Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913580,1190AG50.9% 5.3 / 77.9 57intergenic (+33/+132)ybcW/ylcIuncharacterized protein YbcW/DUF3950 domain‑containing protein YlcI
Reads supporting (aligned to +/- strand):  ref base A (11/17);  new base G (13/16);  total (24/33)
Fisher's exact test for biased strand distribution p-value = 7.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCT  >  NC_000913/579870‑580357
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                              
gAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAACTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCACTTGAGGTAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACGGCTAATCATTCAAACTACTTAAc                                                                                                                                                                                                                                               <  2:145954/251‑1 (MQ=255)
      cAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGa                                                                                                                                                                                                                                         <  1:456543/251‑1 (MQ=255)
         aaTGAATAAAGAACCATCTGCTGATGATCCCTCCGAGGATCTGATTCGTGTAAAAAATCTGCTTAATAGCCCCATTTCTATGCGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGTAAGCATTCAGGGCAATTGAGGAAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGGGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGAc                                                                                                                                                                                                                                        >  2:509991/1‑249 (MQ=255)
         aaTGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATTCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGTTTGACTGATCACCATAACTGCAAATCACTCAAACTACGTAACCTGTGACag                                                                                                                                                                                                                                      <  1:172699/251‑1 (MQ=255)
            gAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGc                                                                                                                                                                                                                                   <  2:390583/251‑1 (MQ=255)
            gAATAAAGAACAATCAGCTGATGATCCCTCCGTGGAGCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACagag                                                                                                                                                                                                                                    <  2:491898/250‑1 (MQ=255)
             aaTAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGcc                                                                                                                                                                                                                                  >  1:473622/1‑251 (MQ=255)
                aaaGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCaa                                                                                                                                                                                                                                >  2:138302/1‑250 (MQ=255)
                       aaTCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGt                                                                                                                                                                                                                        >  2:444825/1‑251 (MQ=255)
                            gctgATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTct                                                                                                                                                                                                                      <  1:468401/248‑1 (MQ=255)
                                     cccTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAgg                                                                                                                                                                                                          <  2:36464/251‑1 (MQ=255)
                                       cTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGa                                                                                                                                                                                                         >  1:143626/1‑250 (MQ=255)
                                               aTCTGATTCGTGTAAAACATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACaga                                                                                                                                                                                                                                     >  2:418929/1‑214 (MQ=255)
                                               aTCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGGGACaga                                                                                                                                                                                                                                     <  1:418929/214‑1 (MQ=255)
                                                                             gCACCATTTCTATGAGTGACCCTTATGTTGTAAAAGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGATGCAGCGTGGGTGAAGCACTATAATAATATGAAGGATTAGTCCCTGTTGGTTGACTGATCACCATAACTGCTAATCACTCAAACTACTTAACCTGTGACAGAGCCATCACGCATTCTGTCACTGTCAGGAAAGTGGGAAAACTGCATCTCAAGTACTGCAATGCCCTCg                                                                                                                                                                  <  2:314726/251‑1 (MQ=255)
                                                                                        aTGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTTAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATACAAACTATGTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAg                                                                                                                                                          <  2:500496/248‑1 (MQ=255)
                                                                                               aCCCTGATGTTGGAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACa                                                                                                                                                <  2:278834/251‑1 (MQ=255)
                                                                                                  cTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAat                                                                                                                                              >  2:197703/1‑250 (MQ=255)
                                                                                                   tGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAatat                                                                                                                                            >  1:253573/1‑251 (MQ=255)
                                                                                                         tgtAATTGCATGTATAGAACATAAGGTGTCTCTTGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTcc                                                                                                                                       <  2:464065/250‑1 (MQ=255)
                                                                                                          gtAATTGCATGTATAGAACATCAGGTGTCTCTGGCCGAATTCAGGATAATTGAGGCAGCGTTGGTGAAGCAAGATCATAATATGACGGATTATTACATGGTGGTTGACTGATCACCATAACTGCTAATCATTCAATCTAGTTAACCTGTGACAGAGCCAACAAGACGTCTGTCACTGTCAGGACAGTGGTGAAACTGCAACTCAATTACTGCAATGCCCTCGTATTTAAGTGAATTTACAATATCATCCTg                                                                                                                                     >  2:254418/1‑251 (MQ=255)
                                                                                                            aaTTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGtt                                                                                                                                   >  1:28063/1‑251 (MQ=255)
                                                                                                                     tataGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATCATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCACTTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGga                                                                                                                           >  1:295382/1‑250 (MQ=255)
                                                                                                                             agaaGGAGTCTATGGAAGAAGTCAGGGCAATAGAGGGAGCGTAGGTGAAGGAAGAAAATAATATGAAGGATTATTACCTGGTGGTTGAATGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCAAACACGCAGCCTGTCACTGTCAGGAAAGTGGTAAAACAGCAACACAATTACTGCAATGCAATCGGAATTAAGTGAATTAACAATATCGTAATGTTCGGAGGGCAGAACGCgg                                                                                                                  >  2:136708/3‑251 (MQ=255)
                                                                                                                               aaGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGAGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTACTTAAGTGAATTTACACTATCGTCCTGTTCGGAGGGACGAACGCggg                                                                                                                 >  2:306453/1‑250 (MQ=255)
                                                                                                                                 ggTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCGAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGGCCTGTTCGGAGGGAAGAACGCGGGATg                                                                                                              <  2:253573/251‑1 (MQ=255)
                                                                                                                                          ggAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCAttctt                                                                                                     <  1:316371/251‑1 (MQ=255)
                                                                                                                                           gAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAatat                                                                                                                                            >  2:123146/1‑211 (MQ=255)
                                                                                                                                           gAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAatat                                                                                                                                            <  1:123146/211‑1 (MQ=255)
                                                                                                                                              gCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTtcatc                                                                                                 <  1:306453/251‑1 (MQ=255)
                                                                                                                                                aTTCAGAGCCATTGAGGCAGCGTTGGTGAAGCACGCTCATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTTATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGCAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCAc                                                                                               >  2:435209/1‑251 (MQ=255)
                                                                                                                                                     gggCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACTCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTGCTGCAATGGCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTa                                                                                          <  1:81437/251‑1 (MQ=255)
                                                                                                                                                          attgagcaaGCGTTGGAGAAGCACGATACTAATATGACGGATTAATCCCTGGAGGTTGACTGATCACCATAACTGCTAATCAATCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGCATGTGGGAAAACTGCAACTCAATTACTGCCATGCCCTCGTAATTAAGTGAATTTACAAGATCGTCCAGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCACCACTTTTAATTgg                                                                                     >  2:102775/9‑250 (MQ=255)
                                                                                                                                                          aTTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTg                                                                                      >  1:410515/1‑250 (MQ=255)
                                                                                                                                                              aGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTTGTAAAACTTCAACTCAATTACTGCAATGCCCTCGTAATTAAGGGAATTTACAATATCGTCCTGTTCTGAGGTAAGAATGCGGGATTTTCATTCTTCATCACATTTATTTGATGta                                                                                 <  2:301563/251‑1 (MQ=255)
                                                                                                                                                               ggCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCAGAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCTGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGtat                                                                                <  2:81733/251‑1 (MQ=255)
                                                                                                                                                                     gTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAAACTGAGACAGAGCCAACACGCAGTCTGTCACTGTCAGGCAAGTGGTAAAACTGCAACt                                                                                                                                                                                      >  1:589120/1‑143 (MQ=255)
                                                                                                                                                                     gTTGGTGAAGCACGATAATAATATGAAGGAGTAGTCCCTGGTGGTAGACCGATCACCACAACTGCTAATCATTCAATCTGCGTAACCTGGGACAGAGCCAACACGCAGTTTGTCCCTGTCAGGAAAGTGGAAAAACTGCAACt                                                                                                                                                                                      <  2:589120/143‑1 (MQ=255)
                                                                                                                                                                         gTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCtt                                                                      <  1:542323/251‑1 (MQ=255)
                                                                                                                                                                              gCACGATAATAATATCAAGGATTATTCCCTGGTGGTTGACTGATCACCATACCTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCCGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGCGATGTTCATTCTTCATCGCTTTTAATTGATGTATATGCTCTCTTTTCTg                                                                 <  1:195550/251‑1 (MQ=255)
                                                                                                                                                                                  gATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTCTTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCg                                                                                                                                                                  >  2:527350/1‑150 (MQ=255)
                                                                                                                                                                                  gATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCg                                                                                                                                                                  <  1:527350/150‑1 (MQ=255)
                                                                                                                                                                                        aataTGAATGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAgtc                                                        <  2:121760/250‑3 (MQ=255)
                                                                                                                                                                                        aataTGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCt                                                       <  2:207145/251‑1 (MQ=255)
                                                                                                                                                                                         atatGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCg                                                                                                                                 <  2:35152/176‑1 (MQ=255)
                                                                                                                                                                                         atatGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCg                                                                                                                                 >  1:35152/1‑176 (MQ=255)
                                                                                                                                                                                              aaGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTATCCTCCGACg                                                 <  1:136708/251‑1 (MQ=255)
                                                                                                                                                                                               aGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACGCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCTCTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGTCTCCGACgg                                                <  2:428555/251‑1 (MQ=255)
                                                                                                                                                                                               aGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACGACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTTCTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACgg                                                <  1:560355/251‑1 (MQ=255)
                                                                                                                                                                                                               ggtTTACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATTCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGAccc                                <  1:551854/251‑1 (MQ=255)
                                                                                                                                                                                                                  tGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGTCTCCGACGGCAGGCTTCAATGACCCAgg                             >  1:321011/1‑251 (MQ=255)
                                                                                                                                                                                                                   gACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGTCTCCGACGGCAGGCTTCAATGACCCAgg                             <  2:143626/250‑1 (MQ=255)
                                                                                                                                                                                                                         tCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGTCTTCAATGACCCAGGCTgaga                       >  2:298834/1‑250 (MQ=255)
                                                                                                                                                                                                                             cATAACTGCTAATCAGTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCGTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGCCCCAGGCTGAGAAAtgc                  <  2:28063/251‑3 (MQ=255)
                                                                                                                                                                                                                                  cTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGACGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGTCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAATTTCCCgg              >  2:310530/1‑250 (MQ=255)
                                                                                                                                                                                                                                     cTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACTCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCCTTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGCCGGCAGGCTTCAATGACCCAGGCTGAGACATTCCCGGAccc          >  2:472905/1‑251 (MQ=255)
                                                                                                                                                                                                                                              cAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCGCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACTCGGGATGTTCATTCTTCATCACTTTGAGTGGATGGATATGCTCTCTTTTCGGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCATGCTGAGAAATTCGCGGACCCTTTTTGCt  <  2:310548/250‑1 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                              
GAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCT  >  NC_000913/579870‑580357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑