Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,571,280	C→T	E99K (GAG→AAG)	yjiR ←	fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,571,280	0	C	T	100.0%	75.5 / NA	27	E99K (GAG→AAG)	yjiR	fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/23);  total (4/23)

TGATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTT‑ACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACA  >  NC_000913/4571061‑4571519                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                 ggatgTCATCGGCGGTGACCCCCGAGCCGCTGTCGAGCATCAGGCGGGAAACCGGCTCTCGCATTACTCTCTGACCGGCAAACAAGTCATACCCGAGAACGGTTGGCAGATTATGCTGCCCCACCCGGCTTAACTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTa                                                                                                                                                                                                                   <  2:151777/250‑1 (MQ=255)   atgTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAAt                                                                                                                                                                                                                 <  1:158044/251‑1 (MQ=255)        accggcggTGCCCCCCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTTCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCa                                                                                                                                                                                                            <  1:289398/249‑1 (MQ=255)         ccgacggtcccccccGAGCCGCTGTCGAGCATCAGGCGGGCAACCGGCTCTCGCAATCCTCGCTGACCGCCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGCCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCTCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCAc                                                                                                                                                                                                           <  2:325481/238‑1 (MQ=255)                           ccGCTGTCGAGCATGAGGCGGGCAATCTGCTCTCGCAATACGCGCTGACCGGCTAACAAGTCATACCCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGAcg                                                                                                                                                                                         <  1:275539/251‑1 (MQ=255)                                gTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCAAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCCCCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCa                                                                                                                                                                                    <  1:264771/251‑1 (MQ=255)                                       aTCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATcggcgg                                                                                                                                                                             >  1:264383/1‑251 (MQ=255)                                            gCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTg                                                                                                                                                                        <  1:116116/251‑1 (MQ=255)                                              gggCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTggc                                                                                                                                                                      <  1:118355/251‑1 (MQ=255)                                                       gCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGGTggggg                                                                                                                                                             >  1:302673/1‑249 (MQ=255)                                                              cAATACTCGCTGACCGGCTCACAAGTCATAACCGACAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTACCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGGACTGGCGGTCGGGCTTT‑Acatt                                                                                                                                                     >  2:146857/1‑248 (MQ=255)                                                                           ccGGCTAACAAGTCATAACCGAGAACGTTTTGCAGATTATGCTGCCCCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGTGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTT‑ACGTTGTGCGACAAAATa                                                                                                                                        <  1:151906/251‑1 (MQ=255)                                                                                        aatatccGAAAACGGTTGGCAGATTATGCGGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTGTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTT‑ACGTTGTGCGACAAAATAACCCGAACGCGGc                                                                                                                           <  1:147886/246‑1 (MQ=255)                                                                                            cccagaacgtgtgtGCAGATTTGGCTGCACCCCCCGGCTGAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGGCATCGGCGGTACTGGCGGCTGGGCTTT‑ACGTTGTGCGACAAAATAACCCGAACGCGGCTGCg                                                                                                                       <  2:330677/238‑1 (MQ=255)                                                                                               gagaACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTTAGGTT‑TGGGGGGAAATAACCCGAAAGCGGCTGCgggg                                                                                                                    >  1:78446/1‑249 (MQ=255)                                                                                                   aCGGTTTGCAGATTATGCTCCACCCCCCGGCTTAGCCCCCGCCACAGCGGTTGCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGTAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTT‑ACGTTGTGCGACAAAATAACCCGAACTCGGCTGCGGAGtgat                                                                                                                <  2:40799/251‑1 (MQ=255)                                                                                                          gCAGATTATGCTCCGCCGCCCGTCTTGGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGAGCCCACTGGGTAATTGCCACCGGGCGCTGCACCGGACGCGTCATCGGCTGTACTGGCGGCTGGGCTTT‑ACGTTGTGCGACAAAATAACCCGAACGCGGCTTCGGAGTGATGAGCtt                                                                                                          <  2:182218/250‑1 (MQ=255)                                                                                                                      accacccacggcccctcTCCCGCCCCAGCGGTTTCAGGCTGGGCGCTTCGACTTCCGGTGTGCTTTTGCTTAACGGACCAATTGAACTGTCGCTATGCGCCTTCAGTATATCCAGCTCCTGATCCCACTGGGTAATTTCCCCTGGGCGCTGCACGGGACGCGTCATCGGCGTTACTGGCTGCTGGGCTTT‑ACTTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTTATCTTCTCCAg                                                                                              <  2:25570/234‑1 (MQ=255)                                                                                                                                    accccccGCTACAGCGGGTTCAGGCGTGGCGTGTCGACATCCGGTGTGCCTGTGCTTAACGGATCAATGGAACTGTCGCTATGCGCCGTCAGCATATCCCGCACCGGATCCCACTGGGTAATTTACACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTT‑ACGTTGTGCGACAAAATAACCCGAACGCGGCTTCGGAGTTATGAGCTTCAGCGTCTTCAGCGTCTGATACGCctg                                                                               <  2:260434/247‑1 (MQ=255)                                                                                                                                               ccgCGGTTTCAGGCGGGGCGCTTCGCCATCCGGTGTGCTTTTGCTGAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCGGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTT‑GCGTTGTGCGACAAAATAGCCCGAACGCGGCTGCGGAGTGATGATCTTCATCGTCTCCAGCGTCGGATACGCCGGCTGCACGGTGc                                                                    <  2:26945/249‑1 (MQ=255)                                                                                                                                                      ttCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTT‑ACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATAc                                                              <  2:302673/250‑1 (MQ=255)                                                                                                                                                         aGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTT‑ACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGAc                                                          <  2:76615/251‑1 (MQ=255)                                                                                                                                                                       gACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTT‑ACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCGGCTGCGCGGTGCTGATACTTACGCCGTGCCCCTGAc                                            <  2:34480/251‑1 (MQ=255)                                                                                                                                                                        aCATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTT‑ACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACt                                           <  1:11923/251‑1 (MQ=255)                                                                                                                                                                                                 gAACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTT‑ACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTtc                  <  1:106723/251‑1 (MQ=255)                                                                                                                                                                                                    cAATGGAACTGTCGCTATGCGCCTTCAGCATAGCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCGGCACCGGACGCGTCAGCGGCGGTACTGGCGGCTGGGCTTT‑ACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATTCTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTcc               <  2:223888/251‑1 (MQ=255)                                                                                                                                                                                                                 gCTATGCGCCTTCAGCAGATCCAGCACCTGATCCCTCTGGGTAATTTCCTCCGGGCGCTGCACCGGACGCGGCATCGTCGGTACTTGCGGCTGGGCTTT‑TCGTTGTGCGACAAAATAACCCGAACGCGGCTGCTGAGTGAGGAGCTTCAGCGTCTCCAGCGTCTGATGCGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTGCTGACTTAAGCTGCGCGCCGACGGCAATTTCGCCCCGTGGGGATACa  <  2:320529/251‑1 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                 TGATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGGTTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGGAACAATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTT‑ACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACA  >  NC_000913/4571061‑4571519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑