Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,301,998	A→G	S273G (AGC→GGC)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,301,998	0	A	G	100.0%	171.6 / NA	53	S273G (AGC→GGC)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/22);  total (31/22)

TCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACT  >  NC_000913/1301752‑1302243                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      tCACCTGTGCCAAAAGCCGCTATCGAGAACTTCGGCGTAAAATGTACCCAGCCTGGTAATATCGTAACCAAAGGGGCCTATACATTAACAGATTGGGTCGTAAAAGAACGAATCGTTATTGAACGCAGCCCGAACTACTGGAACAACGCGACAACCGTTATTAACAAGGTAACCTATTTGCATATTGCTTCTGAAGTTACCGATGTCAACCGCTACAGTAGTGGTGCAATCGACATGACGAATAACGGCAt                                                                                                                                                                                                                                                   >  2:595154/1‑251 (MQ=255)  cgccGGTGCCAAAAGCCGCTATCGAGCAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCTAAATCCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCTTAGTGGTGAAATCGACATGACGAATAACGGCAt                                                                                                                                                                                                                                                   <  2:311152/248‑1 (MQ=255)        tGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATc                                                                                                                                                                                                                                            >  1:172157/1‑251 (MQ=255)        ggCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATAGCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATc                                                                                                                                                                                                                                            <  2:184030/250‑1 (MQ=255)              aaGCCGCTATCGAGAAATTCGGCAAAACATGGACCCAGCCTGGTAATATCGTCACCAACGGCGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGACGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGCTCGAATTg                                                                                                                                                                                                                                      >  2:535635/1‑251 (MQ=255)               aGCCGCTATCGAGAAATTCGGCTAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCGACGGGAACAACGCGAAAACCTTTATTAACCAGGTAGTCTATTTGCCTATTGCTTCTGAAGTGACCGATGTCAACCGCGACCGTAGTGGTGAAAGCGACATGACGAATAACGTGATGCCGATCGTATTGt                                                                                                                                                                                                                                     <  2:577166/251‑1 (MQ=255)                                        aaaTGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCGTGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATccc                                                                                                                                                                                                            >  2:146713/1‑251 (MQ=255)                                         aaTGGACCCAGCCTGGTAATATCGTCACCAAAGGTGCCTCTACCTTACAAGATTGACGCGTAACCGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGATATTAACCAGGAAACCTATTTGCCTATAGCTTCTGAAGTTACCGATGTCAACCGCTCCCGTAGTGGTGAAATCATCATGACGAATACCGGCATGCCGATCGCATTGTTCCAGAAGCTGAAAAAAGAGATCCCg                                                                                                                                                                                                           >  2:504705/1‑251 (MQ=255)                                           tGGACCCAGCCTGGTAATATCGTCACCAACGGTTCATATACCTTAAAAGATTGGGTAGTAAACGAACGAATCGGTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGCAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTCGTAGTGAAATCGACATGACGAATAACGGCCTGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCgg                                                                                                                                                                                                          >  2:137003/1‑250 (MQ=255)                                                           atatCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTTAAAAAAGAGATCCCGGACGAAGTTCACGTTGAt                                                                                                                                                                                         <  1:481610/251‑1 (MQ=255)                                                            tatCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCGCGGACGAAGGTCACGTTGATc                                                                                                                                                                                        >  2:314054/1‑251 (MQ=255)                                                                    ccAACGGTGCCTATACCGTAAAAGAGTTGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCCGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGTACGAAGTTCACGTTGATACATACCtg                                                                                                                                                                                <  2:555594/251‑1 (MQ=255)                                                                        cGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGc                                                                                                                                                                             >  2:12349/1‑250 (MQ=255)                                                                           tGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGCTCGAATTGTTCCAGAAGCTGAAAACAGAGATCCCGGACGAAGTTAACGTTGATCCATACCTGGGCACtt                                                                                                                                                                         >  2:189510/1‑251 (MQ=255)                                                                                      tAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACg                                                                                                                                                                <  2:342961/249‑1 (MQ=255)                                                                                         aagattttgTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGACCAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGCTGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAACAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTActatatt                                                                                                                                                           >  2:60428/9‑245 (MQ=255)                                                                                          agagtGGGTCTTAAACGAACGAATCGTTCTTGAACGCAACCCGACCGACTGGAGCAACGCGGAAACCGTTATTAACCAGGTAACCTATTTGCCTAGTGCTTCTAAATGTAGCGATGTCAGCCGCTACCGGATGGGGGAAATCGACATGACGAATAACGGCAGGCCGAGCGAATTGTGCCAGAAGCTTAAAAAAGAGATCGCAGACTATGTTCACGTTGGTCCTTACGTGTGCAcagagtattgcggattta                                                                                                                                                          <  2:356039/247‑18 (MQ=255)                                                                                          aGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTa                                                                                                                                                          <  1:12349/251‑1 (MQ=255)                                                                                           gATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTa                                                                                                                                                          >  1:92836/1‑250 (MQ=255)                                                                                                 gTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCGATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTGCCAGAAGCTGAAAAAAGAGATCCCGGACTAAGTGCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCa                                                                                                                                                   <  1:307895/251‑1 (MQ=255)                                                                                                   cGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAg                                                                                                                                                  <  1:170920/250‑1 (MQ=255)                                                                                                          gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGcc                                                                                                                                          >  1:57988/1‑251 (MQ=255)                                                                                                                   gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACg                                                                                                                                  >  2:93530/1‑250 (MQ=255)                                                                                                                                                caacGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGCATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGCAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATCTGCGTGTGCGTACCGCACTGAAACt                                                                                                      >  1:354034/1‑249 (MQ=255)                                                                                                                                                caacGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAg                                                                                                    <  1:152392/251‑1 (MQ=255)                                                                                                                                                 aacGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCGGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCGTGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAgg                                                                                                   <  1:146713/251‑1 (MQ=255)                                                                                                                                                        aaaCCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGAc                                                                                            >  1:501550/1‑251 (MQ=255)                                                                                                                                                            cGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACcgcg                                                                                        <  1:382057/251‑1 (MQ=255)                                                                                                                                                            cGTTATTAACCACGTAACCTATTTGCCTATTGCTTCTGACGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGTCATGACGAATAAAGGAATGCCGATCGAATTGTTCAAGACGCTGAAAAAAGAGATCCCGGAAGAAGTTCACGTTGATCAATACCTGTGCACTTACAATTAAGAAATTAACAAACAGAAACCGCCATTCAACGCTGTGCGTGTGCGTACAGCACTGAACATAGGTATggccagcg                                                                                        >  2:289840/1‑245 (MQ=255)                                                                                                                                                             gTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACcgcg                                                                                        >  1:448968/1‑250 (MQ=255)                                                                                                                                                               tattaACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACcgcg                                                                                        <  1:128911/248‑1 (MQ=255)                                                                                                                                                               tattaACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACcgcg                                                                                        >  2:128911/1‑248 (MQ=255)                                                                                                                                                                attaACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGatat                                                                                    >  1:570449/1‑251 (MQ=255)                                                                                                                                                                     ccAGGTAACCTATTTGCCTATTGCTTCTGAAGTTTCCGATGTCAACCGCTACCGTAGTGGTGAAAGCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGTACGAATTTCACGTGGATCCATACCTGTGCACGTACTATTCCTAAATGAACAACCATAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTg                                                                               <  1:595154/251‑1 (MQ=255)                                                                                                                                                                       aGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGtt                                                                             <  1:128273/251‑1 (MQ=255)                                                                                                                                                                         gTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTta                                                                            >  1:106292/1‑250 (MQ=255)                                                                                                                                                                         gTAACCTATTTGCCTATTGCTTATGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTta                                                                            >  1:187052/1‑250 (MQ=255)                                                                                                                                                                           aaCCTCTTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGACATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGCtaat                                                                          >  2:544332/1‑250 (MQ=255)                                                                                                                                                                             catatttgctttttgcgccTGAAGTGACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATaaa                                                                       <  2:333991/233‑1 (MQ=255)                                                                                                                                                                                  ttGCCTATTGCTTCTGAAGTTCCCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGCTCGACTTGTTCCAGAAGCTGACAAAAGAGATCCCGGCCGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCCGAACCCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTCGGAATGGACCGCGATATCATTGTTAATAAAGTGa                                                                   >  2:195256/1‑250 (MQ=255)                                                                                                                                                                                  ttGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGa                                                                   >  1:328945/1‑250 (MQ=255)                                                                                                                                                                                  ttGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGGGAAATAGACAGGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCGGAAAAAAGAGATCCCGGACGAAGTTAACGTTGATCCATACCGGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGa                                                                   <  1:439736/250‑1 (MQ=255)                                                                                                                                                                                     ccTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAACTCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAgc                                                               >  1:46389/1‑251 (MQ=255)                                                                                                                                                                                        aTTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATTGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGACAgcg                                                              <  2:320935/249‑1 (MQ=255)                                                                                                                                                                                        aTTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACGACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGAGAAAgcgc                                                             >  1:354268/1‑250 (MQ=255)                                                                                                                                                                                                      tACCGATGTCAACAGCTACCGTAGTGGTGAAATCGAAATGACGAATAACGGCATGCCGATCGAATTGTTACAGAAGCTGAAAAAAGAGGTCCCGGACGAAGTTCACGTTGATCCATACCTGAGCACTTACTATTACGAAATTAACAACCAGAAACAGCAATTCAACGATGTGCGTGTGCGTACAGCACTGAAACTAGGTATGGACCGCGATATCGTTGTTCATAAAGAGAAAGCGAAGGGCAACaggtacg                                              >  1:58977/1‑245 (MQ=255)                                                                                                                                                                                                          gATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTTATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGAGCGTGTGCGTACCGCACTGAAACTAGGTATGGAGCGCGATATCGTTGTTAATAAAGGGAAAGCGCAGGGAAACATGCCCGCCt                                           >  2:124546/1‑250 (MQ=255)                                                                                                                                                                                                          gATGTCAACCGCTACCGAAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCAAGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCGCTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGAGAAAGCGCAGGGCAACATGCCCGCCt                                           >  1:39337/1‑250 (MQ=255)                                                                                                                                                                                                               cacccGCTCCCGTAGGGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTCCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGACCCATCCCTGTGCCCTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGt                                      <  1:93530/247‑1 (MQ=255)                                                                                                                                                                                                               aaaCCTCTACCGTAGTGGGGAAAACGACATGACGAATAACGGCAGGCCGATCGAATTGTTCCAGAAGCTAAAAAAAGAGACCCCGGACGAAGTTCACGTGGACCAAAACCTGTGCATTTACTATTACGAAATAAAAACCCAAAAACCGCCATGCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTAGGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGAAACATGCCCGCCTATGGtt                                     <  1:60428/250‑1 (MQ=255)                                                                                                                                                                                                                          gTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCg                          >  1:16364/1‑251 (MQ=255)                                                                                                                                                                                                                                  aaaaTCGACAGGACAAATAACGGCATGCCGATGGAATTTTTCCAGAAGCTGAAAAAAGAAACCCCGGACAAATTTCTCGTTGATCGATACCGGTCCACTTATTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTCCCGCACTGAAACTAGTTATGGACCGCGATATCATTGTTAATAAATTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGa                  <  1:137003/250‑1 (MQ=255)                                                                                                                                                                                                                                                  aTAACGGCATGCCGATCGACTTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACt  >  2:38632/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      TCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACT  >  NC_000913/1301752‑1302243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑