Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,301,998	A→G	S273G (AGC→GGC)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,301,998	0	A	G	100.0%	51.7 / NA	17	S273G (AGC→GGC)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/10);  total (7/10)

CACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCC‑GGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCG  >  NC_000913/1301753‑1302232                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            cACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGCCCCAGCCTGGTAATATCGTCACCAACGGTGCATATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGCAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGAATAACGGCATg                                                                                                                                                                                                                                        >  1:249678/1‑251 (MQ=255)            aaaGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGAATAACGGCATGCCGATCGAAtt                                                                                                                                                                                                                             <  2:103834/251‑1 (MQ=255)               gCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGAATAACGGCATGCCGATCGAATTGtt                                                                                                                                                                                                                          <  1:124138/251‑1 (MQ=255)                             atgcGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGaaaa                                                                                                                                                                                                            <  1:348820/248‑1 (MQ=255)                                       aaaTGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATccc                                                                                                                                                                                                  >  1:197885/1‑251 (MQ=255)                                                     tGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCC‑GGACGAAGTTCACg                                                                                                                                                                                   >  1:99351/1‑251 (MQ=255)                                                       gTAATAGCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCC‑GGACGAAGTTCACGtt                                                                                                                                                                                 <  2:191036/251‑1 (MQ=255)                                                                                                       acgaacgaATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCC‑GGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCg                                                                                                                                 >  1:165855/1‑251 (MQ=255)                                                                                                                       tGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCC‑GGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtg                                                                                                                  <  1:11207/250‑1 (MQ=255)                                                                                                                          aCGCAGCCCGACCTACTGGAAAAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCC‑GGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtg                                                                                                              <  1:352499/251‑1 (MQ=255)                                                                                                                                        aCTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCC‑GGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCCTTCACCGCAGTGCGTGTGCGTACCGCACTg                                                                                                >  2:309808/1‑251 (MQ=255)                                                                                                                                                                     cAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCC‑GGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGCATGGACCGCGATATCATTGt                                                                   >  1:397820/1‑251 (MQ=255)                                                                                                                                                                                             tCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCC‑GGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCa                                            <  1:192709/250‑1 (MQ=255)                                                                                                                                                                                               agAAGTAACCGAGGTCAACCGCTACCGTAGTG‑TGTAAATGGACATGACGAATAACGGCAGGCCTATCGAATTGTTCCAGAAGCGGAAAAAAGAGATCCC‑GTACGAAGTTCACGTTGATCCATACCTGTCCTCTGACTATTACAAAATAAACACCCAAAAGCCGCCATGCATCGATGTGCGTGTGCGTACCGCACTGAAAGTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGGGCAGGGCAACa                                         <  1:40886/250‑1 (MQ=255)                                                                                                                                                                                                  aGTTAACGATGTCAACCGCTACCGTAGTGGTG‑AACTCGACATGACGAATACCGGCATGCCGGTCGCATTGTTCCAGAAGCTGCAAAAAGAGATCCC‑GGACGAAGTTCACGATGATCAATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGGGGGCGTACAGAACTGAAACTAGGAATGGACCGCGATATCATTGTTAATAACGTGAAAGCGCAGGGCAACATGc                                      >  2:402196/1‑251 (MQ=255)                                                                                                                                                                                                     tACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGGCGAATAGCGGCATGCCGATCGAATTGTCCCAGAAGCTGAAAAAAGAGATCCC‑GGACGAAGTTCACGTTGATCCATACCTTTGCACTTACTATTACGAAATGAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGAGATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGccc                                    <  2:266583/250‑1 (MQ=255)                                                                                                                                                                                                                                       caaCATGACGAAAACCGGCAGGCCGATGGATTTGTCCCAAAAG‑TTAAAAAAAAAATCCCCGGACAAAGTTCACTTTGATCCATACCGGTGCACTTATTATTCCGAAATAAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGCCCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGgcg  <  2:99351/249‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            CACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG‑AAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCC‑GGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCG  >  NC_000913/1301753‑1302232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑