Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,585,673	C→T	W363* (TGG→TGA)	hsdR ←	type I restriction enzyme EcoKI endonuclease subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,585,673	0	C	T	100.0%	174.3 / NA	49	W363* (TGG→TGA)	hsdR	type I restriction enzyme EcoKI endonuclease subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/23);  total (26/23)

CAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATCCGCAAAGCCCTGATTACCCGTTTCATCTTTTCCGGTCGGCCATTCG  >  NC_000913/4585433‑4585916                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    cAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGttt                                                                                                                                                                                                                                           >  1:273169/1‑251 (MQ=255)      cgctcATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCGTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATAc                                                                                                                                                                                                                                      <  1:207296/251‑1 (MQ=255)               cAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACt                                                                                                                                                                                                                             <  1:210267/251‑1 (MQ=255)                                        cagTTTTTACGGTTCGCTGCCGAGCATTTCAAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGTAATCACGCCCGTCGCGAGACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATACGCGCCCGTTGGTCGCGTAGCAGAAGGGGTTTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGATTCATACTCTGGCACTTCTTACTGCACTTCATCCGGTGagg                                                                                                                                                                                                    >  2:238694/3‑250 (MQ=255)                                             tttGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGc                                                                                                                                                                                               >  1:85199/1‑251 (MQ=255)                                                 cGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGCTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTca                                                                                                                                                                                            >  1:410197/1‑250 (MQ=255)                                                        cTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTCTCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTACACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCCCTTCATCCGGTGAGTAGTGCTCAAGCAAGGt                                                                                                                                                                                    >  2:299422/1‑251 (MQ=255)                                                              aGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCg                                                                                                                                                                              >  2:226906/1‑251 (MQ=255)                                                                       agcagcTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCTCAGGAAGcc                                                                                                                                                                     <  2:242231/251‑1 (MQ=255)                                                                          agcTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCAtt                                                                                                                                                                  <  2:139974/251‑1 (MQ=255)                                                                           gcTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCAtat                                                                                                                                                                 >  2:43174/1‑249 (MQ=255)                                                                           gcTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACATCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTa                                                                                                                                                                 <  1:101817/251‑1 (MQ=255)                                                                           gcTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTGCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTGCCCGCAGGAAGCCATTa                                                                                                                                                                 <  2:198774/251‑1 (MQ=255)                                                                              ctTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCATGAAGCCATTAt                                                                                                                                                                <  2:370265/249‑1 (MQ=255)                                                                                   gggCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAAc                                                                                                                                                          >  2:100899/1‑250 (MQ=255)                                                                                    ggCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATATCAGATGCCGCTTTTGGTCTTCATTGGTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACAt                                                                                                                                                        <  1:100550/251‑1 (MQ=255)                                                                                        gTGCCACTCGGGTAAGGCTTTCGACAGATTGCGGGTATCACGCACGTCGCGATACCTGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCATCTGGTTGCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTAc                                                                                                                                                    <  1:157460/251‑1 (MQ=255)                                                                                             aCTCGGGGAAGGCTTTCAACATATTGCGGGTATCACGCACGTCGCGATACAAGAGGCCGCTTCTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCAGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGACGCCATTATCGAAACATTTACTGt                                                                                                                                                 >  2:65349/1‑249 (MQ=255)                                                                                                   gTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATAc                                                                                                                                         >  1:329338/1‑251 (MQ=255)                                                                                                            tCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCAtt                                                                                                                                 <  2:108753/250‑1 (MQ=255)                                                                                                              gACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTg                                                                                                                                >  2:355058/1‑249 (MQ=255)                                                                                                               aCATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCTGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGANACGACTCATTGAGc                                                                                                                             <  1:70306/251‑1 (MQ=255)                                                                                                               aCATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGc                                                                                                                             <  1:340343/251‑1 (MQ=255)                                                                                                                        gggTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGACGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCggg                                                                                                                     >  2:87414/1‑250 (MQ=255)                                                                                                                         ggTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGa                                                                                                                    >  2:41322/1‑250 (MQ=255)                                                                                                                          gTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAAc                                                                                                                  <  1:100899/251‑1 (MQ=255)                                                                                                                                          gATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGGAGCAGAAGGGGATTTTAAACCGGTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGTACGTCGATATTGTTACGt                                                                                                  >  2:116218/1‑251 (MQ=255)                                                                                                                                             aCCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATATTGTTACGttt                                                                                                <  2:158570/250‑1 (MQ=255)                                                                                                                                                    gCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATATTGTTACGTTTCGCctct                                                                                         >  1:379801/1‑250 (MQ=255)                                                                                                                                                      cGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTc                                                                                                                                    >  1:255168/1‑205 (MQ=255)                                                                                                                                                      cGCTTTTGGTCTTCAGTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTc                                                                                                                                    <  2:255168/205‑1 (MQ=255)                                                                                                                                                          tttGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACcg                                                                                  >  2:109021/1‑251 (MQ=255)                                                                                                                                                           ttGGTCTTCATTGTTGCTCGGTATGCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGTTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACcg                                                                                  <  2:5684/250‑1 (MQ=255)                                                                                                                                                             ggTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACcgcg                                                                                >  2:159267/1‑250 (MQ=255)                                                                                                                                                                 ttCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATggg                                                                           >  2:179490/1‑251 (MQ=255)                                                                                                                                                                     ttgttgCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCAACGCGATGGGTTTg                                                                       >  1:276057/1‑251 (MQ=255)                                                                                                                                                                      tgttgCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTg                                                                       >  1:176836/1‑250 (MQ=255)                                                                                                                                                                       gttgCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAg                                                                     <  1:226906/251‑1 (MQ=255)                                                                                                                                                                                        cccGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGa                                                                                                                    >  1:102572/1‑187 (MQ=255)                                                                                                                                                                                        cccGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGa                                                                                                                    <  2:102572/187‑1 (MQ=255)                                                                                                                                                                                               gTCGAGTAGCAGAAGGGGATTTTAAACCGGTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAAAGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATcctc                                             >  2:68553/1‑249 (MQ=255)                                                                                                                                                                                                        cAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATCCGCAAAGCCCTg                                    >  1:95216/1‑251 (MQ=255)                                                                                                                                                                                                         aGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATCCGCAAAGCCCTGa                                   <  2:128681/251‑1 (MQ=255)                                                                                                                                                                                                          gAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATATTGTTACGTGTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATCCGCAAAGCCCTGAt                                  <  2:352177/251‑1 (MQ=255)                                                                                                                                                                                                                      aaaCCGTTGTTTGCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATCCGCAAAGCCCTGATTACCCGTTTCa                       <  1:362067/250‑1 (MQ=255)                                                                                                                                                                                                                                  gCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCgg                                                                                                                      <  1:10055/143‑1 (MQ=255)                                                                                                                                                                                                                                  gCCGCTGGTGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCgg                                                                                                                      >  2:10055/1‑143 (MQ=255)                                                                                                                                                                                                                                   ccGCTGGGGTCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCCCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATCCGCAAAGCCCTGATTACCCGTTTCATCTTTTCCGGTCgg         <  2:329338/251‑1 (MQ=255)                                                                                                                                                                                                                                          tgtCCTGTCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATCCGCAAAGCCCTGATTACCCGTTTCATCTTTTCCGGTCGGCCATTCg  >  2:360916/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    CAGCTCGCTCATGCCAGGGTTATCGGCAAACCACTGATTCTGTTTTTGCGGTTCGCTGCCGAGCATTTCCAGCAGCTCTTCCGGGCGGTGCCACTCGGGTAAGGCTTTCGACATATTGCGGGTATCACGCACGTCGCGATACCAGATGCCGCTTTTGGTCTTCATTGTTGCGCGGTATTCGCGCCCGTTGGTCGAGTAGCAGAAGGGGATTTTAAACCGTTGTTTGCCGCTGGTGTCCTGCCAGCTGGTTTCATACTCTGGCACTGCTTCATGCACTTCATCCGGTGAGTAGTGCTCAAGCAAGGTTTCCCGCAGGAAGCCATTATCGAAACATTTACTGTAGCGATACGACTCATTGAGCCTGGCGGGAACGTCGATATTGTTACGTTTCGCCTCTACCACCGCGATGGGTTTGAGGCCGACAAACAGCACATAATCCGCAAAGCCCTGATTACCCGTTTCATCTTTTCCGGTCGGCCATTCG  >  NC_000913/4585433‑4585916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑