Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,570,550	C→T	S342N (AGC→AAC)	yjiR ←	fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,570,550	0	C	T	97.0%	100.8 / NA	33	S342N (AGC→AAC)	yjiR	fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (17/15);  minor base A (1/0);  total (18/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

TTTCATGCGGCACAGCTGCCGCGCGACGCAGACCATATCGACCTGTTCAGGCAATTCTATCCACAGTAAAAATCCGCCTTTCGGGCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGCTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCCAGGAATGAATGGTCCGCGGGCGCGGATACTCCGTCGCCAGTTCGCCATAGACATCATCTTCAAAAATCACAATATCATGACGCTGAGCGAGAGAGAGAACGGCCCGTTTGCG  >  NC_000913/4570306‑4570791                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  tttCATGCGGCACAGCTGCCGCGCGACGCAGACCATATCGACCTGTTCAGGCAATTCTATCCACAGTAAAAATCCGCCTTTCGGGCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCAAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACCTTAAAGttgctg                                                                                                                                                                                                                                              >  2:10754/1‑250 (MQ=255)     aTGCGGCACAGCTGCCGCGCGACGCAGACCATATCGACCTGTTCAGGCAATTCTATCCACAGTAAAAATCCGCCTTTCGGGCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGc                                                                                                                                                                                                                                         >  1:13370/1‑251 (MQ=255)       gCGGCAAAGCTGCCGCGCGACGCAGACCATATCGACCTGTTCAGGCAATTCTATCCACAGTAAAAATCCGCCTTTCGGGCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGt                                                                                                                                                                                                                                       <  2:160319/251‑1 (MQ=255)               gCTGCCGCGCGACGCAGACCATATCGACCTGTTCAGGCAATTCTATCCACAGTAAAAATCCGCCTTTCGGGCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAATTTGCTGATGGCGTATTTCat                                                                                                                                                                                                                                >  1:322910/1‑250 (MQ=255)               gCTGCCGCGCGACGCAGACCATATCGACCTGTTCAGGCAATTCTATCCACAGTAAAAATCCGCCTTTCGGGCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCata                                                                                                                                                                                                                               <  1:105907/251‑1 (MQ=255)                      cgcgACGCAGACCATATCGACCTGTTCAGGCAATTCTATCCACAGTAAAAATCCGCCTTTCGGGCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCa                                                                                                                                                                                                                        <  2:160421/251‑1 (MQ=255)                                                cAGGCAATTCTATCCACAGTAAAAATCCGCCTTTCGGGCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCg                                                                                                                                                                                              >  2:73003/1‑251 (MQ=255)                                                        tCTATCCACAGTAAAAATCCGCCTTTCGGGCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGacccaac                                                                                                                                                                                       <  2:221854/250‑1 (MQ=255)                                                             ccACAGTAAAAACCGGCCTTCCGGGGGCGTAAAACAGATTTGGCAGGGAAAATATTCCCGTATCCGGCAGGTAAAAAGCGCCAAATGGCGCTGATAAATCTGCCGCATCCGCCGAATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTCCGTGGACGGCACATTAA‑GTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCAcgc                                                                                                                                                                                 <  1:251567/250‑1 (MQ=255)                                                                   tAAAAATCCGCCTTTCGGGCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTAt                                                                                                                                                                                                                                     <  2:267769/193‑1 (MQ=255)                                                                   tAAAAATCCGCCTTTCGGGCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTAt                                                                                                                                                                                                                                     >  1:267769/1‑193 (MQ=255)                                                                         cccGCCTTTCGGGCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTg                                                                                                                                                                     <  2:329041/250‑1 (MQ=255)                                                                          ccGCCTTTCGGGCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGc                                                                                                                                                                    <  1:141823/251‑1 (MQ=255)                                                                           cGCCTTTCGGGCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGc                                                                                                                                                                    >  2:6331/1‑250 (MQ=255)                                                                              cTTTCGGGCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAAt                                                                                                                                                                 >  1:302941/1‑250 (MQ=255)                                                                                   gggCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACtttt                                                                                                                                                           >  1:282092/1‑251 (MQ=255)                                                                                                           ggAAACTATTCCCGTATCCAGCAGGTATACAGCGCCAAATTGCGCTGATAGATCTGCCGCATACCCCGGGTATGGCGACGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACCTTCAAGTTGCTGATGGCGAACTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGGCCCATACTTTTACTGACCGAGCTGCACAACCGCAc                                                                                                                                   >  2:326498/1‑251 (MQ=255)                                                                                                                             cagcagGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCCa                                                                                                                 <  1:6331/251‑1 (MQ=255)                                                                                                                                        aaGCGCCCAATTGAGATGATAGATCAGACGCATCCGCCGGATATGGCGATGATAGTGACCTTCCCGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGATGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATCACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCACGGAATGAATgg                                                                                                      >  2:205710/1‑251 (MQ=255)                                                                                                                                            gcCAACTTGCGCTGATAGCTCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGCGCTGCACAACCGCACTCGCCCGTCGATATCCCAGGAATGAATGGTCcg                                                                                                  >  2:141802/1‑251 (MQ=255)                                                                                                                                                aaTTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGCCCTACCAGCTCAAACGTTGCCGCCGCCAGTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTATATCGTGATAACGCCCCGGTGCGGCCCAACCCACGCGCAGGCCTGGTGCAATGCTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCCAGGAATGAATGGTCCGCggg                                                                                              <  2:11038/251‑1 (MQ=255)                                                                                                                                                            aGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCCAGGAATGAATGGTCCGCGGGCGCGGATACTcc                                                                                  >  1:184447/1‑251 (MQ=255)                                                                                                                                                                  gCCGCATCCGCCGGATATGACGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGAGACCCAACCCACGCGCAGGCCTGGTGCACTACTTTTAATGAACGAGCTGCACAACAGCACTCGCCCGTAGCTTTCCCAGGAATGAATGGTCCGCGGGAGCGGATACTACGTCGcc                                                                            >  1:119784/1‑251 (MQ=255)                                                                                                                                                                    cGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGAATTTCATATGCCTCAGTTTCTCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCCAGGAATGAATGGTCCGCGGGCGCGGATACTCCgccgcc                                                                            >  2:346091/1‑249 (MQ=255)                                                                                                                                                                      cATCCGCCGGATATGGCGATGATAGTGACCTCCCAGCACAAACGTTGACGCCGCCATTTGCGTGTACGGCACATTAAAGTTGCTGATGGCGGATTTCATATGCATCAGTTTATCGTGATAACGCCCTGGTGCGAGCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCGGCACAGCAGCACTCTCCCGTCGAGATCCCAGGAATGAATGGTCCGCGGGCGCGGATACTCCGTCGCCAGtt                                                                        <  1:87546/251‑1 (MQ=255)                                                                                                                                                                       aTCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTGGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGGTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCCAGGAATGAATGGTCCGCGGGCGCGGATACTCCGTCGCCAGTTc                                                                       <  1:349715/251‑1 (MQ=255)                                                                                                                                                                         ccgccgGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATTGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCCAGGAATGAATGGTCCGCGGGCGCGGATACTCCGTCGCCAGTTCg                                                                      <  2:184447/250‑1 (MQ=255)                                                                                                                                                                                  atGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCCAGGAATGAATGGTCCGCGGGCGCGGATACTCCGTCGCCAGTTCGCCATAGAca                                                             <  1:106206/250‑1 (MQ=255)                                                                                                                                                                                                   cTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCCAGGAATGAATGGTCCGCGGGCGCGGATACTCCGTCGCCAGTTCGCCATAGACATCATCTTCAAAAATcac                                            >  1:103570/1‑250 (MQ=255)                                                                                                                                                                                                                  ttGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCCAGGAATGAATGGTCCGCGGGCGCGGATACTCCGTCGCCAGTTCGCCATAGACATCATCTTCAAAAATCACAATATCATGACGCTg                             >  1:221862/1‑250 (MQ=255)                                                                                                                                                                                                                             aTTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCGTCAGTTTATCGTGATAACGCCCCGGTGCGACACAGCCCACGCGCAGGCCTGGTGCAATACTTTTACTGAAAGAGCTGCACAACAGCACTCGCCCGTCGATATCCCAGGAATGAATGGTCCGCGGGCGCGGATACTCCGTCGCCCGTTCGCCATAGAAATCATCTTCAAAAATCACAATATCATGACGATGAGCgcgagag                   >  1:299369/1‑249 (MQ=255)                                                                                                                                                                                                                              tttGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCCAGGAATGAATGGTCCGCGGGCGCGGATACTCCGTCGCCAGTTCGCCATAGACATCATCTTCAAAAATCACAATATCATGACGCTGAGCgagagag                   >  1:202061/1‑248 (MQ=255)                                                                                                                                                                                                                                           cacaGTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTTATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGGGCAATACTTTTACTGAACGAGCTGCACAACAGCACGCGCCCGTCGATAGCCCAGGAATGAATGGTCCGCGGGCGCGGATACTCCGTCGGCGGTTCGGCATAGACATCATCTGCAAAAATCACAATATCATGACGCTGAGCGAGAGAGAGAGCGGCCTGTTTgc   <  2:300496/251‑1 (MQ=255)                                                                                                                                                                                                                                            acaTTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCCAGGAATGAATGGTCCGCGGGCGCGGATACTCCGTCGCCAGTTCGCCATAGACATCATCTTCAAAAATCACAATATCATGACGCTGAGCGAGAGAGAGAACGGCCCGTTTgcg  >  2:154505/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  TTTCATGCGGCACAGCTGCCGCGCGACGCAGACCATATCGACCTGTTCAGGCAATTCTATCCACAGTAAAAATCCGCCTTTCGGGCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGCTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCCAGGAATGAATGGTCCGCGGGCGCGGATACTCCGTCGCCAGTTCGCCATAGACATCATCTTCAAAAATCACAATATCATGACGCTGAGCGAGAGAGAGAACGGCCCGTTTGCG  >  NC_000913/4570306‑4570791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑