Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,587,365	C→T	S139S (AGC→AGT)	mrr →	Type IV methyl‑directed restriction enzyme EcoKMrr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,587,365	0	C	T	100.0%	129.1 / NA	43	S139S (AGC→AGT)	mrr	Type IV methyl‑directed restriction enzyme EcoKMrr
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/24);  total (19/24)

GGCGCATGACCGTTTAAAACGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGCCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGC  >  NC_000913/4587131‑4587607                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   ggCGCATGACCGTTTAAAACGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGCGCAGTCCGGACGATCGGtt                                                                                                                                                                                                                                      >  2:121194/1‑249 (MQ=255)   ggaatggccGTGTAAAACGTGCCGGGTTGTCGCAAAGTTGGTCGCGTGGCAAAGGGTGCCTGACTCATGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACAAGCTGGCCTTCGCTTTTGGGAATGTCAAACTTAAGTCACGGCCGGAGGCCGTCGATTGAGATCCGAAAGCCGACTCTCCCGATCAGGAAGAACTTGCAAAGAGCAGTCAGGACGATCGGTTAGAt                                                                                                                                                                                                                                  <  2:184343/244‑1 (MQ=255)                     gTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCtt                                                                                                                                                                                                                >  1:137172/1‑251 (MQ=255)                         cGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCAACTGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGCTTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCACAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGCGCTTCGTg                                                                                                                                                                                                            >  2:217225/1‑251 (MQ=255)                           ggTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGATTCGTGAt                                                                                                                                                                                                          >  1:227779/1‑251 (MQ=255)                            gTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATg                                                                                                                                                                                                         <  1:323877/251‑1 (MQ=255)                               gTCGCAAAGTTTGCCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCg                                                                                                                                                                                                       <  2:259680/250‑1 (MQ=255)                                             cgcgTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGt                                                                                                                                                                                         <  1:56820/250‑1 (MQ=255)                                                gTGGCAAATGGGGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCtt                                                                                                                                                                                     <  2:80453/251‑2 (MQ=255)                                                                    tcTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGGCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCGCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGttt                                                                                                                                                                  <  1:117085/249‑1 (MQ=255)                                                                    ccTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCATGAGACGATCCATCTGGCCTGCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATACCGTCGATTTAGATCCGAAAGCCGACGCTCCCGATCATTAAGAACTTGCAAAGAGCAGTCCGGACGATCGGtt                                                                                                                                                                                                                                      <  1:318276/182‑1 (MQ=255)                                                                               tGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGATCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCAAGGCCGGATGCCGTCGCTTTAGATCCGACAGCCGACTCTCCCGATCATGAAGCACTTGCAAAGAGCATTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGCGGCTGTTGAGGGTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCt                                                                                                                                                      >  2:298112/1‑251 (MQ=255)                                                                                    gggTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGa                                                                                                                                                  <  1:121194/250‑1 (MQ=255)                                                                                        tGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTc                                                                                                                                              <  2:263448/250‑1 (MQ=255)                                                                                               cATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGTTTTGAAGTCATTGTTCt                                                                                                                                      <  1:102549/251‑1 (MQ=255)                                                                                                          aaTGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGCTTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTACGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCGTTGTTCTGGATGTTTTgt                                                                                                                           >  2:143156/1‑250 (MQ=255)                                                                                                          aaTGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGc                                                                                                                           >  2:293817/1‑251 (MQ=255)                                                                                                                        agaCGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTAt                                                                                                             <  2:42996/251‑1 (MQ=255)                                                                                                                                        cTTCGCCTTTGTGAATGTCAAAATTACGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGCGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGACATCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGGCCCCGtgatg                                                                                             >  2:213938/1‑251 (MQ=255)                                                                                                                                          tCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGTACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCTTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCATGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGtgatg                                                                                             <  1:56426/249‑1 (MQ=255)                                                                                                                                                       tGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGCGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCgttgtt                                                                              >  2:114049/1‑247 (MQ=255)                                                                                                                                                         tCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGc                                                                            >  1:309646/1‑251 (MQ=255)                                                                                                                                                               ttAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTAt                                                                                                             <  2:75439/212‑1 (MQ=255)                                                                                                                                                               ttAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTAt                                                                                                             >  1:75439/1‑212 (MQ=255)                                                                                                                                                                          ccGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAGCTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGAtggtgg                                                           <  2:316352/251‑1 (MQ=255)                                                                                                                                                                                   gTCGATTTAGATCCGAAAGCCGACTCTCCTGATCCTGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGCGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGTTTTGCCGCGGGTTGGCGGTACTGGTGGTGGTTGCATCGGTgg                                                  >  2:114839/1‑251 (MQ=255)                                                                                                                                                                                   gTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACGTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCTCCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATgg                                                  <  1:217225/251‑1 (MQ=255)                                                                                                                                                                                         ttAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGGTGGTGGCATCGATGGTGTGat                                            >  1:99224/1‑251 (MQ=255)                                                                                                                                                                                           aGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTGGCAGCGTGTTGGCGGTACTGGAGATGTTGGCATCGATGGTGTGatat                                          <  2:179536/251‑1 (MQ=255)                                                                                                                                                                                               ccGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGATTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTGGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCt                                      <  1:181035/251‑1 (MQ=255)                                                                                                                                                                                                cGAACGCCGACTCTCCCGATCATGAAGAACTTGCCAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGATTTGAAGTCATTGTTCTGGATGTTTTGCCTCGCCTGGGGTATGGCGGCCACCGTGATGATTTGACGCGTGTTGTCGGGACTGGTGATGGTGGCCTCGATGGGGTGATCTCGCtt                                     >  2:133424/1‑251 (MQ=255)                                                                                                                                                                                                cGAAAGCCGTCACTCCCGATCATGAAGAACTTGCACCGCGCAGTCCGGACGATCGCTTAGCTCAGGCGCTAACAGAGCTTCGAGATGCGGTGGCTGATGCGGTTCTGGAAAAATTATTGCAGGTTTCTCATTCGCGCTTTGAAGTCATTGTTCTGGCTGCTTTGCATCGCCTGGGGTATGGCGGCCACCTCGATGTTTTGCCGAGGGTTTGCGGTACGGGAGATGGTGGCATCGATGGGGGGATATCGCtt                                     >  2:133417/1‑251 (MQ=255)                                                                                                                                                                                                cGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCtt                                     <  1:12654/251‑1 (MQ=255)                                                                                                                                                                                                     gCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGATTCGTGATGCGGTGGCTGCTGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGACACCGTGATGATTTGGCGCGTGTTGGCGGTACTGGAGATGGGGGCATCGATGGTGTGATATCGCTTggta                                 >  1:354669/1‑247 (MQ=255)                                                                                                                                                                                                        gACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACt                             >  1:333238/1‑251 (MQ=255)                                                                                                                                                                                                           tctcCCGCTCATGAAGAACTTGCAAAGAGCCGTCCGGCCGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTAGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGCGGTGGTGGCCTCGCTGGGGGGATATCGCTTGATAAACTTgg                          >  2:322537/1‑251 (MQ=255)                                                                                                                                                                                                            ctcCCGATCATGAAGAACTTGCCAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGCTGGTGGCATCGATgg                                                  >  1:287487/1‑226 (MQ=255)                                                                                                                                                                                                            ctcCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTg                           <  1:223885/249‑1 (MQ=255)                                                                                                                                                                                                            ctcCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCGTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATgg                                                  <  2:287487/226‑1 (MQ=255)                                                                                                                                                                                                            cgcccGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGGGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTTGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGAGGGTGTGATATCGCTTGATAATCTTgg                          <  2:5837/248‑1 (MQ=255)                                                                                                                                                                                                              cccGATCATTAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGATCTTCGTGATGCGGTGGCTGATGAGGTGCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTTGATGTTTTGCATCGCCTGGGGTAGGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTgg                          <  1:130968/248‑1 (MQ=255)                                                                                                                                                                                                                         agaaCTTGAAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGttt            <  2:137172/251‑1 (MQ=255)                                                                                                                                                                                                                                   aaGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGc  <  2:105567/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   GGCGCATGACCGTTTAAAACGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGCCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTATGTTCAGGC  >  NC_000913/4587131‑4587607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑