Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,510,609 C→T P327L (CCG→CTG)  yfeO → putative transport protein YfeO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,510,6090CT80.0% 25.9 / ‑5.7 15P327L (CCG→CTG) yfeOputative transport protein YfeO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (10/2);  minor base G (0/3);  total (10/5)
Fisher's exact test for biased strand distribution p-value = 2.20e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

CAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGT‑AACACGCGATGGCTGGTTAAGTCTTTTTATGG‑CGGCAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCT  >  NC_000913/2510386‑2510816
                                                                                                                                                                                                                               |                                                                                                                                                                                                                 
cAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGGTTACTTTTTGCTGGCGGGAATTTAACTTTCCGCCCTGGTCGTTTCTGCCGCCCGGGGGTTTCGCGGGGGGGGGAACTTCCTGGGAGTGttttttggcgggggctttgg                                                                                                                                                                                        >  2:310392/1‑234 (MQ=255)
                        cTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAAATAAACCTGCCGCCCTGGTCGTTGCTGCCCCCAGGGGCTTTTGCGGTGGGCGGATCTTCCTGGCAGTGTTTGTCGGGGGGGCATTTGGGTTGTTGCTGCATGGGCACGGTcc                                                                                                                                                                >  1:54683/1‑251 (MQ=255)
                                       ttttttCTGGGTAGTCGGGGGTTTATTGGTGGCCCGTTTTCGTTGTTAAAGGGGCTGGATGAGATGCAGCAGGTGTTGGAAAAGCAGGTTTTCAGCACCAGCGTTTTTTTTTTGCTGGCGGTAATTAACCTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTGGCGGGGGGCGAATCTTCCTGGCAGTTTTTGCCGGCGTGGTATTAGGGTTGTTGCTGCAGGAGCTGGTTCCCGCCGTACCAGCgg                                                                                                                                                  <  1:310392/246‑1 (MQ=255)
                                        ttATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGc                                                                                                                                                 >  1:293932/1‑250 (MQ=255)
                                           ttctcggttttggggggtttaggggtggACCATTTTCGTTGTTAAAGGGGGTGAAGGAGAGGCAGAAGAGGGTGGAAAATCAGGCTTTCAGCACCAGCGTTTATTTTTTGCGGGGGGTAATAAAACTGGCCGCCTTGGTGGTTGCTCCCGCAAGGGGCTTTCGGGGGGGGCGAATCTTCCGGGCAGTGTTTGTCGGGGTGGCATTAGGGTTGAGGCTGAATGAGCACGTTCCCGCCGTACCAGCGGCAAt                                                                                                                                              <  1:122664/227‑1 (MQ=255)
                                                   aTTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGCGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGGTGCTGCCGCCAGTGGCTTTCGCGGGGGGCGAATCTTCCTGGCCGTGTTTGTCGGCGTGGCATTTGGGGTGTTGCTGCATGAGCACGTTCCCGCCGTACCAGCGGGAATAACCGTTTc                                                                                                                                     >  2:207663/1‑251 (MQ=255)
                                                                                                           gcagtgggtggAAAATCAGGCTTTCAGCACAAGGGATTATTTTTTGCTGGCGATAATAAATCTTGCCGCCCTGGTCTGTGCGGCCGCGAGTGGCTTTCGGGGGGGGAGAATCTTCCGGGCAGTGTTTGTGGGCGTGGTATTAGGGTTGAGGCGGCATGAGCCCTTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGT‑AACACGCGATGGCTGGTTAAGTCttt                                                                            <  1:74267/245‑1 (MQ=255)
                                                                                                           gcagATGGTGGCAACTCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGCAATTAAACTTTCCGCCCTGGGCGGTGATGCCCACAGTGGCTTTCGCGGCGGGGGAATCTTTCTGGCAGTGTTTGTAGGCGAGGCATTTGGGTTGATGCTGCATGAGCACGCTCCAGCCGTACCCGCGGCCATAACCGTTTCTTGCGCTATTCTCGGCATCCGGCTGGTGGT‑AACACGCGATGGCTGGTTAAGGCttt                                                                            >  2:157828/1‑251 (MQ=255)
                                                                                                            cagATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCCGTGTTTGTCGGCGTGGCATTAGGGGTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGGGGG‑AACCCGCGATGGCTGGTTAAGTCtttt                                                                           >  2:40582/1‑251 (MQ=255)
                                                                                                                            aGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTTCCGCCCTGGGCGGTGCTGCCGCCAGTGGCTTTCGCGGCTGGCGAATCCTCCTGGCCGCGTTTGTCGGGGCGGCATTAGGGTTGATGCTGCATGAACACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCGCTTCTCGGCCTCGTGCTGGTGGTGAAC‑CGCGGTGGCTGGGTCAGTATTTTTTTGGTCGGCAgcgct                                                           >  1:16238/1‑247 (MQ=255)
                                                                                                                                   cAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGGCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGGTTGTCGGCGGGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGGGGT‑AACACGCGATGGCTGGTTAAGTCTTTTTATGG‑CGGCAggcggttgtcccc                                                   >  2:87371/1‑239 (MQ=255)
                                                                                                                                                     tttGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCCTCGTGCTGGTGGG‑AACACGCGCTGGCTGGTTAAGTCTTTTTATGG‑CGGGCGTCGTTGTACCCactaacacccttcctcccc                                 >  2:254304/1‑233 (MQ=255)
                                                                                                                                                                    aaaaCTGGCCGCCCTGGCGGTTGCTGCCCCCAGTGGCTTGCGGGGGGGGCGAATCTTCGGGGCTGTGTTTGTCGGCGTGGCATTGGGTTTGATGCTGCATGAGCACGTCCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGT‑AACACGCGATGGCTGGTTAAGTCTTTTTATGG‑CGGCAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCa                  <  1:127009/250‑1 (MQ=255)
                                                                                                                                                                          tGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCTGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGT‑AACACGCGATGGCTGGTTAAGTCTTTTTATGG‑CGGCAGTCGTTGTACCCAATACCACATTTCTACCGCTGCTCTGTATCGTCAttcttc            >  1:85633/1‑251 (MQ=255)
                                                                                                                                                                                    gTCGTTGCTGCCGCCAGGGGCTTTCGGGGTGGGCGAATCTTCCTGGCGGTGTTTGGGGGCGTGGTATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGT‑AACACGCGATGGCTGGTTAAGTCTTTTTATGG‑CGGCAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCt  <  1:207663/251‑1 (MQ=255)
                                                                                                                                                                                                                               |                                                                                                                                                                                                                 
CAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCCGGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGT‑AACACGCGATGGCTGGTTAAGTCTTTTTATGG‑CGGCAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCT  >  NC_000913/2510386‑2510816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑