Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,604,517	C→T	L120L (CTG→TTG)	bglJ →	DNA‑binding transcriptional regulator BglJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,604,517	0	C	T	100.0%	117.7 / NA	34	L120L (CTG→TTG)	bglJ	DNA‑binding transcriptional regulator BglJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/20);  total (14/20)

ATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCA  >  NC_000913/4604281‑4604762                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTtcat                                                                                                                                                                                                                                         <  1:108735/251‑1 (MQ=255)            gAGTCATTTCAGGATGCCATGGCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTGTCATCAGGAACTCtt                                                                                                                                                                                                                              <  2:95920/251‑1 (MQ=255)              gTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCtttt                                                                                                                                                                                                                            <  1:65397/251‑1 (MQ=255)                 aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTAATTTTTTTTTTTTCTTCCCTGAGAAGGGAGGGCCGAGAGGGAGTTTTTTGCCTGGCTTAAATGGCGGTTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGGTATTTAAGATCGGCTGTTTTGTTTATTGTCGCCATCACCGCTGGGCGGGGTGTTAAGTAAAGCGTCCACGTTGGGGATTTTTTATAATGAACTCTTTTTGt                                                                                                                                                                                                                         >  1:263212/1‑251 (MQ=255)                     cAGGATGCTATGTCGGGGATCTCGTTTGGTGAGGGCATTTTGTCTTTTTCGGCCATGAGAAGTGAGCTCAGAGAGGGATTATCTGGCCTGTCTGAACTGGCGTTTAAGTGTCCGCGTCTCCGGCGTTTATGTATTGCGGATGATGATATGGAAGCGCGGCTGATTGGTGCATTGTCGGCAGCCCCTCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCAGCAGGATCTCTTATTGTCAtt                                                                                                                                                                                                                     <  2:390942/251‑1 (MQ=255)                       ggATGCCATGTCGCGTAGCGCGTTTGCGGCGGTCATTTTTGCGTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCTGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCTCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTGTTCATCTGGAACTCTCTTTGTCATTaa                                                                                                                                                                                                                   <  2:216744/251‑1 (MQ=255)                                                    cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGTATTATCTTGCCTGACTGAACTGGCGATTACGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTg                                                                                                                                                                                      >  1:161134/1‑251 (MQ=255)                                                           tttttctttttcTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTACATGGTGTACGTCAGGCGACCGACCGACTGAACAATc                                                                                                                                                                               >  2:146366/1‑251 (MQ=255)                                                                  ttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGTCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGAGTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTg                                                                                                                                                                           <  1:180359/248‑1 (MQ=255)                                                                  ttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTgg                                                                                                                                                                          <  1:349621/249‑1 (MQ=255)                                                                        gCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACAGACTGAACAATCAGTGGTACATTa                                                                                                                                                                   >  1:330205/1‑250 (MQ=255)                                                                          cATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCCCCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGCACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAAcc                                                                                                                                                                >  2:226358/1‑251 (MQ=255)                                                                                     aGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATAAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACg                                                                                                                                                     <  1:167174/251‑1 (MQ=255)                                                                                          agagagGTATTATCTTGCCTGACTGAACTGGCGCTTAAGTTTCCGCGTACCCGGCGTTTAGATATTGCGGATGATGATATTGACGATCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGAGTCAACGTTGGAGCTTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCCAAGCCGGACGTTa                                                                                                                                                  >  2:144759/1‑249 (MQ=255)                                                                                              agGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCg                                                                                                                                            >  1:214841/1‑251 (MQ=255)                                                                                              agGGATTATCTGGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCg                                                                                                                                            <  2:214841/251‑1 (MQ=255)                                                                                                          gCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGgagagaga                                                                                                                                 <  1:249839/250‑1 (MQ=255)                                                                                                          gCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATGGGTTCATTGTCGCCATCACCGCTGGACGTTGTATTAAGTAAAGCTTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTGGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGTTACATTAACCAATGCCGGACGTTAAGGCCGACGGAGAGAGaa                                                                                                                                <  2:374132/251‑1 (MQ=255)                                                                                                                 tgaACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCTACGTTGGAGCTTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTgc                                                                                                                         >  1:288356/1‑251 (MQ=255)                                                                                                                  gaACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTgc                                                                                                                         <  1:7453/250‑1 (MQ=255)                                                                                                                         cGATTAAGTTTCCGCTGACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAATGGTTCATTGTCGCCATCTCCGCTGGACGGTGTATTAAGTAAAGCGTCAACTTTGGTGATTTTTCATCTGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGATCGACCGACTGAACAATCAGTGGTACATGAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGGCATAGGGCGCTTTATg                                                                                                                 <  2:160589/251‑1 (MQ=255)                                                                                                                                  ttCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGc                                                                                                        >  1:199086/1‑251 (MQ=255)                                                                                                                                                            gCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGGGGCTTCTCAATGACACAAATTGCCGagca                                                                              <  2:199086/251‑1 (MQ=255)                                                                                                                                                                 tgatgatCTTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCACCGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCtt                                                                          >  2:59852/1‑250 (MQ=255)                                                                                                                                                                      atatTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGc                                                                    <  1:248920/251‑1 (MQ=255)                                                                                                                                                                           gAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAata                                                                <  1:226358/250‑1 (MQ=255)                                                                                                                                                                                      gATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGGCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGTGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGGCACAAATGGCTGAGCATCTTAAAGGCAATATGAAAACGAt                                                      <  2:210060/249‑1 (MQ=255)                                                                                                                                                                                                        aTCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACg                                                                     >  1:182801/1‑216 (MQ=255)                                                                                                                                                                                                        aTCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTGATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACg                                                                     <  2:182801/216‑1 (MQ=255)                                                                                                                                                                                                            ccGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCGTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGAt                              <  1:21237/251‑1 (MQ=255)                                                                                                                                                                                                                          aTTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCg                >  1:91367/1‑251 (MQ=255)                                                                                                                                                                                                                             aaGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGCACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTc              >  1:93652/1‑250 (MQ=255)                                                                                                                                                                                                                                        cAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGc   >  2:236029/1‑250 (MQ=255)                                                                                                                                                                                                                                        cAACGTTGGAGATGTTTCATCAGGAGCTCTGTGTGTCAGTAAATGGTGTACGTCAGGCGACCGAATGACCTAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCAGAGCAGCGTAAACTCAATATCAAAACGTTCCGTGCACATAAATGTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGcc  <  2:146252/251‑2 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      ATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCA  >  NC_000913/4604281‑4604762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑