Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (+589/+167)	rtcR → / ← glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	156.9 / NA	45	intergenic (+589/+167)	rtcR/glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (24/21);  total (24/21)

ATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATG‑CGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGG‑TGCCGATATCGATAAACAGCG‑TCGAGCCATT‑GGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAC  >  NC_000913/3560218‑3560682                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                    ataaaggtgtTAGCAACTTTGAGATTGTTGGTGACAAGG‑GGCAAATTGCTGGGTGTGAGCAGTGCGTGCGCTCCCGT‑TTCGGGGGTGG‑TGCCGATATCGATAAACGGCG‑GCGAGCCATT‑GGGGTTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGTT‑CTGGGTGGCCTTG‑GTATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGtt                                                                                                                                                                                                                         <  1:281693/245‑1 (MQ=255)  tCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATG‑CGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGG‑TGCCGATATCGATAAACAGCG‑TCGAGCCATT‑GGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGttt                                                                                                                                                                                                                        >  1:99107/1‑251 (MQ=255)    acgggggtaccaagtttGAGATTGTGGGTGA‑AATGGCGCAATTTGCTGTATTTGGGAAGGGCGGGCGCTCCCGT‑TTCGGGGGTGG‑GGCCAATTTCGATAACCAGCG‑TCGAGCCATG‑GGGGATTTGCCCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTg                                                                                                                                                                                                                      <  2:141873/236‑1 (MQ=255)            aGCAACGTTGAGATTGTTGGTGACAATG‑CGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGG‑TGCCGATATCGATAAACAGCG‑TCGAGCCATT‑GGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGcc                                                                                                                                                                                                               >  1:184317/1‑250 (MQ=255)                aCGTTGAGATTGTTGGTGACAATG‑CGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGG‑TGCCGATATCGATAAACAGCG‑TCGAGCCATT‑GGGGCTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGATTTGATCCGCCAGCTc                                                                                                                                                                                                          >  2:82988/1‑251 (MQ=255)                   ttGAGATTGTTGGTGACAATG‑CGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGG‑TGCCGATATCGATAAACAGCG‑TCGAGCCATT‑GGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt                                                                                                                                                                                                       >  2:184495/1‑251 (MQ=255)                   tagggttggttgGTGACAATG‑CGAAATTTGTTGGGTTTGAGAGGGGTGTGCGCT‑CCGCCTTCCGGCGTGG‑GGCCAATAACGATAA‑CAACGGTGGGTCCATG‑GGGGTTTTGCCCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CGGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAt                                                                                                                                                                                                        <  2:165199/242‑1 (MQ=255)                      gaaattgtgggggaaaatgccaaaaTTGCGGTGATTGAGCAGTGCGTGCGCTCCCGC‑TTCGGGCGTGG‑TGCCGATATCGATAACCAGCG‑TCGAGCCATT‑GGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTg                                                                                                                                                                                                      <  1:246037/228‑1 (MQ=255)                        aTTGTTGGTGACAATG‑CGCACATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGG‑TGCCGATATCGATAAACAGCG‑TCGAGCCATT‑GGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTGGCTCCGCCAGCTCATTGGGGt                                                                                                                                                                                                  >  2:28682/1‑251 (MQ=255)                         ttttggggtaaaaattcgcAAATGGTTGTGATTGAGCAGTGCGTGCGATCCCGC‑TTCCG‑CGTGGGTACCTATAGGGATAAACAGCG‑TCGAGCCATG‑GGGGTTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTGCGGG‑CTGGGTGGC‑TTGTCGAGCGTGCCTCGGGGTGTTAACCGATCGGGAAGGAAGCGCGGCATCGCCATGATGGCGCATGATCAGGTTTTGCTCCGTCAGCTCATTGAgg                                                                                                                                                                                                   <  1:275301/234‑1 (MQ=255)                                   cAATG‑CGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGG‑TGCCGATATCGATAAACAGCG‑TCGAGCCATT‑GGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAt                                                                                                                                                                                        >  1:132555/1‑250 (MQ=255)                                        gcgaaagttgcgtggATTGAGCAGTGCGCTCTGTACCTT‑TTCCGGCGTGG‑GGCGGATACCGATAATCAGCG‑TCTAGCCATT‑GGGGATTTGCCCCGCCACTTTGCGTGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTATCTGAACTGAAAGGCAGCTCCTCACCGCCATGATGGCGCAGGATCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAggct                                                                                                                                                                                   <  1:275817/237‑4 (MQ=255)                                             aaTTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGG‑TGCCGATATCGATAAACAGCG‑TCGAGCCATT‑GGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCggg                                                                                                                                                                              >  1:91635/1‑251 (MQ=255)                                              attgtggggATTGAGCGGGGGGTGCGCTCCCGC‑TTCGGGGGGGG‑GGCTGATATTGATAAACAGCG‑TCGAGCCATT‑GGGGTTTGGCCCCGCCCCTTTGCGGGCGATGCGCTCTTTTTCTGCGGT‑CGGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGc                                                                                                                                                                             <  2:228044/243‑1 (MQ=255)                                                gccggtgATGGAGGAGTGCGTGCTCTCCCGC‑TCCCGGCGTGG‑GGCCGAAACCGATAAACAGCG‑TCGACCCTTT‑GGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTg                                                                                                                                                                           <  1:73166/247‑1 (MQ=255)                                                  cTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGG‑TGCCGATATCGATAAACAGCG‑TCGAGCCATT‑GGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGAc                                                                                                                                                                         >  1:3976/1‑251 (MQ=255)                                                    gtgATTGAGCATTGCGTGCGCTCCCGC‑TTCCGGCGTGG‑TGCCGATATCGAAAAACAGCG‑TCGAGCCATT‑GGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACgg                                                                                                                                                                       <  1:94143/251‑1 (MQ=255)                                                    gtgATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGG‑TGCCGATATCGATAAACAGCG‑TCGAGCCATT‑GGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACgg                                                                                                                                                                       >  2:94143/1‑251 (MQ=255)                                                             gcagtcggtgcttgctcgctccctgCGTGG‑TGCAGATCTCAACAAACAGCG‑TC‑AACCATTTGGGGTTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGc                                                                                                                                                               <  1:222646/227‑1 (MQ=255)                                                                         tACCGC‑TTCCGGCGTGG‑TGCCGGTATCGATAAAAAGCG‑TCGAGCCATT‑GGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                                                                                                                                  >  2:381506/1‑251 (MQ=255)                                                                         aaCCGC‑TTGCGGCGAGG‑TGCCGATAGCGATAAACAGCG‑TCGGTGCATT‑GGGTATTTGCTCCGCCACTTGGCGGGGTAGGCGCGGTTTTGTTTCGGG‑CTGGGGGGCCTGG‑CGGTCGTGCCACTGCGGGTTAACCGACCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                                                                                                                                  <  1:166775/250‑1 (MQ=255)                                                                          cccGC‑TTCGGGCGTGG‑CGCCGATATCGAGAAACAGCG‑TCGAGCCATT‑GGGGTTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctct                                                                                                                                                   <  2:93995/248‑1 (MQ=255)                                                                                        tgg‑tgCCGATATCGATAAACAGCG‑TCGAGCCATT‑GGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCCTTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGCCATa                                                                                                                                     >  2:316564/1‑250 (MQ=255)                                                                                             ccgtattgcaacaaaCCGCG‑TCGAGCTTTT‑GGGTTTTGGCTCCGCCGTTTTGCGGGGTATGCGCTTTTTTTCTT‑GGTGCTGGGGGGCTTGG‑CATTCGGCCCACGGCGTTTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                                                                                                                <  2:222349/239‑1 (MQ=255)                                                                                                gATATCGATAAACAGCG‑TCGAGCCATT‑GGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGCGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgct                                                                                                                             >  1:116025/1‑251 (MQ=255)                                                                                                                g‑TCGAGCCATT‑GGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGat                                                                                                             >  1:103047/1‑251 (MQ=255)                                                                                                                       cctgtgggggATTTGCCCCGCCGCGTTGCGGGCGATGCTCTCTTTTTCTTCGGG‑CTGGGTGGCCTGG‑CGACCGGGCCCCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAc                                                                                                        <  2:310555/245‑1 (MQ=255)                                                                                                                       ccATT‑GGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGCTCGTGCCACGGCGTGTTAACCGCACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTGGCTCCGCCAGCTCATTGAGGTAGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAGCCTGCTGTTTAACCAGTTCGATAATAcc                                                                                                       >  2:370978/1‑251 (MQ=255)                                                                                                                           tggggttttttccccGCCACTTTGCGGGCGATGCGCTTTTTTCCTTCGGT‑CGGGGTGGCCTTG‑CGATCGGGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGt                                                                                                     <  2:132555/237‑1 (MQ=255)                                                                                                                                     gCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCTGCGAATAGTCTGCGGGCTGCCGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATACTACCGTTGTGACGTtgt                                                                                          >  1:390165/1‑251 (MQ=255)                                                                                                                                     gCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtgt                                                                                          >  2:320270/1‑251 (MQ=255)                                                                                                                                               gtggCGGGCGATGGCCTCTTTTTCTTCGGT‑TTGGGTGGCCTGG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCa                                                                                <  2:99107/248‑1 (MQ=255)                                                                                                                                                ttGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCa                                                                                <  1:19100/250‑1 (MQ=255)                                                                                                                                                        gATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGAAATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAAt                                                                       >  1:57507/1‑251 (MQ=255)                                                                                                                                                              ctctTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtgtg                                                                                         >  2:302244/1‑227 (MQ=255)                                                                                                                                                              ctctTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtgtg                                                                                         <  1:302244/227‑1 (MQ=255)                                                                                                                                                                   tttCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGACTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGAAGTTGTGTTTGTTTCATTTATAAATCCCTGGAatt                                                             >  1:382210/1‑250 (MQ=255)                                                                                                                                                                      cttcGGT‑CTGGGTGGCCTTG‑CGATCGTGTCACGGCGTGTTAACCGACCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGCATTAttt                                                         >  2:153111/1‑251 (MQ=255)                                                                                                                                                                      cttcGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGCACTGGACGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCCGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTTTAACTCCCTGGAATTCttt                                                         >  2:203857/1‑251 (MQ=255)                                                                                                                                                                                   tGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCgcgc                                             >  2:76232/1‑251 (MQ=255)                                                                                                                                                                                     gCCTTG‑CGATCGTGCCACGGCGTGTTAACCGACCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCAt                                           >  2:147513/1‑251 (MQ=255)                                                                                                                                                                                                     cACGGCTTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGTATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTGTCGTTTTCGCGCATTGAGCGAATcaacaa                            <  1:304050/251‑1 (MQ=255)                                                                                                                                                                                                                         ggAAGTCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACg        >  2:410096/1‑251 (MQ=255)                                                                                                                                                                                                                               gagggccgcTCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCGCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATGGAGCGAATCAACAAAAGGCATCGCTAAACCCACGGCTAAc  <  2:72277/247‑1 (MQ=255)                                                                                                                                                                                                                               caccgccgcACCTCCATGATGGCGCAGGATCAGGTGTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACTGAGAAATGCTCTACCAGCTCTTCGGGACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATGTATAAAGCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAGCCCACGGCTAAc  <  2:328982/248‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                    ATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATG‑CGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGC‑TTCCGGCGTGG‑TGCCGATATCGATAAACAGCG‑TCGAGCCATT‑GGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGT‑CTGGGTGGCCTTG‑CGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAC  >  NC_000913/3560218‑3560682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑