Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,507,867	G→A	E363K (GAG→AAG)	rlhA →	23S rRNA 5‑hydroxycytidine C2501 synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,507,867	0	G	A	100.0%	77.5 / NA	23	E363K (GAG→AAG)	rlhA	23S rRNA 5‑hydroxycytidine C2501 synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/10);  total (13/10)

TTCCATCGACGGAAAAGACTTTCCACCGTGGTAGCACAGATTATTTTGTGAATGCCCGTAAAGGCGATATTGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACTGGGCGGCTGGCAGGAACAACTGATTCTGACCCTCACCAGTGAAGAGGGTGTCAGCATCACGCATACGCTGGACGG  >  NC_000913/1507634‑1508105                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                               ttCCATCGACGGAAAAGACTTTCCACCGTGGTAGCACAGATTATTTTGTGAATGCCCGTAAAGGCGATATTGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGGTTAAACGGGCAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAAt                                                                                                                                                                                                                               >  2:293546/1‑251 (MQ=255)     aTCGACGGAAAAGACTTTCCACCGTGGTAGCACAGCTTATTTTGTGAATGCCCGTAAAGGCGCTATTGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGt                                                                                                                                                                                                                           >  2:49135/1‑251 (MQ=255)                          ccgtggtagccataattattTTGTGAAGGCCCGTAAAGGCGATTTTGGCGCGTTAGATGCGCCAAAATTTAGCGGCCTGCCGGTAGGCGAAGTAGTGAAAGGGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGaaa                                                                                                                                                                                                      <  2:324161/237‑1 (MQ=255)                            gTGGTAGCACAGATTATTTTGTGAATGCCCGTAAAGGCGTTATGGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATg                                                                                                                                                                                                    <  1:157640/251‑1 (MQ=255)                                  gCACAGATTATTTTGTGAATGCCCGTAAAGGCGATATGGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGGGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCcagca                                                                                                                                                                                              <  1:412892/251‑1 (MQ=255)                                        attattTTGTGAATGCCCGTAAAGGCGATATTGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGGGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTg                                                                                                                                                                                        <  1:2440/251‑1 (MQ=255)                                                  gAATGCCCGTAAAGGCGATATTGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTc                                                                                                                                                                              >  1:48783/1‑251 (MQ=255)                                                       cccGTAATGGCTATATTGGCGCGTTCTATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCca                                                                                                                                                                         <  1:7466/251‑1 (MQ=255)                                                              aGGCGATATTGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCAcc                                                                                                                                                                  >  1:388795/1‑251 (MQ=255)                                                                                         gAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTACGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAg                                                                                                                                                                                                                            <  1:336158/166‑1 (MQ=255)                                                                                         gAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTACGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAg                                                                                                                                                                                                                            >  2:336158/1‑166 (MQ=255)                                                                                                   ggCCTGCCGGTAGGAAAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTACACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAACCAGTAATCTTGATCATAACTGGCAGCAGGc                                                                                                                             >  1:263948/1‑251 (MQ=255)                                                                                                       tGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTg                                                                                                                         >  2:363799/1‑251 (MQ=255)                                                                                                              aGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCCGCAGGCACTGACaaaa                                                                                                                   >  1:15654/1‑250 (MQ=255)                                                                                                                                               cGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGACGCTTTTCGTGCCAATACGGTCACGAACACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAACTGACAGCAGATTTGCCCAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGACCGTCGGGTGGCGGTAGACATTg                                                                                 >  1:27041/1‑251 (MQ=255)                                                                                                                                                   gTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACt                                                                             >  1:52677/1‑251 (MQ=255)                                                                                                                                                      gCCGTTGCCGAGCCCTTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATTCGGTCAAGAAAACCGGAGAAAATCAGTTCCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCATAAAATTCGTCCACATCACCCCCTAAACCGTAGTCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACTgg                                                                           <  1:250114/250‑1 (MQ=255)                                                                                                                                                                aGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCTGGTGGCGGTAGACATTGAACGGGGCGGCTGGCAg                                                                <  1:83911/251‑1 (MQ=255)                                                                                                                                                                            aTGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACTGGGCGGCTGGCAGGAACAACTGAtt                                                    >  2:102154/1‑251 (MQ=255)                                                                                                                                                                              ggccaTGGCCTGAACGTGTTGATTAAACGTGTATGCGGCGGATTTCGTGCCAATACGGGGAAGAAAACCGTAGAAATTCAGTATCGCGTCTGGCCCAGTGAAATGGCAGCAGATTTGCATAAATGCCGTCCACGTCACCGATTAATTCGGAATCTTGTTCATAACTGGCAGCAGGCACCGACGAAGACCTCCAGCGAATGTCTTGTGGCGGTATACATTGAACTGGGCGGCTGGCAGGAACAACTGATTCt                                                  <  1:253957/247‑1 (MQ=255)                                                                                                                                                                                     gCCTGAACGTGTTGGTTAAACATGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTCCCGCGTCTGGACCAATGAAATGCCAGCAGATTTGCACAAAATTCGCCCACATCACCCACTAAACCGTCATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGACCTGGGCGGCTGGCAGGAACAACTGATTCTGACCCTc                                           >  2:169755/1‑251 (MQ=255)                                                                                                                                                                                                                           cGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAAGTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACTGGGCGGCTGGCAGGAACAACTGATTCTGACCCTCACCAGTGAAGAGGGTGTCAGCATCACGCATACGCTgg      <  1:186058/250‑1 (MQ=255)                                                                                                                                                                                                                              gCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACTGGGCGGCTGGCAGGAACAACTGATTCTGACCCTCACCAGTGAAGAGGGTGTCAGCATCACGCATACGCTGGACgg  >  1:51281/1‑251 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                               TTCCATCGACGGAAAAGACTTTCCACCGTGGTAGCACAGATTATTTTGTGAATGCCCGTAAAGGCGATATTGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACTGGGCGGCTGGCAGGAACAACTGATTCTGACCCTCACCAGTGAAGAGGGTGTCAGCATCACGCATACGCTGGACGG  >  NC_000913/1507634‑1508105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑