Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,533,518	C→T	S234F (TCC→TTC)	pck →	phosphoenolpyruvate carboxykinase (ATP)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,533,518	0	C	T	100.0%	177.2 / NA	52	S234F (TCC→TTC)	pck	phosphoenolpyruvate carboxykinase (ATP)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/21);  total (31/21)

TTATTCGCCCGAGCGATGAAGAACTGGCAGGTTTCAAACCAGACTTTATCGTTATGAACGGCGCGAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTCCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAAAGAAGCGGAACCTGAAATCTACAACGCTATCCGTCGTGATGCGTTGCTGGAAAACGTC  >  NC_000913/3533272‑3533756                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                ttATTCGCCCGAGCGATGAAGAACTGGCAGGTTTCAAACCAGACTTTATCGTTATGAACGGCGCGAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGCAGAAAGGGATGTTCTCGGTGATGAACTACCTGCTGCCGCTGGAAGGTAGCGCTTCTATGAACTGCTTCGcc                                                                                                                                                                                                                                             >  2:203369/1‑251 (MQ=255)      cGCCCGAGCGATGAAGAACTGGCAGTTTTCAAACCAGACTTTATCGTTATGAACGGCGCGAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGt                                                                                                                                                                                                                                        <  2:234895/251‑1 (MQ=255)       gCCCGAGCGATGAAGAACTGGCAGGTTTCAAACCAGACTTTATCGTTATGAACGGCGCGAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGAGTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGtt                                                                                                                                                                                                                                       <  2:79221/251‑1 (MQ=255)              cGATGAAGAACTGGCAGGTTTCAAACCAGACTTTATCGTTATGAACGGCGCGAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTgaga                                                                                                                                                                                                                                <  2:117832/251‑1 (MQ=255)                     gaaCTGGCAGGTTTCAAACCAGACTTTATCGTTATGAACGGCGCGAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACGTCGTGGCGTGTAACCGGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTAGCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGa                                                                                                                                                                                                                         <  2:271292/251‑1 (MQ=255)                        cTGGCAGGTTTCAAACCAGCCTTTATCGTTATGAACGGCGCGAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAAGGATCGCTTCTATGCCCTGCTTCGCCAACGCTGGGGGGAAAGGCGTTGt                                                                                                                                                                                                                      >  2:113820/1‑251 (MQ=255)                            cAGGTTTCAAACCAGACTTTATCGTTATGAACGGCGCGAAGTGCACTAACCCGCAGTGGCAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAGGAAAAGGATGTTCTCGGTGATGAACTACCTGCTGCCGCTGCAAGGGATAGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCTATGGTGCg                                                                                                                                                                                                                  >  1:213357/1‑251 (MQ=255)                               gTTTCAAACCAGACTTTATCGTTATGAACGGCGCGAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGgtg                                                                                                                                                                                                               >  1:99547/1‑251 (MQ=255)                                       ccAGACTTTATCGTTATGAACGGCGCGAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGGGTTCTTCgg                                                                                                                                                                                                       >  1:116528/1‑251 (MQ=255)                                                   gTTATGAACGGCGCGAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCAc                                                                                                                                                                                           <  1:19137/251‑1 (MQ=255)                                                        gcaCGGCGCGAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGt                                                                                                                                                                                      >  2:91332/3‑250 (MQ=255)                                                          aCGGCGCGAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACATGAACGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGCAGAAAGGGATGGTCTCGATGATGAACTACCGGCTGCCGCTGAAAGGTATCGCTTATATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGGTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTaa                                                                                                                                                                                    >  1:213328/1‑250 (MQ=255)                                                                 gAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTACGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGTGACATGAAGACAGGGGTGTTCTCGATGATGAACTACCTGCTGCCGCTGCAAGGTATAGATTTTATGCACTGCTTCGCCAACGCTTGTGGGAGATGCGATGTGGCGGTGGTCTTCGGCATTTCCGGCACC‑GGTAAAACAAccc                                                                                                                                                                            >  2:274185/1‑251 (MQ=255)                                                                     tGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGGATCGCTTCTATGCAATGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGAGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAAACACCCTTTc                                                                                                                                                                        >  2:336105/1‑251 (MQ=255)                                                                          tAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGCAGAAAGGGCTGTTCTCGATGGTGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCCCC‑GGTAAAACCCCCCTTTCCACCg                                                                                                                                                                   >  2:45042/1‑251 (MQ=255)                                                                                   gTGGAAAGAACAGGGTCTCAACTACGAAAACTTCGTGGCGTTTAACCAGACAGAGCGAATGCAGCTGATTGGCGGCAACTGGTACGGCGGCGGAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCAGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGGGGGAAAGGCGATGTTGCGGTGTTCTTTGGCCTTTTAGG‑ACCAGGTAAAACCACCCATTCCACCGCCCAGAAAc                                                                                                                                                          >  2:204365/1‑251 (MQ=255)                                                                                             cAGGGTCTCAACGCCTACAACTTCTGGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGAACCTGGTTCGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGACGTTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCGGAt                                                                                                                                                <  2:268134/251‑1 (MQ=255)                                                                                                 gTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGc                                                                                                                                            <  1:336105/251‑1 (MQ=255)                                                                                                             aaaaCTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCTCCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAacac                                                                                                                                <  1:204365/251‑1 (MQ=255)                                                                                                              aaaCTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGGTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGGATCGCTTCTATGCACTGCTTAGCCCACGTTGGGGAGAAAGGCGGTGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑CGTAAAACCACCCTTTCCACCGACCCGAACCGGCGCCTGATTGGCGagtaagaacccg                                                                                                                               >  2:113276/1‑240 (MQ=255)                                                                                                                      tGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGCAAGAGATGTTCTCCATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGACTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAAAGTCGCCTGATTGGCGATCACGAACACGGCTGGGAc                                                                                                                       >  1:294138/1‑251 (MQ=255)                                                                                                                        gCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGGTGACGAACACGGCTGGGACg                                                                                                                      >  1:328282/1‑250 (MQ=255)                                                                                                                             tAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGCTGAACTACCTGCTGCCGCTGAAAGGTATACCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCAAC‑GGTTAAACCACCCTTTCCACCGACCCGAAACGACGCCTGATTGGCGATGACGAAAACGGCTGGGACGATGACg                                                                                                                >  1:155523/1‑251 (MQ=255)                                                                                                                                ccTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCg                                                                                                             <  1:27840/251‑1 (MQ=255)                                                                                                                                  tGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGGAATGGAGAAAGGGATGTTCTCGATGATGAACTACCTGCCGCCGCTGAAAGGAATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGttct                                                                                                                                                                                                           >  2:246378/1‑156 (MQ=255)                                                                                                                                  tGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGGTGGTGAGAAAGGCGATGTTGCGGTGttct                                                                                                                                                                                                           <  1:246378/156‑1 (MQ=255)                                                                                                                                    aCCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGtt                                                                                                         <  1:299966/251‑1 (MQ=255)                                                                                                                                                  gCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAggcg                                                                                           <  1:61517/251‑1 (MQ=255)                                                                                                                                                     gagtGGCGGCACCTGGTCCGGCGGCGAAAGGAAGAAAGGTATGTTCTCGATGATACACTACCTGCTGGTGCTGAAAGGTATCGCTTTGATGCTGTGCTTCGCTAACGGTGTTGAGAAAGGCGATGTTGCTGTGTTCTACGGCCTTTCCTGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTCGCGATGACGAACACGGCTGGGACGATGACGGCTTGTTTAACTTCGAAGGCGgct                                                                                        <  2:194807/248‑1 (MQ=255)                                                                                                                                                        tGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGgctgc                                                                                      <  1:320975/250‑1 (MQ=255)                                                                                                                                                          gcggcACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTAc                                                                                   <  2:111574/251‑1 (MQ=255)                                                                                                                                                                ccTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGGAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCaca                                                                              >  2:70822/1‑248 (MQ=255)                                                                                                                                                                 cTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCaaaa                                                                             >  1:312984/1‑250 (MQ=255)                                                                                                                                                                          cggcgAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAACCTATCAAGc                                                                   >  2:45109/1‑251 (MQ=255)                                                                                                                                                                           ggcgAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCt                                                                  <  2:128467/251‑1 (MQ=255)                                                                                                                                                                             cgAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGTCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCGCCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGt                                                                <  1:173723/251‑1 (MQ=255)                                                                                                                                                                               aaaTGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGTGGCTGCTACGCAAAAACTATCAAGCTGTCg                                                              <  1:166906/251‑1 (MQ=255)                                                                                                                                                                               aaaTGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTggcg                                                              >  2:213858/1‑248 (MQ=255)                                                                                                                                                                                aaTGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCg                                                              >  1:2220/1‑250 (MQ=255)                                                                                                                                                                                     agaaAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAaagaa                                                        <  1:203369/251‑1 (MQ=255)                                                                                                                                                                                      gaaAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGGTTAACTTCGAAGGCGGCTGCCACGCAAAAACTAACAAGCTGTCGAaaga                                                         >  2:298869/1‑249 (MQ=255)                                                                                                                                                                                        aaGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAAAGAAGCg                                                     >  1:168185/1‑251 (MQ=255)                                                                                                                                                                                            gATGTTCTCGATGATGGACTACCTGCTTCCGCTTAAAGGTATCGCTTCTATGCCCTGCTTCGCCAACGGTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCGGAGTGGCGATGACGAACACGGCTGTGACGATGACGGCGGGTTTTACTTCGAAGGCGGCGGCTACGCAAAAGTTATCAAGCTGTCGAAATATGCGGAAc                                                 <  2:231170/251‑1 (MQ=255)                                                                                                                                                                                               gTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAAAGAAGCGGAACCTg                                              <  1:283581/251‑1 (MQ=255)                                                                                                                                                                                                      atgatgACCTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTCGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAAATTCCAAGACGCCTGCTAAGCGAAAACTATCAACCAGTCACAAGAAGCGGCACCtagaatctc                                       >  2:137067/1‑243 (MQ=255)                                                                                                                                                                                                            aaCTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGCTGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTTGCGAAAGAAGCGGCACCTGAAATCTACCACGc                                 >  2:302086/1‑251 (MQ=255)                                                                                                                                                                                                                               aaGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAAAGAAGCGGAACCTGAAATCTACAACGCTATCCGTCGTGATGCGTTg              >  1:48825/1‑251 (MQ=255)                                                                                                                                                                                                                               aaGGTATCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAAAGAAGCGGAACCTGAAATCTACAACGCTATCCGTCGTGATGCGTTg              >  1:94933/1‑251 (MQ=255)                                                                                                                                                                                                                                    aTCGCTTCTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAAAGAAGCGGGACATGCAATCTACAACGCTATCCGTCGTGATGCGTTGCTgg          >  1:178243/1‑250 (MQ=255)                                                                                                                                                                                                                                           cTATGCACTGCTTCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAAAGAAGCGGAACCTGAAATCTACAACGCTATCCGTCGTGATGCGTTGCTGGAAAACGTc  >  1:244662/1‑251 (MQ=255)                                                                                                                                                                                                                                           atATGCACTGCATCGCCAACGTTGGTGAGCCAGGCGATGTTGCGGTGATCTTCGGCCTTTCCGGCACC‑GGAAAAACCACCCTTTCCACCGCCCCGAAACGTCGCCTGATTGGCGATGACGACCACGGCTGGGACGATGACGGCATGTTTAACTTACACAGCGGCGGATACGCAAAAACTATCAAGCTGTCGCAAGAAGCGGCACCTTAAATCTCCAACGCTATCCATCGTGATGCGTTGCTGGAAAACgcc  >  2:57666/2‑249 (MQ=255)                                                                                                                                                                                                                                            tATGCACTGATTCGCCAACGTTGGAGAGAAAGGCGACGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAAAGAAGCGGAACCTGAAATCTACAACGCTATCCGGCGTGATGCGTTGCTGGAAAACGTc  >  2:22833/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                TTATTCGCCCGAGCGATGAAGAACTGGCAGGTTTCAAACCAGACTTTATCGTTATGAACGGCGCGAAGTGCACTAACCCGCAGTGGAAAGAACAGGGTCTCAACTCCGAAAACTTCGTGGCGTTTAACCTGACCGAGCGCATGCAGCTGATTGGCGGCACCTGGTACGGCGGCGAAATGAAGAAAGGGATGTTCTCGATGATGAACTACCTGCTGCCGCTGAAAGGTATCGCTTCTATGCACTGCTCCGCCAACGTTGGTGAGAAAGGCGATGTTGCGGTGTTCTTCGGCCTTTCCGGCACC‑GGTAAAACCACCCTTTCCACCGACCCGAAACGTCGCCTGATTGGCGATGACGAACACGGCTGGGACGATGACGGCGTGTTTAACTTCGAAGGCGGCTGCTACGCAAAAACTATCAAGCTGTCGAAAGAAGCGGAACCTGAAATCTACAACGCTATCCGTCGTGATGCGTTGCTGGAAAACGTC  >  NC_000913/3533272‑3533756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑