Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,804,133	C→T	S211F (TCT→TTT)	nrdF →	ribonucleoside‑diphosphate reductase 2 subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,804,133	0	C	T	100.0%	88.9 / NA	25	S211F (TCT→TTT)	nrdF	ribonucleoside‑diphosphate reductase 2 subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/16);  total (9/16)

TCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAA  >  NC_000913/2803906‑2804379                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       tCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAACAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGACGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGCAAAGATATTTCTGGGAAAACGtgaagagttg                                                                                                                                                                                                                                  >  2:47846/1‑250 (MQ=255)  cAGATTATTCAGCAACAGTATCGCGGTTATGATCCGCGGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGtgaagagttga                                                                                                                                                                                                                                 <  2:106948/250‑1 (MQ=255)   aGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGtgaagagttga                                                                                                                                                                                                                                 >  1:42496/1‑249 (MQ=255)            aGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTGCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACTGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGcc                                                                                                                                                                                                                      <  1:405651/251‑1 (MQ=255)               aaCATTATCGCGGTGATGATACGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTc                                                                                                                                                                                                                   >  1:353111/1‑251 (MQ=255)                        gcgGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTGCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATttgttg                                                                                                                                                                                                          <  1:244233/251‑1 (MQ=255)                             gattattcGCTGAAAAAGAAAAGCGCCAGTGTGTTTCTTGAATCTTTTTGGTTCTATTCCGTTTTCGGGCTGTCGATGTATTTTTCCAGCCGCTGAAAGCTGACCAATACCGCGGACCTTATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACGACATAGGCTAGAAATATCAGAAAAACAGGGAAAAGATATTTCTTGGACAACGTGAAGAGGTGAAGAGTTTCGCCGTCGTTtagttgttgta                                                                                                                                                                                                     <  2:254961/244‑6 (MQ=255)                                                         gtgtgtTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACAcc                                                                                                                                                                         <  2:81265/251‑1 (MQ=255)                                                             gtTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATg                                                                                                                                                                     <  2:353111/251‑1 (MQ=255)                                                                   tGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCt                                                                                                                                                                 <  2:279835/249‑1 (MQ=255)                                                                          tttttGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCg                                                                                                                                                         <  2:312022/250‑1 (MQ=255)                                                                              tGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAAc                                                                                                                                                     <  1:275750/250‑1 (MQ=255)                                                                                                     ccGATGTATTTTTCCAGCCGCAGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGACGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAACACATGGAAAAGCTATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGACCTCTACGACAACGAGTTGCAATACACCGATGCGCTGTACGCCGCAACCCCGTGGGCTGACGATGTGaactc                                                                                                                             >  2:352214/1‑248 (MQ=255)                                                                                                        aTGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGttt                                                                                                                                                                                                                          >  2:232624/1‑155 (MQ=255)                                                                                                        aTGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGttt                                                                                                                                                                                                                          <  1:232624/155‑1 (MQ=255)                                                                                                           tATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTtct                                                                                                                       >  2:176597/1‑251 (MQ=255)                                                                                                                     gCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTTATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATAGCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAAc                                                                                                             <  1:265055/251‑1 (MQ=255)                                                                                                                                  gACCAAGACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCGATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCTATTTGTTGCTGGATCTCGACGACAACGAGTTGCAATACGCCGAGGAGCTGTACGTCGAAACTGCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCtt                                                                                                <  1:49678/251‑1 (MQ=255)                                                                                                                                       aTACCGCGGACCTGATACGTCTGATTATCCGCGATGCAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCACTACACCGATGAGCTGTACGCCGCAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCACTAAGGCTTtgctt                                                                                           >  2:19271/1‑248 (MQ=255)                                                                                                                                                                   ccGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTccc                                                                <  1:17224/250‑1 (MQ=255)                                                                                                                                                                    cgcgATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGc                                                              <  2:253382/251‑1 (MQ=255)                                                                                                                                                                                              cATAGGCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCaa                                    <  1:403648/251‑1 (MQ=255)                                                                                                                                                                                                   gCTATAAATATCAGAAAAACATGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTc                               >  1:129799/1‑251 (MQ=255)                                                                                                                                                                                                                       aTGGAAAAGATATTTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGc           >  1:294538/1‑251 (MQ=255)                                                                                                                                                                                                                                atatTTCTGGGACAACGTGAAGAGTTGAAGAGGTTCGCCTTCGAGTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCGGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGGCGATGaaaa  <  2:42496/251‑1 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       TCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACATAGGCTATAAATATCAGAAAAACATGGAAAAGATATCTCTGGGACAACGTGAAGAGTTGAAGAGTTTCGCCTTCGATTTGTTGCTGGAACTCTACGACAACGAGTTGCAATACACCGATGAGCTGTACGCCGAAACCCCGTGGGCTGACGATGTGAAAGCGTTTCTCTGTTACAACGCCAATAAGGCTTTGATGAATCTGGGCTACGAACCGTTATTTCCCGCAGAAATGGCGGAAGTGAATCCGGCAATCCTCGCCGCGCTTTCGCCGAATGCCGATGAAAA  >  NC_000913/2803906‑2804379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑