Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913580,1150CT55.0% 16.2 / 78.5 61intergenic (+29/+136)ybcW/ylcIuncharacterized protein YbcW/DUF3950 domain‑containing protein YlcI
Reads supporting (aligned to +/- strand):  ref base C (10/17);  new base T (15/18);  total (26/35)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCT  >  NC_000913/579869‑580357
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  
ggAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTTCTAATCATTCAAACTATTTag                                                                                                                                                                                                                                                <  1:201373/251‑2 (MQ=255)
 gAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAACTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCACTTGAGGTAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACGGCTAATCATTCAAACTACTTAAc                                                                                                                                                                                                                                               <  2:145954/251‑1 (MQ=255)
     gACAAAATGAATAAAGAACAATCTGCTGATGATCCCACCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGTTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTagtctgt                                                                                                                                                                                                                                           >  2:590756/1‑245 (MQ=255)
       cAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGa                                                                                                                                                                                                                                         <  1:456543/251‑1 (MQ=255)
          aaTGAATAAAGAACCATCTGCTGATGATCCCTCCGAGGATCTGATTCGTGTAAAAAATCTGCTTAATAGCCCCATTTCTATGCGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGTAAGCATTCAGGGCAATTGAGGAAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGGGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGAc                                                                                                                                                                                                                                        >  2:509991/1‑249 (MQ=255)
          aaTGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATTCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGTTTGACTGATCACCATAACTGCAAATCACTCAAACTACGTAACCTGTGACag                                                                                                                                                                                                                                      <  1:172699/251‑1 (MQ=255)
             gAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGc                                                                                                                                                                                                                                   <  2:390583/251‑1 (MQ=255)
             gAATAAAGAACAATCAGCTGATGATCCCTCCGTGGAGCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATACGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACagag                                                                                                                                                                                                                                    <  2:491898/250‑1 (MQ=255)
              aaTAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGcc                                                                                                                                                                                                                                  >  1:473622/1‑251 (MQ=255)
                 aaaGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCaa                                                                                                                                                                                                                                >  2:138302/1‑250 (MQ=255)
                        aaTCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGt                                                                                                                                                                                                                        >  2:444825/1‑251 (MQ=255)
                             gctgATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTct                                                                                                                                                                                                                      <  1:468401/248‑1 (MQ=255)
                                      cccTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAgg                                                                                                                                                                                                          <  2:36464/251‑1 (MQ=255)
                                        cTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGa                                                                                                                                                                                                         >  1:143626/1‑250 (MQ=255)
                                                aTCTGATTCGTGTAAAACATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACaga                                                                                                                                                                                                                                     >  2:418929/1‑214 (MQ=255)
                                                aTCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTagtctgt                                                                                                                                                                                                                                           >  1:255071/1‑202 (MQ=255)
                                                aTCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTagtctgt                                                                                                                                                                                                                                           <  2:255071/208‑7 (MQ=255)
                                                aTCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGGGACaga                                                                                                                                                                                                                                     <  1:418929/214‑1 (MQ=255)
                                                                              gCACCATTTCTATGAGTGACCCTTATGTTGTAAAAGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGATGCAGCGTGGGTGAAGCACTATAATAATATGAAGGATTAGTCCCTGTTGGTTGACTGATCACCATAACTGCTAATCACTCAAACTACTTAACCTGTGACAGAGCCATCACGCATTCTGTCACTGTCAGGAAAGTGGGAAAACTGCATCTCAAGTACTGCAATGCCCTCg                                                                                                                                                                  <  2:314726/251‑1 (MQ=255)
                                                                                         aTGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTTAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATACAAACTATGTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAg                                                                                                                                                          <  2:500496/248‑1 (MQ=255)
                                                                                                aCCCTGATGTTGGAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACa                                                                                                                                                <  2:278834/251‑1 (MQ=255)
                                                                                                   cTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAat                                                                                                                                              >  2:197703/1‑250 (MQ=255)
                                                                                                    tGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAatat                                                                                                                                            >  1:253573/1‑251 (MQ=255)
                                                                                                          tgtAATTGCATGTATAGAACATAAGGTGTCTCTTGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTcc                                                                                                                                       <  2:464065/250‑1 (MQ=255)
                                                                                                           gtAATTGCATGTATAGAACATCAGGTGTCTCTGGCCGAATTCAGGATAATTGAGGCAGCGTTGGTGAAGCAAGATCATAATATGACGGATTATTACATGGTGGTTGACTGATCACCATAACTGCTAATCATTCAATCTAGTTAACCTGTGACAGAGCCAACAAGACGTCTGTCACTGTCAGGACAGTGGTGAAACTGCAACTCAATTACTGCAATGCCCTCGTATTTAAGTGAATTTACAATATCATCCTg                                                                                                                                     >  2:254418/1‑251 (MQ=255)
                                                                                                             aaTTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGtt                                                                                                                                   >  1:28063/1‑251 (MQ=255)
                                                                                                                      tataGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATCATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCACTTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGga                                                                                                                           >  1:295382/1‑250 (MQ=255)
                                                                                                                              agaaGGAGTCTATGGAAGAAGTCAGGGCAATAGAGGGAGCGTAGGTGAAGGAAGAAAATAATATGAAGGATTATTACCTGGTGGTTGAATGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCAAACACGCAGCCTGTCACTGTCAGGAAAGTGGTAAAACAGCAACACAATTACTGCAATGCAATCGGAATTAAGTGAATTAACAATATCGTAATGTTCGGAGGGCAGAACGCgg                                                                                                                  >  2:136708/3‑251 (MQ=255)
                                                                                                                                aaGGTGTCTCTGGAAGCATTCAGAGCAATTGAGGCAGCGTTGGAGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTACTTAAGTGAATTTACACTATCGTCCTGTTCGGAGGGACGAACGCggg                                                                                                                 >  2:306453/1‑250 (MQ=255)
                                                                                                                                  ggTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCGAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGGCCTGTTCGGAGGGAAGAACGCGGGATg                                                                                                              <  2:253573/251‑1 (MQ=255)
                                                                                                                                           ggAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCAttctt                                                                                                     <  1:316371/251‑1 (MQ=255)
                                                                                                                                            gAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAatat                                                                                                                                            >  2:123146/1‑211 (MQ=255)
                                                                                                                                            gAAGCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAatat                                                                                                                                            <  1:123146/211‑1 (MQ=255)
                                                                                                                                               gCATTCAGAGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTtcatc                                                                                                 <  1:306453/251‑1 (MQ=255)
                                                                                                                                                 aTTCAGAGCCATTGAGGCAGCGTTGGTGAAGCACGCTCATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTTATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGCAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCAc                                                                                               >  2:435209/1‑251 (MQ=255)
                                                                                                                                                      gggCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACTCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTGCTGCAATGGCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTa                                                                                          <  1:81437/251‑1 (MQ=255)
                                                                                                                                                           attgagcaaGCGTTGGAGAAGCACGATACTAATATGACGGATTAATCCCTGGAGGTTGACTGATCACCATAACTGCTAATCAATCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGCATGTGGGAAAACTGCAACTCAATTACTGCCATGCCCTCGTAATTAAGTGAATTTACAAGATCGTCCAGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCACCACTTTTAATTgg                                                                                     >  2:102775/9‑250 (MQ=255)
                                                                                                                                                           aTTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTg                                                                                      >  1:410515/1‑250 (MQ=255)
                                                                                                                                                               aGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTTGTAAAACTTCAACTCAATTACTGCAATGCCCTCGTAATTAAGGGAATTTACAATATCGTCCTGTTCTGAGGTAAGAATGCGGGATTTTCATTCTTCATCACATTTATTTGATGta                                                                                 <  2:301563/251‑1 (MQ=255)
                                                                                                                                                                ggCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCAGAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCTGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTTATGtat                                                                                <  2:81733/251‑1 (MQ=255)
                                                                                                                                                                      gTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAAACTGAGACAGAGCCAACACGCAGTCTGTCACTGTCAGGCAAGTGGTAAAACTGCAACt                                                                                                                                                                                      >  1:589120/1‑143 (MQ=255)
                                                                                                                                                                      gTTGGTGAAGCACGATAATAATATGAAGGAGTAGTCCCTGGTGGTAGACCGATCACCACAACTGCTAATCATTCAATCTGCGTAACCTGGGACAGAGCCAACACGCAGTTTGTCCCTGTCAGGAAAGTGGAAAAACTGCAACt                                                                                                                                                                                      <  2:589120/143‑1 (MQ=255)
                                                                                                                                                                          gTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCtt                                                                      <  1:542323/251‑1 (MQ=255)
                                                                                                                                                                               gCACGATAATAATATCAAGGATTATTCCCTGGTGGTTGACTGATCACCATACCTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCCGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGCGATGTTCATTCTTCATCGCTTTTAATTGATGTATATGCTCTCTTTTCTg                                                                 <  1:195550/251‑1 (MQ=255)
                                                                                                                                                                                   gATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTCTTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCg                                                                                                                                                                  >  2:527350/1‑150 (MQ=255)
                                                                                                                                                                                   gATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCg                                                                                                                                                                  <  1:527350/150‑1 (MQ=255)
                                                                                                                                                                                         aataTGAATGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAgtc                                                        <  2:121760/250‑3 (MQ=255)
                                                                                                                                                                                         aataTGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCt                                                       <  2:207145/251‑1 (MQ=255)
                                                                                                                                                                                          atatGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCg                                                                                                                                 <  2:35152/176‑1 (MQ=255)
                                                                                                                                                                                          atatGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCg                                                                                                                                 >  1:35152/1‑176 (MQ=255)
                                                                                                                                                                                               aaGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTATCCTCCGACg                                                 <  1:136708/251‑1 (MQ=255)
                                                                                                                                                                                                aGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACGCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCTCTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGTCTCCGACgg                                                <  2:428555/251‑1 (MQ=255)
                                                                                                                                                                                                aGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACGACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTTCTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACgg                                                <  1:560355/251‑1 (MQ=255)
                                                                                                                                                                                                                ggtTTACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATTCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGAccc                                <  1:551854/251‑1 (MQ=255)
                                                                                                                                                                                                                   tGACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGTCTCCGACGGCAGGCTTCAATGACCCAgg                             >  1:321011/1‑251 (MQ=255)
                                                                                                                                                                                                                    gACTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGTCTCCGACGGCAGGCTTCAATGACCCAgg                             <  2:143626/250‑1 (MQ=255)
                                                                                                                                                                                                                          tCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGTCTTCAATGACCCAGGCTgaga                       >  2:298834/1‑250 (MQ=255)
                                                                                                                                                                                                                              cATAACTGCTAATCAGTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCGTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGCCCCAGGCTGAGAAAtgc                  <  2:28063/251‑3 (MQ=255)
                                                                                                                                                                                                                                   cTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGACGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGTCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAATTTCCCgg              >  2:310530/1‑250 (MQ=255)
                                                                                                                                                                                                                                      cTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACTCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCCTTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGCCGGCAGGCTTCAATGACCCAGGCTGAGACATTCCCGGAccc          >  2:472905/1‑251 (MQ=255)
                                                                                                                                                                                                                                               cAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCGCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACTCGGGATGTTCATTCTTCATCACTTTGAGTGGATGGATATGCTCTCTTTTCGGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCATGCTGAGAAATTCGCGGACCCTTTTTGCt  <  2:310548/250‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  
GGAGCGACAAAATGAATAAAGAACAATCTGCTGATGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAGTTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGGGCAATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATCACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGCCTCCGACGGCAGGCTTCAATGACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCT  >  NC_000913/579869‑580357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑