Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,523,109	G→A	L293L (CTG→TTG)	xapB ←	xanthosine:H(+) symporter XapB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,523,109	0	G	A	100.0%	73.3 / NA	22	L293L (CTG→TTG)	xapB	xanthosine:H(+) symporter XapB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/8);  total (14/8)

ACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCAAAGAGGAAAAAGATG  >  NC_000913/2522880‑2523353                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     aCTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACtcat                                                                                                                                                                                                                                 >  1:185818/1‑251 (MQ=255)    gccatgccaCTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAg                                                                                                                                                                                                                              <  2:29647/251‑1 (MQ=255)       atgccaCTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATAGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCAt                                                                                                                                                                                                                           >  1:300628/1‑251 (MQ=255)                                        acacCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGa                                                                                                                                                                                           >  2:97823/1‑250 (MQ=255)                                                tACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAAGCGCTTTAAAAAGAATGGGATa                                                                                                                                                                                   <  1:241994/250‑1 (MQ=255)                                                         cATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATaa                                                                                                                                                                         <  1:97823/251‑1 (MQ=255)                                                                 gCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGCAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAACGAATGGGATAGTCAGTATAAAGCCCACt                                                                                                                                                                 >  2:292779/1‑251 (MQ=255)                                                                   cccTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTc                                                                                                                                                               >  2:155818/1‑251 (MQ=255)                                                                         gcgcTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCAt                                                                                                                                                         <  1:337687/251‑1 (MQ=255)                                                                             ggggcggaaggCTGTAATCAACCTCCTTGTCGACAAAAACCGAACCAGAAATATAGAATAAAACTAATGCACAGGCATAAACAAGCATCGACAGCAGCAGCAAAATAAATCCGTAGGTGGACGGAGCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCAGGACGGTTTTAATGCCAAATCTCTTGAAAAAGAATGGGATAGTCAGTATAAAGCCCACTGCTTCGATCtgt                                                                                                                                                     <  2:282741/241‑1 (MQ=255)                                                                                   gAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAAt                                                                                                                                                                                                               >  1:240570/1‑187 (MQ=255)                                                                                   gAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAAt                                                                                                                                                                                                               <  2:240570/187‑1 (MQ=255)                                                                                                                 aTACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTcaccac                                                                                                                  >  1:266237/1‑250 (MQ=255)                                                                                                                       aaCCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTg                                                                                                           >  1:119611/1‑251 (MQ=255)                                                                                                                                                   acaGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAAt                                                                               >  1:158789/1‑251 (MQ=255)                                                                                                                                                             aCAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGACTGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGa                                                                      >  1:45878/1‑250 (MQ=255)                                                                                                                                                               aaTCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCCTGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACg                                                                   >  1:178303/1‑251 (MQ=255)                                                                                                                                                                                  aaaaTAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGCCAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTgg                                                 >  2:187605/1‑250 (MQ=255)                                                                                                                                                                                  aaaaTAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTgg                                                 <  1:187605/250‑1 (MQ=255)                                                                                                                                                                                           ccGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACTGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAg                                       <  1:62381/251‑1 (MQ=255)                                                                                                                                                                                             ggTTGTTGACGGCTCGCCATAGGCGAAGAAGCCAAAGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGt                                      >  2:113034/1‑250 (MQ=255)                                                                                                                                                                                                                                aGCGCAACGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATCGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCAAAGAGGAAAAAGATg  >  1:182946/1‑251 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     ACTGCCATGCCACTCAGAATCGAGCCAACCCATGCGCCGACACCATTTACCATGGTCATAAAGAGCCCCTGCGCGCTGGCACGAATGCTGGAATCAACTTCCTGTTCGACAAATACCGAACCAGAAATATTGAAGAAATCGAATGCACAGCCATAAACAATCATCGACAGCAGCAGCAAAATAAATCCGGTTGTTGACGGATCGCCATAGGCGAAGAAGCCAAAGCGCAGCGTCCAGGCCACCATACTCATCAGCATGACGGTTTTAATGCCAAATCGCTTTAAAAAGAATGGGATAGTCAGTATAAAGCCCACTTCTGCCATCTGTGAAACTGACAGTAAAATGGAGGGATATTTCACCACAAAACTGTCAGCAAACTCCGGGTTACGGGCGAAATCATGTAGGAACGGATTACCAAAAACGTTGGTAATTTGCAGTACCGCACCCAGCATCATGGCAAAGAGGAAAAAGATG  >  NC_000913/2522880‑2523353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑