Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,814,461	C→T	L91L (CTG→CTA)	luxS ←	S‑ribosylhomocysteine lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,814,461	0	C	T	100.0%	145.9 / NA	41	L91L (CTG→CTA)	luxS	S‑ribosylhomocysteine lyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/24);  total (17/24)

GTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCAATCAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGT  >  NC_000913/2814224‑2814700                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                gtgCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATaaaa                                                                                                                                                                                                                                   <  1:201813/251‑1 (MQ=255)  tgCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATaaaa                                                                                                                                                                                                                                   >  2:273504/1‑250 (MQ=255)    cAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATCCTGCGAGCAATATCCTGCGCTTCCTGCAACCAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCAAGGCATCAGCAACACGCTGCTCATCTGGCGAACCA‑TTAGCCTCATATAAAAAcc                                                                                                                                                                                                                                >  1:580690/1‑251 (MQ=255)       ttCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGt                                                                                                                                                                                                                             >  1:585643/1‑251 (MQ=255)             cAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCCGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGc                                                                                                                                                                                                                        >  2:279876/1‑250 (MQ=255)             cAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTGCCAGGCATCAGCAACGCGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCa                                                                                                                                                                                                                       <  2:196666/251‑1 (MQ=255)               aCTTCTCGTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGATTGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGc                                                                                                                                                                                                                     <  2:448337/251‑1 (MQ=255)                  tctcTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGccc                                                                                                                                                                                                                   <  2:206149/250‑1 (MQ=255)                     ctTTCGGAAGTACCAGTTCTTCGATGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCACGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAAGCGGTGCGGCAGCCCATTg                                                                                                                                                                                                               >  2:308839/1‑251 (MQ=255)                         cGGCCGTGCCAGTTCTTCGTTGCGGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCg                                                                                                                                                                                                            <  2:285506/250‑1 (MQ=255)                             aGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGat                                                                                                                                                                                                        >  2:313166/1‑250 (MQ=255)                                  cAGTTCTTCGTTGCTGATGATGCGTACGTCACTTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTGTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGAt                                                                                                                                                                                                  <  1:471725/251‑1 (MQ=255)                                        ttcGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAAtctc                                                                                                                                                                                            >  1:335277/1‑251 (MQ=255)                                           gTTGCTGTGGATGCGTACGTCACGGTCCAGAATGCTACTCGCAATATCCTGCGCTTCCTTCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACTCGCTGCGCATCTGGCGTACCG‑TTAGACTCATAGAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCTATAATCTCTac                                                                                                                                                                                         <  2:227344/251‑1 (MQ=255)                                                    gATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTAcc                                                                                                                                                                                >  1:238359/1‑251 (MQ=255)                                                        cGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGtt                                                                                                                                                                             >  1:37372/1‑250 (MQ=255)                                                           ccggcACGTTCCAGAATGCTACGCGCAATATCCGGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTTCACCATTACCGTTAAg                                                                                                                                                                          <  2:53315/246‑1 (MQ=255)                                                                    tCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACg                                                                                                                                                                 <  1:200410/250‑1 (MQ=255)                                                                       aGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATa                                                                                                                                                             <  1:303181/251‑1 (MQ=255)                                                                         aaTGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATaaa                                                                                                                                                           <  1:151059/251‑1 (MQ=255)                                                                                cgcgCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCa                                                                                                                                                    <  2:429569/251‑1 (MQ=255)                                                                                        atCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTATACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGAGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATACAACCAGCAAACAGGt                                                                                                                                             <  2:457352/250‑1 (MQ=255)                                                                                          ccTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCTACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCt                                                                                                                                          <  2:447573/251‑1 (MQ=255)                                                                                            tGCGCTTCCTGAAAAGAGTGCACCTCATAAGTGCCACACCGGTCGACGTTCAGTTCCGGGATCTGATTCTGCTCCTGCCCTTTCAGCACGCCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGCGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACACGCAAACATGTACTcc                                                                                                                                        >  2:538480/1‑251 (MQ=255)                                                                                             gcgcTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCa                                                                                                                                       <  1:152152/251‑1 (MQ=255)                                                                                                cTTCCTGCAACGAGTGCACCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCCGCACGTCTTCCATTGCCGCTTTACAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAACCCAGCAAACAGGTGCTCCAggg                                                                                                                                    >  1:222826/1‑251 (MQ=255)                                                                                                             gTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCctct                                                                                                                       <  2:585643/251‑1 (MQ=255)                                                                                                                  tCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGCTTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTg                                                                                                                  >  2:371432/1‑251 (MQ=255)                                                                                                                     ggTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGc                                                                                                                >  2:144791/1‑250 (MQ=255)                                                                                                                            gCCACACTGGTAGACGTTCAGTTCCGGGAACTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCCGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGAAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCATCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCActtc                                                                                                        >  2:352650/1‑251 (MQ=255)                                                                                                                                 acTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATCTAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGt                                                                                                   >  1:582353/1‑251 (MQ=255)                                                                                                                                    ggTAGACGTTCAGTTCCGGTATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCAGCAGCAACTCGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAACCCGGTGCGGCAGCCCATTTGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCTTAAAACCAGCAAACAGGTGCTCCAGGGTATGTATCCCTCTTTCTGGCATCACTTCTTTGTTc                                                                                                 <  2:525927/250‑1 (MQ=255)                                                                                                                                              cAGTTCCGGGATCTGATTCTGATCCTGCACGTTCAGCACGTCTGCCATTGCCGCTTTCCAGGAATCAGCAACACGCTGCTCATCTGGCGGACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCGCTACTCCATTACCGTTAAGATGGTTACGCAGAAAACCAGCAAACAGGTGCTCCAGGGTAAGGATCCCTCTATCTGGCATCACGTCTGTGTTCGGAACGCAGaa                                                                                      <  2:56843/251‑1 (MQ=255)                                                                                                                                                                                                  gCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTc                                  <  1:391049/251‑1 (MQ=255)                                                                                                                                                                                                    tttCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACATCTTTGTTCGACACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGc                                >  2:4993/1‑251 (MQ=255)                                                                                                                                                                                                     ttCCAGACATCAGCAACACGCTGCTCATCTGGCGAACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCCTGAGGGGTGTTCATTGTTTTCGcc                               >  2:152542/1‑251 (MQ=255)                                                                                                                                                                                                      tCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGcc                               <  1:91613/250‑1 (MQ=255)                                                                                                                                                                                                       ccAGGCATCAGCAACACGCTGCTCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGGATGGATCCCTCTTTCTGGCATCACTTCGTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTTTTCATTGTTTTCGCCAc                             <  2:272352/251‑1 (MQ=255)                                                                                                                                                                                                                         cggatcagcTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCGCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTTTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTc           <  1:214095/243‑1 (MQ=255)                                                                                                                                                                                                                           gctCATCTGGCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCa         <  1:273504/251‑1 (MQ=255)                                                                                                                                                                                                                                   ggCGTACCA‑TTAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGt  <  1:79760/250‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                GTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCACACTGGTAGACGTTCAGTTCCGGGATCTGATTCTGATCCTGCACTTTCAGCACGTCTTCCATTGCCGCTTTCCAGGCATCAGCAACACGCTGCTCATCTGGCGTACCAATCAGACTCATATAAAAACCGGTGCGGCAGCCCATTGGCGAGATATCGATAATCTCTACACCATTACCGTTAAGATGGTTACGCATAAAACCAGCAAACAGGTGCTCCAGGGTATGGATCCCTCTTTCTGGCATCACTTCTTTGTTCGGCACGCAGAAGCGCAGATCGAACACGGTGATTGCGTCGCCATGCGGGGTGTTCATTGTTTTCGCCACCCGAACTGCAGGCGCTTCCATCCGGGT  >  NC_000913/2814224‑2814700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑