Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,533,518	C→T	S234F (TCC→TTC)	pck →	phosphoenolpyruvate carboxykinase (ATP)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,533,518	0	C	T	100.0%	169.7 / NA	52	S234F (TCC→TTC)	pck	phosphoenolpyruvate carboxykinase (ATP)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/15);  total (37/15)

TTCGCCCGAGCGATGAAGAACTGGCAGGTTTCAAACCAGACTTTATCGTTATGAACGGCGCGAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTCCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAAAGAAGCGGAACCTGAAATCTACAACGCTATCCGTCGTGATGCGTTGCTGGAAAACGTCACCGTG  >  NC_000913/3533275‑3533762                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     ttCGCCCGAGCGATGAAGAACTGGCAGGTTTCAAACCAGACTTTATCGTTATGAACGGCGCGAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAAc                                                                                                                                                                                                                                               >  1:60485/1‑251 (MQ=255)            gATGAAGAACTGGCAGGTTTCAAACCAGACTTTATCGTTATGAACGGCGCGAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGaa                                                                                                                                                                                                                                    <  1:308105/251‑1 (MQ=255)            gATGAAGAACTGGCAGGTTTCAAACCAGACTTTATCGTTATGAACGGCGCAAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGAGGCGCAATGAAGAAAGGGATGTTCTCGATGATGAACTACCCGCGGCCGCTGAAAAGTAGCGCCTCTATGCACTGCTTCGCCAACGtgtggcaga                                                                                                                                                                                                                                     >  2:371595/1‑242 (MQ=255)                aagaaCTGGCAGGTTTCAAACCAGACTTTATCGTTATGAACGGCGCGAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGc                                                                                                                                                                                                                                >  1:194944/1‑251 (MQ=255)                          aggtttaaacgcagccTTTATCGTTATGAACGGCGCGAAGTGCACTAACCCGCCGTGGTAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGCTTGGCGGCACCTGGTACGGCGGCGAACTGAAGCAAGGGATGTTCTCGCTGATGACCTACCTGCTGCCCCTGCAAGGTATCACGTCTATGCCCCGCTTCGGCCCCGTTGGGGAGAAAGGCGGTGTTGCgg                                                                                                                                                                                                                      >  2:202282/16‑251 (MQ=255)                          aGGTTTCAAACCAGACTTTATCGGTATGAACGGCGCGAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCGATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGACGTTGCgg                                                                                                                                                                                                                      <  2:31609/251‑1 (MQ=255)                                cAAACCATACTTTATCGTTATGAACGGCGCGAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGttct                                                                                                                                                                                                                <  2:310791/251‑1 (MQ=255)                                 aaaCCTGACTTTATCGTTATGAACGGCGCGAAGTGCCCTAACTCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCGGCCGCTGAAAGGTATCGCTTCTATGTACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGttctt                                                                                                                                                                                                               <  2:46987/251‑1 (MQ=255)                                             aTCGTTATGAACGGCGCGAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGACAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGGATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCgc                                                                                                                                                                                                   >  1:35260/1‑250 (MQ=255)                                               cGTTATGAACGGCGCGAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGACAGGGATGTTCTCGATGATGAACTACCTGATGCCGCTGAAAAGTATCGCTTCTATGTCCTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTTTTCGGCCTTTCCGGcc                                                                                                                                                                                                 >  2:119747/1‑250 (MQ=255)                                                    tGAACGGCGCGAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGGTGAACTACCTGCTGCCGCTGGAAGGTATCGCTTCTTTGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTTTTCGGCCTTTCCGGCACCggg                                                                                                                                                                                            >  2:300878/1‑250 (MQ=255)                                                                      cTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTAGTGGCGTGTAACCTGACCGAGAGCATGCAGCTGATTGGCGGCACATGGTACGGCGACAAAATGGAGAAAGGGATGTTATCGGTGATGAAATACCTGCTGCCGCTGCAAGGTATCGCTTCTATGAACTGCGTCGCCAACGCTGGTGAGGAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCCTCGGTAAAACCACCCTTTccccc                                                                                                                                                                          >  1:205254/1‑248 (MQ=255)                                                                                 tGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCCCCGGGAAAACCACCCTTTCCACCGACCCGAAACg                                                                                                                                                               >  2:303574/1‑251 (MQ=255)                                                                                                ctcAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCg                                                                                                                                                            <  1:72132/239‑1 (MQ=255)                                                                                                ctcAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGAAAAACCACCCTTTCCACCGACCCGAAACGTCg                                                                                                                                                            >  2:72132/1‑239 (MQ=255)                                                                                                  cAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGAt                                                                                                                                               <  1:138815/250‑1 (MQ=255)                                                                                                            aaCTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACgg                                                                                                                                    <  1:303574/251‑1 (MQ=255)                                                                                                                            aCCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGc                                                                                                                    >  1:322326/1‑251 (MQ=255)                                                                                                                               tGACCGCGCGCATGCAAATGCTTGGAGGCACCTGGTCCGGCGGCGAAATGAAGAAACGGATGTTCTCGATGATGAAACACCTGCTGCCGCTGAAAGGTCTCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGCTATTCGGCCTTTCAGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGaagacggcggcggg                                                                                                                 >  2:395500/1‑238 (MQ=255)                                                                                                                                         cATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGGTGACGGCGTGTTTAACTTCg                                                                                                       >  1:312063/1‑251 (MQ=255)                                                                                                                                             cAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAgg                                                                                                   <  1:348948/251‑1 (MQ=255)                                                                                                                                                            aCCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCaa                                                                                     >  1:232825/1‑250 (MQ=255)                                                                                                                                                             ccTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGGATCGCTTCTATCCCCTGTTTCGCCCACGATGGTGAGAAAGGGGACGTCGCGGTGTTCTTCGGCCTTTCCGGCACCGGAAAAACAACCCTTTCCCCCCACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTTGAAGGGGgctggtgagcaaa                                                                                    >  1:421511/1‑241 (MQ=255)                                                                                                                                                                       cggcgAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGc                                                                         >  1:137045/1‑251 (MQ=255)                                                                                                                                                                        ggcgAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCt                                                                        >  1:16952/1‑251 (MQ=255)                                                                                                                                                                         gcgAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCGGCGGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCTCCGACCCGAAACGTCGCCTGATTGGCGAGGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTg                                                                       <  2:243695/251‑1 (MQ=255)                                                                                                                                                                         gcgAAATGAAGAAAGGGATGTTCGCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTACCAAGCTg                                                                       <  2:60485/251‑1 (MQ=255)                                                                                                                                                                            aaaTGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTTCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCGGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACGATCAAGCTGTCg                                                                    <  2:402074/251‑1 (MQ=255)                                                                                                                                                                               tGAAGAAAGGGATGTTCTCGATGATGATCTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGaa                                                                  >  1:319795/1‑250 (MQ=255)                                                                                                                                                                               tGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGaa                                                                  >  2:344545/1‑250 (MQ=255)                                                                                                                                                                                 aagaaAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGGGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGaa                                                                  >  1:265818/1‑248 (MQ=255)                                                                                                                                                                                  ataaaGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCATGATTGGCGATGACGACCACGGCTGGGACGATGACGGCGCGTTTAACTTCGAAGGCGGCTGCTACGTAAAAACTATCAAGCTGTCGAaaga                                                               >  1:331978/3‑250 (MQ=255)                                                                                                                                                                                  agaaAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAaaga                                                               >  1:331968/1‑250 (MQ=255)                                                                                                                                                                                   gaaAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAaaga                                                               >  1:240975/1‑249 (MQ=255)                                                                                                                                                                                        ggATGTTCTCGCTGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAACACCACCCTTTCCACCGACCCGAAACGTCGCCTGCTTGGCGATGACGAACCCGGCTGGGACGATGACGGCGTGTTTAACTTCGCCGGCGGCTGCTACGCAAAAACTATCACGCTGTCGAAAGAAGCggc                                                         >  2:221260/1‑249 (MQ=255)                                                                                                                                                                                            gTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCCCTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAAAGAAGCGGAACCt                                                     <  2:35260/250‑1 (MQ=255)                                                                                                                                                                                             ttCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAAAGAAGCGGAACCTg                                                    >  1:105399/1‑250 (MQ=255)                                                                                                                                                                                              tctcGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAAAGAAGCGGAACCTGa                                                   >  1:107598/1‑250 (MQ=255)                                                                                                                                                                                               ctcGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAAAGAAGCGGAACATGaa                                                  >  2:237382/1‑250 (MQ=255)                                                                                                                                                                                                    tgatgaACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCCCTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGAACCGAAACGTCGCCTGATTGACGATGACGACCACGGCTGGGACGATGACGGCGTGTTTTACTTCGAAGGCGGCTGCTACGCCAAAACTATCAAGCTGTCGAAAGAAGCGGAACCTGAAATccac                                            >  1:35957/1‑248 (MQ=255)                                                                                                                                                                                                    tgatgaACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGAGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAACCGTCGCCTGCTTGGCGCTGACGAACCCGGCTGGGACGATGCCGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGATGTCGAAAGAAGCGGAACCTGAAATCTAc                                            >  1:179855/1‑251 (MQ=255)                                                                                                                                                                                                      atgaACTACCTGCTGCCGCTGAAAGGTATCGCTTCTCTGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAACACTATCAAGATGTCGAAAGAAGCGGAACCTGAAATCTACa                                           >  2:130938/1‑250 (MQ=255)                                                                                                                                                                                                         aaCTACCAGCTGCAGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGCCGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGGCGAAAGAAGCGGAACCTGAAATCTACAACGc                                       >  2:31821/1‑251 (MQ=255)                                                                                                                                                                                                           cTACCAGCTGCCGCTGAAAGGTATCGATTCTATGCACTGCTTCGCCAACGTTGGTGAGCAAGGCGATGATGCGGTGTTCTGCGGCCTTTCCGGCACCGGTAAAACAACCATTTCCACCGACCCGAAACGTCGACTGATTGGCGGTGACGAACAAGGCGGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCGAACAATCTCCAGCTGTCGACAGAAGGGGCACCTGAAAGCTACAAAGCt                                      >  2:415087/1‑250 (MQ=255)                                                                                                                                                                                                                 gctgcCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAAAGAAGCGGAACCTGAAATCTCCAACGCTCTCcgtc                               >  2:187677/1‑251 (MQ=255)                                                                                                                                                                                                                 gctgcCGCTGAAAGGTATCGCTTCTATGCACTGCGTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGTCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAAAGAAGCGGAACCTGAAATCTACAACGCTATCcgt                                <  1:424625/250‑1 (MQ=255)                                                                                                                                                                                                                           aaaGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAAAGAAGCGGAACCTGAAATCTACAACGCTATCCGTCGTGATGCGtt                     >  1:304290/1‑251 (MQ=255)                                                                                                                                                                                                                                 aTCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGCTGACGGCGTGTTTAACTTCGACGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAAAGAAGCGGAACCTGAAATCTACAACGCTATCCGACGTGATGCGGTGCTgg                >  1:298425/1‑250 (MQ=255)                                                                                                                                                                                                                                   cGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGCAGGCGGCTGCTAAGCAAAAAATATCAAGCTGTCGAAAGAAGCGGAACCTGAAATCTACAACGCTATCCGTCGTGATGCGTTGCTGGaa              >  1:276138/1‑250 (MQ=255)                                                                                                                                                                                                                                   cGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTGCCGGCACCGGTAAAACCACCCGTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAAAGAAGCGGAACCTGAAATCTACAACGCTATCCGTCGGGATTCGTTGCTGGaa              <  2:66708/250‑1 (MQ=255)                                                                                                                                                                                                                                         tATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAAAGAAGCGGAACCTGAAATCTACAACGCTATCCGTCGTGATGCGTTGCTGGAAAACGTc        >  2:378911/1‑250 (MQ=255)                                                                                                                                                                                                                                              aCTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAAAGAAGCGGAACCTGAAATCTACAACGCTATCCGTCGTGATGCGTTGCTGGAAAACGTCACcgtg  <  1:284080/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     TTCGCCCGAGCGATGAAGAACTGGCAGGTTTCAAACCAGACTTTATCGTTATGAACGGCGCGAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTCCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACCGGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAAAGAAGCGGAACCTGAAATCTACAACGCTATCCGTCGTGATGCGTTGCTGGAAAACGTCACCGTG  >  NC_000913/3533275‑3533762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑