Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,871,153	T→G	N51T (AAC→ACC)	truD ←	tRNA pseudouridine(13) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,871,153	0	T	G	100.0%	98.8 / NA	29	N51T (AAC→ACC)	truD	tRNA pseudouridine(13) synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (19/10);  total (19/10)

GGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTT‑GCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTGGCTTTCAC  >  NC_000913/2870909‑2871396                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    ggTAAAGGCGTTCCATTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTT‑GCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTg                                                                                                                                                                                                                                                >  2:240202/1‑250 (MQ=255)   tAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTT‑GCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAgg                                                                                                                                                                                                                                             >  2:132468/1‑251 (MQ=255)    aaaGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTT‑GCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAggt                                                                                                                                                                                                                                            >  1:93786/1‑250 (MQ=255)     aaGGCATTACCTTTAAGCGCGCCTACACGCAGCTTGCGCTTGTGCCGCGCATACTCCCGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCCTAACCACTGTTCCGTAACAGCATGTTTGTCTTTTT‑GCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCAAGTGCATCCGCAACAAACCGGGTATTGCAGCCGGTTTTGAGGAt                                                                                                                                                                                                                                           >  2:328446/1‑251 (MQ=255)                    ggcgcgcaaaaaCGCAGCTTGCGTTGGTGCCGCGCATACTCCAGCCCCTGGCCGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTCCCTTGCCGGGCACGGGAGCGCATACCCCCTTTCCCGTAACAGCTTTTTTGTCTTTTG‑GCCCAGCGAGGCTGACTTCACGGGCATGAATTTTCAGAAATTCCGCCAGTCCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATg                                                                                                                                                                                                                            <  2:72829/242‑1 (MQ=255)                      cgcgcCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGAGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTT‑GCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCt                                                                                                                                                                                                                          >  1:64896/1‑251 (MQ=255)                               aCGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTT‑GCCCAGCGAAGCTGCCTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGGTTTGAGGATTCTAACCAGAATATGCTCACCTTCAc                                                                                                                                                                                                                 >  2:186511/1‑251 (MQ=255)                                               tgCCGCGCATACTCCAACACCTGGCAGCCTTCCAGTTAAAAGTCGCTCAGATCGGGCATTTCCTTGCCCTGCACGCGAGCGCATAACCACTGTTCCGTAACAGAATGTTTGTCTTTTT‑GCCCAGCGAAGCTCACTTCACGGGCATGAATTTTCAGGCATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGATGATTCTAACCCGAATATGCTCACCTTCACCATCAGGCTCAAGGcc                                                                                                                                                                                                 >  2:4/1‑251 (MQ=255)                                                   gcgcATACTCCAGCCCCTGGCAGCCTTCCAGTTGAAAGCCGCTCAGAGCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGAAACAGCATTTTTGTCTTTTG‑CCCCAGCGAACCTGACTTCACGGGCATTAATTTGCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCaaa                                                                                                                                                                                             <  1:200102/251‑1 (MQ=255)                                                      cATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTT‑GCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCt                                                                                                                                                                                          >  1:289298/1‑251 (MQ=255)                                                             cAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCCCGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTT‑GCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTccacc                                                                                                                                                                                    >  2:309295/1‑250 (MQ=255)                                                                     gccaccttcccAGTTGAAAGGCTCTCAGATCGGGCTTTTCCTTGCGGGTCACGCGAGCGCATAACCGCGTTTCCGTAACAGCATGTTTTTCTTTTT‑GCCCATCGAATCTAACTTCGCGGGCATGAATTTCGAGTAATTTCGCCAGGGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCGCCACAAAg                                                                                                                                                                           <  2:165101/242‑1 (MQ=255)                                                                             tcccgtcgAAAGGCGCTCAGATAGCGCATTTCCTTGCCCGACACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTT‑GCCAAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAACCGGGTATTGCCGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCCTCAGGCTCAAAGCCCAACTCTTACACCCCCACACAGTCTTACgg                                                                                                                                                                   >  2:31803/8‑251 (MQ=255)                                                                                                                gCCCGGCCCCCGAGCGCGTACCCACTGTTCCGGAACAGTATTTTGGTCTTTTT‑CCCCAGTGAAGCTGACTTCACGGGCTTGAATTTTCAGGAATTTCGCGAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCGTTCAGCAGCCCGGTGCCTTGCGGTTTAc                                                                                                                                <  2:281605/251‑1 (MQ=255)                                                                                                                         gcgAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTT‑GCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGt                                                                                                                       >  2:94683/1‑251 (MQ=255)                                                                                                                                 atacccccTGTTCCGTAACAGCATGTTT‑TCTTTTTCGCCCGGCGAATCTGCCTTCACTGGCATGAATTTTCAGGAATTTCGCCAGCGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAATCCCAAATCTTCCACCACCGCAAAGTCTTCCGGATTGTCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTgaga                                                                                                               <  1:25889/244‑1 (MQ=255)                                                                                                                                                    aGCATGTTTGTCTTTTT‑GCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATATTCCACCCCCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACAGTGGAGGTAAGTGAGATTATCAAACTCAATCAttt                                                                                            >  1:27231/1‑251 (MQ=255)                                                                                                                                                             gTCTTTTT‑GCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCtt                                                                                   <  1:309295/251‑1 (MQ=255)                                                                                                                                                                                   gactcaacggcaaTGAATTTTCAGTATTTCCGCAAGTTAATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCtt                                                               <  1:16421/238‑1 (MQ=255)                                                                                                                                                                                    aCTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTc                                                              >  1:266909/1‑250 (MQ=255)                                                                                                                                                                                       tcaggggCATGAAGTTTCAGGAGTTGCGCCAGTCCACCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTcaca                                                           <  2:6105/246‑1 (MQ=255)                                                                                                                                                                                       tagagggaagtaaTTTGCAGGAATGTGACCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTGTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCTCAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTcac                                                            <  2:14838/238‑1 (MQ=255)                                                                                                                                                                                         aCGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGc                                                        >  1:5982/1‑251 (MQ=255)                                                                                                                                                                                                   aTTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTcc                                              >  1:12224/1‑251 (MQ=255)                                                                                                                                                                                                       tCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCAc                                          >  1:54485/1‑251 (MQ=255)                                                                                                                                                                                                            aaTTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCg                                     >  2:19081/1‑251 (MQ=255)                                                                                                                                                                                                                            ccGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGttttt                     <  1:238337/251‑1 (MQ=255)                                                                                                                                                                                                                                  caAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACAACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCgt                 >  1:28407/1‑249 (MQ=255)                                                                                                                                                                                                                                               gCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTGGCTTTCAc  >  1:72218/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    GGTAAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCCGTAACAGCATGTTTGTCTTTTT‑GCCCAGCGAAGCTGACTTCACGGGCATGAATTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTGGCTTTCAC  >  NC_000913/2870909‑2871396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑