Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,182,791	A→T	D516V (GAC→GTC)	rpoB →	RNA polymerase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,182,791	0	A	T	100.0%	198.7 / NA	63	D516V (GAC→GTC)	rpoB	RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (26/37);  total (26/37)

GATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTG  >  NC_000913/4182544‑4183036                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      gATATCCGTAACGGTAAAGGCGAAGGCTATGATATCGACCACCTCGGCAACCGGCGTTTCCGTGCCGTTGGCGAAATGGCGGAAAACCAGTGCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGAGTTCCGCAGCAGTGAAAGATTTCTTCGGGTCCAGCCAGCTGTCTTAGTTTATGGTCCa                                                                                                                                                                                                                                                    <  2:276678/251‑1 (MQ=255)    atCCGTAACGGTAAAGGCGAAGTCGATGATTTCGGCCACGGCGGCAACCGTTGTATCCGGTCCTGTGGCGAAATGGCGGAAAACCAGTTCCTCGGTTTCCTGGTACGCGTAGAGCGTGCGTTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATGTCCTGATGCCACAGGATATGAGCAACTCCAAGCCTAGTTCCGCAGCAGTGAAAGAGGGCTTCGGTGCCAGCCGGCTTTCTCAGTTCATTGTCCAGaa                                                                                                                                                                                                                                                 <  2:44209/251‑1 (MQ=255)        gTAACGGTAAAGGCGAAGTCGATGATATCTACCACCGCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGaacaac                                                                                                                                                                                                                                             <  1:294819/251‑1 (MQ=255)             ggTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCt                                                                                                                                                                                                                                        <  1:263614/251‑1 (MQ=255)                aaaGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTGCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGt                                                                                                                                                                                                                                      <  1:242750/250‑1 (MQ=255)                                atatCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGACTCAGTTTATGGTCCAGAACAACCCGCTGTCTgagcatacgcccaa                                                                                                                                                                                                                      >  2:93596/1‑239 (MQ=255)                                      cccaccTCGGCAACCGTCGTAGCCGGTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAAcgtcgt                                                                                                                                                                                                               <  1:286854/250‑1 (MQ=255)                                                 aaCCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAACACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGAGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGTTTCCAGCCAGATGACTCTGTTTATGGTCCAGAACAAACCGCTGTCTGAGATTAAGCCCAAACGTCGTATCTACGCAc                                                                                                                                                                                                     >  2:3830/1‑250 (MQ=255)                                                 aaCCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCAcc                                                                                                                                                                                                    >  2:219329/1‑250 (MQ=255)                                                          aTCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATTCCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCTCTCGTCCCAg                                                                                                                                                                                            <  1:322746/250‑1 (MQ=255)                                                                 ccgttGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTg                                                                                                                                                                                    >  1:186766/1‑251 (MQ=255)                                                                  cgttGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGCGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTg                                                                                                                                                                                    >  1:27423/1‑250 (MQ=255)                                                                   gttGGGGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGGGTAGAGCGTGCGGTGAAAGAGCGACTGTCTCTGGGCGATCTGGATACCCTGATGCAACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGCATACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGGCTGAc                                                                                                                                                                                  >  1:284207/1‑251 (MQ=255)                                                                           aaTGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCCCTCGGCCCAGGCGGGCTGACCCGTGAAc                                                                                                                                                                          >  2:337686/1‑251 (MQ=255)                                                                            aTGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCTCACTCGGCCCAGGCGGTCTGCCCCGTGAACg                                                                                                                                                                         >  2:192529/1‑251 (MQ=255)                                                                                     aaCCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCtt                                                                                                                                                                <  1:198362/251‑1 (MQ=255)                                                                                       ccAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGCTATGATAAACGCCAAGCCGATTTCCGCAGCAGTGCAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCg                                                                                                                                                              >  1:376856/1‑251 (MQ=255)                                                                                            tCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGtt                                                                                                                                                         >  1:250642/1‑251 (MQ=255)                                                                                                cgTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCg                                                                                                                                                       >  1:32264/1‑249 (MQ=255)                                                                                                 gTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCg                                                                                                                                                       >  2:368813/1‑248 (MQ=255)                                                                                                   tGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGTGAc                                                                                                                                                   <  2:355018/250‑1 (MQ=255)                                                                                                   tGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGAc                                                                                                                                                   <  2:67606/250‑1 (MQ=255)                                                                                                    ggCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCAACAGGATATGATCAACGCCAAGCCGATTTCCGAAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTCTGGTCCAGAACAACCCGCTGTCTGTGATTACGCACAAACGTCGTATCTCCTCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCTAAGTTCGTGACGt                                                                                                                                                 >  1:408727/1‑251 (MQ=255)                                                                                                      ccTGGTACGTGTAGATCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGGGCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCTAAGTGCGAGACGTac                                                                                                                                               <  2:61478/251‑1 (MQ=255)                                                                                                        tGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTTTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTCTGGTACAGAACCACCCGCTGTCTGAGATTACGCACAACCGTCGTATCTCCGCCCTCGGACCAGGCGGTCAGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAAACgaccac                                                                                                                                             >  2:256252/1‑246 (MQ=255)                                                                                                         ggTACGTGGAGAGCGTGCGGTGAAAGAGCGTCTGGCTCTGGTCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTacac                                                                                                                                             <  1:256252/250‑1 (MQ=255)                                                                                                                gtAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTc                                                                                                                                      <  2:309721/250‑1 (MQ=255)                                                                                                                    agCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTAc                                                                                                                                 <  1:337686/251‑1 (MQ=255)                                                                                                                    agCGTGCGGTGAAAGAGCGTCTGTCGCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTGCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCGGTCTCAGTTTATGGTGCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCTCTCGGCCCAGGCGGTCTGTCCCTTTATTTTGCAGGCTTCGAAGTGCGTGACGTACTCCTTACTCACTAc                                                                                                                                 <  2:401663/251‑1 (MQ=255)                                                                                                                              gAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCAACTCACTACGGTCGCGTAt                                                                                                                       <  2:38293/251‑1 (MQ=255)                                                                                                                                  gagCGTCTGTCTCTGGGCGATCTGGATCCCCTGATGCCACAGGAGATGATCAACGCCAAGCCGATTTCCGCAGGAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCGCAAACGTCGTATCTCCGCACTCGGCGCAGGCGGTCTGACCCGTGAACGTGCAGGCTGCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTcc                                                                                                                   <  1:395304/251‑1 (MQ=255)                                                                                                                                  gagCGTCTGGCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTGCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTcc                                                                                                                   <  2:132852/251‑1 (MQ=255)                                                                                                                                      gtctgtctCTGGGCGATCTGGATACCCTGATGCCACAGGATAGGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGGATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATc                                                                                                               <  1:293375/251‑1 (MQ=255)                                                                                                                                       tctgtctCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCATGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCg                                                                                                              >  2:111118/1‑251 (MQ=255)                                                                                                                                       tctgtctCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGATTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCg                                                                                                              >  1:265460/1‑251 (MQ=255)                                                                                                                                              ctGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCTAAAc                                                                                                          <  1:121412/248‑1 (MQ=255)                                                                                                                                              cagggCGATCGGGATACCCTGGTGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTAATGTCCAGAACAACCCGCTGTCTGAGATTACGCCCAAACGTCGTATCTCCGCACTCGGCCCAGGCTGTCTGACCCGTTAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAAcc                                                                                                         <  2:141952/247‑1 (MQ=255)                                                                                                                                                gggCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTg                                                                                                     <  2:262160/251‑1 (MQ=255)                                                                                                                                                 ggCGATCTGGATACCATGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGa                                                                                                    <  1:136321/251‑1 (MQ=255)                                                                                                                                                         ggatgccatGATTCCACAGGATATGATCTACGTACAGCCGTTGTCGGCAGCAGTGAAAGAGTTCTTCGATTCCAGCCAGCCTTCTCAGTTTATGTTTCAGAACAACCCGCTGTCTAATATTACTCACAAACGTCGTAACGCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTGCGATGTTTGAAACGTACACCCGACTCACTACGGTCGCGTCTGTCCAATCGAACCACCTGATGGTc                                                                                               <  2:236285/240‑1 (MQ=255)                                                                                                                                                                 tGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGt                                                                                    >  2:182482/1‑251 (MQ=255)                                                                                                                                                                   aTGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGCACATCGGTCt                                                                                  >  2:30404/1‑251 (MQ=255)                                                                                                                                                                                 gATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGt                                                                    <  1:368813/251‑1 (MQ=255)                                                                                                                                                                                  aTCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTGCTTCTGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACGCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACGCCCGACTCACTACGGTCGCGTATGTCCAAGCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTc                                                                   <  1:36310/251‑1 (MQ=255)                                                                                                                                                                                         ccAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGTAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTAc                                                            <  1:71655/251‑1 (MQ=255)                                                                                                                                                                                         ccAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAAGCGAATCCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTAc                                                            <  1:213945/251‑1 (MQ=255)                                                                                                                                                                                                          gcagTGAAGGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACCAACGTCGTATCTCCGCACTCGACCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGGAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCCCAGACTAACGAAt                                            >  2:72530/1‑250 (MQ=255)                                                                                                                                                                                                             gggAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACgg                                        <  2:267122/249‑1 (MQ=255)                                                                                                                                                                                                              tGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGc                                       >  1:208864/1‑251 (MQ=255)                                                                                                                                                                                                                   gagTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGGGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGCATCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCt                                  >  2:270229/1‑251 (MQ=255)                                                                                                                                                                                                                    agTTCTTCGGTTCCAGCCAACTGTCTCAGTTTATGGTACAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTACGCACTCGGCCACGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACCTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATAGGTCTGATCAACTCTCTGTCCGTGATCGCACAGACTAACGCATCCGGCTTCCtt                                 >  2:218608/1‑251 (MQ=255)                                                                                                                                                                                                                       tcttcGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTg                                >  2:256562/1‑249 (MQ=255)                                                                                                                                                                                                                          tcGGTTCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGAc                            <  1:415492/250‑1 (MQ=255)                                                                                                                                                                                                                               tCCAGCCAGCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGt                       <  1:276115/250‑1 (MQ=255)                                                                                                                                                                                                                                ccagccagCTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTa                      <  1:322649/250‑1 (MQ=255)                                                                                                                                                                                                                                  agccagcTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGa                                                                                            <  1:347420/178‑1 (MQ=255)                                                                                                                                                                                                                                  agccagcTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGa                                                                                            >  2:347420/1‑178 (MQ=255)                                                                                                                                                                                                                                       gcTGTCTCAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTaa                >  1:282588/1‑249 (MQ=255)                                                                                                                                                                                                                                              cAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGAc          <  1:350512/248‑1 (MQ=255)                                                                                                                                                                                                                                              cAGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGAGCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGAcc         <  1:256562/249‑1 (MQ=255)                                                                                                                                                                                                                                               aGTTTATGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGAc      <  1:30404/251‑1 (MQ=255)                                                                                                                                                                                                                                                   tATGGTCCAGAACAACCCGCTGTCTGAGATTTCGCACAAAAGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGATTCCTTGAGCCTCCGTATCGTAAAGTGACCGACgggg  >  2:243027/1‑249 (MQ=255)                                                                                                                                                                                                                                                    aTGGTCCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGgtg  <  1:231792/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      GATATCCGTAACGGTAAAGGCGAAGTCGATGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGTTGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTGTAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTG  >  NC_000913/4182544‑4183036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑