Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,871,153	T→G	N51T (AAC→ACC)	truD ←	tRNA pseudouridine(13) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,871,153	0	T	G	100.0%	147.1 / NA	42	N51T (AAC→ACC)	truD	tRNA pseudouridine(13) synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/21);  total (21/21)

AGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTGGC  >  NC_000913/2870907‑2871390                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              aGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGgtttt                                                                                                                                                                                                                                           >  1:47697/1‑251 (MQ=255)          gCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCt                                                                                                                                                                                                                                  <  1:34038/251‑1 (MQ=255)                    tCAGCGCGCCTAAACGCAGCTGGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATTTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAat                                                                                                                                                                                                                         <  1:73945/250‑1 (MQ=255)                              tAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCtt                                                                                                                                                                                                              >  1:569097/1‑251 (MQ=255)                                       ctggcgcTTGTGCCGCGCATACTCCAGCCCCTGGCAGCCTTCCATTTGAAAGGCGCTCAGATCGGGCATTCCCTTCCCCGGCACGCGAGCGCATAACCCCTGTCCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATAAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAAc                                                                                                                                                                                                                               <  1:545567/222‑1 (MQ=255)                                       cTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGAGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGCGGATTCTAAc                                                                                                                                                                                                                               >  2:545567/1‑225 (MQ=255)                                                gtgCCGCGCATACTCCAGCACCGGGCAGCCTTCCAGTTGAAAGGCGCTCAGACCGGGCATTCCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGc                                                                                                                                                                                            <  1:150611/251‑1 (MQ=255)                                                     gcccatccTCCAGCACCTGGCAGCCCTCCAGTTGAAAGGCGCTCAGAGCGGGCATTCCCTTCCCCGGCGCGCGAGCGAATAACACCTTTTCCTTAACAGCATGTTTGTCTTTTTGCCCAGCAAAGCTGACTTCACGGGCATGATTTTTCAGAAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCaaa                                                                                                                                                                                       <  1:314692/244‑1 (MQ=255)                                                             ccacgccccTGGCACCCTTCCAGTTGAAAGGCGCTCAGAGCGGGTATTCCTTTGCCCGCCGCGGGGGCGCATAACCACTGTTCCGTAACAGCATGTTTGCTTTTTTGCCCAGCGAAGCTGACTACACGGGAATGATTTTTGAGGATTTTCGCCTTTCCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTccac                                                                                                                                                                               <  2:178665/244‑1 (MQ=255)                                                                               cccagtagaagggcgcctagattgtgCATTGCCGTGCCCGGCACGCGTGCCCATACCCACTGTTCGGTAACAGAATGTTGTTTTTTTTCCCCAGCGAAGCTGACTTCAGGGTCATGAATTTTCAGTATTTTCACCAGTGCAGCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCg                                                                                                                                                              <  2:497480/225‑1 (MQ=255)                                                                                ccAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTCTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCgg                                                                                                                                                             >  2:300856/1‑250 (MQ=255)                                                                                             gcTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTcagc                                                                                                                                                >  1:108206/1‑250 (MQ=255)                                                                                                     cGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCgggg                                                                                                                                       >  2:493166/1‑249 (MQ=255)                                                                                                                  gCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTAc                                                                                                                          >  1:431205/1‑251 (MQ=255)                                                                                                                        cACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGCATTTCGCCAGTGCATCAGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCACATCTTCCACCACAACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCgagg                                                                                                                     >  2:178080/1‑247 (MQ=255)                                                                                                                              aGCGCATAACCGCTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTATAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTa                                                                                                                >  2:427982/1‑249 (MQ=255)                                                                                                                               gcgcATAACCACTTTCCCGTAAAAGCATGTTTGTTTTTTTCCCCAGCGAAGCTGACTTCACGGGCATAATTTTTCAGAAATTTCCCCAGTGAATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGt                                                                                                             <  2:108206/251‑1 (MQ=255)                                                                                                                                cgcATAACCACGGTTCCGTAACAGCATGTTTGTCTTTTGGCCCAGCGAAGCTGACTTCACGGGCATGATTTTTCGGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCGTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTg                                                                                                            <  2:304084/251‑1 (MQ=255)                                                                                                                                            gTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAAc                                                                                                >  1:12005/1‑251 (MQ=255)                                                                                                                                                 gTAAAAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATAAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAAt                                                                                           <  1:580127/251‑1 (MQ=255)                                                                                                                                                                     ttGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCGCCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATg                                                                         >  1:427524/1‑249 (MQ=255)                                                                                                                                                                      tGCCCAGCGAAGCTGAATTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCACACTCAATCATTTTGTTGCCTTAATg                                                                         >  2:73913/1‑248 (MQ=255)                                                                                                                                                                       gcccggctactcctacttccggggCATGAATGTTTAGTACTTTCGCCTGTGCCACCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTa                                                                     <  2:440502/230‑1 (MQ=255)                                                                                                                                                                                   tGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGATTTGGCTTTCAGCAGCCCGGAGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCCACCTCAATCATTTTGTTGCCTTAATGAGTAGCGCCAACGCtt                                                         >  2:461199/1‑251 (MQ=255)                                                                                                                                                                                    gacatcccGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCt                                                          <  1:102778/242‑1 (MQ=255)                                                                                                                                                                                      cTTCACGGGCATGACTTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGATTcac                                                      >  1:399733/1‑251 (MQ=255)                                                                                                                                                                                          aCGGGCATGAATTTTCAGAAATTTCGCCAGTGCTTCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGc                                                  <  1:493166/251‑1 (MQ=255)                                                                                                                                                                                                            gAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCgg                                                                                                                               >  1:125251/1‑156 (MQ=255)                                                                                                                                                                                                            gAATTCCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCgg                                                                                                                               <  2:125251/156‑1 (MQ=255)                                                                                                                                                                                                              aTTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCgg                              >  1:332036/1‑251 (MQ=255)                                                                                                                                                                                                                ttcgcatgTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCGAAGCCCAAATCTTCCACCCCCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGt                             <  1:503455/243‑1 (MQ=255)                                                                                                                                                                                                                        aacATCCGCCACAAAACGGGTATTGCAGCCGGTGTTGAGGATTCTAACTAGAATATGCTCACCTTCGCCATCAGGCTCAAAGCCCAAATCGTCCACCACCCCAAAGTCGTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTTCCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTGGACTTAATGAGGAGCGCTAGCGCTTACCAGGCAATCCCTTCCCCACGGCCGGTAAATCCCAg                    <  2:408447/249‑1 (MQ=255)                                                                                                                                                                                                                          cATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGtt                  <  1:557805/251‑1 (MQ=255)                                                                                                                                                                                                                          cATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGtt                  >  1:511206/1‑251 (MQ=255)                                                                                                                                                                                                                              cgccgcAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCTCCTCCACAAAGTCTACCTGATTGGCTTTCAGCAGCCCGGTGCCTTGCTGTTTACCGTGGAGGTAAGTGAGATTATCCAACTCAATCATTTTGTGGCCTTAATGAGAAGCGCCTCCGCTTCACAGGCAATCCCTTCCCCACGGCCGGTAAATCCCAGtgttgc              <  2:170808/251‑6 (MQ=255)                                                                                                                                                                                                                               gCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTcc             >  1:477522/1‑251 (MQ=255)                                                                                                                                                                                                                                 cacaAAACGGGTATTGCAGCCTGTTTTGAGGACTCTAACCAGAATATGCTCACCTTCACCATCAGTATCAAAGCCCAAATCTTCCCCCACCGCAAAGTCTTGCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTTATATTATCAAACGCAATCATGTTGTTGCCCTAATGATTAGCGCCACCGCTTCACAGGCAAGCCCTTCCCCACGTCCGGTAAATCCCAGTTTTACCga           <  2:28040/251‑2 (MQ=255)                                                                                                                                                                                                                                     aaaCGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGCTTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCgtagt        >  1:101920/1‑250 (MQ=255)                                                                                                                                                                                                                                        cGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGGTTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTg    >  1:148298/1‑251 (MQ=255)                                                                                                                                                                                                                                         gggTATTGCAACCGGTTTTGAGGCTTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTTCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCACACTCAATCATTTTGTTGCCTTAATGATTAGCGCCCCCGCTTAACAGGCAATCCCTTCCCCACGTCCGGTCAATCCCAGTTTTTCCGTAGTAGTgg   >  2:237343/1‑251 (MQ=255)                                                                                                                                                                                                                                          ggtcttGCAGCCGGTGTTGAGGATTCTAACCAGAATCTGCGCCACTTCAACATCAGGACCAAAGCCGAACTCTTCCACCACCCCCAAGTCGTCCGGATTGGCTTTCATCAGCCCGGCGCCTTGCGGTTTACCGTGGAGGTAAGGGACATTCTCAAACTCGATCATTTTGTTGCCGTAATGAGTCGCGCCACGGGTTCATAGGCAATCCCTTCCCCCCGTACGGTAAATCCCAGTTTTTCCGTAGTAGTGGc  <  1:291545/247‑1 (MQ=255)                                                                                                                                                                                                                                                  aGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCAttt                                                                                      <  1:493047/159‑1 (MQ=255)                                                                                                                                                                                                                                                  aGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCAttt                                                                                      >  2:493047/1‑159 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              AGGGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTGGC  >  NC_000913/2870907‑2871390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑