Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,630,295	C→T	Q76Q (CAG→CAA)	ettA ←	energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,630,295	0	C	T	100.0%	118.9 / NA	38	Q76Q (CAG→CAA)	ettA	energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/22);  total (16/22)

CGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTG‑CGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCAC  >  NC_000913/4630049‑4630522                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                     cgcgTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATTACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑Cg                                                                                                                                                                                                                                 <  2:229943/251‑1 (MQ=255)    gTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCa                                                                                                                                                                                                                              <  1:283180/251‑1 (MQ=255)          gTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAAGCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAACCCGCTTCTTCAATGGACTCACGCACGGTGTGGTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGATAAc                                                                                                                                                                                                                        <  2:154540/251‑1 (MQ=255)             cGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCATCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGTCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACGCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGACAACCaa                                                                                                                                                                                                                     <  1:40904/251‑1 (MQ=255)                     gTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGCCCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGATAACCAATCTTGATg                                                                                                                                                                                                             >  2:230171/1‑251 (MQ=255)                       aGCGCGTCCGCCGCACGCTCCAGCTGTACGTTCCGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCCTACACTTCCTCCAGGCGTTTCAGGGCGTTAACCAATTCTGACACGGCTTCTTCACTGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGATAACCAAGCTTGTTGTc                                                                                                                                                                                                           >  2:313426/1‑251 (MQ=255)                           cATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGATAACCAATCTTGATGTCTGGc                                                                                                                                                                                                       <  1:333723/251‑1 (MQ=255)                                    cACGCTCCAGCTGTACGTTCCGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGACTTGTTCAGCGGCCAGCTTGTCAAACTCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTACCCACTTCTGAAACCGCTTCTTCAATGGCCTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGATAACCACTCTTGATGTCTGGCTGCGGAcgg                                                                                                                                                                                              >  2:34999/1‑250 (MQ=255)                                          ccAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGTCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTc                                                                                                                                                                                         <  1:23409/250‑1 (MQ=255)                                                gTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCg                                                                                                                                                                                  <  1:112536/251‑1 (MQ=255)                                                   cGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCGTCGATg                                                                                                                                                                               <  2:58534/251‑1 (MQ=255)                                                            tATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAGCCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCGGCGGTTCTTG‑CGGCAGATAACCAATCTTGATTTCTGGCTGCGGACGCGCTTCGCCTTCGATGTCTTTATc                                                                                                                                                                       <  2:145618/250‑1 (MQ=255)                                                                  cgtcgtGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGATAACCaa                                                                                                                                                                                                                     <  1:313898/198‑1 (MQ=255)                                                                  cgtcgtGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGATAACCaa                                                                                                                                                                                                                     >  2:313898/1‑198 (MQ=255)                                                                   gtcgtGAGCCTGAATGATCACTTTCAGACGGCCTTGCTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATTGGCGTACAGCGCATACACTGCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGATAACCAAGCTTGATGGCGGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCg                                                                                                                                                               <  1:342555/251‑1 (MQ=255)                                                                       tGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCAt                                                                                                                                                           >  1:118846/1‑251 (MQ=255)                                                                                   aTCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCCCTTCTGACACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGATAACCAATATTGATGTCTGGCTGCGGGCGCGCTTCACCTTCGCTGTCTTTATCAATGCCCGCCATAATGCgcagc                                                                                                                                                 >  1:325877/1‑249 (MQ=255)                                                                                       cttcctgcgggcttttgtCAGCGGCCAGTTTGTCAAAAGCGGTATCCGGATCGGCGTACGGCCCAGACTCTTCACCCAGGCGTTTCAGGGCGTTACCCCCTTCTGAACCCGCTTCTTAAATGGACTCACGCACGGTGTTTTCCGGTTTCAGCTGCGGTTTTGG‑CGGCAGTTAACCATTCTTGATGTCTGGCTGCGGACGCTCGTCCCCTGCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAgg                                                                                                                                              <  1:146823/231‑1 (MQ=255)                                                                                                gtctgtttccgggggCCAGTTTGTCAAAAACGGACTCCGGATCGGCTTACAGCCCATCCACTTCCTCCAGGCGTTTCAGGGCGTTATCCCTTTCTGAAACCGCTTCTTCATGGGACTCACGCAGGGTGTGTTCCGGTTTCAGCTGCGGTTTTTG‑CGGAAGATAACCATTCTTTAGGTCTGGCTGCGTACGGGCTTCACCTTCGATGTTTTTTTCAATCCCCGCCATAATGCGCAGCAGGGTGGACTTAccc                                                                                                                                  <  1:170903/238‑1 (MQ=255)                                                                                                gtccTTGTTCCTCGGCCTGCTTTTCAAAATCGCCATCCGGTCCGGCGTACAGCGCATACCCTCCACCCAGGCTTTTCAGGCCGTTAACCTCTTCTGAACCCTCTTCTTCAATGGCCTCCCGCACGTTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGATACCCAATCTTGATGTCTGGCTGCGGCCGCGCTCCCCCTTCGATGTTTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTAccc                                                                                                                                  <  1:170890/249‑1 (MQ=255)                                                                                                ggCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTAccc                                                                                                                                  >  1:177982/1‑251 (MQ=255)                                                                                                 cctttGTTCAGCGGCACGCTTGCAAAAATCGGCATCGGGATCGGCGTACAGCGAATACACTCCATCCAGGCGTTTCAGGGGTTTAACCACTTCGGAACCCGCTTCTTCAAGGGCCTCCCGCCCGGTGTGTGCCGGGTTCAGCGGCTGTTCTTG‑CGGAAGATAACCATTCTGGAGGTCGGGCGGCGGACGCGCTTCCCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCcg                                                                                                                                 <  1:207218/248‑1 (MQ=255)                                                                                                    ttGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCgcgc                                                                                                                              >  2:8789/1‑251 (MQ=255)                                                                                                         cAGCGGCCACCTTGACAAAAGCGTCACCCGGATCGGCGTACGGCGAATACACTCCACCCAGGCGTTCCAGGGCGTTACCCACTTTTAAAACCTCGTCTTAAAGGGCCTCACGCCCGGTGTGTCCCGGGTCAAGCGGCGGTTCTGG‑CGGCAGATAACCAATCTTGTTGCCGGGCTGGGGACGCGCTTCCCCTTCGATGTCTTTATCAAGGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTc                                                                                                                         <  1:7/251‑1 (MQ=255)                                                                                                           ccGGCCATCTTCTCAAAATCGGTATCCGGATCGGCTTCCAGCGCACACTCTTACCCCAGGCTTTTCAGGGCGTTACCCCCTTCTGAAACCGCTTCTACAATGGACTCACGTACGGTGGGTCCCGGGTTCAGCTGCGTTTCTTG‑CGGCAGATACCCAATCTTGATGTCTGGCTGAGGCCGCGTTTCCCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAg                                                                                                                       <  1:69277/250‑1 (MQ=255)                                                                                                            cGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCACTGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTc                                                                                                                         >  1:181290/1‑248 (MQ=255)                                                                                                                           aaTCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAAtt                                                                                                       >  2:304905/1‑251 (MQ=255)                                                                                                                                         ccggcgtaccgagcttaccctccctCCAGGCTTTTCAGGGCGTTAACCCCTTCTGAACCCTTTTCTTCAATGGCCTCACGCCCGGTGTGTTCCGGGTTCAGCGGCGGTTCTTG‑CGTCAAATAACCAATCTTGTGGTCGGGCTGCGGACGCGCTTCCCCTTCGATGTTTTTATCAATGCCCTCCATAATGCGCAGCAGGGTGGACGTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGgaa                                                                                         <  1:197084/227‑1 (MQ=255)                                                                                                                                         ccGGCGTACAGCGCATACCCTACACCCTGGCGTGTCAGGGTGTAACCCTCTTCTAAACCCGCTTTTTCAAGGGACTCACGCAGGGTTTGTTCCGGGTTAAGCGGCGGTTCTGG‑CGGCAAATAACAAATCTTGAGGTCTGGCTGCGGACGCGCTTCTCCTGCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGga                                                                                          <  2:110945/249‑1 (MQ=255)                                                                                                                                                              tCATCCAGGCGTTTCAGGGCGTTAAACACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGAGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACCCCACTTTTTGCCCCAGGGAAGAAACTCAGCGAGATGttttt                                                                    >  2:25052/1‑251 (MQ=255)                                                                                                                                                                             aGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGttt                                                     >  1:174915/1‑251 (MQ=255)                                                                                                                                                                                                  aCCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGac                                >  1:306822/1‑251 (MQ=255)                                                                                                                                                                                                    cGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGacac                              >  1:48414/1‑251 (MQ=255)                                                                                                                                                                                                               ggtccacacccctggTGTGTTCCGGGTTCAGGTG‑GGTTTTTGGAGGCAGATAACCAATTTTGATGTCGGGCGGGGGCCGCGCTTCACCTTCGATTTCTTTACCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGta                   <  2:307882/238‑1 (MQ=255)                                                                                                                                                                                                                  aCTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGtata                 >  1:320103/1‑250 (MQ=255)                                                                                                                                                                                                                    ccacgctcgGTGTGTTCCGGGTTCAGCTGCGGTTCTGG‑CGGCAGATAACCAATCTTGATGTCTGGCGGCGGCCGCGCTTCCCCTTCGATGTCTTTACCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAAc              <  1:106563/250‑1 (MQ=255)                                                                                                                                                                                                                          acgGTGTGTTCCGGGTTCAGCTGCGGTTCTTG‑CGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTg        >  2:139548/1‑251 (MQ=255)                                                                                                                                                                                                                                 ggtCCGGGTTCGGCGGCGGTTCTTG‑GGGCAGATAACAAATTTTGATGCCTGGGTGCGGACGCCCTTCACCTTCGATGTCTTTATAATTGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCAc  <  2:174915/248‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                     CGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTG‑CGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCAC  >  NC_000913/4630049‑4630522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑