Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (+589/+167)	rtcR → / ← glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	237.9 / NA	67	intergenic (+589/+167)	rtcR/glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (26/41);  total (26/41)

ATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGC  >  NC_000913/3560205‑3560696                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  aTCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGATGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGCACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGc                                                                                                                                                                                                                                                    >  2:503268/1‑251 (MQ=255)   cttcttTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGcgc                                                                                                                                                                                                                                                  >  1:95029/1‑249 (MQ=255)   cttcttTTACCATCAAAGTGTTAGCCACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGcgc                                                                                                                                                                                                                                                  >  2:494787/1‑249 (MQ=255)           cccaccaaggtgtTAGCAACGTGGAGATTGTGGGTGACAATGCGAAAATTGCTGTGATGGAGCAGGGCGTGCCCTACCGCTGCCGGGGGGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCT‑CCGCCATGATGGCGCAGGATCAg                                                                                                                                                                                                                                          <  1:405081/242‑1 (MQ=255)           aCCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATc                                                                                                                                                                                                                                            >  1:418996/1‑249 (MQ=255)                cAACGAGTTAGCAAAGTTGCGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCAGATATCGATAAACAGCGTCGCGCCATTGGGG‑ATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGtttt                                                                                                                                                                                                                                     >  2:533461/1‑251 (MQ=255)                     tgtTAGCAACGTTGAGATGGTTGGGGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGCTAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCACCATGTTGGCGCAGGATCAGGTTTTGCTcc                                                                                                                                                                                                                                >  2:506315/1‑251 (MQ=255)                                       tgtgggTGACAATGCGAAAATTGCTGTGATTGAGCTGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATACCGATAAACGGCGGCGAGCCATGGGGG‑ATTTGCTCCGCCACTTTGCGGGCGATGCGCGCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCGCATTGAGGTc                                                                                                                                                                                                               <  1:178619/246‑1 (MQ=255)                                             tGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggcg                                                                                                                                                                                                         >  1:502143/1‑250 (MQ=255)                                             tGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggcg                                                                                                                                                                                                         >  1:515780/1‑250 (MQ=255)                                                       cAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCg                                                                                                                                                                                              >  1:128413/1‑251 (MQ=255)                                                        aaaTTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGGGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCgg                                                                                                                                                                                             <  1:321183/251‑1 (MQ=255)                                                         aattgttatgATTGAGCAGAGCGTGCGCTACCCCTTCCGCCGTGGCGCCGATCTCGATAAACAGCGTCGAGAACTTGGGG‑ATTTGCTCCGCCCCTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGCAGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAAACAAACTGGAAGGCAGTGCCGCA‑CCGCCATCATGGCGCAGAAACAGGATTTGCTCCGCCAGATCAATGAGGTCGCGGCGAATCGTCTGCggg                                                                                                                                                                                            >  2:596278/9‑251 (MQ=255)                                                         aagtgcgtggtttgggCAGGGCGTGCGCTCCCGTTTCCGGGGGGGGGCCGATAGCGATAAACAGCGTGGAGCCAGTGGGG‑TTTGGCCCCGCCCCTTTGCGGGCGAGGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCggg                                                                                                                                                                                            <  2:178381/236‑1 (MQ=255)                                                               tgtgATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGACGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACg                                                                                                                                                                                      >  2:5737/1‑251 (MQ=255)                                                                gtgATTGAGCAGTGCGTGCGCTACCGCTCCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACg                                                                                                                                                                                      <  1:131604/250‑1 (MQ=255)                                                                  gATTGAGCAGTGCGTGCGCTCCCGCTCCCGGCGTGGTGCCGGTATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCGGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACGGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGc                                                                                                                                                                                                                                   <  1:191678/203‑1 (MQ=255)                                                                  gATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGGTATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGc                                                                                                                                                                                                                                   >  2:191678/1‑203 (MQ=255)                                                                     tgagccgtgcacgcgcTACCGATTCCGGCGTGGTGCCGATATCGATAAAAAGCGTCGAGCCATTGGGG‑ACTTGCTCCGCCACTTTGCGGGCGATGCGCGCTTTTTCTTCG‑GTCGGGGTGGCCTTGCGAT‑CGTGCCACGGCGCGT‑TAACCAAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGATTTGCTCCGACAGCTCATTGAGGTCGCGGAGAATAGACTG‑GGGCAGACgtgaaaa                                                                                                                                                                                >  2:76565/13‑244 (MQ=255)                                                                                      cccGCTTCCGGCGGGGGGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctct                                                                                                                                                                 <  1:20445/248‑1 (MQ=255)                                                                                         gCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGATCTTCGGt                                                                                                                                                            >  1:38964/1‑251 (MQ=255)                                                                                                 ggtggggCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑TTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACAt                                                                                                                                                    <  1:127374/250‑1 (MQ=255)                                                                                                 cGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACAt                                                                                                                                                    >  1:164858/1‑251 (MQ=255)                                                                                                         ggATATCGATAAACAGCGCCGGGCCATTGGGG‑ATTGTCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTGCG‑GTCTGGGTGGCCTTGCGAT‑CGGGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgc                                                                                                                                            <  1:291069/250‑1 (MQ=255)                                                                                                         caatatAGATAAACAGCGTCGAGCCATGGGGG‑ATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑TTCTGGGTGGCTTTGCGAC‑CGTGCCACGGCGTTT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgc                                                                                                                                            <  1:103427/249‑1 (MQ=255)                                                                                                           atatctaaaaaCAGCGTAGAGC‑ATTGGGGTATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGTCTTTT‑AAACCGAACTGGAAGGCAGCCCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgct                                                                                                                                           <  1:84859/242‑1 (MQ=255)                                                                                                              cggATAAACAGCTTCGAGCCATTGGGG‑ATTGTCTCCGCCTCTTGGCGGTCGATGCGCTCTTTTTCTTCG‑GCATGGGGGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TACCCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGttt                                                                                                                                       <  1:70763/249‑1 (MQ=255)                                                                                                                   aaaCAGCGTCGAGCCATTGGGG‑ATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAcc                                                                                                                                   >  1:469433/1‑250 (MQ=255)                                                                                                                        ggcgcgggcccttgggggttgtcgcccgccgCTTTGCGGGCGATGCGTTCTTTTTCTTCGTGT‑TGGGTGGCCTGGCGAT‑CGTGCCACGGCGTGT‑TAACCAAACTGGAAGGCGGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTTACATAACCCTGCTGTTTAACCAGTTc                                                                                                                              <  2:540996/226‑1 (MQ=255)                                                                                                                        gCGTCGAGCCATGGGGG‑ATTGGCCCCGCCACTGTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCg                                                                                                                             <  1:18683/251‑1 (MQ=255)                                                                                                                           tCGAGCCATTGGGG‑TTTTGCTCCGCCACTTTGCGAGCGCGTCGCTCTTTTTCTTCG‑GTCTGGGAGGCCTTGCGAT‑CGTACCCCGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCCTGAGGGCGCAGGAAACGGTTTTGCTCCGCCAGCTCATGAAGGTCCCGGCGACTAGACTGCGGGCTGACGGAGAAATGCTCTAACAGCTCTACGGTACGAACATAACCCTGCTGTTTAACAAGTTCGat                                                                                                                           >  2:551333/1‑250 (MQ=255)                                                                                                                             cccctctcttatcttttGCTCGTCCACTGTGCGGGCGATGCGCTCTGTTTCTTCG‑GTCTGGGGGGCCT‑GCCATTCGTGCCCCGGCGGGT‑AACCCGACCGGGAAGGCCGCCCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACGGACATAACCCTGCTGTTTAACCAGTTCGataa                                                                                                                         <  2:574252/237‑1 (MQ=255)                                                                                                                               gCCATTGGGG‑CTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CAGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATAc                                                                                                                      >  2:391217/1‑251 (MQ=255)                                                                                                                                   ttGGGG‑ATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGtt                                                                                                                  >  1:523778/1‑251 (MQ=255)                                                                                                                                    ttgtggttttgtaccgacccTTTGCGGGCGATGCGCTCTTATTCTTCG‑GTCGGGGTGGCCTTGCGGT‑CGTGCCACGGTGTGT‑TAACCGACTTGGAAGGAAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGGTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACAAAACCCTGCTGTTTACCCAGTTCGATAATACCg                                                                                                                    <  1:510526/230‑1 (MQ=255)                                                                                                                                    ggggg‑ATTTGCTCCGCCCCTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtg                                                                                                                 <  1:391217/250‑1 (MQ=255)                                                                                                                                           tttgtccctctcgtttgagtgctaggcgcTCTTTTTTTTCG‑TCCTGGGGGGCTGTGTGAT‑CTTGCCACGTCGTGT‑TAACCGATTGGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTTCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACg                                                                                                            <  1:339320/225‑1 (MQ=255)                                                                                                                                           tttGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCCGGATCAGATTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACAAGTTCGataa                                                                                                                         >  1:574252/1‑237 (MQ=255)                                                                                                                                               cTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TACCCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGGTCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTtgtg                                                                                                       >  1:588259/1‑251 (MQ=255)                                                                                                                                               cTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCCGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCAGCTGTTTAACCAGTCCGATAATACCGTTGTGACGtattg                                                                                                       >  2:17249/1‑247 (MQ=255)                                                                                                                                                  ccccccTTTGCGGGCGATGCGTTCTTTTTCTTCG‑GCGTGGGGGGCCTTGCGTT‑CGTGCCACGGCGGGT‑TAACCGAACGGGAAGGCGGCCCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgttt                                                                                                    <  2:128413/246‑1 (MQ=255)                                                                                                                                                   gCCACTTTGCGGGCGAGGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttg                                                                                                   <  1:533461/251‑1 (MQ=255)                                                                                                                                                   gCCACTTTCCGGGCGACGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGAGCAGGTTTTGCTCCGCCAGCTCATTGAGGTCTCGTCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGTAATAACCCTGCTGTTTAACCAATCCGATACTACCGTTGAGACGTTCtgtttg                                                                                                   >  2:520535/1‑251 (MQ=255)                                                                                                                                                         ttGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCAAGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTc                                                                                               <  1:147852/249‑1 (MQ=255)                                                                                                                                                           gCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGGGT‑TAACCGACCTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCAttt                                                                                           <  1:114288/251‑1 (MQ=255)                                                                                                                                                                 gaggcgcTCTTTTTCTTCG‑GTCTGGGTGGCCTGGCGAT‑CGGGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAc                                                                                                                                                 <  1:480422/188‑1 (MQ=255)                                                                                                                                                                 gATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAAc                                                                                                                                                 >  2:480422/1‑191 (MQ=255)                                                                                                                                                                   tGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATcc                                                                                   >  1:184713/1‑251 (MQ=255)                                                                                                                                                                      gcTCTTTTGCTTCG‑GTCGGGGTGGCCTTGCGAC‑CGTGCCACGGCGTGT‑TAACCGAACTGGAGGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTg                                                                                <  2:33092/251‑1 (MQ=255)                                                                                                                                                                          tttttccttggggcTGGGTGGCCTTGCGAT‑CGTGCCGCGGCGTGT‑TAACCGAACGGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGTAGAAATGCTCTACCAGCTCTTCGGGACGGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTgg                                                                               <  2:84963/236‑1 (MQ=255)                                                                                                                                                                            tttCTGCG‑GTCGGGGGGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTAGAAATCCCTGGAatt                                                                           <  1:444998/250‑1 (MQ=255)                                                                                                                                                                             ttcttcG‑GTCTGGGTGGCCTTGCGAT‑CGGGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAatta                                                                          <  1:5737/250‑1 (MQ=255)                                                                                                                                                                              ttcttgggtggggggggcttttggAT‑CGTGCCACTGCGTGT‑TAAACAGACTGGAAGGCAGCGGCCCC‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTTACGGATAAATGCTCTACCAGCTCTTCGGTTCTGACATAACCCTGCTGTTTAACCAGTTCGATAATGCCGTTGTGACGTTGTGTTTGTGTCATTTATAAATCCCTGGAatta                                                                          <  2:497444/227‑1 (MQ=255)                                                                                                                                                                                 tcG‑GTCTGGGTTGCTCTGCTGT‑CGTGCCCCGGCTGGT‑TATCTGAACGGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACTAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAattatt                                                                        <  1:207652/248‑1 (MQ=255)                                                                                                                                                                                  tcgtggtGGGGGGCCTTGCGAT‑CGTGCCACGGCG‑GTATACCCGAACTGGAGGGCAGCCGCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAtttt                                                                      <  2:37880/244‑1 (MQ=255)                                                                                                                                                                                    gggctggtgggCCTTGCGAT‑CGTGCCACGGTGTGT‑TAACCGAACGGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgt                                                                   <  1:11044/242‑1 (MQ=255)                                                                                                                                                                                        ggggTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGAAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttttc                                                               <  1:503268/250‑1 (MQ=255)                                                                                                                                                                                         gggTGGCCTTGCGCT‑CGGTCCCCGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGTATCAGGTTTTGCTCCGCCAGCGCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttttc                                                               <  1:451854/250‑1 (MQ=255)                                                                                                                                                                                           gTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTcgcg                                                            >  1:459960/1‑251 (MQ=255)                                                                                                                                                                                            gggCCTTGCGTC‑CTTGCCCCGGCGTGT‑TAACCGAACTGGAAGGCATCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCgcgc                                                           <  2:502143/250‑1 (MQ=255)                                                                                                                                                                                                 ttGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCAtt                                                        <  1:492750/249‑1 (MQ=255)                                                                                                                                                                                                  tgctaT‑CGTGACGCGGCGTTT‑TAACCGAACTGGAAGGCAGCGCGGCT‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGa                                                      <  2:538909/246‑1 (MQ=255)                                                                                                                                                                                                          gTGCCCCGGCGTGG‑TAAACAAAGTGGAGGGCAGCGC‑GCAACCGCAATGATGGCGCAGGATCAGGTTTTGCGCCGGCAGCTCATTGAGGGCGCGGCGAATAGTCTGCGGGCTGCCGGAGAAATGCTCTACCAGCGCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTGTGTGTCATTGATAAAACCCTGTAATTATTGGCGTTTTCGCGTATTGAGCGAATca                                              <  2:588259/251‑1 (MQ=255)                                                                                                                                                                                                           gtcccgagCGTGT‑TAACCGAACTGGAAGGCAGCCCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCATCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAAGCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaa                                             <  2:459960/244‑1 (MQ=255)                                                                                                                                                                                                              cACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaaca                                           >  1:543158/1‑250 (MQ=255)                                                                                                                                                                                                                                    gcaGGCATCGCCGAA‑CCGCCATGATGGCGCAGGATCAGTTTTTGCTCCGCCATCTCATTGAGGTCGCGGCGAAGATTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTTACATAACCCTGCTGTTTAACCAGTTCGATAATACCTTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACgg                     <  2:473361/249‑1 (MQ=255)                                                                                                                                                                                                                                      gggCAGCGCCGCA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCt                   <  2:515780/250‑1 (MQ=255)                                                                                                                                                                                                                                             gccgcA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCgg            <  1:494787/251‑1 (MQ=255)                                                                                                                                                                                                                                               cgcA‑CCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCg          >  1:177609/1‑251 (MQ=255)                                                                                                                                                                                                                                                        cATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGc  >  1:388261/1‑251 (MQ=255)                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  ATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGG‑ATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCG‑GTCTGGGTGGCCTTGCGAT‑CGTGCCACGGCGTGT‑TAACCGAACTGGAAGGCAGCGCCGCA‑CCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGC  >  NC_000913/3560205‑3560696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑