Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,926,543 2 bp→TT coding (236‑237/1365 nt) ppnN → nucleotide 5'‑monophosphate nucleosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,926,5430CT96.2% 76.8 / ‑3.5 26P79L (CCC→CTT) ppnNnucleotide 5'‑monophosphate nucleosidase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (13/12);  total (14/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01
*NC_0009132,926,5440CT96.2% 76.5 / ‑3.5 26P79L (CCC→CTT) ppnNnucleotide 5'‑monophosphate nucleosidase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (13/12);  total (14/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01

TTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCT‑GGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGT  >  NC_000913/2926303‑2926775
                                                                                                                                                                                                                                                 ||                                                                                                                                                                                                                                       
ttttCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCT‑GGAACTGATTAATCTTCCGGAag                                                                                                                                                                                                                                  >  1:238158/1‑249 (MQ=255)
                        cGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCT‑GGCACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAattatt                                                                                                                                                                                                        >  1:137307/1‑251 (MQ=255)
                              gCTCCATGGATATTTGGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCT‑GGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCt                                                                                                                                                                                                  <  2:267612/251‑1 (MQ=255)
                                 ccATGGATATGTTGTCGCAGCTGGAAGCGGCTATGCTTAAACGCAACGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCT‑GGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTg                                                                                                                                                                                               >  2:147021/1‑251 (MQ=255)
                                   agggATATGTTGTCGCAGCTGGAAGTGGATTTGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCT‑GGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCa                                                                                                                                                                                             <  1:50955/249‑1 (MQ=255)
                                         atGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGCTATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCT‑GGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGAGCTTTGCAGGCCa                                                                                                                                                                                        >  1:173608/1‑250 (MQ=255)
                                           gttgtCGCAGCTGGAAGGGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCT‑GGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAAc                                                                                                                                                                                      <  2:238158/250‑1 (MQ=255)
                                              gtCGCATCTGAAAGTGGAATTGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGGCTGGCGCCGTGAACGCGGCGTAAAGCT‑GGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCGACTTGt                                                                                                                                                                                  <  2:43153/251‑1 (MQ=255)
                                              gtCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCT‑GGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTg                                                                                                                                                                                   <  1:180596/250‑1 (MQ=255)
                                               gcGCAGGTGGAAGGAGATATGCTTAAACGCACCGCCAGCAGCGACCTCTAGCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCT‑GGAACGGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCTAACTTGtt                                                                                                                                                                                 <  1:138384/250‑1 (MQ=255)
                                                         aaGTGGAAAGGCTTAAACCCACCGCCAGCAGCGTCCTCTATCAACTGGTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCT‑GGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTGTGCAGGCCAACTTGTTCGCTGTCCt                                                                                                                                                                        <  2:328035/250‑1 (MQ=255)
                                                              gATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTAGCAACTGTTCACTTGCCGTACTGAAATCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGCTATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCT‑GGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTg                                                                                                                                                                   >  1:170338/1‑250 (MQ=255)
                                                                   tcTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGATCTCCGGTAGTTTGTCCGATAACAGCAAAGAATTGCTGTCTCGGTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCT‑GGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGATTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACAtt                                                                                                                                                              <  1:237157/249‑1 (MQ=255)
                                                                                       gcGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCT‑GGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAAGGATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATc                                                                                                                                         <  1:325139/251‑1 (MQ=255)
                                                                                                             cAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCT‑GGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCa                                                                                                                   <  1:132451/251‑1 (MQ=255)
                                                                                                                     cACTTGCCGTACTTAACTCCGGTAGTTTGACCGATAACAGCAAAGAAGTGCTGTCTCGTTTTTAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCG‑GGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGGTAGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGGTCGTTTTCCCAACCTTAAt                                                                                                           <  2:765/251‑1 (MQ=255)
                                                                                                                                         ggTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCT‑GGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGAGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCACCCTGAATCTCGACAACTCCGTCCACAt                                                                                       >  1:169635/1‑251 (MQ=255)
                                                                                                                                                tgactgttAACAGCAAAGAATGGCTGTCTCGTTTTGAAAATTTCGATATTAATGTCTTGCTCCGTGACCGCTGCGTAAAGCT‑GGAACTGATTAATCTTCCGGAAGAGGTTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTTCGTGACATTCTCTTCGTTTCCGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCTCTat                                                                                 <  2:8223/243‑2 (MQ=255)
                                                                                                                                                tGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCT‑GGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAAc                                                                                >  1:141488/1‑251 (MQ=255)
                                                                                                                                                                aGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCT‑GGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGAGCTTTGCAGGCAAACTTGTTCGCGGGCCTGCGTGACATTCTCTTCGTTTACAGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATTTCTACAACTCCGTCCACATCACTAACCTGGTCTTTTCAATCt                                                                >  1:227060/1‑251 (MQ=255)
                                                                                                                                                                                                     gTCTGGCGCGGTGAACCCGGAGTAAAACTTGGAA‑TGATTAATCTTCCGGAAGAGGCTTTTGTCGATAGGCGAATTATTCGAGCATTGCAGGCCAACTAGGTCGAGGTGCTGCGGGACATTCTCTTCGTTTACGGGCACATACATAACACCGTTGGTTTTCCCAACCGGAATTTCGACAACTCCGTCCCCATCACTAACCTGGTCTTTTCCATCTTGCGTCACGCCCGCGCTCTGCATGTGGGTGAAGCGcc                           >  2:197225/1‑251 (MQ=255)
                                                                                                                                                                                                           cgcCGTGAACGCGGCGTAAAGCT‑GGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATAACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAatat                     >  1:293435/1‑251 (MQ=255)
                                                                                                                                                                                                              cGTGAACGCGGCGTAAAGCT‑GGAACTGATTCATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGACTCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGAGGGTGAAGCGCCAAATAtggt                  >  1:321105/1‑251 (MQ=255)
                                                                                                                                                                                                                 gAACGCGGCGTAAAGCT‑GGAACTGATTAATCTTACGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTCAAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTAGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCCACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCACGCGCTGCATGTGGGTGAAGCGCCCAATATggtggt               >  2:237037/1‑251 (MQ=255)
                                                                                                                                                                                                                  aaCGCGGCGTAAAGCT‑GGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTCCGGGCACATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTc              >  1:350182/1‑251 (MQ=255)
                                                                                                                                                                                                                              aGCT‑GGAACTGATTAATCTTCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGAGGt  >  2:18151/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 ||                                                                                                                                                                                                                                       
TTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCT‑GGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGT  >  NC_000913/2926303‑2926775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑