Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,484,827 C→T L152F (CTT→TTT)  evgS → sensor histidine kinase EvgS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,484,8270CT95.5% 60.9 / ‑4.5 23L152F (CTT→TTT) evgSsensor histidine kinase EvgS
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (7/14);  minor base G (0/1);  total (7/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.49e-01

GTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCT‑TCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACT  >  NC_000913/2484583‑2485052
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                  
gtttctcatcGATTTGCAGAAACGTGTTCGTTGTCTTAATGCTGATTATTTAAATCTTTTTAAAATAGCGTTAAATATCACATTAACAATTCGGGAATACGCTGATCATCAAAAAGCAATGGACGCGCTTGTAGAAGGTGAAGTTGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATACTGACATTGCTGCAACCATATCATCGATAAGTACTGCTCCGGCGCAGGTATCGACCTT‑TCAc                                                                                                                                                                                                                              <  2:579334/241‑1 (MQ=255)
                  gcaACGGGTTCGTGATATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTTCCTTTCCGGCGCTGGTAACCACCTT‑TCACGACTCAATGCGACCGCTTa                                                                                                                                                                                                           <  2:231075/251‑1 (MQ=255)
                      cGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTCAATATCAAATTAACCCTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGACGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTCCCTTTCCGGCGCTGGTAACCACCTT‑TCACGCCTCAATGCGACCGCTTCCCTc                                                                                                                                                                                                       >  2:250020/1‑251 (MQ=255)
                                           gATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAGGGCGAAGTCGATATAGTGTTATCACATTTAGTTTCTTCGCTGCCTCTTAATAATGCGAGTGCGGCAACCAAACCATGGATAATTTCCTATACGGCGCTGGTAACGAGCGT‑TCGCGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTg                                                                                                                                                                                   <  1:274543/250‑1 (MQ=255)
                                                 ttAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTT‑TCACGACTCAATGCGACCGCTTACATCACCAAAACAAGTAAATATTGCTCGGGt                                                                                                                                                                            >  2:489567/1‑251 (MQ=255)
                                                      tCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGAAGCTCATCAAAAAGCAATGGAAGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATATTTACCTTTCCGGCGCTGGTAACCACCTT‑TCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGATCGGGTAGcc                                                                                                                                                                        >  2:536160/1‑249 (MQ=255)
                                                      tCTTTTAAAAAGAGCGTTAAAAATCAAATTAAAACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTACAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGATGCAAACAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTT‑TCACGACTCAATGCTTCAGCTTACCACACCAAAACTAGTAAATATTGCTCGGGTAGCa                                                                                                                                                                        >  2:316381/1‑250 (MQ=255)
                                                            aaaaaGAGCGGTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGTACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTTCTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTGTCCGGCGCTGGTAACC‑CCCTGTCTCGACTCAATGCGACCTCTTTCCTCACCAAAACCAGTGAATATTGCTCGTTTAGCAAATTAcc                                                                                                                                                                 <  2:573086/251‑1 (MQ=255)
                                                              aaaGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTGCCGGCGCTGGTAACCACCTT‑TCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcccc                                                                                                                                                               <  1:536160/251‑1 (MQ=255)
                                                              aaaGAGCGGTAAACATCAAATTAACACTCAAGGAATCCTCAGATCATCAAAAAGCAATGGAAGCGCTTGCAGAAGGTGAAGACGATATAGTGTTATCACATTTAGTCTCGTCCCCGCCTCTTAATAATGATATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCTCTGGGAACCACCTT‑TCTCGACTCAATGCTACCGCTTACCTCACCAAAACCAGTAATTATTGCTCGCGTAGCAAAtttcacc                                                                                                                                                               <  2:439421/251‑6 (MQ=255)
                                                                        aaaTATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATCGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTT‑TCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAg                                                                                                                                                      <  1:402662/250‑1 (MQ=255)
                                                                            atCAAATTAACACTCCGGGAATACGCAGAGCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTT‑TCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGATGTAAt                                                                                                                                                 <  2:551847/251‑1 (MQ=255)
                                                                                aaTTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTAATTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTT‑TCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCATACGAGGTAATtcat                                                                                                                                             <  2:97389/251‑1 (MQ=255)
                                                                                 aTTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTT‑TCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATtcatc                                                                                                                                            >  1:302031/1‑251 (MQ=255)
                                                                                                       gATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTT‑TCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGATGTAATTCATCAATCATTTCCAAAAGCAACAAt                                                                                                                      <  1:489567/251‑1 (MQ=255)
                                                                                                       gATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTT‑TCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAAt                                                                                                                      >  1:580566/1‑251 (MQ=255)
                                                                                                                  aGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTT‑TCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                                                           <  1:66197/251‑1 (MQ=255)
                                                                                                                          aCGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTT‑TCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTta                                                                                                   <  1:8430/251‑1 (MQ=255)
                                                                                                                                                                                                 aTTGCTGCAACCAAGCCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTT‑TCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTGCAAATTTATATCAGGCATTAGCATCCGGCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACcagcag                            <  1:35140/251‑1 (MQ=255)
                                                                                                                                                                                                        cAACCAAACCAATGATAATTTCCTTTCCGGCGCTGGTAACCACCTG‑TCACGACTCAATGCGACCGCCTACCGCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGAtt                     <  2:580566/251‑1 (MQ=255)
                                                                                                                                                                                                              aaccaTTGATAATTACCTTTCCGGCGCTGGTAACCACCTT‑TCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGc               >  1:128705/1‑251 (MQ=255)
                                                                                                                                                                                                                        aaTTACCTTTCCGGCGCTGGTAACCACCTT‑TCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATGTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTtcactc     <  1:343885/251‑1 (MQ=255)
                                                                                                                                                                                                                            cccTTTCCGGCGCTGGTAACAATCTT‑TCACGAATCAATGCGACCGCTTACCTCAACAAAACCAGTAAATAGTGCTCGGGTAGCAAATTACCCCACAGATGAGGTAATTCATCAATCATTTCCAAAAGCAACAAGTAACGCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTAGTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTcactcact  <  2:181799/249‑1 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                  
GTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCT‑TCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACT  >  NC_000913/2484583‑2485052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑