Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,523,109	G→A	L293L (CTG→TTG)	xapB ←	xanthosine:H(+) symporter XapB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,523,109	0	G	A	100.0%	91.5 / NA	27	L293L (CTG→TTG)	xapB	xanthosine:H(+) symporter XapB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/15);  total (12/15)

CCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAG‑CAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCAAAGAGGAAAAA  >  NC_000913/2522884‑2523349                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 ccatgccaCTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGCAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAG‑CAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGc                                                                                                                                                                                                                         >  2:313392/1‑251 (MQ=255)      ccaCTCAGAAGCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAG‑CAAAAGAAATCCGGTTGGTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGAc                                                                                                                                                                                                                    <  1:65334/251‑1 (MQ=255)              aaTCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGCGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGACATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAG‑CAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTa                                                                                                                                                                                                             >  1:370938/1‑250 (MQ=255)                            aTGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAG‑CAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCttt                                                                                                                                                                                              <  1:313392/251‑1 (MQ=255)                               cgcCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAG‑CAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACGCATCAGCATTACGGTTTTAATGCCAAATCGCTTTaaa                                                                                                                                                                                           <  2:357366/251‑1 (MQ=255)                                    acacCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAG‑CAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGaa                                                                                                                                                                                      <  1:225703/251‑1 (MQ=255)                                             aCCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAG‑CAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGt                                                                                                                                                                             <  1:450923/251‑1 (MQ=255)                                                     cATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAG‑CAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATaa                                                                                                                                                                     <  1:481994/251‑1 (MQ=255)                                                        aaaGAGCCCCTTCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACCCCCTTAAACAATCCTCGTCAGCAGCAG‑CAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGc                                                                                                                                                                  >  2:430156/1‑251 (MQ=255)                                                        aaaGAGCCCATGCGCGCTGGCACGAATGCTGGAAGCAACTTCCGTTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAG‑CAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCAACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATaaaa                                                                                                                                                                   <  2:2430/250‑2 (MQ=255)                                                           gagCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAG‑CAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCa                                                                                                                                                               <  1:524494/251‑1 (MQ=255)                                                                     gcgcTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAG‑CAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCAt                                                                                                                                                     <  1:508527/251‑1 (MQ=255)                                                                              cGAATGCTGGAATCAACTGCCTGTTCGACAAATACCGAACCAGAAATATGGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAG‑CAAAATAAATCCGGTTGTTGACGGATCGCCATATGCGAATAAGCCAAAGCGCAACGTCCAGGCCACCAGACTCATCAGCATGACGGTTTTAATGCCAAATCGCTGTAAAAAGAATGGGAGAGTCAGTATAAAGCCCACTTCTGTCATCTGTGAAAc                                                                                                                                            <  2:54955/251‑1 (MQ=255)                                                                                aaTGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAG‑CAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTg                                                                                                                                          >  1:591481/1‑251 (MQ=255)                                                                                      ggAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAG‑CAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGt                                                                                                                                     <  1:181257/250‑1 (MQ=255)                                                                                                tCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAG‑CAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAg                                                                                                                            <  1:172710/249‑1 (MQ=255)                                                                                                        gACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAG‑CAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGACAAATCGCTTTAAAAAGAATGGGATAGGCAGTATAAAGCCCACTTCTGCCATCTGAGAAACTGACAGTAAAATGGAGGGCTATTTcc                                                                                                                  >  2:235716/1‑250 (MQ=255)                                                                                                                            tatTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAG‑CAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAAc                                                                                              >  1:23459/1‑251 (MQ=255)                                                                                                                                  agaaATCGAATACAGAGCCATAAACAATCATCGACAGCGGCAG‑CATAAGAAGTCCGGTTGTTGAGGGATGAGCATAGGAGAAGAAGCCAGAGGGGAACGTCCAGGCCACCATACTCGTCAGGATGACGATATTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCggg                                                                                        <  1:430156/251‑1 (MQ=255)                                                                                                                                      aTCGACTGCACAGCCATAAACAATCATAGCCAGCAGCTG‑CAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTAATCAGCATGACGGTTTTAATGCAAAATCGGTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGAGAAACTGGCAGTACAATGGAGGGATATTTTACCACAAAACTGTCAGCAAACTACGGGTTAc                                                                                    >  2:392565/1‑251 (MQ=255)                                                                                                                                                       aaaCAATCATCGACATCAGCAG‑CAAAATAAATCCGGGTGTTGACGGATCGACATAGGCGAAGAAGCCAAAGCGCAACGTCCATGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCAGCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGTGTTTCGGGCGAAATCATGTAgg                                                                   <  2:109094/251‑1 (MQ=255)                                                                                                                                                               atcGACAGCCGAAGACAAAA‑AAATCAGGTTGGTGACGGCTCGCCATAGGCGAAGAAGCCAAAACGCAACGTCCAGGCCCCCATACTCATCCACATGACGGTATTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCCTCTGTGAAACTGACAGTAAAATGGCGGGATATTTCACCACAAAACTGTCAGCAAACTCAGGGATACGGGCGCAATCATGTAGGAACGGAtt                                                           >  2:92731/1‑251 (MQ=255)                                                                                                                                                                 cGACAGCAGCAG‑CAAAATAAATCCGGTTGTTGACGGATCGCCATAAGCGAAGAAGCCAAAGCGCAACGGCCAGGCCACCATACTCATCAGCCTGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTACAATGGAGGGATATTTCACCACAAAACTGCCAGCAAACTCCGGGTTAAGGGAGAAATCCTGTAGGAACGGATTAc                                                         >  2:169241/1‑251 (MQ=255)                                                                                                                                                                                aaataaatCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGt                                            >  1:188769/1‑250 (MQ=255)                                                                                                                                                                                          ggTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGt                                  >  1:424894/1‑250 (MQ=255)                                                                                                                                                                                                                         ccAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCAAAGAGGaaaaa  <  1:340188/251‑1 (MQ=255)                                                                                                                                                                                                                            aaGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCAAAGAGGaaaa   >  1:276435/1‑247 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 CCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAG‑CAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCAAAGAGGAAAAA  >  NC_000913/2522884‑2523349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑