Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,395,743	T→C	G53G (GGT→GGC)	tsaE →	N(6)‑L‑threonylcarbamoyladenine synthase, TsaE subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,395,743	0	T	C	100.0%	206.6 / NA	64	G53G (GGT→GGC)	tsaE	N(6)‑L‑threonylcarbamoyladenine synthase, TsaE subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/37);  total (27/37)

GCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGATTATTTTGCCAACGATGCCATCTGCCTGGTGGAGTGGCCACAACAAGGTACAGGTGTTCTTCCTGACCCGGATGTCGAAATACACATTGATTATCAGGCACAAGGCCGTGAG  >  NC_000913/4395497‑4395986                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    gcgcGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTCATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGccatc                                                                                                                                                                                                                                                 <  1:53579/251‑1 (MQ=255)     gTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAggg                                                                                                                                                                                                                                             <  1:499656/251‑1 (MQ=255)        ttGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTa                                                                                                                                                                                                                                           <  1:55355/250‑1 (MQ=255)         tGGAACGCGCTGGATGCTGGCAACCGATCTCTATTCCACGCTACAGCGTATTGTTAACCCTGAAGTGACTGATAAAAACCATGATGAAGCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCTATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACATGCTCTGGGCCATCAGTGTaa                                                                                                                                                                                                                                          <  2:337923/250‑1 (MQ=255)          ggAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAAt                                                                                                                                                                                                                                         <  1:75728/250‑1 (MQ=255)             aCGCGCGGCATGCTAGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAAACCGCAAATGACTGATAAAAACCATGATGAAGCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGATCGGGAAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCCCCTTTAACCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTactttc                                                                                                                                                                                                                                      >  2:278038/1‑245 (MQ=255)                cgcgGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCaaaa                                                                                                                                                                                                                                  <  1:223332/251‑1 (MQ=255)                        gCTGGCAACCGATCTCTTGGCCACGCTACAGCGTATTGTTAACCCTGAAGTGACTGATAAAAACCATGATGAATCGAGGAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACt                                                                                                                                                                                                                          <  2:330034/251‑1 (MQ=255)                           tgCAAACGATCACTTTTCCACTCTACAGCGTATTGTTAACCCGGCAGTGACTGATAAAACCCATGATGAATCGAGTAATTCAGCTCCCTGTCGAGCAGGCAACATTAGACATGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGAAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGATAAAACAAACATTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGAAATGTCAAAAGCCCCGCTtat                                                                                                                                                                                                                       >  2:46066/2‑251 (MQ=255)                             cAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATAc                                                                                                                                                                                                                     <  2:336195/251‑1 (MQ=255)                                ccGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGAAATGTCAAAAGCCCCACTTATACGCt                                                                                                                                                                                                                  >  2:586296/1‑251 (MQ=255)                                       ctTTTCCACGCTACATCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTGTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTg                                                                                                                                                                                                                 <  1:396669/245‑1 (MQ=255)                                       ctTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTgg                                                                                                                                                                                                                <  1:586296/246‑1 (MQ=255)                                              aCGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCtata                                                                                                                                                                                                    <  1:274767/251‑1 (MQ=255)                                                gCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACg                                                                                                                                                                                                  >  1:289328/1‑251 (MQ=255)                                                    cAGCGGATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGGAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGcacg                                                                                                                                                                                              <  2:175895/251‑4 (MQ=255)                                                       cGTAATGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACa                                                                                                                                                                                           <  2:255653/251‑1 (MQ=255)                                                                aaCCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATgg                                                                                                                                                                                  <  2:101597/251‑1 (MQ=255)                                                                      gAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATc                                                                                                                                                                            >  1:4909/1‑251 (MQ=255)                                                                       aaGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTATTTACAGGCTCTAGGCCATCAGGGTAAGGTCAAATGCTCCGCTTATACGCTGTTCGGAACCTATActcacataaacgtaatgatctata                                                                                                                                                                            >  2:135373/1‑227 (MQ=255)                                                                          ggACTGATAAAAACCATGATGAAGCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCGGGGCGAGCGGGTAGCGAAAGCCGGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCTCCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCACCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTAGCACtt                                                                                                                                                                        <  2:256223/250‑1 (MQ=255)                                                                            aCTGATAAAAACCATGATGAAGCGGGTAATTCCGCTGCGTGATGAGCAGGCAACATTAGACCGGGGCGGGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCTCTTATACGCTGGTCGAACCGTTTACTCTCGACATCTTAATGGTTTATCACTTTg                                                                                                                                                                      <  2:553188/251‑1 (MQ=255)                                                                                 tAAAAACAATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACATGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGTGCTTTTTACAGGCTCTGGGCCAGCAGGGAAATGACAAAAGCCCCACTTATAAGCTGGTCGAACCCTATAAGCTAGACAAATTAATGGTTTATCACTTTGAtatg                                                                                                                                                                 >  2:8132/1‑248 (MQ=255)                                                                                   aaaaCCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACAGTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGTCGATTTAGGCGCAGGTAAAACCCCCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATCCGCTCGACAACTTAATGGTCTATCTCTTTGATGTGTa                                                                                                                                                               <  2:547173/251‑1 (MQ=255)                                                                                    aaaCCATGATGAATCGAGTAATTCCGCTCCCTGATGCGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTAc                                                                                                                                                              >  2:498214/1‑251 (MQ=255)                                                                                             tgaATCGAGTAATTCCGCTCCCTGAAGAGCAGGCAACATTAGACCTGGGCGAGAGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATATGTATGGCGATTTAGGCGCCGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTATGGGCCATAAGGGACATGTCAAAAGCACCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCGAACACTTTGCTTTGTACCGCCTTGcc                                                                                                                                                     >  2:552642/1‑251 (MQ=255)                                                                                                           ccGCTCCCTGCTGACCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGACAGCCTGCGCTGGCGCAACCGTAATATATCTGTATGGCGAATTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGCCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCCCTTTGATTTGTACCGCCTTGCCGATCCCGAGGCGCt                                                                                                                                       >  2:393705/1‑251 (MQ=255)                                                                                                               tcaatgatgcGCATGCAACATTAGACCTGGGCAAGGGGGTAGCGCAAACCTGCGATGGTGCAAGCGTAATCTATCTGTATGGCGCTTTTGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTGTCCAGGCGCTGGGCCATCAGGGTAATATCAAAAGCCCCACTTATACCCTGGTCGAAGCCTATACGCTCGACAACTTACTGGACTATCACTTTGATTTGTACCGCCTTGCCGCTCCCGAGGAGCTgg                                                                                                                                     >  2:28411/5‑249 (MQ=255)                                                                                                               tCCCTGATGAGCAGGCAACATTAGACCTGGGAGAGCGGGTAGCGAACGACTGCGATGGCGCAACCGTAATATATATGTATGGCGCTTTAGGCGCAGGTAACACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTCTCACGTTGAATTGTACCGCCTTGTCGCTCCCGAGGAGCTgt                                                                                                                                     >  2:69793/1‑248 (MQ=255)                                                                                                                      tgaGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGGCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATgg                                                                                                                            >  2:198484/1‑251 (MQ=255)                                                                                                                                  aTTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGatt                                                                                                                <  1:507865/251‑1 (MQ=255)                                                                                                                                      gACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCGTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACCTAATGTTCTATCACTTTGATTGGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGATTAttt                                                                                                            <  2:289328/251‑1 (MQ=255)                                                                                                                                           tggCGAGCGGGGAGCGAAAGCCTGCTACTGCGCAACCTTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCGCCTTTAGCCGGTGTTTTTTACAGGCTCTGGGCCAGCAGGGGAATGTCAAAAGCCCCTCTTATACTGTGGTCTTACCCGATGCGCGCGACAACTTAATGGTTTATCACGTTGATGTGTACCGGCTGGCCGATCCCGAGGAGCTGGATTTGATGGTGAGCCTCTATTATTTTGcc                                                                                                        <  2:45466/249‑1 (MQ=255)                                                                                                                                            gccgaacGGGTAGCGAAAGCCTGCGATGGCGTCACCGTAATCTCTCTGTATGGCGATTTAGGCGCAGGAAACACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGCAATGTCAAAAGCCCCACTTATAAGCTGGTCGCACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCACGATTATTTTGCCa                                                                                                       >  2:346027/7‑250 (MQ=255)                                                                                                                                                aGCGGGAAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATTGGGATCCGCGATTATTTTGCCAACGAt                                                                                                  >  2:143514/1‑251 (MQ=255)                                                                                                                                                 gCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGGGCTGGCGTTTATGGGGATCCGCGATTATTTTGCCAACGATg                                                                                                 >  2:212959/1‑251 (MQ=255)                                                                                                                                                   gggTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACATGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGATTATTTTGCCAACGATGcc                                                                                               <  1:503227/251‑1 (MQ=255)                                                                                                                                                    ggTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGGTTATTTTGCCAACGATGcc                                                                                               >  1:403057/1‑250 (MQ=255)                                                                                                                                                       agctaaaGCCGGCGATGGCGCAACCGTAATCGATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAACCGGGGCTTTTTACATGCTTTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGCCGAACCCTATACGCTCGACAACTTAATGGTCTTGCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGATCTGGAGGTTATTGGGTTCTGCGATTATTTTGCCAACGATTCCATCt                                                                                           <  2:510982/247‑1 (MQ=255)                                                                                                                                                        gCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGATTATTTTGCCAACGATGCCATCTg                                                                                          <  1:185275/251‑1 (MQ=255)                                                                                                                                                          gAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGAGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGATTATTTTGCCAACGATGCCATCTGcc                                                                                        >  2:512011/1‑251 (MQ=255)                                                                                                                                                            aaGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGATTATTTTGCCAACGATGCCATCTGCCtg                                                                                      <  1:87666/251‑1 (MQ=255)                                                                                                                                                                   cGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGATTATTTTGCCAACGATGCCATCTGCCTGGTGGAGt                                                                               <  1:509068/251‑1 (MQ=255)                                                                                                                                                                               cGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGATTATTTTGCCAACGATGCCATCTGCCTGGTGGAGTGGCCACAACAAg                                                                   <  1:200183/251‑1 (MQ=255)                                                                                                                                                                                    tctatctGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGATTATTTTGCCAACGATGCCATCTGCCtggtggcgt                                                                               >  1:478675/1‑231 (MQ=255)                                                                                                                                                                                    tctatctGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGATTATTTTGCCAACGATGCCATCTGCCTGGTGGAGt                                                                               <  2:478675/234‑1 (MQ=255)                                                                                                                                                                                      tatatgaaTGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCAGATCCCGAGGAGCTGGAGATTATGGGGATCCGCGATTATTTTGCCAACCATGCCATCTGCCTGTTGGAGAGGCCACACCAAGGTACAgg                                                            >  2:138571/8‑251 (MQ=255)                                                                                                                                                                                           gtagggcgtgtgaGGCGCAGGTAAAACCTCCGTTAGCTGGGTCCTTTTTTAGGGTCTGGGCCATCAGGGTAGTGTCAAAAGCCCCACTTGTACGCGGGTGGATCCTCGGACGCGCGGCAGCTTAATGGTTTGTCAGCTTGAGTGGTAGTGCTTTGCCGATCCCGGGGAGCTGGTGTGTATTTGGATCCGCGATTATTTTGCGAAGAAGGCCATCTGCCTGGTGGAGTGGCCACAACAAGGTACAGGTGttc                                                       <  1:542531/239‑1 (MQ=255)                                                                                                                                                                                               ggCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACGTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGATTATTTTGCCAACGATGCCATCTGCCTGGTGGAGTGGCCACAACAAGGTACAGGTGttcttc                                                    <  2:325168/250‑1 (MQ=255)                                                                                                                                                                                                gCGATTTAGGCGCAGGTCAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGATTATTTTGCCAACGATGCAATCTGCCTGGTGGAGTGGCCACAACAAGGTACAGGTGTTCTTCCt                                                  >  2:27784/1‑251 (MQ=255)                                                                                                                                                                                                     ttAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCTATCTTGTTGTTCTGGATTTTATTGGGTTCCGCGATTATTTTGCCAACGATGCCATCTGCCTGGTGGAGTGGCCACAACAAGGTACAGGTGTTCTTCCTGAcc                                              <  1:346673/250‑1 (MQ=255)                                                                                                                                                                                                            cAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGATTATTTTGCCAACGATGCCATCTGCCTGGTGGAGTGGCCACAACAAGGTACAGGTGTTCTTCCTGACCCGGATGTc                                      <  1:498214/251‑1 (MQ=255)                                                                                                                                                                                                               gTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTCATGTCAAAAGCCCCACTTATACGCTGGCCGAACCCTATACGCTCGACCACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGCTCAAGAGGAGCTGGAGTTTATGGGGATCCGAGATTCTTTTGCCAAAGATGCAATCTGCCTGGTGGAGTGGCCACAACAAGGTACAGGTGTTCTTCCTGACCCGGATGTCGa                                    >  1:560714/1‑250 (MQ=255)                                                                                                                                                                                                                 aaaaCCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGATTATTTTGCCAACGATGCCATCTGCCTGGTGGAGTGGCCACAACAAGGTACAGGTGTTCTTCCTGACCCGGATGTCGaaa                                  <  1:245859/250‑1 (MQ=255)                                                                                                                                                                                                                  aaaCCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGATTATTTTGCCAACGATGCCATCTGCCTGGTGGAGTGGCCACAACAAGGTACAGGTGTTCTTCCTGACCCGGATGTCGAAATa                                <  1:225412/251‑1 (MQ=255)                                                                                                                                                                                                                     cctccTTTAGTCTGGTCTTTTTACAGGCTCGTGGCCATCATGGTAATGTCAAAAGCCCCACTGATACGCTGGGCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGATTATTTTGCCAACGATGCCATCTGCCTGGTGGAGTGGCCACAACAAGGTACAGGTGTTCTTCCTGACCCGGATGTCGAAATaccc                             <  1:193218/248‑3 (MQ=255)                                                                                                                                                                                                                     ccaccTTTAGCCGGTGCTTTTTACAGGCTCTGGGCCATCAGGGGAATGTCAAAATCCCCACTTATACGCGGGTCGAACCCTATACGCTCGACAACTTAATGGGCTATCACTTTTATTTGGACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGATTATTTTGCCAACGATGCCATCTGCCTGGTGGAGTGGCCACATCAAGGTACAGGTGTGCTTCCTGACCCGGATGTCGAAATacac                             <  2:247168/251‑1 (MQ=255)                                                                                                                                                                                                                        ccTTTAGCCGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGATTATTTTGCCAACGATGCCATCTGCCTGGTGGAGTGGCCACAACAAGGTACAGGTGTTCTTCCTGACCCGGATGTCGAAATACACAtt                          <  2:161910/251‑1 (MQ=255)                                                                                                                                                                                                                               ccGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGATTATTTTGCCAACGATGCCATCTGCCTGGTGGAGTGGCCACAACAAGGTACAGGTGTTCTTCCTGACCCGGATGTCGAAATACACATTGATTATc                   >  1:114457/1‑251 (MQ=255)                                                                                                                                                                                                                                cGGGGCTTTTTACAGGCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGATTATTTTGCCAACGATGCCATCTGCCTGGTGGAGTGGCCACAACAAGGTACAGGTGTTCTTCCTGACCCGGATGTCGAAATACACATTGATTATCa                  <  1:503503/251‑1 (MQ=255)                                                                                                                                                                                                                                            cAGGCTCTGGGCCATCAGGGCAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGATTATTTTGCCAACGATGCCATCTGCCTGGTGGAGTGGCCACAACAAGGTACAGGTGTTCTTCCTGACCCGGATGTCGAAATACACATTGATTATCAGGCACAAGGCCg      >  2:105911/1‑251 (MQ=255)                                                                                                                                                                                                                                            cAGGCTCTGAGCCATCAGTGTAATGTCAAACGCCCCACTTATCCGCTGGTCGACCCTTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGCTCCCGAGGAGCTGGAGTTTCTGGGAATCCGCGATTATTTTGCCAACGATGCCATCTGCCTGGTGGAGTGGCCACAACAAGGTACAGGTGTTCTTCCTGACCCGGATGTCGAACTACCCATTGATTATCCGGCACAAGGCCg      >  2:556335/1‑251 (MQ=255)                                                                                                                                                                                                                                              ggCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGATTATTTTGCCAACGATGCCATCTGCCTGGTGGAGTGGCCACAACAAGGTACAGGTGTTCTTCCTGACCCGGATGTCGAAATACACATTGATTATCAGGCACAAGGCCGTg    >  2:425350/1‑251 (MQ=255)                                                                                                                                                                                                                                               gCTCTGGGCCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGATTATTTTGCCAACGATGCCATCTGCCTGGTGGAGTGGCCACAACAAGGTACAGGTGTTCTTCCTGACCCGGATGTCGAAATACACATTGATTATCAGGCACAAGGCCGTg    >  1:295246/1‑250 (MQ=255)                                                                                                                                                                                                                                                  ctGGGCCATCAGGGTAATGTCAAAAGCCCCACTTAGACGCGGGTCGAACCCTATACTCTCGACAACTTAATGGTCTATCTCTTTGATTTGTACCGCCTTGCTGATCCCGAGTAGCTGGAGTTTATGGGGATCCGCGATTATTTTGCCAACGATGCCATCTGCCTGGTGGAGTGTCTGCAACAAGGTACAGGTGTTCTTCCTGACCCGGATGTCGAAATACACATTGATTATCAGGCACAAGGCCGTGAg  <  1:296551/249‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    GCGCGTTTTGGAACGCGCGGGATGCTGGCAACCGATCTCTTTTCCACGCTACAGCGTATTGTTAACCCGGAAGTGACTGATAAAAACCATGATGAATCGAGTAATTCCGCTCCCTGATGAGCAGGCAACATTAGACCTGGGCGAGCGGGTAGCGAAAGCCTGCGATGGCGCAACCGTAATCTATCTGTATGGCGATTTAGGCGCAGGTAAAACCACCTTTAGCCGGGGCTTTTTACAGGCTCTGGGTCATCAGGGTAATGTCAAAAGCCCCACTTATACGCTGGTCGAACCCTATACGCTCGACAACTTAATGGTCTATCACTTTGATTTGTACCGCCTTGCCGATCCCGAGGAGCTGGAGTTTATGGGGATCCGCGATTATTTTGCCAACGATGCCATCTGCCTGGTGGAGTGGCCACAACAAGGTACAGGTGTTCTTCCTGACCCGGATGTCGAAATACACATTGATTATCAGGCACAAGGCCGTGAG  >  NC_000913/4395497‑4395986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑