGCGGCAGATAACCATTCTTGATGT‑CGGGCGGCGGACGCGCTTCCCCTTCGATGTTTTTATCAATGCCCGCCATAATg                                                                                                                                                                      <  1:227463/247‑1 (MQ=255)
                                                                                        ccAGACGGCCTTTTTCTGCGGTCAGTTTGT‑AAAAACGTGCTCCCGGATCGCCGTACGTCGAATCCCCTTCACCAAGGGGTTTCAGTGTGTTAACCACTCCGGAAACCGTTTCTTCAAGGGACTCACGCACGGTGTTTTCCGGTTTCAGCTCGGGTTCTTGGGGCATATAACCAAT‑TTGGAGTGCTGGCTGGGGACGCGCTCCTCTTGCGATGTCTTTACCAATGCCCGCCATAATGCGCAGCAGGGTGGa                                                                                                                                                        <  1:216057/250‑1 (MQ=255)
                                                                                              ggCCTTGTTCAGCGGCCAGCTTGTCAAAATCG‑GCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTAccc                                                                                                                                                 >  1:278008/1‑251 (MQ=255)
                                                                                                            gCCAGCTTGTCAAAATCG‑GCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCCTTCAGAcc                                                                                                                                   >  2:95882/1‑251 (MQ=255)
                                                                                                               aGCTTGTCAAAATCG‑GCATCCGGATCGGCGAACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGAccc                                                                                                                                  >  1:371150/1‑249 (MQ=255)
                                                                                                                      cAAAATCG‑GCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCAcc                                                                                                                                                                                                     <  2:956/175‑1 (MQ=255)
                                                                                                                      cAAAATCG‑GCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCAcc                                                                                                                                                                                                     >  1:956/1‑175 (MQ=255)
                                                                                                                          aTCG‑GCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAAttt                                                                                                                     >  2:233155/1‑251 (MQ=255)
                                                                                                                                cATCCGGATCGGCGTACAGCTCATCCACTTCACCCTGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTGCTTCAATGGACTCACGCACGTTGTGTTTCGGTTGCAGCGGCGGTGCTTGCGGCAGATAACCAATCTTGATGT‑CTGTCGGCGGACGCGCTTCACCTGCTATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGcc                                                                                                                <  1:176534/251‑1 (MQ=255)
                                                                                                                                     ggATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGc                                                                                                                                                                                                                                                  >  2:157484/1‑117 (MQ=255)
                                                                                                                                     ggATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGc                                                                                                                                                                                                                                                  <  1:157484/117‑1 (MQ=255)
                                                                                                                                            cGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGgcagaa                                                                                                     >  1:336972/1‑250 (MQ=255)
                                                                                                                                                cagcgctttccctccATCCAGGCTTTTCAGGGCGTCAACCGCTTCTGACCCCTTTTCTGCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGAAAACCAATCTTGATTT‑CGGGTTGCGGACGCGCTTCACCTTCTATGTTTTTATCAATTCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCa                                                                                                <  1:85616/237‑1 (MQ=255)
                                                                                                                                                      aTACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTg                                                                                                                                                          >  1:107703/1‑187 (MQ=255)
                                                                                                                                                               acccAGGCGTTTCAGGGCGTTACCCACTTCTGAAGCCGCTTCTTCAGTGGGCTCACGCAGGGTGTGTTCCGGGTTCAGCTGCGGTTTTTGGGGCAGATAACAAATCTTGGTGT‑CTGGCGGCGGACGCGCTTCCCCTTCGATGTTTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCa                                                                                 <  1:25758/249‑1 (MQ=255)
                                                                                                                                                                     gCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGCCACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAatct                                                                           >  1:154077/1‑249 (MQ=255)
                                                                                                                                                                                  ttAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTcggcgg                                                              >  1:266018/1‑251 (MQ=255)
                                                                                                                                                                                          ttCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACtt                                                      >  2:332066/1‑251 (MQ=255)
                                                                                                                                                                                          ttCTGAAACCGCTTCTTCAACGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCCGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACtt                                                      >  1:350307/1‑251 (MQ=255)
                                                                                                                                                                                           gcggaacccGCTTCTGCAATGGACTCACGCACGGTGGGTTCCGGGTTCAGCGGGGGTTCTGGCGGCAGATAACCAATCTTGATGT‑CTGGCGGCGGCCGCGCTTCACCTTCGTTTTCTTTACAAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACGCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACt                                                       <  1:360614/242‑1 (MQ=255)
                                                                                                                                                                                                   cGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCCGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGCACAACTTTGCCGacac                                             >  2:343167/1‑251 (MQ=255)
                                                                                                                                                                                                    gCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGAGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACg                                            >  1:55155/1‑251 (MQ=255)
                                                                                                                                                                                                         ttcAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGCACAACTTTGCCGACACGATGc                                        >  1:311726/1‑250 (MQ=255)
                                                                                                                                                                                                                gcctcccgcaggGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTTTTTACAAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGtata                                <  1:95882/245‑1 (MQ=255)
                                                                                                                                                                                                                                 ttCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGGGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTAAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGCATAAACGAATTTAGCCACGt               >  1:12145/1‑251 (MQ=255)
                                                                                                                                                                                                                                        ttCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CGGGCTGCGGACGCGCTTCACCTACGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACt                                                                                                                                                      <  1:174895/109‑1 (MQ=255)
                                                                                                                                                                                                                                        ttCAGCTGCGGTTCTTGCGGCAGATAAACAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACt                                                                                                                                                      >  2:174895/1‑109 (MQ=255)
                                                                                                                                                                                                                                           aGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGc     >  1:93601/1‑251 (MQ=255)
                                                                                                                                                                                                                                           aGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAAAGAATTGAGCCACGTTGGACTTCGc     >  1:403842/1‑251 (MQ=255)
                                                                                                                                                                                                                                             cTGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCct   >  1:13343/1‑251 (MQ=255)
                                                                                                                                                                                                                                              tGCGGTTCTTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCctc  >  1:151038/1‑251 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   
GCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCG‑GCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGT‑CTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTC  >  NC_000913/4630050‑4630537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑